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streptococcus pyogenes
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  化脓链球菌
     In this paper,the in vivo protection activities of a new 14-membered macrolide, clarithromycin against Staphylococci aureus,Streptococcus pyogenes and Streptococcus pneumoniae infection were compared with that of erythromycin . Results showed that clarithromycin is 6.3~8. 6 times more protent than erythromycin when administered orally in mouse protection test against Staphylococci aureus ,Streptococcus pyogenes and Streptococcus pneumoniae.
     新的14元大环内酯clarithromycin(CRM)与红霉素(EM)对金葡球菌、化脓链球菌、肺炎链球菌、嗜血流感杆菌感染小鼠体内保护作用结果显示:口服CRM对金葡球菌、化脓链球菌及肺炎链球菌的体内抗菌作用优于EM6.3~8.6倍。
短句来源
     An open reading frame of 1206 base pairs was identified. The hasB gene product was shown to have 63 .1 % similarity with HasB of Streptococcus pyogenes and 70.6 % with that of Streptococcus uberis . In order to show that hasB expression correlated with UDP-glucose dehydrogenase activity, the hasB gene was cloned under control of the T7 promoter.
     通过hasB基因的上下游序列设计引物从兽疫链球茵的基因组中克隆出一段序列,测序结果显示其包含一个由1206个碱基组成的开放阅读框,所编码的蛋白序列同化脓链球菌和乳链球菌的UDP-葡萄糖脱氢酶蛋白序列分别有63.1%和70.6%的相似性。
短句来源
     [Results]It showed that Azithromycin sodium dihydrogen phosphate had the same antibiogram with Azithromycin and expressed strong antibacterial action to hemophilus influenza, Neisseriaceae , streptococcus pneumoniae, streptococcus pyogenes. Klebsiella pneumoniae and escherichia coli. The result of MIC_ 50 was 0.031-2 mg/L.
     [结果]研究表明,阿齐霉素磷酸二氢钠与阿齐霉素具有相同抗菌谱,对流感嗜血杆菌、奈瑟氏菌、肺炎链球菌、化脓链球菌、肺炎克雷伯氏菌和大肠杆菌等致病菌具有较好的抗菌效果,MIC50为<0.031-2mg/L。
短句来源
  化脓性链球菌
     coli, 2 94 mg·kg -1 against Klebsiella pneumoniae and 0 287 mg·kg -1 against Streptococcus pyogenes respectively.
     对化脓性链球菌97102的ED50为0-287mg·kg-1,好于对照药头孢克洛,经统计学处理,有极显著差异。
短句来源
     Results indicate that azithromycin has better activity aqainst susceptible Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Streptococcus faecalis, Neisseria gonorrhoeae, Haemophilus influenzie and Bacteroidis fragilis (MIC 50 <0.016 ̄0.5mg/L).
     结果表明:azithromycin对敏感的金葡球菌、肺炎链球菌、化脓性链球菌、淋球菌、嗜血流感杆菌、粪链球菌及脆弱拟杆菌等均具有较好的抗菌作用(MIC50<0.016~0.5mg/L)。
短句来源
     Stones of 2 patients (667%) harbored bacterial gene fragments with similarity of Streptococcus pyogenes.
     2例(6.67%)DNA片段与化脓性链球菌相关;
短句来源
     gatifloxacin showed greater activity against gram-positive bacteria than ciprofloxacin and ofloxacin. The MIC90 of gatifloxacin against MSSA, Streptococcus pyogenes and Streptococcus pneumonia were 0.125, 0.125 and 0.5mg. L1, respectively.
     盐酸加替沙星对革兰阳性球菌的抗菌作用比盐酸环丙沙星与氧氟沙星增强,对甲氧西林敏感的金葡菌、化脓性链球菌和肺炎链球菌MIC90值分别为0.125、0.125和0.5mg·L-1,与司帕沙星相同;
短句来源
     Stones of 2 patients(5 . 5 6 % ) harbored bacterial gene frag- ments with similarity with Streptococcus pyogenes.
     2例 ( 5.56% )胆石中残留的细菌 DNA片段与化脓性链球菌相关 ;
短句来源
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  酿脓链球菌
     Rates of S. epidermidis, Enterococcus faecium, Streptococcus pyogenes were 60.7%,14.3%,and 7.1% in non-diagnosed patients.
     表皮葡萄球菌、粪肠球菌、酿脓链球菌在临床未确诊病例中分别为60.7%、14.3%、7.1%;
短句来源
     The prominent contaminants were as follows: coagulase-negative staphylococci , Streptococcus pyogenes, Micrococcus and Gram-positive rods.
     主要的污染菌有凝固酶阴性葡萄球菌、酿脓链球菌、微球菌属和革兰阳性杆菌。
短句来源
  “streptococcus pyogenes”译为未确定词的双语例句
     Cloning and sequence analysis of the DNase B gene of group A Streptococcus pyogenes
     A组β-溶血性链球菌DNase B基因序列的克隆与序列分析
短句来源
     Objectives To construct a recombinant vector containing fragments of emm 1 genes and emm 3 gene that are the type specific epitopes of streptococcus pyogenes.
     目的构建含A组链球菌M蛋白基因(emm基因)1型和3型特异性抗原决定簇基因的重组质粒。
短句来源
     The results of 11 antibiotics susceptibility test shows:the Streptococcus pyogenes has resistance to Kanamycin(30ug),Nalidixic acid(30ug),Streptomycin(10ug)and Sulphonamides(300ug),and is a local strain with resistance to mang antibiotic.
     11种抗生素药敏试验显示,该株菌对卡那霉素(30ug),萘啶酮酸(30ug), 链霉素(10ug)和磺胺异噁唑(300ug)都耐药。 结论 该菌株是一株具有多重耐药性的地方株。
短句来源
     ml-1 as SMM minimum effective blood concentration(Streptococcus pyogenes), sustaining time of effective blood concentration of 100 and 50mg.
     ml~(-1),100mg.
短句来源
     The MICs of sparfloxacin for 90% of gram positive organisms were 0.125 to 0.5mg/L, the inhibi tory rates against Staphylococcus aureus, Staphylococcus epidermidis and Streptococcus pyogenes all were 100% and were higher than those of ofloxacin, ciprofloxacin.
     司帕沙星对革兰阳性菌的MIC90为0.125~0.5m g/L,对金葡球菌、化脓链球菌的抑菌率均为100% ,强于氧氟沙星、环丙沙星;
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  streptococcus pyogenes
halodurans for the TnrA regulon; and in Lactococcus lactis, Lactobacillus plantarum, Streptococcus pyogenes, S.
      
