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   anther 在 农艺学 分类中 的翻译结果: 查询用时:0.2秒
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anther
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  花药
    The Comparison of Endogenous Hormones in the Anther of T cytoplasmic Male Sterility, Restorer and Their Hybrid F 1
    T型不育系、恢复系和杂种F_1花药组织内源激素水平比较
短句来源
    Studies on the Anther Culture and Breeding Application of Rice
    水稻花药培养及育种应用研究
短句来源
    Research Advances in Anther Culture
    花药培养的研究进展
短句来源
    3. The heat-inducible, site-specific recombination system was used to control the expression of barnase gene in the anther of transgenic wheat. The vector with barnase gene driven by Osg6B promoter, the vector with Cre gene driven by heat shock promoter, the vector with blocking fragment between Osg6B promoter and barnase gene, the vector with barstar gene (a inhibitor for barnase gene) driven by Osg6B promoter were transferred into wheat by biolistic method.
    我们构建了水稻花药特异性启动子Osg6B驱动Barnase基因组成型表达载体、热激调控Cre重组酶基因表达载体、水稻花药特异性启动子Osg6B与Barnase基因之间带有阻断序列(两侧是loxp位点)的载体、水稻花药特异性启动子Osg6B驱动Barnase抑制基因Barstar基因表达的载体。 利用基因枪转化的方法转化小麦幼胚愈伤组织。
短句来源
    MUTANT IN TOBACCO ANTHER CULTURE INDUCED BY ~(60)COγr-RAYS
    γ射线诱变烟草花药培养的突变体
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  “anther”译为未确定词的双语例句
    The content of GA 4 and GA 3 in the anther of F 1 and restorer was higher than sterile line.
    F1和恢复系中GA4和GA3水平都高于不育系。
短句来源
    Correlation analysis of agronomic characters between different wheat progeny derived form anther cultured in vitro
    小麦花培后代农艺性状的相关分析
短句来源
    And barnase had one-base difference at 3'-region from the two reported sequences. But peptide sequences of them were completely identical. An anther tapetum-specific expression promoter, pTA29, was cloned by PCR method and added to 5'-terminal of barnase & barstar.
    DNA序列分析表明,pTA29和barstar片段均与已发表的序列完全一致,barnase片段在3′端和已见报道的两种序列都有一个碱基的差异,但由此推断的氨基酸序列是一致的,不影响其在植物中的表达。
短句来源
    The anther points out that felling trees for cultivating wasteland and planting at steep hills are the thief teasons of soil erosion, and at the same time the development of handicraft and mining as well as tree-chopping and military activities should not be neglected.
    本文分析了造成水土流失的人为原因:砍伐林木垦荒和陡坡种植扩大耕地是水土流失加剧的主要原因,手工业和采矿业的发展,以及大量樵采和军事行动造成的水土流失也不可忽视。
短句来源
    The new anther culture variety Zhao Hua Ai was bred and applied into the rice production.
    选育出花培新品种“朝花矮”并应用于生产。
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  anther
Its anther wall is composed of four layers of cells, and its tapetum is of the secretory type.
      
The plants forming additional pods in their flower (pistillody) have higher flavonol-degrading activity as compared to the anther-in-petal and doubleness mutants
      
Morphogenetic Effects of the Interaction of Floral Mutations Petal-Sepal and Anther in Petal in Papaver somniferumL.
      
The morphogenetic effects of the interaction of the floral mutations petal-sepal and anther in petal in Papaver somniferumL.
      
Onokhoiskaya) and triticale (2n= 56) using anther culture at different concentrations of 2,4-D in the growth medium were studied.
      
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The tobacco anthers at uninucleate eccentric stage were irradiated by 60CoY-rays for the purpose of inducing desirable mutants.The results showed that the induction frequency of plantlets increased following 1kR of 60Co γ-rays treatment.Howevr,the time of plantlet induction was delayed and the percentage of responding anthers as well as the number of plantlets induced per anther significantly decreased after 3kR of 60Co γ-ray radiation which was considered as a semilethal exposure.The plantlet numbers...

The tobacco anthers at uninucleate eccentric stage were irradiated by 60CoY-rays for the purpose of inducing desirable mutants.The results showed that the induction frequency of plantlets increased following 1kR of 60Co γ-rays treatment.Howevr,the time of plantlet induction was delayed and the percentage of responding anthers as well as the number of plantlets induced per anther significantly decreased after 3kR of 60Co γ-ray radiation which was considered as a semilethal exposure.The plantlet numbers induced per anther were extremely low following 6kR of 60Coγ-ray radiation.A white flower mutant appeared in the induced progenies.-The tobacco leaf quality of,this mutant were significa'ntly imporoved as compared with its parental line.The mutant line has been tested and proved to have commercial value though the resistance to the black shank of tobacco slightly decreased as compared with the parental line.

本研究利用~(60)Coγ射线辐照烟草单核靠边期的花药,诱导培养突变体。结果表明,1kR左右的γ射线对花粉的出苗率有促进效应;3kR时,花药出苗时间延长,出苗花药率和花药产苗率明显降低,视为半致死剂量;6kR时,虽有部分花药可以出苗,但花药产苗率极低。诱变处理当代出现白花突变体,其后代的品质比亲本品种有明显改善,但抗病力下降。经试验,该突变系在生产上有实用价值。

The cloning of pTA29,barnase and barstar as well as the construction of two plasmids: pTABNBAR55 and pTABSBAR23 were reported in this paper. pTABN-BAR55 with pTA29-barnase chimaeric gene can induce male sterility and pTABSBAR23 with pTA29-barstar chimaeric gene can restore its male fertility when transferred into the same plant. Barnase, an extracellular ribonuclease and its intracellular specific inhibitor were amplified from total DNA of Bacillus amyloliquefaciens by PCR method and cloned into pUC18 and pUC19...

