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infective dose
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  感染量
     Methods RT-PCR assay was used to detect the mRNA expression of CMV immediate early gene(IE),glyceraldehyde phosphate dehydrogenase(GAPDH) and IL-6 of KM3 cells infected by 100-,10-,1-folds of median tissue culture infective dose(TCID_(50)) of CMV. Flow cytometry was used to detect the expression of pp65 antigen. CMV particles were detected with electron microscope.
     方法 以 10 0 ,10 ,1半数组织培养感染量 (TCID50 )滴度的CMV与KM3细胞共培养 ,RT PCR法检测细胞CMV即刻早期抗原基因 (IE)、甘油醛 3 磷酸脱氢酶基因 (GAPDH)及IL 6mRNA的表达 ,流式细胞术检测细胞CMVpp6 5抗原的表达 ,透射电镜检测细胞内CMV病毒颗粒。
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     After ten times continuous dilution,chicken embryo fibroblast (CEF) and chicken embryo were inoculated with pigeon Paramyxovirus Ⅰstrain JS for determining its median tissue culture infective dose(TCID 50 )and 50% egg infectious dose(EID 50 ).
     鸽I型副粘病毒 (PPMV Ⅰ )JS株的尿囊液毒 ,经 1 0倍连续稀释后分别接种鸡成纤维细胞和非免疫鸡胚 ,测定其半数细胞培养感染量 (TCID50 )和鸡胚半数感染量 (EID50 )。
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     Influence of Different Infective Dose of REV on Immunity Responses and Cytotoxic Activity in Chickens
     不同感染量REV对鸡免疫反应和细胞毒性作用的影响
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     Methods The influences of culture liquid, passage, cell age and infective dose on the liter and yield of poliovirus were studied.
     方法 检测不同培养液、不同代次、不同细胞龄和不同病毒感染量等因素对病毒滴度及产量的影响。
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     w illmattianum in various concentration was applied to different steps of HSV -1 replication cycle. 50% Tissue culture infective dose (TCID 50 ), cytopat hic effect(CPE), MTT staining method, dot blotting and Northern blotting anal ysis were used to estimate index of antiviral activity.
     方法 :采用不同剂量的紫金标作用于适量HSV 1感染的Vero细胞 ,以 5 0 %组织细胞感染量 (TCID50 ) ,细胞病变效应(CPE) ,MTT法和核酸分子杂交作为评价指标。
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  感染剂量
     After NDV were inoculated for 72 h,the 50% tissue culture infective dose(TCID 50 )of NDV Ⅰ strain and hemagglutination titer of NDV Ⅳ strain were determined to evaluate the effects of the three components on cellular infectivity of the virus.
     于病毒接种后 72h测定NDVⅠ系的半数组织培养感染剂量 (TCID50 )和NDVⅣ系的血凝效价 ,以评价 3种中药成分对NDV感染细胞的影响。
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     When infective dose ranged from 0 5 to 3 0×10 4 oocysts per chicken,level of NO 2 increased in a stepwise manner with increasing infective dose.
     在 0 5× 10 4 ~ 3 0× 10 4 个 /只的感染范围内 ,实验鸡血浆亚硝酸盐水平随着感染剂量的增加而升高。
短句来源
     However, the activity was decreased when infective dose was equal to or higher than 3.0×104 oocysts per chicken.
     但感染剂量在等于或高于3.0×104 个卵囊/只时,活性开始下降。
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     The recombinant adenovirus titers were tested with median tissue culture infective dose (TCID50) method.
     B.B DNA。 转染并荧光显微镜观察eGFP的表达,进行重组腺病毒的PCR鉴定,重组腺病毒扩增后氯化铯密度梯度离心法纯化Ad-VEGF_(165),TCID_(50) 50%组织培养感染剂量法,测定腺病毒滴度。
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     Methods HIV was exposed to different concentrations of ascorbic acid at different temperature. The inactivition result of ascorbic acid on HIV was explored by detecting median tissue culture infective dose (TCID50) at different intervals. The cyto-toxicity of the effective dose on MT4 cell was also explored.
     方法通过测半数组织培养感染剂量(TCID50),检测不同浓度维生素C在不同温度和作用时间下对HIV的灭活作用,并对有效剂量组进行MT4细胞毒性试验。
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  “infective dose”译为未确定词的双语例句
     The results showed that the potency was 5.2、(3.6)、2.1、1.0 PD_(50) per dose when using 500、1 000、2 000、4 000 ID_(50)which is the median infective dose as the challenge viral dosage, respectively.
     结果表明 ,分别用 5 0 0ID50 、10 0 0ID50 、2 0 0 0ID50 和 4 0 0 0ID50 的病毒进行攻毒 ,疫苗每头份分别含 5 .2、3.6、2 .1和 1.0个PD50 。
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     The Szg and Ssq strains had clear cytopathic effect(CPE) on Vero cell, and the 50% tissue culture infective dose were TCID_(50)=10~(-3.925)/0.5ml, TCID_(50)=10~(-3.223)/0.5ml, respectively. Spherical and capsular virus particles were observed in electron microscope in Vero-cultured virus.
     Ssq株和Szg株在Vero细胞上的细胞病变(CPE)明显,对细胞的感染毒力分别为TCID_(50)=10~(-3.925)/0.5ml和TCID_(50)=10~(-3.223)/0.5ml。
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     The infective dose of 50% tissue culture (TCID50) to H. K. cells was up to 107/0.1ml, that of embryo 108/0.1ml and that of allantoic fluid 102/0.1ml.
     K细胞半数感染量(TCID_(50))达10~7/0.1ml,胚胎为10~6/0.1ml,尿囊液为10~2/0.1ml;
短句来源
     Examine the 50% embryo lethal dose (ELD50) of the strain GX8/99 and the cell-cloned virus separately, and examine the mean tissue culture infective dose (TCID50 ) of the cell-cloned virus .
     对超强毒IBDV GX8/99株原始毒囊毒及其克隆化细胞毒进行ELD50测定,并对克隆化细胞毒进行TCID50测定。
短句来源
     The rescued virus R- A/Chicken/Guangdong/04(R-CG) and the wild type A/Chicken/Guangdong/04(W-CG) shared similar biological properties such as in titers of 50% embryo infective dose(EID50), 50% tissue culture infective dose(TCID50) and intravenous pathogenecity index(IPVI).
     实验通过反向遗传操作技术对该病毒进行拯救,获得了拯救毒R-A/Chicken/Guangdong/04(R-CG)。 R-CG与其亲本毒A/Chicken/Guangdong/04(W-CG)在胚半数感染量(EID50)、细胞培养半数感染量(TCID50)、对SPF鸡和BALB/c小鼠致病力等生物学特性保持一致。
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  infective dose
Maximum virus yields in MARC-145, P, and L-1 cell clones were 108.5, 103.5, and 102.5 tissue culture infective dose 50 (TCID50)/0.1 ml, respectively.
      
