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plaque forming cell
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  空斑形成细胞
     Ceiastrol ip 1, 3mg/kg×5d caused marked decreases of the weights of thymus and of spleen plaque forming cell (PFC) in the mice, caused marked increase in serum complement C3 level, but the level of serum hemolysin and IgG in the mice was not significantly affected.
     雷公藤红素(Celastrol)ip1,3mg/kg×5d能明显减轻小鼠胸腺的重量,降低小鼠脾脏空斑形成细胞数,同时提高血清补体C_3含量,而对小鼠血清溶血素及IgG水平无明显影响。
短句来源
     In this report, we observed the effects of Formica fusca L on phagocyte function of peritoneal, macrophage,plaque forming cell of spleen and T lymphocyte subsets of lymphatic node in mice The results showed- the phagocyte function of macrophage, the number of plaque forming, cell, the L3T4 cell, the ratio of L3T4 and Lyt2 cell in the mice of cell the experimental group were increased ( P<0.01~0.001 ) .
     本文观察了黑蚂蚁对小鼠巨噬细胞吞噬功能、空斑形成细胞数及T细胞亚群的影响。 结果表明,实验组小鼠经胃饲蚁粉液(1g/kg)10天后,巨噬细胞吞噬功能、空斑形成细胞数、L_3T_4细胞及L_3T_4/L_(yt_2)比值明显增高,与对照组相比P<0.01~0.001。
短句来源
     Results In 250mg/kg and 1 000mg/kg dose groups, all the thymus and spleen weight, the plaque forming cell count, serum hemolysin volume and toe thickness as well as carbon clearance index were significantly lower than those in control group ( P<0.05, P<0.01 ).
     结果  2 5 0、10 0 0mg/kg剂量组的胸腺和脾脏重量明显低于对照组 (P <0 0 5 ,P <0 0 1) ,空斑形成细胞对数值、血清溶血素抗体体积、足跖厚度及碳廓清指数均低于对照组 (P <0 0 5 ,P <0 0 1)。
短句来源
     Plaque forming cell (PFC) was determined with quantitative haemolysis of SRBC (QHS).
     绵羊红细胞(SRBC)的定量溶血测定法(QHS)测定脾溶血空斑形成细胞(PFC);
短句来源
     HOU by i.g at 120 mg/kg remarkably increased the development of serum hemolysin and the production of the spleen plaque forming cell (PFC) in cyclophosphamide mice.
     合成鱼腥草素 12 0mg/kg还能明显增强环磷酰胺模型小鼠血清溶血素的生成及脾脏空斑形成细胞溶血能力 .
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  抗体形成细胞
     Methods:The animal model was made by immuning mice with sheep red blood cell(SRBC), delayed type hypereneitivity(DTH) by measuring foot thickness, qutity of plaque forming cell (PFC) by hemolytic spectrophotometry, proliferation of T and B lymphocyte by MTT colorimetry, phagocytosis ability of M Φ in abdominal cavity by neural red colorimetry were observed respectively.
     方法 :用绵羊红细胞 (SRBC)免疫小鼠 ,分别用小鼠脚掌厚度测量法观察迟发性超敏反应 (DTH)、溶血分光光度法观察体外抗体形成细胞数 (PFC)、微量血凝法观察溶血素、MTT法观察脾T、B淋巴细胞的增殖功能 ,腹腔MΦ吞噬中性红比色法观察腹腔MΦ吞噬中性红的能力。
短句来源
     Analyse serum hemohysin and plaque forming cell (PFC). Test group compare with control group (P<0.05).
     血清溶血素及溶血空斑法测定体外抗体形成细胞,试验组与对照组比较( P< 005) 。
短句来源
     The effects of cytarabine on the immune function were determined with plaque forming cell (PFC) assay, hemolysin CH50 test and antigen specific rosette forming cell (ARFC) assay.
     所用的方法有体外抗体形成细胞试验,溶血素CH50的测定和抗原特异性花环形成试验。
短句来源
     Admistration of Cordycep sinensis polysaccharides to LACA mice for 15 days at the dose of 6. 85ms/kg not only enhanced delayed-type hypersensitivity reponse but also promoted the plaque forming cell(PFC)-response and hemagglutination titers against sheep red cell(SRBC ).
     实验结果表明,LACA种小鼠连续15天经口给予虫草多糖后,在6.85mg/kg剂量下可增强小鼠迟发型变态反应,提高脾脏抗体形成细胞数和血清中抗绵羊红细胞抗体效价。
短句来源
     The two groups were got blood at eye and was killed to get dilutional liquid of spleen celles after five days,To ues techniques of plaque forming cell(PFC) and Quantitative hemolysis spectrophotometry(QHS) tests the PFC rate and QHS value in two groups,significant difference was found between two groups.
     用体外抗体形成细胞实验. (Plaque forming cell,PFC)和定量溶血分光光度测定法(Quantitativehemolysisspectrophotomentry,QHS),分别测取两组的抗体形成细胞率和QHS(495nm)OD值,经X±SD组间比较的t检验,观察有无差异性.
