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ribosome
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  核糖体
     In 58 cases of SLE the positive rates of Sm,RNP,SSA,SSB and ribosome antibodies were 36.21%,34.48%,22.41%. 8.62%and 17.24% tespectively.
     58例SLE患者Sm抗体为36.21%,RNP抗体为34.48%,SSA抗体为22.41%,SSB抗体为8.62%,核糖体抗体为17.24%。
短句来源
     Roles of Ribosome S6 Protein Kinase p90~(rsk) in Oocyte Meiosis
     核糖体S6蛋白激酶p90~(rsk)与卵母细胞减数分裂
短句来源
     Real-time Fluorescence Quantitative PCR in Detecting Ribosome Protein S13(RPS13) Gene Expression in NK/T Cell Lymphoma
     实时荧光定量PCR检测核糖体蛋白S13基因在NK/T细胞淋巴瘤中的表达
短句来源
     After cut down by restriction enzyme,the dhaT gene with own ribosome binding site was inserted into recombining plasmid pMD19-T Simple/gldABC in tandem thereafter,The gene of gldABC and dhaT was cut down from pMD19-T Simple/gldABCdhaT,subcloned into expression vector pET28a(+),and then identified by sequencing and restriction enzyme of XbaI and EcoRI.
     将含有自身核糖体结合位点的dhaT基因片段插入到pMD19-T Simple/gldABC质粒的gldABC基因下游,形成重组克隆质粒pMD19-T Simple/gldABC-dhaT,并进一步将gldABC与dhaT串联基因亚克隆到表达载体pET28a(+)上。
短句来源
     Cloning and Sequencing of the Gene for Cotton Chloroplast 70 S Ribosome S7 Protein
     棉花叶绿体70S核糖体S7蛋白基因的克隆和序列分析
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  核蛋白体
     Expression of Subunits,p40 and p35, of Interleukin 12 Mediated by Internal Ribosome Entry Site of Encephalomyocarditis Virus in B16F1 Cells
     脑心肌炎病毒内部核蛋白体进入位点介导白介素-12基因在B16F1细胞中的表达
短句来源
     To investigate the transcription activity of ribosome deoxyribonucleic acid (rDNA) in peripheral blood T lymphocyte for the diagnosis of digestive system malignant tumor.
     [目的]观察外周血T淋巴细胞脱氧核糖核酸核蛋白体(rDNA)转录活性对消化系统恶性肿瘤的诊断有无临床价值 ,探讨其变化规律。
短句来源
     Cytopathological study showed that GSSD attenuated CCl4 damage of heterochromatin, mitochondrion, endoplasimic reticulum and ribosome.
     超微结构观察证实GSSD能减轻CCl4对肝细胞染色质,线粒体,内质网和核蛋白体的损害。
短句来源
     Whereas in CP+CPABM groups,the tumor cells shrunken with obvious pyknosis,the surface microvillus disappeared,free ribosome reduced notably in cells and mitochondrion in cytoplasm presented vacuolation.
     姬松茸复合多糖各剂量+环磷酰胺组可见肿瘤细胞体积变小,细胞核固缩明显,细胞表面微绒毛消失,细胞内游离核蛋白体明显减少,胞质内线粒体空泡变性。
短句来源
     Electron microscopic examination revealed that mitochondria swelled obviously, the ridge disappeared, ribosome was disarranged and endoplasmic reticulum was expanded and vacuolated in group C and E;
     (2)肝组织超微结构示C组与E组线粒体肿胀,嵴消失或紊乱,核蛋白体脱落,内质网呈空泡状。
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  核糖核蛋白体
     The numbers of mitochondria,plastid and ribosome show an increase in some epidermal cells which kept an ability of active division and may be differentiated into a primordial bud.
     处于旺盛分裂状态有可能分化成芽原基的某些愈伤组织表层细胞中的线粒体、质体、核糖核蛋白体的数量增多了。
短句来源
     The vacuoles increased and free ribosome decreased with the increase of the dose.
     胞质内空泡众多,并随着剂量的增加而明显增多变大; 胞质内游离核糖核蛋白体明显减少。
短句来源
  “ribosome”译为未确定词的双语例句
     ALTERATIONS IN THE PROTEIN SYNTHESIS AND CONTENTS OF RIBOSOME AND POLYSOME IN LIVEROF SELENIUM-DEFICIENT RATS
     ALTERATIONS IN THE PROTEIN SYNTHESIS AND CONTENTS OF RIBOSOME AND POLYSOME IN LIVEROF SELENIUM-DEFICIENT RATS
短句来源
     Active site of trichosanthin acting as a ribosome inactivating protein
     Active site of trichosanthin acting as a ribosome-inactivating protein
短句来源
     (3) Some of the cells showed apoptotic changes in the CP alone group, whereas the intranuclear chromoplasma was well-distributed, the nuclear membrane was clear, and mitochondria, ribosome and solvent were present in the ATRA (10 nmol/ L) + BDNF (50 ng/ml) + CP group.
     (3)透射电镜技术观察到CP作用组可见到较多细胞呈凋亡改变,而ATRA 10 nmol/L+BDNF 50 ng/ml+CP 5μg/ml组细胞形态多数正常。
短句来源
     Analysis of Intergenic Spacer 2 Diversity of Ribosome DNA for Strains of Pleurotus eryngii
     刺芹侧耳(Pleurotus eryngii)rDNA的IGS2多样性分析
短句来源
     Expression of D-Amino Acid Oxidase Gene and Green Fluorescence Protein Gene Transferred into K562e Cells by Retroviral Vector Containing Internal Ribosome Entry Site Sequence
     IRES序列连接的D-氨基酸氧化酶与绿色荧光蛋白基因导入K562e细胞的表达
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  ribosome
The polypeptide chains enter the folding sites from protein translocation complexes (ribosome, translocation machinery incorporated in membranes) directionally with the N-terminus and gradually.
      
