To study the effective ingredients of traditional Chinese medicine prescription Yu ping feng, which have anti-inflammatory and immunodulatory actions, an effective part-total glucosides of Yu ping feng(TGYPF) was isolated by extraction, isolation, refinement and purification with the techniques and methods of chemistry of TCM.
本课题采用中药化学提取分离、精制纯化的技术与手段,对玉屏风散中具有抗炎免疫作用的有效成分进行研究,分离得到复方中总的有效部位——玉屏风总苷(total glucosides of Yu ping feng,TGYPF)。
The DNA purification process from the rat peripheral blood can be achieved and the DNA yield is 24 ng/(μL whole blood), which can reach the level of the commercial DNA purification kits.
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Separation, purification and structure elucidation of a polysaccharide from root of Cudrania tricuspidata
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Purification and properties of alkaline phosphatase of silkworm Bombyx mori
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The purification fold was 464 times and specified activity was 3 936 U/mg.
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Western blotting analysis showed that TM3 protein was purified with affinity purification.
An improved method for the isolation and purification of chondroitin sulfate A (ChS-A) and chondroitin sulfate G (GhS-C) from bovine trachea was described. In order to remove combined proteihs and minimize the degradation of the mucopolysac-charides, two enzymes, trypsin and papain, were used consecutively. Subsequently, the residual proteins were removed by shaking with organic solvents, treating with adsorbent and chromatographing with ion-exchange resin. The mucopolysaccharides were further purifie...
Biochemical characterization of fractions obtained from various steps of isolation and purification of calf thymic peptide factors was reported. Experimental results showed that by using Sephadex G-15, peptides could be separated from nucleotides in the crude extract. After being further subjected to DEAE-Sephadex A-25, Sephadex G-10 and two dimensional paper chromatography, biologically active peptides could be purified to single peptides. The molecular weights of these peptides were roughly estimate...
Methods of glucagon purification from the insulin process was studied. The salt precipitate of insulin was used as starting material which was passed over a macroproporous strong acid ion-exchange resin column. Insulin was not adsorbed and flew out in the effluent (A). It was then eluted with dilute NH_4OH solution and the protein solution containg glucagon (B) was obtained. After separation of solution (B) by gel filtration, the fraction (C) was a glucagon-rich fraction. Concentrated (C) was separate...