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  溶血空斑形成细胞
     Plaque forming cell (PFC) was determined with quantitative haemolysis of SRBC (QHS).
     绵羊红细胞(SRBC)的定量溶血测定法(QHS)测定脾溶血空斑形成细胞(PFC);
短句来源
     This paper reported the effect of Panax notoginseng (PN) decoction,crude polyshaccharid on the natural killer cell (NKC), Macrophage (M_Φ), antigen-linking cell and antibody-producing cell of mice as determined dy the ~125I-UdR incoporaterelease assay, the serum lysozyme content assay, the special rosette forming cell (SRFC) test and the plaque forming cell (PFC) test.
     本文报道了应用~(125)I—UdR释放试验、溶菌酶测定、特异性玫瑰花形成细胞测定和溶血空斑形成细胞测定方法检测了三七水煎剂和三七多糖在小鼠体内对自然杀伤细咆、巨噬细胞、抗累结合细胞和抗体分泌细胞的影响。
短句来源
     Objective:To observe the effects of extract of river clam(KAL) on delayed type hypersensitivity(DTH)response and hemolytic plaque forming cell(PEC) response in immunosuppression mice.
     目的 :观察河蚌提取物 (KAL)对免疫低下小鼠的迟发性过敏反应 (DTH)和溶血空斑形成细胞 (PFC)反应的影响。
短句来源
     Methods After irradiated mice spleen cells were prepared to assay the reaction to Con A, LPS, MLC, IL - 2 production and plaque forming cell (PFC ).
     方法 UVB照射后小鼠脾细胞对丝裂原反应,混合淋巴细胞培养,溶血空斑形成细胞及脾细胞的白细胞介素-2(IL-2)产生等非特异免疫功能指标的测定。
短句来源
     Methods: To study its biological activity,crude OPs were extracted and purified,and three dosages were applied in various methods such as mouse spleen lymphocytes transformation test(LTT),delayed type hypersensitivity(DTH)reaction,test of plaque forming cell,NK cell activity assay,assay of phagocytic function of the phagocyte,tumor-bearing mouse spleen lymphocyte activity test and assay of erythrocytic SOD activity.
     方法:采用热碱法提取,以三个剂量组(2.5、5、10mg/m l)进行正常小鼠脾淋巴细胞转化实验、迟发型超敏反应(DTH)、自然杀伤细胞(NK)活性测定,抗体形成细胞活性溶血空斑形成细胞实验(PFC)、碳廓清实验、腹腔巨噬细胞吞噬鸡红细胞实验、荷瘤小鼠脾淋巴细胞活性测定(MTT法)、红细胞超氧化物歧化酶(SOD)活性测定。
短句来源
  “plaque forming cell”译为未确定词的双语例句
     Results In 250mg/kg and 1 000mg/kg dose group,the thymus and spleen weight,the plaque forming cell count,the serum hemolysin volume,the toe thickness as well as the carbon clearance index were all significantly lower than those of control group(P<0 05,P<0 01).
     结果  2 5 0mg/kg、10 0 0mg/kg剂量组的胸腺和脾脏质量明显低于对照组 (P <0 0 1) ,PFC对数值、血清溶血素抗体积数、足跖肿胀度及碳廓清指数均低于对照组 (P <0 0 5 ,P <0 0 1)。
短句来源
     Then the mice were executed to break out the spleen suspension. The level of hemolysin and plaque forming cell were observed by using the spectrophotometer UV-2000 (λ =413 nm).
     处死小鼠后,取脾细胞混悬液,取上清液于UV-2000型分光光度计413nm处比色。
短句来源
     METHODS Plaque forming cell assay(PFC), TdR incorporation assay, enzyme linked immuno sorbed assay (ELISA)and enzyme linked immunospot assay(ELIspot).
     方法 溶血空斑实验 (PFC)、[3H] TdR参入、酶联免疫吸附实验 (ELISA)及酶联免疫斑点 (ELIspot)等方法。
短句来源
     Then the mice were executed to break out the spleen suspension. The level of hemolysin and plaque forming cell were observed by using the spectrophotometer UV-2000 (λ=413 nm).
     处死小鼠后,取脾细胞混悬液,取上清液于UV-2000型分光光度计413nm处比色,检测溶血空斑形成情况。
短句来源
     Injecting iv Lentinan (KS-2) has greatly increased the Plaque Forming Cell (PFC) ofmice. At the dosage of 100 mg/kg, This effect was the most prominent. it also potentiated the Delayed Type Hypersensitivity (DTH) reaction of footpad induced by BSA in normal mice.
     香菇多糖(KS—2)腹腔注射能明显增强小鼠脾抗体分泌细胞数(PFC),最佳给药剂量为100mg/kg,也可明显增强小鼠对BSA诱导的迟发型超敏反应性(DTH)。
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  plaque forming cell
Aged C57BL/10 and C57BL/10.BR mice demonstrated significantly reduced plaque forming cell (PFC) responses to both T-dependent and-independent antigens.
      
