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beat rhythmically
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  搏动
     It was determined the cells' livability,beat rhythmically and beat amplitude of cardiac myocytes were examined. The expression of CP,HP and FP1 was also examined.
     用原代培养的乳鼠心肌细胞为材料,以不同浓度的DFO孵育细胞,然后检测心肌细胞存活率、搏动频率以及铁转运相关蛋白CP,HP,FP1的表达变化.
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  “beat rhythmically”译为未确定词的双语例句
     Compared with SOCS1 group,in dnSOCS1 group the Phospho-STAT1 was more obviously,the myocardocytes beat rhythmically longer.
     另外在dnSOCS1组磷酸化STAT1的表达更明显。
短句来源
     After 18 d,there were 6 cell clusters differentiated into cardiomyocytes which could beat rhythmically even after another continuous culture of 15 d.
     培养 1 8天后 ,有6个集落分化为有节律跳动的心肌细胞 ,这些细胞集落在继续培养 1 5天后 ,仍能有节律收缩。
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  相似匹配句对
     Superfluorescence and its Beat Effect
     超荧光及其拍效应
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     NEW FASHION beat
     新时尚节拍
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     After 18 d,there were 6 cell clusters differentiated into cardiomyocytes which could beat rhythmically even after another continuous culture of 15 d.
     培养 1 8天后 ,有6个集落分化为有节律跳动的心肌细胞 ,这些细胞集落在继续培养 1 5天后 ,仍能有节律收缩。
短句来源
     Compared with SOCS1 group,in dnSOCS1 group the Phospho-STAT1 was more obviously,the myocardocytes beat rhythmically longer.
     另外在dnSOCS1组磷酸化STAT1的表达更明显。
短句来源
     Such zoning is also referred to as rhythmically zoned structure.
     它们亦可以统称为韵律式带状构造。
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  beat rhythmically
Untreated hearts beat rhythmically for up to 9 days (average = 6.8 days); treated hearts stopped beating between 2 and 7 days after treatment.
      
The abilities of the cells to thrive in culture and to beat rhythmically were also used in the comparison.
      
Aggregates continued to beat rhythmically and in a coordinated fashion even after 72 hr in inhibitor.
      
All cells beat rhythmically at 35°C and exhibited normal pacemaker action potentials and If current, regardless of the recording method.
      
The definitive myocardial tube forms in the absence of the endocardial cells and is demarcated into chambers that beat rhythmically and sequentially.
      
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Objective:To culture human embryonic stem cell (EG cell) in vitro and to observe the differentiation of cultures without any cytokines in presence.Methods:Human embryonic (4-6 weeks postfertilization) genital ridges,mesenteries and mesonephros ridge were cultured.Histochemistry and immunohistochemistry were used to identify the cultures.Results:Cultured for 7-8 h,fibroblasts appeared around the tissue fragments.After 5-7 d,EG cluster grew on the fibroblasts and expressed the cell surface markers SSEA-1,SSEA-3...

Objective:To culture human embryonic stem cell (EG cell) in vitro and to observe the differentiation of cultures without any cytokines in presence.Methods:Human embryonic (4-6 weeks postfertilization) genital ridges,mesenteries and mesonephros ridge were cultured.Histochemistry and immunohistochemistry were used to identify the cultures.Results:Cultured for 7-8 h,fibroblasts appeared around the tissue fragments.After 5-7 d,EG cluster grew on the fibroblasts and expressed the cell surface markers SSEA-1,SSEA-3 and alkaline phosphatase (ALP) was positive.After 18 d,there were 6 cell clusters differentiated into cardiomyocytes which could beat rhythmically even after another continuous culture of 15 d.ALP was negative in these cell clusters but there were still a few cells which could remain SSEA-1 and SSEA-3 positive.Conclusion:Culturing human genital ridges,mesenteries and mesonephros ridges in tissue using the endogenous fibroblasts as feeder cell layer,human stem cells (EG cells) clusters can be obtained,and some of the clusters can spontaneously differentiate into cardiomyocytes.

目的 :体外培养并鉴定人胚胎干细胞 (EG细胞 ) ,在不添加细胞因子的条件下 ,观察细胞集落分化情况。方法 :取 4~ 6周人胚胎生殖嵴、肠背系膜和中肾嵴 ,进行组织块培养 ,利用组织化学和免疫组织化学技术对培养的细胞进行鉴定。结果 :培养 7~ 8小时 ,在组织块周围出现成纤维细胞 ;5~ 7天后 ,在成纤维细胞上出现EG细胞集落 ,集落内细胞表达阶段特异性胚胎表面抗原SSEA 1和SSEA 3,并表达碱性磷酸酶 (ALP)活性 ;培养 1 8天后 ,有6个集落分化为有节律跳动的心肌细胞 ,这些细胞集落在继续培养 1 5天后 ,仍能有节律收缩。此时集落内细胞ALP表达呈阴性 ,但仍有少量细胞表达SSEA 1和SSEA 3。结论 :体外培养人胚生殖嵴、肠背系膜和中肾嵴组织块 ,以内源性的成纤维细胞作为饲养层 ,可以分离得到EG细胞集落 ,一些EG细胞集落可以自发地分化成心肌细胞。