Minimal infective dose of the OSU strain of porcine rotavirus
      
C57BL/6 mice immunized intraperitoneally (i.p.) with an infective dose of JEV were resistant to intracerebral (i.c.) challenge with JEV, whereas most C3H/He mice treated in the same manner died.
      
We studied the minimal infective dose of the gastroenteritis virus, rotavirus.
      
Independent of infective dose within the range examined, the virulent isolate caused fatal clinical disease, whereas the avirulent isolate caused subclinical infection.
      
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1. By cultivating the lapinized hog cholera vaccine virus(LHCV) on pig kidney cells in rolling bottles with increased volume of cultural media(3—4 times more than the amount used in the routine method), the titres in the first and second harvests of vaccine virus were quite similar to those obtained by the routine method. The titres in the second harvest passed most of the tests of 5×10~4 RID/1ml (Rabbit Infective Doses). Results of the tests met entirely the demand for the mass production of vaccine....

1. By cultivating the lapinized hog cholera vaccine virus(LHCV) on pig kidney cells in rolling bottles with increased volume of cultural media(3—4 times more than the amount used in the routine method), the titres in the first and second harvests of vaccine virus were quite similar to those obtained by the routine method. The titres in the second harvest passed most of the tests of 5×10~4 RID/1ml (Rabbit Infective Doses). Results of the tests met entirely the demand for the mass production of vaccine. 2. The method of cultivating hog cholera vaccine virus in rolling bottles around an inclined axis and with deeper culture media(7—8 times more than in the routine method) produced titres in the first and second harvests or in a single harvest(7 days after inoculation) quite similar to those obtained by the routine method.They fitted well the requirements for vaccine production. 3. We also cultivated the LCHV in rolling bottles as cited above but with even much more culture media(8 times more) and additional aerati on. In this case the titres obtained in single harvests(7 days after inoculation)passed most of the 5×10~4RID/1 ml tests also. The data given above suggested that these three methods of cultivation might be applicable in actual vaccine production, with the only consideration that much greater amounts of culture media than used in the routine method of production had to be consumed in these cases. These modifications would much simplify the production of LCHV vaccine.

