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chitinase
相关语句
  几丁质酶
    Taxonomic Identification of a Biocontrol Agent Streptomyces sp. Men-myco-93-63 Against Verticillium Dahliae and Characterization of Its Chitinase Genes
    拮抗链霉菌Men-myco-93-63分类学鉴定及其几丁质酶基因研究
短句来源
    Functional Analysis of the Baculovirus Chitinase Gene
    杆状病毒几丁质酶基因功能分析
短句来源
    The Study on the Chitinase Gene and the Antibiotic Biosynthesis Gene Cluster of Streptomyces Roseoflavus
    玫瑰黄链霉菌几丁质酶基因及抗生素合成基因簇研究
短句来源
    CHITINASE OF THE FUNGUS BEAUVERIA BASSIANA
    白僵菌Beauveria bassiana的几丁质酶
短句来源
    Chitinase in Viola faba leaves:induction by APhis craccivora──a convergent plant physiological stress reaction
    蚕豆叶片几丁质酶活性的蚜虫诱导──植物生理应激反应的趋同性
短句来源
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  几丁酶
    THE RELATIONSHIP BETWEEN OCCURRENCE OF ANTHRACNOSE AND CHITINASE AND β- 1 ,3-GLUCANASE OF BANANA FRUIT AFTER HARVEST
    香蕉采后炭疽病发生与几丁酶和β-1,3-葡聚糖酶的关系
短句来源
    Relationship between Chitinase and Plant Disease Resistance
    植物几丁酶与抗病性的关系
短句来源
    Construction of a Fusing Chitinase and Protease Gene and Improvement of Mycoinsecticide in Beauveria Bassiana
    几丁酶—蛋白酶融合基因的构建与球孢白僵菌毒力的提高
短句来源
    stolonifer by producing lytic enzymes such asβ-1,3-glucanase and chitinase but also induced the systematic resistance of the fruits.
    葡聚糖酶和几丁酶与病原茵直接作用,并参与诱导寄主产生抗性等。
短句来源
    Protease gene CDEP-1 and chitinase gene Bbchitl were firstly expressed in yeast.
    进而将球孢白僵菌Pr1类蛋白酶基因CDEP-1分别与几丁酶基因Bbchit1和Machit1融合,分生孢子电击转化法将融合基因导入球孢白僵菌中。
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  “chitinase”译为未确定词的双语例句
    The purified chitinase functioned optimally at 40℃ and pH 5. 5 ,and was stable within a broad range of pH 5 -8.5 and below 30℃.
    其最适反应温度为40℃,在30℃以下很稳定; 最适pH值为5.5,在pH 5-8.5范围内均较稳定;
    The chitinase activity was inhibited by Hg2+ ,Fe3+ ,Zn2+ ,Cu2+ and Mg2+ and slightly stimulated by Na2+.
    酶活性受Hg2+、Fe3+、Zn2+、Cu2+、Mg2+等金属离子不同程度的抑制。
    The Km and Vmax values for the chitinase, using colloidal chitin as substrate, were 1. 72mg ml-1 and 21.18 U ml-1, respective-
    Na+对酶有轻微的激活作用; 以胶体几丁质为底物时,该酶的米氏常数Km为1.72mg ml-1,最大反应速度Vmax为21.18 U ml-1。
    In light of the polyphasic taxonomical principle, Streptomyces sp. Men-myco-93-63 is accordingly named as Streptomyces roseoflavus Men-myco-93-63.To align the sequences of chitinase genes published in GeneBank using Clustal V progeamme, 39 Streptomyces chitinase genes are mainly clustered into 3 groups, i.e. Group A, B, and C.
    因此,根据链霉菌多相分类原则,将链霉菌Men-myco-93-63定名为玫瑰黄链霉菌Men-myco-93-63(Streptomyces roseoflavus Men-myco-93-63)。
短句来源
    2. Treatment of harpin at 90 mg/L increased activity of peroxidase (POD), chitinase (CHT), phenylalanine ammonia-lyase (PAL), chalcone isomerase (CHI) and polyphenoloxidase (PPO) in Hami melon(cv. 8601).
    2.90mg/L的Harpin处理可有效提高哈密瓜(品种:8601)的POD和CHT活性,果实的PAL、CHI和PPO活性可被Harpin处理明显诱导。
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  chitinase
Extracellular proteinase and chitinase produced by a culture ofStreptomyces kurssanovii
      
Extracellular enzymes-a chitinase and a proteinase with molecular weights 22 and 32 kDa, respectively-were isolated fromStreptomyces kurssanovii cells.
      
-The cultivation conditions of wild-type strain V-10 and mutant strain M-l (overproducer of endonuclease and chitinase) ofSerratia marcescens optimal for extracellular lipase biosynthesis were determined.
      
Synthesis of Extracellular Chitinase by Wild-Type B-10 and Mutant M-1 Strains of Serratia marcescens
      
In the absence of chitin inductors, chitinolytic enzymes were not found in the culture liquid of B-10, whereas M-1 cells produced the chitinase complex (to 470 pU/cell).
      
