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streptococcus pyogenes
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  “streptococcus pyogenes”译为未确定词的双语例句
    Cloning and sequence analysis of the DNase B gene of group A Streptococcus pyogenes
    A组β-溶血性链球菌DNase B基因序列的克隆与序列分析
短句来源
    Objectives To construct a recombinant vector containing fragments of emm 1 genes and emm 3 gene that are the type specific epitopes of streptococcus pyogenes.
    目的构建含A组链球菌M蛋白基因(emm基因)1型和3型特异性抗原决定簇基因的重组质粒。
短句来源
    Methods emm gene was sequenced for the 104 isolates of Streptococcus pyogenes collected from school children in China from 1988-1994 and compared with their T type.
    方法 用引物以PCR法扩增GASM蛋白的N末端并进行测序 ,确定GAS的emm型 ,并与T分型对照比较。
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  相似匹配句对
    Cloning and sequence analysis of the DNase B gene of group A Streptococcus pyogenes
    A组β-溶血性链球菌DNase B基因序列的克隆与序列分析
短句来源
    Stones of 2 patients (667%) harbored bacterial gene fragments with similarity of Streptococcus pyogenes.
    2例(6.67%)DNA片段与化脓性链球菌相关;
短句来源
    Architecture of Streptococcus mutans biofilms
    变异链球菌生物膜结构观察
短句来源
    Culture of Group B Streptococcus and Its Optimization
    B群链球菌的培养及优化研究
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  streptococcus pyogenes
halodurans for the TnrA regulon; and in Lactococcus lactis, Lactobacillus plantarum, Streptococcus pyogenes, S.
      
Since the middle of the 1980s,? reports of invasive infections caused by streptococcus pyogenes are increasing world wide.
      
In the study, all 10 patients (seven females/ three males, average age 45.0 [4-71]) of the district hospital of Reutlingen were included who had a proven streptococcus pyogenes infection between Sept.
      
Streptococcus pyogenes was cultured from the empyema.
      
Als wichtigste Keime fanden sich: Streptococcaceae (Streptococcus pyogenes, seltener Streptococcus faecalis und vergrünende Streptokokken), Pseudomonadaceae, Enterobacteriaceae, Micrococcaceae (hier besonders Micrococcus aureus).
      
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We amplified recombinant streptokinase (rSK) gene by PCR from the chromosomal DNA of Streptococus pvogenes group A and expressed it in the expression vector pBV220, and the expressed rSK amounted to 60% of total celi protein. The final purity of the rSK reached above 95% after purification by gel filtration and anion - exchange chromatography. The specific activity was 1. 3 × 105 U/mg determined by tbe fibrinogen plate technique. Six hybridoma clbnes which secreted monoclonal antibodies against rSK were obtained....

We amplified recombinant streptokinase (rSK) gene by PCR from the chromosomal DNA of Streptococus pvogenes group A and expressed it in the expression vector pBV220, and the expressed rSK amounted to 60% of total celi protein. The final purity of the rSK reached above 95% after purification by gel filtration and anion - exchange chromatography. The specific activity was 1. 3 × 105 U/mg determined by tbe fibrinogen plate technique. Six hybridoma clbnes which secreted monoclonal antibodies against rSK were obtained. The rSK gene was cloned into the vector M13 mp19 for DNA sequencing. The nucleotide sequenee more or less differs from the previously reported and it shares 91% homology with another SK gene SPSKA isolated from another strain of Streptococcus pyogenes, and 87% homology with SK gene SESKG from Streptococcus equsimilis group C.

用PCR方法从链球菌染色体基因组中分离了链激酶基因,并把它克隆到表达载体pBV220中。诱导表达后重组蛋白以包涵体的形式存在并占菌体总蛋白的60%以上。纯化后的重组蛋白的纯度达90%以上。血纤维蛋白原平板测活方法表明,重组蛋白比活性为1.3×10~5U/mg。重组链激酶蛋白免疫小鼠后获得6株分泌抗链激酶单克隆抗体的杂交瘤。把重组链激酶基因克隆至M13mp19中进行DNA序列分析,结果表明与文献报道的有区别。

To search for bacterial DNA sequences in cholesterol gallstones with negative bacterial culture.We used nested primers polymerase chain reaction (NPPCR) technique to amplify bacterial gene fragments were amplified in vitro from DNA extracted from chloesterol gallstones.Comparative 16S ribosomal RNA sequence analysis was used for elucidation of bacterial identification.The gallbladder gallstones of 30 patients were analysed.Bacterial DNA was found in the stones of 26 patients. There was no difference either...