Since the middle of the 1980s,? reports of invasive infections caused by streptococcus pyogenes are increasing world wide.
      
In the study, all 10 patients (seven females/ three males, average age 45.0 [4-71]) of the district hospital of Reutlingen were included who had a proven streptococcus pyogenes infection between Sept.
      
Streptococcus pyogenes was cultured from the empyema.
      
Als wichtigste Keime fanden sich: Streptococcaceae (Streptococcus pyogenes, seltener Streptococcus faecalis und vergrünende Streptokokken), Pseudomonadaceae, Enterobacteriaceae, Micrococcaceae (hier besonders Micrococcus aureus).
      
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Summary The growth requirements of the causal agent of enzootic pneumonia of swine are so fastidious that for seventeen years, since recognizaton of it as an entity by pullar (1948), it had been assumed to be a vitus, until Goodwin and Whittlestone in England, Mare and Switzer in America, near at the same time (1965), could cultivate ie With living cell free media and nominated it as Mycoplasma suipneumonia and M. hyopheumonia respectively. There after, more complex modified media were used to promote facility...

Summary The growth requirements of the causal agent of enzootic pneumonia of swine are so fastidious that for seventeen years, since recognizaton of it as an entity by pullar (1948), it had been assumed to be a vitus, until Goodwin and Whittlestone in England, Mare and Switzer in America, near at the same time (1965), could cultivate ie With living cell free media and nominated it as Mycoplasma suipneumonia and M. hyopheumonia respectively. There after, more complex modified media were used to promote facility for isolation or subcultivation. In recent years, modified Switzer′s medium Jiangsu medium No.2 (KM2), composed of 50% Eagle′s solution, 30% of 1% lactoalbumin hydrolysate, 20% normal swine serum inactivated at 56℃ for 30 minutes, with additional 1% fresh yeast extract, penicillin 500 unit/ml, thallium acetate 0.0125% and Pheol red 0.002% has been widely used in our country for isolation and subcultivation with more satisfactory results as compared With other media. Yet the costive amino-acids and biotic materials and laborious procedure in prepation of fresh yeast extract expensed more money and much time. Hence, we have tried to use Streptococcus pyogenes or Staphylococcus aureus culture preparation as substitute for Eagle′s solution and yeast extract. The procedures are as follows: 1. Martin broth is inoculated with selected pure culture of Streptococcus pyogenes (or Staphylococcus aureus) and incubated in 37℃ for 24 hours. 2. Centrifuge the culture at 2500 R.P.M. for 30 minutes. 3. Filter the supernatant through sterile seitz filter with F.K. disc under the negative pressure not above 25cm. mercury. 4. Grind the precipitated bacterial ceils with silicon carbide to homogeneous pulp, mix with aliquot of the Cultural supernatant, then filter the mixture through the same seitz filter already used. The filtrate is inocuialed in martin broth and agar slant to test the sterility. The sterile filtrate is dispensed to a number of bottles and kept in frozen state ready for use. The experimental results poited out: 1. The components in bactereal filtrate that promote growth of Mycoplasma suipneumonia are heat labile, though not completely damaged by 56℃ for 30 minutes to Streptococcus and 70℃ 30 minutes to Staphylococcus and do not resist autoclave temperature. 2. The beneficial components to growth of M. suipneumonia exsist both in the cultural fluid and bacterial cells. 3. Survival time: in 37℃, KM_2 culture last at 4th day, the cultures of the media with Strptococeus substitute (Str_50) or Staphylococcus substitute (Sa_50) all last at 8th day; in 4-8℃, KM_2 culture last at 12th day, the cultured cf str_50 last at 26th day and the culture of Sa_50 last at 30th day. 4. Once a certain inoculum of M. suipneumonia added to KM_2 did not show growth any more, but could grow in KM_2 containing 10% Streplococcus preparation. This may be a hint that the preparation of Streptococcus might contain some factors possessing the ability of reviving M. suipneumonia at a critical point. 5. There are no visible differences between KM_2, Str_50 and Sa_50 cultures in ceils′ morphology and pathogenicity, but somewhat diversities in growth rate. 6. Martin broth could be used as substiute instead of 1% lactoalbumin hydrolysate soltrion for subcultivation of M. suipneumonia.