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Laboratory screening and selection of Beauveria bassiana against peach fruit borer, Carposina nipponensis, were carried out in Beijing during 1988-89. Seventy strains were collected from Liaoning, Henan and Shandong Provinces and were inoculated on the 3rd instar larvae of C. nipponensis. Three strains selected (46-1, 46-2 and 37-3) had infectivities of 96.7%, 92.8% and 93.3%, respectively, which were higher than that of other strains. The growth character and chitinase activity of the collected B. bassiana...

Laboratory screening and selection of Beauveria bassiana against peach fruit borer, Carposina nipponensis, were carried out in Beijing during 1988-89. Seventy strains were collected from Liaoning, Henan and Shandong Provinces and were inoculated on the 3rd instar larvae of C. nipponensis. Three strains selected (46-1, 46-2 and 37-3) had infectivities of 96.7%, 92.8% and 93.3%, respectively, which were higher than that of other strains. The growth character and chitinase activity of the collected B. bassiana strains were studied with four strains representing high, medium, and low toxicities to the host insect. The results indicated that the infectivity of B. bassiana strains tested had a significant positive correlation with the quantity of mycelia growth, chitinase activity and spore productivity.

将辽宁、河南、山东等地采集到的70个寄生于桃小食心虫的球孢白僵菌菌株以3龄桃小食心虫进行筛选,得到了46-1,46-2和37-3三个致病率分别为96.7%、92.8%和93.3%的优良菌株。以四个代表高,中、低致病率的菌株46-1、46-2、7-1(43.3%)和66(33.3%),测定其菌丝的生长量,产孢量和几丁质酶活性的结果显示菌株的致病力与菌丝生长量、几丁质酶活性、菌株产孢量呈显著的正相关。

Recent world-wide achievments were reviewed in the use of transgenic plantsto protect plants from diseases, including the introduction of viral genome components(CPgene,replicase gene, cRNA and satRNA), ribozyme gene and anti-virus compound synthasegene into plants against viruses,pathogen-derived detoxifing enzyme gene and antibiotic pep-tide gene into plants against bacteria,and chitinase gene,glucanase gene,RIP gene and stil-bene synthase gene into plants against fungi,respectively.

综合评述了近5年世界各国在植物抗病基因工程方面取得的进展.包括转植物病毒基因组成分(CP基因、复制酶基因、反义RNA及satRNA)、转核酶基因和转植物抗生物质编码基因的抗病毒病植株;转病原细菌解毒基因、转抗菌肽基因和转溶菌酶基因的抗细菌病植株;转几丁质酶基因、转葡聚糖酶基因、转RIP基因、转芪合成酶基因的抗真菌病植株等研究所取得的成果.

The Northern blot was performed to research the systematic dynamic characteristic of PR-genes expression at mRNA for the resistant cultivar(Erik)and the susceptible line(6B699)of wheat at 8,16,24,32,40,48,56,65 and 72h after inoculation with the isolate 86-124 of Pyrenophoratritici-repentis(ptr),The results showed that the infection of the pathogen had clearly induced the expression of the genes ofβ-1,3-Glucanase(PR-2),Chitinase(PR-3)and Phenylalanine Ammonia Lvase(PAL)inboth the resistant and susceptible...

The Northern blot was performed to research the systematic dynamic characteristic of PR-genes expression at mRNA for the resistant cultivar(Erik)and the susceptible line(6B699)of wheat at 8,16,24,32,40,48,56,65 and 72h after inoculation with the isolate 86-124 of Pyrenophoratritici-repentis(ptr),The results showed that the infection of the pathogen had clearly induced the expression of the genes ofβ-1,3-Glucanase(PR-2),Chitinase(PR-3)and Phenylalanine Ammonia Lvase(PAL)inboth the resistant and susceptible wheat.And suggested that those genes were classified into a same non-specifical resistant group.They presented similar dynamic characteristic.i.e.the genes were induced to transcripte at 8~16 hrs after inoculation,the amount of mRNA to maximum around 48 hrs after inoculation,declied at 64 hrs postinfection.However,the tendeny of mRNA transcription were 16 hrs earlier than the development of tan spot disease symptom.

采用Northern杂交技术,对小麦抗、感品系在接种褐斑病菌(ptr)86-124小种后8,16,24,32,40,48,56,64,72小时叶片病原相关蛋白(PR)基因在mRNA水平上的表达动态进行了研究。结果表明,小麦受到病原菌的侵染后,β-1,3-葡聚糖酶,几丁酶,苯丙氨酸解氨酶等病原相关蛋白基因在抗、感品系内均被诱导表达,表现了一种非特异性的抗性反应。并且,以上3个基因的诱导表达具有大体相似的动态规律。β-1,3-葡聚糖酶(PR-2)基因在接种病原菌ptr后16小时开始表达,48小时后表达量达高峰,64小时后表达减弱;几丁酶(PR-3)基因在接种后8小时开始表达,48小时后达最大值,72小时后表达量下降;苯丙氨酸解氨酶(PAL)基因于接种后8小时被诱导表达,48小时后表达进入盛期,64小时后开始下降,但直至72小时后仍维持一定水平,这种趋势较病程的发展一般提前16小时左右。

 
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