To search for bacterial DNA sequences in cholesterol gallstones with negative bacterial culture.We used nested primers polymerase chain reaction (NPPCR) technique to amplify bacterial gene fragments were amplified in vitro from DNA extracted from chloesterol gallstones.Comparative 16S ribosomal RNA sequence analysis was used for elucidation of bacterial identification.The gallbladder gallstones of 30 patients were analysed.Bacterial DNA was found in the stones of 26 patients. There was no difference either in cholesterol and water content or in harboring bacterial DNA of gallstones.E.colirelated DNA fragments were found in the stones of 8 patients (2667%).Propionibacteria type DNA was found in the stones of 7 patients (2333%).Stones of 2 patients (667%) harbored bacterial gene fragments with similarity of Streptococcus pyogenes.A more heterogenous sequence collection was found in 7 patients (2333%) and could be assigned to the multiple bacterial infections.Another stones of 2 patients (667%) had bacterial DNA with lower molecularweight which might be related to some unidentified bacteria.The results suggested that most cholesterol gallstones harbor bacterial DNA.It is important to determine whether these microorganisms are innocent bystanders or active participants in cholesterol gallstone formation.

为探讨微生物在胆固醇结石形成中的作用,作者从30例胆汁细菌培养阴性的胆囊胆固醇结石中提取DNA,应用套式聚合酶链反应(NP-PCR)技术从中特异性地扩增细菌DNA片段。结果显示26例(86.67%)胆固醇结石中有细菌DNA存在。此外,用16SrRNA基因序列对照分析鉴定细菌种类,8例(26.67%)为与大肠杆菌相似的DNA片段;7例(23.33%)为痤疮丙酸杆菌型DNA序列;2例(6.67%)DNA片段与化脓性链球菌相关;7例(23.33%)DNA片段具有多样性,可能有多种细菌混合感染;另外2例(6.67%)DNA分子量较低,归类于其它未鉴定细菌。作者认为多数胆固醇结石内有细菌DNA存在,但这些微生物的确切作用,有待于进一步深入研究。

AIM: To amplify and analyze the differential DNA fragment between pathogenic leptospira serovar lai and nonpathagenic leptospira serovar patoc I. METHODS: The previously subtractive DNA fragment only existing in leptospira serovar Lai was amplified by cassette ligation and semi-nested PCR.The obtained gene was sequenced and searched homologically. In addition, the deduced amino acid was analyzed and the secondary stracture of protein was predicted. RESULTS: The 580 bp DNA fragment, which deposited in GenBank...

AIM: To amplify and analyze the differential DNA fragment between pathogenic leptospira serovar lai and nonpathagenic leptospira serovar patoc I. METHODS: The previously subtractive DNA fragment only existing in leptospira serovar Lai was amplified by cassette ligation and semi-nested PCR.The obtained gene was sequenced and searched homologically. In addition, the deduced amino acid was analyzed and the secondary stracture of protein was predicted. RESULTS: The 580 bp DNA fragment, which deposited in GenBank (AF495587), was cloned, and four overlapping open reading frames (ORF) was contained. The high homology with conserved hypothetical protein streptococcus pyogenes was found. CONCLUSION: This study lays foundation for deeply exploring biological actions of new gene and pathogenic mechanism of leptospira serovar lai.

目的 :筛选致病钩端螺旋体赖型 0 1 7株毒力相关基因DNA差异片段 ,并进行核苷酸及基因信息学分析。方法 :提取钩端螺旋体 0 1 7株基因组DNA ,根据抑制消减杂交 (SSH)筛选的 0 1 7株特有的 1 53bp核苷酸短片段 ,采用盒式连接半巢式PCR技术 ,扩增相邻未知序列 ,进行序列测定、分析及同源性检索 ,并进行蛋白二级结构预测。结果 :克隆获得 580bp核苷酸长度的基因片段 ,包含 4个重叠开放阅读框架 (ORF) ,在氨基酸水平上与A型化脓性链球菌 ,肺炎链球菌保守的假想蛋白高度同源。被GenBank收录 ,收录号为AF495587。结论 :本研究筛选并分析了可能是钩体毒力相关的基因片段 ,为进一步探讨该基因的生物学功能及阐明赖型钩端螺旋体致病分子机制打下了基础。

 
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