1、用增加培养液量(3—4倍)旋转法培养猪瘟弱毒细胞疫苗,第一收、第二收病毒收获液的毒价均可达到普通旋转培养法的滴度。二收的绝大多数批次毒价用兔检5万倍通过,符合制苗要求。 2、用深液(培养液量增加7—8倍)倾斜旋转法培养猪瘟弱毒细胞疫苗。第一收与第二收的毒液或培养7天一次全收的毒液,其大多数试验批次毒价均可达到制苗标准。 3、用深液(培养液量增加8倍)倾斜旋转通气法培养猪瘟弱毒细胞疫苗,接毒后培养7天一次全收毒液,大多数批次试验毒价用兔检5万倍通过。符合制苗要求。以上三种培养方法所得资料说明提高猪肾细胞产毒量是明显的,工艺是简便的,在生产上是可行的。对细胞提高繁殖病毒能力的问题进行了某些探索。

Pseudorabies virus can infect chick embryo and reproduce there by way of passing through egg yolk, allantoic cavity, allanto chorion. After infection, various gross lesions appear. The chick embryo inoculated through egg yolk sac and allantoic carity dies in 48-72 hours. In our experiments we found that the toxicity of amniotic fluid (a. f.) of the infected embryo through egg yolk was the highest. The infective dose of 50% tissue culture (TCID50) to H. K. cells was up to 107/0.1ml, that of embryo 108/0.1ml...

Pseudorabies virus can infect chick embryo and reproduce there by way of passing through egg yolk, allantoic cavity, allanto chorion. After infection, various gross lesions appear. The chick embryo inoculated through egg yolk sac and allantoic carity dies in 48-72 hours. In our experiments we found that the toxicity of amniotic fluid (a. f.) of the infected embryo through egg yolk was the highest. The infective dose of 50% tissue culture (TCID50) to H. K. cells was up to 107/0.1ml, that of embryo 108/0.1ml and that of allantoic fluid 102/0.1ml. To the inoculated allantoic cavity,the TCID60 of allantoic and amniotic fluid was 105/0.1ml, and that of embryo was 103/0.1ml.The pathological .changes occured in fifteen different monolayer cells of H.K., M.K, etc and were mainly two types: one was a big fusion or atr-ophic round necrosis in monolayer kidney cells and the other was atrophic round necrois in the cells of chick embryo.After the growth and reproduction of the virus in H.K. cells, the virus titre in the culture fluid, cytoplasm and cell karyoplasm was estimated 108 TCID50/0.1ml.when the virus reproduces continuously from generation to generation, its titre was up to 108 TCID60/0.1ml. The time of the appearence of pathological changes of cells was only 3 hours in comparison of 24 hours before.

牛伪狂犬病病毒(闽A株)能通过鸡胚卵黄囊、尿囊腔、尿囊膜感染和繁殖,并出现不同程度的病变,经卵黄囊、尿囊腔接种的鸡胚,均于48~72小时死亡,死亡率达100%。经卵黄囊接种的鸡胚其羊水含毒量最高,对H.K细胞半数感染量(TCID_(50))达10~7/0.1ml,胚胎为10~6/0.1ml,尿囊液为10~2/0.1ml;经尿囊腔接种的鸡胚,其尿囊液及羊水均为10~5/0.1ml,胚胎为10~3/0.1ml。 该毒株对H.K、M.K等15种单层细胞均可引起细胞致病作用,其病变可分为二种类型:于单层肾细胞上形成聚融合和萎缩圆形坏死,在鸡胚等细胞上形成萎缩圆形坏死。 病毒在H.K细胞上生长繁殖后,经测定在上清液、细胞质、细胞核质中均含有10~6TCID50/0.1ml的毒价。 牛伪狂犬病病毒经H.K细胞连续传代后,对H.K细胞的毒力可达10~8TCID_(50)/0.1ml,出现细胞病变的时间由原来的24小时缩短为3小时。

Growth conditions of EHF virus in primary rat lung cell (RLC) culture were studied. The preliminary results were as follows : (l) different virus strains at various infective doses could multiply to relatively high tilers in 6-10 days after infection; (2) multiplication of EHF virus was much better at 33℃ than that at 37℃ and if EHF virus was first cultivated at 37℃, and then transferred to 33℃, better multiplication might be obtained, (3) the tilers of EHF virus in the maintainance medium of infected...

Growth conditions of EHF virus in primary rat lung cell (RLC) culture were studied. The preliminary results were as follows : (l) different virus strains at various infective doses could multiply to relatively high tilers in 6-10 days after infection; (2) multiplication of EHF virus was much better at 33℃ than that at 37℃ and if EHF virus was first cultivated at 37℃, and then transferred to 33℃, better multiplication might be obtained, (3) the tilers of EHF virus in the maintainance medium of infected cell cultures were similar whether the maintainance medium contained calf serum or not, (4) treatment of the whole cell culture with repeated freezing and thawing failed to increase the virus yields. In primary RIC culture system, conditions used in these studies could not give the expected high titer desired for vacine produchtion and further studies for the optimal growth conditions are still needed.

EHFV在RLC中,不同毒株及感染剂量均能在感染后6~10天达繁殖高峰;33℃比37℃培养好;先在37℃后转33℃培养利于提高病毒产量;维持液中有否小牛血清对病毒繁殖无明显影响;冻融对提高病毒液度无好处;在RLC系统中EHFV 繁殖未达满意高滴度。

 
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