Location of Template on the Human Ribosome as Revealed from Data on Cross-Linking with Reactive mRNA Analogs
      
In this review we summarize data on the location of template on the human ribosome that we obtained from cross-linking (affinity labeling) experiments using reactive mRNA analogs.
      
From analysis of the cross-linking data, we suggest a scheme for the arrangement of mRNA on the human ribosome and compare the organization of the mRNA binding center on human and Escherichia coli ribosomes.
      
Trichosanthin (TCS) is a type I ribosome-inactivating protein (RIP) that can selectively kill some types of cells at low concentration (0.1-1 nM).
      
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The cells of the apical ectodermal thickening, neighboring ectoderm and underlyingmesenchyme of the limb buds of mouse embryos, 11--13 days vaginal plug age, wereused for this study. Most of the specimens were fixed in 1% osmic acid in veronalacetate buffer, pH 7.6, 4℃, 1 hour and embedded in methylchrylate. Parts of the speci-mens were either fixed in potassium permagnate or stained in lead hydroxide after osmicacid fixation. Observations and photographs were made under SEM Ⅲ electron micros-cope. The Golgi...

The cells of the apical ectodermal thickening, neighboring ectoderm and underlyingmesenchyme of the limb buds of mouse embryos, 11--13 days vaginal plug age, wereused for this study. Most of the specimens were fixed in 1% osmic acid in veronalacetate buffer, pH 7.6, 4℃, 1 hour and embedded in methylchrylate. Parts of the speci-mens were either fixed in potassium permagnate or stained in lead hydroxide after osmicacid fixation. Observations and photographs were made under SEM Ⅲ electron micros-cope. The Golgi apparatus appeared to be horse-shoe shaped in the apical ectodermalthickening and S-shaped in the neighboring ectoderm. It consisted of small vesicles andtubules without any large vesicle. The mitochondria in the neighboring ectoderm were usually round or oval in shape,with fewer and irregular cristae and lighter matrix. The mitochondria of the mesenchy-mal cells were even more embryonic in structure with only 2--3 oblique cristae and alarge inner chamber. The mitochondria in the supperficial layers of the apical ectoder-mal thickening were more or less similar to those in the neighboring ectoderm. Rod-shaped and filamentous mitochondria increased in number in the deeper layers of theapical thickening. Such filamentous mitochondria had vertical cristae, denser matrix andoval and light inner chambers. Transitional stages between the very small vesicles withvague cristae and the large mitochondria with distinct cristae were found in our leadstained preparations. Except in the Golgi region, ribosomes grouped into ring or tubular forms weredistributed all over the cytoplasm in the three kinds of the embryonic cells studied.Transitional stages between such ribosome clusters and the granulated vesicles and tubulescould be clearly demonstrated in the lead stained specimens. In the apical ectodermal thickening, granulated endoplasmic reticulum in the formof scattered small vesicles and tubules seemed to be more abundant than in the neighbor-ing ectoderm and underlying mesenchymal cells. But the most striking difference foundin the apical thickening was the expansion of the granulated endoplasmic reticula intolarge saccules, the cisternae of which containing a grayish dense substance. Another interesting phenomenon found in the apical ectodermal thickening was thepresence of dense bodies in the cytoplasm. They were in various sizes and densitieswith dense granules, masses or cords and various forms of vesicles. They might be round,oval or irregular with a complete, partial or entire absence of limiting membrane. Thosewithout membrane were usually irregular in form and could hardly be demarkated from their surrounding cytoplasm whence assembly of ribosome clusters, mitochondria andendoplasmic reticulum from the surrounding cytoplasm to form the dense bodies could befound. Preliminary histochemical studies found them to be positive in alkaline and acid-phosphatases and RNA staining. They were therefore considered to be lysosomes orcytosomes. The significance of the differences in mitochondrial form and structure, abundantand expanded cisternae of the granulated endoplasmic reticulum and the presence of thedense bodies in the apical ectodermal thickening in relation to embryonic differentiationof the limb was discussed.