Objective To observe the effect of interferon-gamma(IFN-γ) on antiviral activity in rat cardiomyocytes infected with CVB_3 through transfecting the intrinsic JAK/STAT inhibitor, the suppressor of cytokine signaling 1(SOCS1) or the dominant-negative SOCS1(dnSOCS1). Methods The SOCS1 or dnSOCS1 DNA fragment was inserted into a green fluorescent protein fusion vector, which was then transfected into the rat cardiomyocytes with lipofectamine 2000, subsequently, IFN-γ stimulated the cells then CVB_3 was infected....

Objective To observe the effect of interferon-gamma(IFN-γ) on antiviral activity in rat cardiomyocytes infected with CVB_3 through transfecting the intrinsic JAK/STAT inhibitor, the suppressor of cytokine signaling 1(SOCS1) or the dominant-negative SOCS1(dnSOCS1). Methods The SOCS1 or dnSOCS1 DNA fragment was inserted into a green fluorescent protein fusion vector, which was then transfected into the rat cardiomyocytes with lipofectamine 2000, subsequently, IFN-γ stimulated the cells then CVB_3 was infected. The expression of SOCS1 and dnSOCS1 was observed by the fluorescent microscope. Western blot was used to study the protein of Phospho -STAT1 in different groups.Results Virus titer was higher in the SOCS1 transfected group than in dnSOCS1 group( P <0.05). Compared with SOCS1 group,in dnSOCS1 group the Phospho-STAT1 was more obviously,the myocardocytes beat rhythmically longer.Conclusions dnSOCS1 could enhance the antiviral activities of IFN-γ in vitro. SOCS1 may be the new therapeutic target in the viral myocarditis.

目的病毒性心肌炎(VM)细胞模型JAK/STAT1信号通路的特异性内源抑制物细胞因子信号转导抑制物-1(SOCS1)及其负显突变体SOCS1(dnSOCS1)对IFN-γ抗CVB3活性影响。方法构建pEGFP-C1-SOCS1及pEGFP-C1-dnSOCS1的真核表达载体,以脂质体法转染至心肌细胞后,加用IFN-γ刺激细胞并感染病毒,采用荧光显微镜检测心肌细胞中SOCS1和dnSOCS1表达,同时利用Westernblot法测定不同条件下STAT1表达水平并测定病毒滴度。结果与转染dnSOCS1组比较,转染SOCS1组病毒滴度高,而心肌细胞存活率低,心肌细胞搏动时间短。另外在dnSOCS1组磷酸化STAT1的表达更明显。结论dnSOCS1可增强IFN-γ体外抗病毒活性。SOCS1可为我们治疗VM提供一个新的治疗靶点。

To observe the effect of DFO on iron metabolism in primary cultured rats' cardiac myocytes.Newborn rat cardiac myocytes were isolated and cultured with the medium containing DFO.It was determined the cells' livability,beat rhythmically and beat amplitude of cardiac myocytes were examined.The expression of CP,HP and FP1 was also examined.Results showed that compared with control,DFO had no clearly influence on cells' livability,but it could decrease cells' beat rhythmically and beat amplitude.CP...

To observe the effect of DFO on iron metabolism in primary cultured rats' cardiac myocytes.Newborn rat cardiac myocytes were isolated and cultured with the medium containing DFO.It was determined the cells' livability,beat rhythmically and beat amplitude of cardiac myocytes were examined.The expression of CP,HP and FP1 was also examined.Results showed that compared with control,DFO had no clearly influence on cells' livability,but it could decrease cells' beat rhythmically and beat amplitude.CP and HP expression increased after the incubation of DFO,however,FP1 expression decreased.DFO has no influence on cardiac myocytes' livability,but it could affect the cells' living state.DFO could regulate and control expression of iron transport proteins.

观察去铁胺(DFO)对原代培养的心肌细胞铁代谢的影响,探讨DFO对心肌细胞铁代谢的影响机制.用原代培养的乳鼠心肌细胞为材料,以不同浓度的DFO孵育细胞,然后检测心肌细胞存活率、搏动频率以及铁转运相关蛋白CP,HP,FP1的表达变化.结果表明:各剂量DFO对心肌细胞存活率无明显影响;心肌细胞搏动频率减慢,停止跳动的细胞数量明显增加,收缩幅度逐渐降低;随着DFO浓度的增加,心肌细胞CP和HP的表达增加而FP1表达减少.由此可以看出,DFO不影响心肌细胞的存活率,但影响细胞的生活状态,也影响心肌细胞内CP,HP和FP1蛋白的表达.

 
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