The cloning of pTA29,barnase and barstar as well as the construction of two plasmids: pTABNBAR55 and pTABSBAR23 were reported in this paper. pTABN-BAR55 with pTA29-barnase chimaeric gene can induce male sterility and pTABSBAR23 with pTA29-barstar chimaeric gene can restore its male fertility when transferred into the same plant. Barnase, an extracellular ribonuclease and its intracellular specific inhibitor were amplified from total DNA of Bacillus amyloliquefaciens by PCR method and cloned into pUC18 and pUC19 separately. The two genes were sequenced by Sanger's method. The cloned barstar DNA sequence precisely corresponded to that previously reported. And barnase had one-base difference at 3'-region from the two reported sequences. But peptide sequences of them were completely identical. An anther tapetum-specific expression promoter, pTA29, was cloned by PCR method and added to 5'-terminal of barnase & barstar. Nos. ter was added to their 3'-terminal to enhance expression efficiency. Then we inserted a herbicide-resistant selecting marker "bar cassettee" into those two plasmids at Hind 111 site to form plasmids pTABNBAR55 and pTABSBAR23 respectively. The plant transformed with pTABNBAR55 will express barnase protein in anther tapetum cells and the plant will show male sterility while its RNase activity destroys anther tapetum. But its male fertility can be restored to this engineered male sterile plant by crossing it with the engineered fertility restore line which has been transformed by pTABSBAR23. The expression of barstar will suppress barnase's cytotoxic activity by forming a barnase/barstar inactive complex. It is greatly hopeful that the construction and transformation of pTABNBAR55 and pTABSBAR23 into various crop plants would facilitate hybrid production.

本文报道了用于获得人工雄性不育系的质粒pTABNBAR55和用于获得人工恢复系的质粒pTABSBAR23的构建。用PCR的方法从烟草(Nicotiana tabacum cv.Samsun)中扩增了花药绒毡层特异表达TA29基因上游—291~+12共303bp的片段,称作TA29启动子(简写作pTA29);从解淀粉芽孢杆菌(Bacillus amyloliquefaciens)中扩增了一种RNA酶及其特异抑制剂基因barnase和barstar。DNA序列分析表明,pTA29和barstar片段均与已发表的序列完全一致,barnase片段在3′端和已见报道的两种序列都有一个碱基的差异,但由此推断的氨基酸序列是一致的,不影响其在植物中的表达。把TA29启动子分别与barnase和barstar基因连接起来构成两个嵌合基因pTA29-barnase和pTA29-barstar,在这两个嵌合基因的3′端加上了260bp的Nos终止子(Nos.ter)以增强其在植物中的表达。然后将一个2.0kb的完整除草剂抗性标记“bar cassettee”插在含有这两个基因的质粒上,作为转化和制种过程的筛选标记。若将前者导入欲作...

本文报道了用于获得人工雄性不育系的质粒pTABNBAR55和用于获得人工恢复系的质粒pTABSBAR23的构建。用PCR的方法从烟草(Nicotiana tabacum cv.Samsun)中扩增了花药绒毡层特异表达TA29基因上游—291~+12共303bp的片段,称作TA29启动子(简写作pTA29);从解淀粉芽孢杆菌(Bacillus amyloliquefaciens)中扩增了一种RNA酶及其特异抑制剂基因barnase和barstar。DNA序列分析表明,pTA29和barstar片段均与已发表的序列完全一致,barnase片段在3′端和已见报道的两种序列都有一个碱基的差异,但由此推断的氨基酸序列是一致的,不影响其在植物中的表达。把TA29启动子分别与barnase和barstar基因连接起来构成两个嵌合基因pTA29-barnase和pTA29-barstar,在这两个嵌合基因的3′端加上了260bp的Nos终止子(Nos.ter)以增强其在植物中的表达。然后将一个2.0kb的完整除草剂抗性标记“bar cassettee”插在含有这两个基因的质粒上,作为转化和制种过程的筛选标记。若将前者导入欲作杂交制种母本的植物中,可以获得雄性不育系;后者导入欲作父本的植物中,可获得恢复系。

Comparied with restorer and hybrid F 1, IAA and IPA deficiency in the sterile line was compensated in the F 1. The level of ZR in F 1 was reduced and lower than sterile line and restorer line. The content of ABA varied from development stage. At the stage of pollen abortion, its level in the anther of F 1 was as much as in the restorer and lower than sterile line. The content of GA 4 and GA 3 in the anther of F 1 and restorer was higher than sterile line.

通过对不育系、恢复系和杂种F1花药中内源激素含量的对比发现:在杂种F1的花药组织中,不育系中亏损的IAA、IPA得到了补偿并且高出了恢复系。F1中ZR水平介于不育系和恢复系之间,DHZR水平低于不育系和恢复系;ABA含量因发育时期而异,但在不育系的花粉败育时期,F1花药组织中ABA含量和恢复系相当,二者都大大低于不育系。F1和恢复系中GA4和GA3水平都高于不育系。

 
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