用小白鼠胚胎阴栓日龄11—13天的前肢芽和后肢芽,锇酸固定,甲基丙烯酸甲酯及甲基丙烯酸丁酯包埋,在SEM Ⅲ型电子显微镜观察了肢芽尖端增厚外胚层、邻近普通外胚层及间充质细胞。部分材料用过锰酸钾固定或锇酸固定后氢氧化铅染色。各种细胞相互比较的结果如下: 1.两种外胚层的高尔基体极相似,均由小泡及小管组成,无大泡,横切时成为成群的小泡。普通外胚层细胞的高尔基体呈S形,增厚外胚层的马蹄形。 2.线粒体在普通外胚层卵圆形或圆形,嵴不整齐。增厚外胚层的线粒体在浅层细胞少,结构与普通外胚层相似,但愈至深层则细长的线粒体愈多。细长线粒体的嵴较密,亦较整齐,与表面垂直,基貭亦较致密。在尖端增厚外层细胞见到由胞质新形成的小泡过渡到小圆的线粒体。 3.无论在普通外胚层下或在尖端增厚外胚层下的间充质细胞,其线粒体的形状和结构相同,都是圆或卵圆,嵴少而靠近表面作半月状,内室大,基貭密度低,属比较原始的胚胎型。 4.嗜碱质在两种外胚层及间充貭细胞均以弥散的核朊粒为主,由5—10余粒组成小群落。这些群落在尖端增厚外胚层较大、较密,粒亦较多。普通外胚层细胞的有粒内貭网或动貭较间充貭为多,二者均系胚胎型,是分散的小泡或小管。尖端增厚外胚层的...

用小白鼠胚胎阴栓日龄11—13天的前肢芽和后肢芽,锇酸固定,甲基丙烯酸甲酯及甲基丙烯酸丁酯包埋,在SEM Ⅲ型电子显微镜观察了肢芽尖端增厚外胚层、邻近普通外胚层及间充质细胞。部分材料用过锰酸钾固定或锇酸固定后氢氧化铅染色。各种细胞相互比较的结果如下: 1.两种外胚层的高尔基体极相似,均由小泡及小管组成,无大泡,横切时成为成群的小泡。普通外胚层细胞的高尔基体呈S形,增厚外胚层的马蹄形。 2.线粒体在普通外胚层卵圆形或圆形,嵴不整齐。增厚外胚层的线粒体在浅层细胞少,结构与普通外胚层相似,但愈至深层则细长的线粒体愈多。细长线粒体的嵴较密,亦较整齐,与表面垂直,基貭亦较致密。在尖端增厚外层细胞见到由胞质新形成的小泡过渡到小圆的线粒体。 3.无论在普通外胚层下或在尖端增厚外胚层下的间充质细胞,其线粒体的形状和结构相同,都是圆或卵圆,嵴少而靠近表面作半月状,内室大,基貭密度低,属比较原始的胚胎型。 4.嗜碱质在两种外胚层及间充貭细胞均以弥散的核朊粒为主,由5—10余粒组成小群落。这些群落在尖端增厚外胚层较大、较密,粒亦较多。普通外胚层细胞的有粒内貭网或动貭较间充貭为多,二者均系胚胎型,是分散的小泡或小管。尖端增厚外胚层的动貭有独特形态,即池的一端扩张成大泡,内含网状致密物,显然含有较多的,要不是特异的蛋白貭。这种扩张动貭对于胚胎分化及诱导的关系曾加讨论。 5.动质的形成有证据表明是先从核朊粒群落出现膜成为动貭小泡或小管,再并合成较大的小泡和较长的小管。 6.增厚外胚层细胞的另一特点是比较普逼的存在着形状、大小及密度不同的致密体。有的完全致密,有的泡状,有的是二者的混合体,有的界限清楚,有的处于分散状态。在形态上类似溶酶体和卵子的多泡体。内含类似核朊粒的致密粒、退化线粒体及动质膜。这些致密体和细胞膜、核膜及高尔基体未显有何关系。基膜及细胞膜完好。未见增厚外胚层细胞有排出、吞食或饮液现象,亦未见致密体排至中胚层,故认为这些致密体起于胞貭。对于分化诱导关系曾提出讨论。 7.细胞核及核膜在三种细胞未见有显著不同。核膜有孔,其外膜可与动貭膜相连,核仁小,紧靠核膜,未见有排出现象。氢氧化铅染色的标本有时显示染色貭有微丝,这在胞貭核朊粒群落亦可出现。 8.细胞膜完整,比较直,膜的内侧有一层致密物貭,无桥粒。

