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   exfoliative toxin 的翻译结果: 查询用时:0.137秒
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exfoliative toxin
相关语句
  表皮剥脱毒素
     Action of Staphylococcal Exfoliative Toxin A on Pemphigus Antigens Expressed on Human Keratinocytes
     表皮剥脱毒素A对天疱疮抗原的影响
短句来源
     Objective To study the action and mechanism of staphylococcal exfoliative toxin A(E-TA)on pemphigus foliaceus antigen(PFA)and pemphigus vulgaris antigen(PVA)expressed on cultured human keratinocytes.
     目的探讨表皮剥脱毒素A(ETA)对体外培养正常人角质形成细胞表面天疱疮抗原的影响及其作用机制。
短句来源
  “exfoliative toxin”译为未确定词的双语例句
     Research on Exfoliative Toxin of pathogenetic Staphylococcus in Cosmetics
     化妆品中致病性球菌表皮剥脱性毒素的研究
短句来源
     [Conclutions]: It indicated some staphylococccus that coagulating enzymes are negative can also produce exfoliative toxin.
     表明化妆品中除金黄色葡萄球菌可产生表皮剥脱性毒素外,其它少数凝固酶阴性葡萄球菌(CNS)① 也可产生上述毒素。
短句来源
  相似匹配句对
     Research on Exfoliative Toxin of pathogenetic Staphylococcus in Cosmetics
     化妆品中致病性球菌表皮剥脱性毒素的研究
短句来源
     Cholera toxin
     霍乱毒素
短句来源
     Phytopathogenic Toxin
     植物病原毒素
短句来源
     Action of Staphylococcal Exfoliative Toxin A on Pemphigus Antigens Expressed on Human Keratinocytes
     表皮剥脱毒素A对天疱疮抗原的影响
短句来源
     Carbamazepine-induced exfoliative dermatitis
     卡马西平致剥脱性皮炎1例
短句来源
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  exfoliative toxin
Genes encoding hemolysin, exfoliative toxin, enterotoxin, toxic shock syndrome toxin-1, and Panton-Valentine leukocidin were found in 6, 4, 0, 0, and 0 strains, respectively.
      
Methicillin-resistant Staphylococcus aureus (MRSA), producing exfoliative toxin B, was isolated from blood and bile cultures, and Aeromonas hydrophila was isolated from bile culture.
      
Isoelectric focusing in the presence of 8 M urea also showed microheterogeneity in exfoliative toxin preparations.
      
Multiple components in two antigenic variants of staphylococcal exfoliative toxin were demonstrated by isoelectric focusing in polyacrylamide gel.
      
Isoelectric focusing patterns of staphylococcal exfoliative toxin
      
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Objective ]: To examine the exfoliative toxin of staphylococcus in cosmetics. [Methods ]: We cultured the different gram positive cocci in protecting skin cosmetics with normal nutrient agar, then making it to mix liquid, injecting 0. 5ml uper liquid into rabbit back after concentyifugation, examining the skin after 24h. The germ that can induce staphylococcal scalded skin syndrom determined by API staphylococcus diagnostic test. [Results ]: Showed that 8 kinds of staphylococcus, such as staphylococcus...

Objective ]: To examine the exfoliative toxin of staphylococcus in cosmetics. [Methods ]: We cultured the different gram positive cocci in protecting skin cosmetics with normal nutrient agar, then making it to mix liquid, injecting 0. 5ml uper liquid into rabbit back after concentyifugation, examining the skin after 24h. The germ that can induce staphylococcal scalded skin syndrom determined by API staphylococcus diagnostic test. [Results ]: Showed that 8 kinds of staphylococcus, such as staphylococcus sciuri, staphylococcus cohnii capitis, haemolyticus, cohnii urealyticum saprophyticusepidermidis and xylosus, 23 strain gram w positive cocci induced staphylococcal scalded skin syndrom besides 7 staphylococcus aureus that we have recognized. [Conclutions]: It indicated some staphylococccus that coagulating enzymes are negative can also produce exfoliative toxin.

为了检测化妆品中产生表皮剥脱性毒素的葡萄球菌,将护肤类化妆品中检出的革兰氏阳性球菌用普通营养 琼脂进行分纯培养,把分纯菌株制成氯化钠肉汤菌悬液,离心后取其上清液0.5ml注射至兔背部皮内,24h后观察其皮 肤反应,将引起皮肤烫伤综合征病变的菌株,再应用API葡萄球菌鉴定试剂盒进行复核鉴定。结果发现除7株金黄色 葡萄球菌外,尚有松鼠、科氏、头状、溶血、腐生、解脲科氏、表皮、木糖葡萄球菌8种23株革兰阳性球菌可引起皮 肤烫伤综合征病变。表明化妆品中除金黄色葡萄球菌可产生表皮剥脱性毒素外,其它少数凝固酶阴性葡萄球菌(CNS)① 也可产生上述毒素。

Objective To study the action and mechanism of staphylococcal exfoliative toxin A(E-TA)on pemphigus foliaceus antigen(PFA)and pemphigus vulgaris antigen(PVA)expressed on cultured human keratinocytes.Methods Stratified human keratinocytes were incubated with ETA and then stained with sera from patients with pemphigus foliaceus or pemphigus vulgaris as the first antibodies and FITC-la-beled sheep anti-human IgG as the second antibody.Total protein was harvested from the cells pretreated with ETA and run...