一、以50%的化脓性链球菌或黄金色葡萄状球菌的马丁汤培养物的无菌过滤液(包括过滤的菌体微粒)(代号分别为Str和Sa)、30%的1%水解乳蛋白溶液,20%的正常猪血清(56℃灭活30分钟)(附加物如青霉素500单位/毫升,醋酸铊0.0125%,酚红0.002%)组成的液体培养基,酸度校正至pH7.2~7.4,培养猪肺炎支原体,生长良好。也可用作含1.2%高纯度琼脂固体培养基的基础。含50%链球菌液培养基代号为Str_(50),含50%葡萄状球菌的代号为Sa_(50)。二、在生长旺盛后保存于37℃中,Str_(50)及Sa_(50)培养物均存活8天,而KM_2(由50%的Eagle氏液,30%水解乳蛋白溶液,20%灭活正常猪血清,1%酵母抽出液和附加物如青霉素500单位/毫升,醋酸铊0.125%,酚红0.002%组成)的培养物仅存活4天。在4——8℃冰箱中,Str50培养物存活26天,Sa50培养物存活30天,而KM_2培养物仅存活12天。三、供给生长的成份既存于菌液,也存在于菌体。四、供给生长的成份不耐高热蒸汽灭菌。五、链球菌以65℃30分钟灭活,葡萄状球菌以70℃30分钟灭活,生长成份尚未完全破坏,但不如未...

一、以50%的化脓性链球菌或黄金色葡萄状球菌的马丁汤培养物的无菌过滤液(包括过滤的菌体微粒)(代号分别为Str和Sa)、30%的1%水解乳蛋白溶液,20%的正常猪血清(56℃灭活30分钟)(附加物如青霉素500单位/毫升,醋酸铊0.0125%,酚红0.002%)组成的液体培养基,酸度校正至pH7.2~7.4,培养猪肺炎支原体,生长良好。也可用作含1.2%高纯度琼脂固体培养基的基础。含50%链球菌液培养基代号为Str_(50),含50%葡萄状球菌的代号为Sa_(50)。二、在生长旺盛后保存于37℃中,Str_(50)及Sa_(50)培养物均存活8天,而KM_2(由50%的Eagle氏液,30%水解乳蛋白溶液,20%灭活正常猪血清,1%酵母抽出液和附加物如青霉素500单位/毫升,醋酸铊0.125%,酚红0.002%组成)的培养物仅存活4天。在4——8℃冰箱中,Str50培养物存活26天,Sa50培养物存活30天,而KM_2培养物仅存活12天。三、供给生长的成份既存于菌液,也存在于菌体。四、供给生长的成份不耐高热蒸汽灭菌。五、链球菌以65℃30分钟灭活,葡萄状球菌以70℃30分钟灭活,生长成份尚未完全破坏,但不如未灭活的生长旺盛。六、加10%的Str于KM_2中有促进猪肺炎支原体生长和复苏作用。用于培养棉花拭子深擦病猪喉头和鼻道的滤液,分离率可达77.8%。七、Str_(50)和Sa_(50)制造方法较简单,成本低廉,组成成份容易获得,宜于一般实验室应用和大量生产。八、Sa_(50)中30%的水解乳蛋白溶液可以马丁汤代替,用于接种传代。

The susceptibility of clinically isolated Gram positive and Gram negative bacteria to 17 kinds of β-lactam antibiotics were determined by agar dilution method. Cephalosporins of first generation and imipenem possessed powerful antibacterial action against most Gram positive bacteria, but their actions against Streptococcus pneumoniae, Streptococcus pyogenes and Streptococcus viridans were not superior than penicillins. Enterococcus was resistant to all cephalosporins tested, relatively more...

