By the fermentation in shake flask,it was studied that the influence of temperature,the shaking speed of shake flask,the kind and concentration of carbon source and the kind and concentration of nitrogenous source on the production of dicarboxylic acid by fermentation of Candida tropicalis.
Compared with shaking flask experiment,the MBR system effectively prevented the product inhibition of the enzyme and vanillin oxidation loss,and the bioconversion rate of clove oil increased to 1.04% from 0.45%. The maximum vanillin concentration in receiving solution was 130.10 mg/L which was much higher than 11.13 mg/L in the shaking flask experiment.
Influences of carbon and nitrogen sources on Pseudomonas putida S1 dry weight and on the activity of intracellular trehalose synthase,were studied in shaking flask scaled Fermentation.
Under the optimal fermentation conditions,the activity of fibrinolysin reached 3643 U/ml(urokinase unit) in shake flask,and 2050 U/ml(urokinase unit) in 15 L fermentor.
the optimal fermentation condition were obtained as follow: initial pH value 7.5,temperauture 30℃,fermentation time 96h,seeding volume 7%,rotated speed 180r/min,75 ml medium in 500ml flask.
The optimal medium composition for the lipase production is(%):soybean flour 1.0,corn milk 1.0,glucose 1.0, K2HPO4 0.5,NaNO3 0.5. Culture medium volumes for 50 mL flask were cultivated aerobically at 20℃ with an aeration rate of 180 r/min for 40 hours.
The optimal culture conditions to get cellulose were as follows: cellulose powder 3%,KNO3 3%,PEG 0.1%,initial pH6.5,25 mL medium in 250 mL flask,inoculating 1 day of seeds 10%,32 ℃ culture it for 5 days.
For the sake of contrast, cells were also expanded in a T-flask using a hollow-fiber membrane as carrier and in a rotating wall vessel bioreactor (RWVB) using a microcarrier.
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Several sets of shaking flask experiments were conducted.
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meliloti and application of nitrates 2 weeks after inoculation was optimal for active nitrogen fixation (224.7 C2H4 nmol/flask · 24 h) and low denitrification activity (1.8 μmol N2O/flask · 24 h).
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An endophyte (designated ZP5SE) was isolated from the seed of Nothapodytes foetida and was examined as a potential source of anticancer drug lead compound, i.e., camptothecin, when grown in Sabouraud liquid culture media under shake flask conditions.
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The stiff structure of the microscope and its small transverse dimensions allow scanning to be performed in the atomic-force-microscope mode of a portable Dewar flask with a neck diameter of >amp;gt;40 mm without using additional vibration insulation.
This paper describes a quick and easy qualitative test and the paper chromatographic qualitative test of malicci cid. The experimental processes and methods of the direct fermentation of L-malic Acid by Aspergillus Species will be Summarized. The ASP. Weetii, ASP. Niger, ASP. flavus. and ASP. oryzae have been isolated from Soil. The C~6_0~0-ray, 5-FU and high Temperatuve have been used respectivly or combinedly To treat Some of the Species in the experiment. We have obtained some mutants from the wild type ...
This paper describes a quick and easy qualitative test and the paper chro- matographic qualitative test of malicci cid. The experimental processes and methods of the direct fermentation of L-malie Acid by Aspergillus Species will be Summarized. The ASP. Weetii, ASP. Niger, ASP. flavus, and ASP. oryzae have been isolated from Soil. The C_0~(60)-ray, 5-FU and high Temper- atuve have been used respectivly or combinedly To treat Some of the Species in the experiment. We have obtained some mutants from the wild ...
Bacillus subtilis 478 selected for the unhairing of hide is a strain producing Neutral protease.The slant colony inoculated into 50 ml of medium in a 500 ml of shaking-flask,cultiveted for 40 h.At 31℃ and produced neutral protease 5502 u/ml.The strain cultivated in a modal BF-12-1 fermentor for 28 h.At 31℃ produced Neutral protease 5795 u/ml.The optimum PH for proteolytic activity on casein was found to be 7.0.The enzyme was stable and more active at PH 6.5—7.5.The proteolytic activity decreased by 12...
枯草芽孢杆菌478是为生皮脱毛选育的一株中性蛋白酶产生茵。500 ml 摇瓶,装量50 ml 培养基,接种一小铲,31℃摇床培养40小时,产酶为5502 u/ml。BF—12—Ⅰ型罐发酵28小时,31℃产酶为5795 u/ml。酶的酪蛋白水解最适 PH 为7.0,活性范围为 PH 6.5～7.5,此间热稳定性较好,40℃两小时失活为12%。酪蛋白水解的最适温度为40～50℃。Fe ~(++)Hg~(++)与 Cu 对酶有抑制作用,Ca~(++)与 Mg~(++)则有激活作用。聚丙烯酰胺凝胶电泳分析得到9条带。国内外一直沿用的硫化碱脱毛工艺对环境污染严重。酶法脱毛则是解决污染问题的可行途径。半个世纪以来人们对此作了积极的研究。然而,可用的产酶株仍是屈指可数,脱毛工艺的革新受到很大限制。为了推进酶法脱毛工艺向前发展,我们选育了脱毛效果较好的枯草芽孢杆菌478中性蛋白酶产生株。本文对该茵的发酵及其产生的中性蛋白酶的一些基木特性作一报导。
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