Objective To study the action and mechanism of staphylococcal exfoliative toxin A(E-TA)on pemphigus foliaceus antigen(PFA)and pemphigus vulgaris antigen(PVA)expressed on cultured human keratinocytes.Methods Stratified human keratinocytes were incubated with ETA and then stained with sera from patients with pemphigus foliaceus or pemphigus vulgaris as the first antibodies and FITC-la-beled sheep anti-human IgG as the second antibody.Total protein was harvested from the cells pretreated with ETA and run on SDS-PAGE for Western blot with the same antibodies.Simultaneously,supernatants of the keratinocytes before and after ETA treatment were collected for detection of the levels of IL-1α,IL-6with ELISA kits.The caseinolytic activities of the supernatants were tested by spectrometry in which casein was used as a non-specific substrate.Results Down-expression of PFA was shown after ETA treatment while no change of PVA expression was found.The high intensity and continuous linear appearance of fluo-rescent staining before ETA treatment became weak and discontinuous after ETA treatment,which were re-covered gradually in24hours.The degradation of proteins recognized by PF sera after ETV treatment was revealed by Western blot.The decreasing tendency of IL-1αconcentration was found in the supernatants of cell culture after ETA treatment,but IL-6level was too low to be detected.Increased caseinolytic activities were found in the supernatants,and declined36hours after ETA treatment.Conclusions ETA acts on PFA expressed on keratinocytes in vitro,which is reversible along with withdrawal of ETA.The mechanism of E-TA act on PFA may be related to proteolytic action instead of promoting cytokine secretion.

目的探讨表皮剥脱毒素A(ETA)对体外培养正常人角质形成细胞表面天疱疮抗原的影响及其作用机制。方法人角质形成细胞复层化表达落叶型天疱疮抗原后与ETA作用,分别用寻常型或落叶型天疱疮抗体进行免疫荧光染色,提取培养细胞总蛋白做免疫印迹;测定ETA作用后细胞上清液中IL-1α,IL-6的浓度及其分解酪蛋白的活性。结果ETA作用前后细胞间落叶型天疱疮抗体着色由强的连续发光转变为弱的断续发光,24h后恢复,寻常型天疱疮抗体着色无明显变化;免疫印迹检测显示ETA作用后落叶型天疱疮抗原被降解,寻常型天疱疮抗原未受影响。人角质形成细胞与ETA作用后IL-1α分泌受抑制,IL-6的分泌量低于检测水平;ETA作用后细胞上清液分解酪蛋白的活性24h内逐渐增加,36h后活性下降。结论ETA的作用位点在落叶型天疱疮抗原,对寻常型天疱疮抗原无影响;ETA的这种作用具有可逆性,可能通过蛋白酶解作用降解落叶型天疱疮抗原。

Objective To study the correlation between dermatosis and the serotype of exfoliative toxins(ET),and identify the correlativity of the serotype to impetigo or staphylococcal scalded-skin syndrome(SSSS).Methods Sixty strains of Staphylococcal aureus(SA) isolated from patients were studied.Among them,15 strains were isolated from patients with bullous impetigo patients,15 strains from patients with nonbullous impetigo,and 30 patients with strains from SSSS.The reversed passive latex agglutination has been...

Objective To study the correlation between dermatosis and the serotype of exfoliative toxins(ET),and identify the correlativity of the serotype to impetigo or staphylococcal scalded-skin syndrome(SSSS).Methods Sixty strains of Staphylococcal aureus(SA) isolated from patients were studied.Among them,15 strains were isolated from patients with bullous impetigo patients,15 strains from patients with nonbullous impetigo,and 30 patients with strains from SSSS.The reversed passive latex agglutination has been used to detect exfoliative toxin A and B.Results There were 51 exfoliative toxin producers detected by the EXT-RPLA,including 3 serotype A producers and 8 serotype B producers,and 40 double producers.Of the 15 isolates from the skin of patients with bullous impetigo,12 isolates were positive in terms of exfoliative toxin production,including 2 serotype A producers and 10 double producers,with no serotype B producer.Of the 15 isolates from the skin of patients with nonbullous impetigo,10 isolates were positive in terms of exfoliative toxin production,including 1 serotype A producer and 9 double producers,with no serotype B producer.On the other hand,of the thirty SA isolates from patients with SSSS,29 exfoliative toxin producers were detected with the EXT-RPLA,including 8 serotype B producers,21 double producers and with no serotype A producer.Conclusion ETA is correlated with impetigo and ETB is associated with generalized SSSS.ET-producing SA is the most common pathogenic bacteria in these two diseases.

目的探讨脓疱疮和金葡菌烫伤样皮肤综合征(SSSS)两者病原菌分泌表皮剥脱毒素(ET)的情况及与疾病的相关性。方法分离脓疱疮和SSSS患者的病原菌并保存,应用反向乳胶凝集法测定金黄色葡萄球菌(SA)产生ET的型别。结果60株SA中,51株(85%)检测到有ET产生,其中表皮剥脱毒素A(ETA)阳性3株(5%),表皮剥脱毒素B(ETB)阳性8株(13%),ETA和ETB均阳性40株(67%)。30株来源于SSSS的SA,29株(97%)有ET产生;单纯ETB阳性者8株(27%);ETA和ETB均阳性21株(70%)。来源于脓疱疮的30株SA中,22株(73%)有ET产生;单纯ETA阳性3株(10%);ETA和ETB均阳性19株(63%)。其中来源于非大疱型脓疱疮的SA共15株,10株(67%)有ET产生。单纯ETA 1株(7%);ETA和ETB均阳性9株(60%)。来源于大疱型脓疱疮的SA共15株,12株(80%)有ET产生;单纯ETA 2株(13%);ETA和ETB均阳性10株(67%)。结论北京及其周边地区来自儿童脓疱疮和SSSS的SA以产ET菌为主,其中大部分可同时产生ETA和ETB。

 
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