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radiochemical
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  radiochemical
Functional activity of blood cells in fish exposed to radiochemical industrial sewage
      
The effect of the gas components of the working medium, which develops during the reprocessing of waste nuclear fuel at radiochemical plants, on the 129I fluorescence excited by radiation of He-Ne laser (633 nm) is studied.
      
The results were compared to the data obtained using radiochemical separation.
      
This instrument can measure 241Am activity in samples, without their concentration and radiochemical treatment, against a high (~10-3) background level due to the accompanying radionuclides (134Cs, 137Cs, 154Eu, 155Eu, etc).
      
A Radiochemical Installation for Handling Small Amounts of High-Purity Gases
      
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The t-butylisonitrile compound is synthesized. The cationic complex Tc- 99m hexakis (t-butylisonitrile) technetium (TBI) is labelled by kit method. The reaction is accomplished in boilling water for 1 min(or at 80℃ for 5 min). The labelling efficiency and radiochemical purity of ~(99m)T-TBI are both more than 98%. The animal studies indicate that ~(99m)Tc-TBI is an excellent myocar- dial perfusion imaging agent. It has had succese in human bodies as well.

本文在国内首次报导用自己合成的特丁基异腈制备成功~(99m)Tc-TBI标记物.采用药盒标记法,沸水浴中反应1min(或80℃,反应5min)即可完成.鉴定表明,~(99m)Tc-TBI的标记率及放化纯度均在98%以上,产物无需分离,即可直接用于静脉注射.通过对小白鼠、家兔的研究表明,该标记物在正常心肌中有较高的浓集和较长的保留时问,同时具有相当快的血清除率,是良好的心肌灌注显像剂.该标记物用于国内临床研究亦获成功.

The carbomethoxyisopropyl isonitrile (CPI) is synthesized. The cationic complex ~(99m)Tc-CPI is labelled by kit method. The reaction is accomplished in bolling water for 5~10 min. The labelling efficiency and radiochemical purity of ~(99m)Tc-CPI are both more than 95%. The animal studies indicate that ~(99m)Tc -CPI has high myocardial uptake and rapid lung and liver clearance in dog and rabbit.

采用以α-氨基异丁酸为起始物制备甲酯异丙异腈的化学合成路线,并用自己合成的产物成功地制备了放射性~(99m)Tc-CPI标记化合物.通过聚酰胺薄片层析鉴定表明,~(99m)Tc-CPI的标记率及放化纯均在95%以上,产物无须分离,即可直接用于静脉注射.

The preparation of 125I(131I)-anti-CEA monoclonal antibody by using several radio-iodination methods (Iodogen, ChT, NBS, and Bolton-Hunter reagent etc.) was described. Purification of the product was carried out by the Sephadex G-50 (or 75) gel-filtration column. Its radiochemical purity checked by paper chromatography and HPLC was satisfactory for use. The labelled antibody showed good immunoreactivity, when it was measured by IRMA-bi-antibody sandwich method. The comparative studies for above several...

The preparation of 125I(131I)-anti-CEA monoclonal antibody by using several radio-iodination methods (Iodogen, ChT, NBS, and Bolton-Hunter reagent etc.) was described. Purification of the product was carried out by the Sephadex G-50 (or 75) gel-filtration column. Its radiochemical purity checked by paper chromatography and HPLC was satisfactory for use. The labelled antibody showed good immunoreactivity, when it was measured by IRMA-bi-antibody sandwich method. The comparative studies for above several radioiodination methods were carried out, among which the iodination efficiency of NBS method was the best (98%) while the immunoreactivity of lodogen method was the best and specific binding of labelled antibody to CEA was 9960 cpm (3ng CEA/ 250 μl).

本文报道用几种碘标记方法(Iodosen、ChT、NBS、Bolton-Hunter试剂等)制备了~(125)I(~(131)I)-抗CEA单克隆抗体。产品通过Sephacex G-50(或-75)柱凝胶过滤纯化后,经纸层析和高压液相层析法鉴定,其放化纯度达到使用要求;用免疫放射双抗体夹心法测定,标记抗体能保持良好的免疫活性。同时我们对上述几种碘标方法进行比较研究,结果NBS法标记率最高,达98%,Iodogen法免疫活性保持最好,与CEA的净结合数达9960cpm(3ng CEA/250μl)。

 
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