The susceptibility of clinically isolated Gram positive and Gram negative bacteria to 17 kinds of β-lactam antibiotics were determined by agar dilution method. Cephalosporins of first generation and imipenem possessed powerful antibacterial action against most Gram positive bacteria, but their actions against Streptococcus pneumoniae, Streptococcus pyogenes and Streptococcus viridans were not superior than penicillins. Enterococcus was resistant to all cephalosporins tested, relatively more sensitive to imipenem and only half of the strains was sensitive to ureidopenicillins. Cephalosporins of third generation and the three atypical β-lactams possessed very strong antibacterial actions against bacteria of Enterobacteriaceae, remarkably stronger than the 5 penicillins and gentamicin. H. influenzae was sensitive to all of the antibiotics used in this study. Acinetobacter anitratus was relatively resistant and only imipenem, ceftazidime, cefotaxime, ceftriaxone and piperacillin had antibacterial action against certains strains of A. anitratus. About half of the Mima polymorpha was sensitive to penicillins, cephalosporins of second and third generation (except cefoperazone) and imipenem. Ceftazidime possessed the most powerful action against Ps aeruginosa, next were cefoperazone and piperacillin. Furbenicillin, azlocillin, aztreonam, imipenem and ceftriaxone possessed certain degrees of action also. With the exception of cefoperazone, cephalosporins of third generation and imipenem had relatively strong actions against Alcaligenes faecalis.

本文报道17种β-内酰胺类抗生素对临床分离的424株革蓝氏阳性和阴性菌的体外抗菌作用.第一代头孢菌素和亚氨甲基thienamycin对多数革蓝氏阳性菌有强大抗菌作用,但对肺炎链球菌、化脓性链球菌和草绿色链球菌的活性并不优于青霉素类抗生素.肠球菌对头孢菌素类抗生素皆耐药,对亚氨甲基thienamycin比较敏感,半数菌株对脲基青霉素敏感.第三代头孢菌素和3种非典型β-内酰胺类抗生素对肠杆菌科细菌有极强抗菌活性,明显优于5种青霉素类抗生素和庆大霉素.流感杆菌对本实验采用的所有抗生素均敏感.硝酸盐阴性杆菌比较耐药,仅亚氨甲基thienamycin、Ceftazidime、头孢噻肟、头孢三嗪和哌拉西林对某些菌株有抗菌作用.多形模仿菌对5种青霉素类抗生素、第二代、第三代头孢菌素类抗生素(除头孢哌酮外)和亚氨甲基thienamycin敏感者约占半数.Ceftazidime对绿脓杆菌的作用最强,次为头孢哌酮和哌拉西林,再次为味苄青霉素、阿洛西林、Aztreonam、亚氨甲基thienamycin和头孢三嗪.除头孢哌酮外,第三代头孢菌素类抗生素和亚氨甲基thienamycin对产碱杆菌有较强活性.

The lipoteichoic acid (LTA) extracted from group A Streptococcus pyogenes was purified,and was identified by comparing its infrared spectrum with that of LTA obtained by Ofek(1975). SRBC-sensitizing activity of LTA was assayed with the method of indirect RBC agglutination, LTA-sensitized SRBC could be agglutinated by both anti-LTA antibodies and anti-streptococcus antibodies, that showed LTA was one of group A streptococcus antigens.

用冷酚抽提法从A群溶血性链球菌中提取了脂磷壁酸(Lipoteichoic acid LTA),经紫外扫描、凝胶过滤对提取物的纯度进行了分析。经红外光谱分析同文献报道相似。间接血球凝集试验结果显示LTA能自主粘附于经双醛法固定的羊红血球,并能同抗LTA抗体或抗A群链球菌抗体结合而使血球凝集。LTA注射小鼠腹腔,能使其脾重显著增加。抗肿瘤效力实验证明LTA能够抑制艾氏癌细胞在小鼠体内(皮下、腹腔)的生长。接种2×10~5个细胞于小鼠皮下后连续7天注射含10μg磷的LTA其抑瘤率为81.9%,与对照组相比(P<0.01)呈显著性差异。当对腹水型动物注射含0.25μg和1μg的LTA时其生命延长率分别为54%和44%。并进一步证明LTA抗肿瘤的机制之一是诱导肿瘤坏死因子TNF的产生。

 
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