The ultrastructural observations on normal rabbit alveolar macrophage were carried out with electron microscope. The morphological aspects of lysosome, mitochondria, endoplasmic reticulum, Golgi complex, ribosome and vacuole etc. were described. The relationship between the morphology of lysosome with quality of phagosome and the degree of digestion, the possible significance on the divisional phenomenon of mitochondria in alveolar macrophage and the relationship of the cells morphology with its functional...

The ultrastructural observations on normal rabbit alveolar macrophage were carried out with electron microscope. The morphological aspects of lysosome, mitochondria, endoplasmic reticulum, Golgi complex, ribosome and vacuole etc. were described. The relationship between the morphology of lysosome with quality of phagosome and the degree of digestion, the possible significance on the divisional phenomenon of mitochondria in alveolar macrophage and the relationship of the cells morphology with its functional condition were discussed preliminarily.The phagocytic ability of normal alveolar macrophage in 10 rabbits were estimated. The observations of morphology during phagocytosis were carried out with electron microscope. The process and biologic importance of phagocytosis were discussed.

用电子显微镜观察正常家兔肺泡巨噬细胞超微结构,描述了溶酶体,线粒体、内质网、高尔基氏复合体,核蛋白体以及空泡等的形态特点,并初步讨论了溶酶体形态与吞噬物的性质及被消化程度的关系,线粒体二分裂现象在肺泡巨噬细胞中的可能意义,细胞形态与所处功能状态的关系。对10例正常家兔肺泡巨噬细胞的吞噬能力进行测定,用电镜观察吞噬活动时的形态并讨论吞噬过程的步骤及吞噬作用的生物学意义。

One approach to the study of ribosome structure and function is to. isolate mutants with altered responses to antibiotics that affect protein synthesis. To date no bacterial ribosomal mutant resistant to tobramycin has been documented. One hundred and ten tobramycin resistant mutants of Bacillus subtilis were isolated after ethylmethanesul-fonate treatment. They fall into three classes: (1) mutants were able to grow at 37℃ and at 42℃ in the presence of tobramycin. (2) mutants were unable to grow at 42℃...

One approach to the study of ribosome structure and function is to. isolate mutants with altered responses to antibiotics that affect protein synthesis. To date no bacterial ribosomal mutant resistant to tobramycin has been documented. One hundred and ten tobramycin resistant mutants of Bacillus subtilis were isolated after ethylmethanesul-fonate treatment. They fall into three classes: (1) mutants were able to grow at 37℃ and at 42℃ in the presence of tobramycin. (2) mutants were unable to grow at 42℃ in either the presence or absence of tobramycin. (3) mutants were able to grow at 42℃ only in absence of tobramycin. They grew less slowly than the wild type strain 168 and these was cross-resistance among tobramycin, gentamiein neomycin and kanamycin. Most of the mutants contained a single mutation causing tobramycin resistance and asporogeny, but some of transductants contained a second mutation which phenotypically suppressed the asporogenous character of tobramycin resistant mutation. Genetic analysis indicated that tobramycin resistant mutation was located in the strA-spcA region of Bacillus subtilis chromosome. The other mutation which conferred mutant temperature sensitive character mapped close to spcA locus. Therefore, we could obtain the order of genes in this area as cysA-strA-ery-tob-spcA-ts. The 70S ribosomal proteins of thirty-two tobramyein-resistant mutants were isolated. These were no apparent differences in the patterns obtained by two-dimensional polyacryla-mide gel eleetrophoresis of ribosomal proteins of mutants and parent 168 strain.

EMS诱变Bacillus subtilis 168,分离出110株抗托普霉素突变体,根据它们在不同温度时的生长表型可以分为三种类型。Tob~R突变对卡那霉素、新霉素和庆大霉素具有交叉抗性。90%的突变体含有一个tob~r spo~-单突变,某些Tob~R spo~+转化体附加有另一个抑制spo~-的突变。转导和转化分析指出tob~r基因位于strA和spcA之间,靠近ery~r基因。一个温度敏感突变基因(ts)紧密连锁spcA。它们的排列顺序为cysA-strA-ery-tob-spcA-ts。以双向聚丙烯酰胺凝胶电泳分析了32株突变体的核糖体蛋白质,在凝胶上没有发现有任何核糖体蛋白质的表观改变。

 
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