Based on the full-length infectious cDNA pUBF52 of Beet black scorch virus (BBSV), reverse genetic analysis was carried for gene functions and expression strategy of BBSV by means of in vitro transcription of mutant viral RNAs, GFP labeled expression.
本论文以甜菜黑色焦枯病毒(Beet black scorch virus,BBSV)侵染性全长cDNA克隆pUBF52为材料,利用体外转录物的核苷酸(nt)突变和绿色荧光蛋白(GFP)标记等技术,对BBSV不同基因的功能和基因组表达策略开展了反向遗传学研究。
For transient expression of the vp6 gene in Chenopodium amaranticolor by using Beet black scorch virus (BBSV) as a vector, the entire vp6 gene was cloned into a BBSV infectious cDNA as a substitute of the BBSV coat protein (cp)gene.
The frequency of C6*A in Mongoloid populations is usually lower than 0.5, while that of black people ranges between 0.5 and 0.6 and that of white people is higher than 0.6. The other difference between Caucasian and Mongoloid is that the former has very few C6*B2 while the frequency of C6*B2 in the latter ranges from 0.03 to 0.07.
Sequence variations of the coding and regulatory regions of 127 known genes in chromosome 21 were analyzed in samples, include DNAs of 31 individual Chinese, 4 pooled DNAs from Southern Chinese, Northern Chinese, Caucasian and Black populations as well as DNA samples from chimpanzee, gorilla, orangutan and macaque.
Results The A1/A1 genotype was the most frequent one and the A1/A2 the second. Compared with Germany,American and white South African populations, the frequency of IL 1A1 allele in Han population in Chongqing was higher, While IL A2 allele lower, which was similar with the black South African.
There was always a small fraction of white colonies (0.4～10.8%) among the black ones in the successive plating of conidia of black colored S. rimosus A-166, while very few black colonies appeared among the conidia from white colonies.
Results The immunopositive fibers with the black linear structure were observed on the anterior cerebral artery (ACA), middle cerebral artery (MCA), posterior cerebral artery (PCA), basilar artery (BA) in adult and aged rats, the density of immunoreactive fibers comes in the following order: ACA>MCA>PCA>BA.
Effects of garlic and black grape extracts on the activity of adenosine deaminase from cancerous and noncancerous human urinary
A black-scholes formula for option pricing with dividends
We obtain a Black-Scholes formula for the arbitrage-free pricing of European Call options with constant coefficients when the underlying stock generates dividends.
The impact of different interference substrate materials for improving the color travel effect was studied, and the influence of white and black background on the hue and the color travel effect were studied.
Nutrient Dynamics of Fine Roots in the Mixed Plantation of Poplar and Black Locust