CMI was evaluated by measuring delayed type of hypersensitivity (DTH) response and humoral by plaque forming cell (PFC) assay.
      
Soybean inhibitor, aprotinin, α-antitrypsin and ovomucoid (1 mg) diminished significantly the direct plaque forming cell response per spleen 3 days after immunization with 108 sheep erythrocytes (SRBC), when given at the same time as the antigen.
      
The splenic plaque forming cell (PFC) response to red sheep cells were measured for treatment levels of 0.01 to 1,000 ppb (μg/kg).
      
Using a reverse plaque forming cell (PFC) assay the production of immunoglobulin (Ig) by peripheral blood mononuclear cells (MNCs) in vitro was studied in 12 patients with Wegener's granulomatosis (WG).
      
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A modified method of Jerne's assay was used to determine haemolytic plaque-forming cells (PFC) in mouse spleen. By means of this method we measured the dynamic changes of the immune status in tumor-bearing mice. The tumors used were solid Sarcoma 180 maintained in Tian-jin albino mice, four ascitic tumors including Sarcoma 180 maintained in Tian-jin albino and 615 strain mice, and Hepatoma and Ehrlich carcinoma maintained in C57BL mice. The results. showed that spleen PFC decreased as the tumors...

A modified method of Jerne's assay was used to determine haemolytic plaque-forming cells (PFC) in mouse spleen. By means of this method we measured the dynamic changes of the immune status in tumor-bearing mice. The tumors used were solid Sarcoma 180 maintained in Tian-jin albino mice, four ascitic tumors including Sarcoma 180 maintained in Tian-jin albino and 615 strain mice, and Hepatoma and Ehrlich carcinoma maintained in C57BL mice. The results. showed that spleen PFC decreased as the tumors progressed, and was hardly detected when the tumors were far advanced. The tumor-bearing mice commonly had striking splenomegaly with. decrease in number of small lymphocytes and. increase of 'immature' plasma cells, which might explain the poor immune responses of tumor-bearing mice.

本文应用改良的Jerne氏方法测定小鼠脾脏溶血空斑形成细胞。用此方法测定一株实体瘤(在津白小鼠上移植之肉瘤180)和四株腹水瘤(津白小鼠上移植之肉瘤180,615小鼠上移植之肉瘤180,以及C57BL小鼠上移植之肝癌和艾氏癌)带瘤动物免疫状态的动态变化。结果表明,随着肿瘤进行性生长,小鼠脾脏溶血空斑形成细胞逐渐减少,到晚期几乎不能测得。带瘤小鼠的脾脏普遍增大,病理组织学观察提示带瘤小鼠溶血空斑反应之低下与其脾脏的病理学改变相关。

It has been shown by haemolytic plaque-forming cell assay that there is an immunosuppressive factor(s) with high activity in the cell-free aseitic fluid of Sarcoma 130 maintained in 615 strain mice. The suppression is dose-dependent and becomes apparent only when the aseitic fluid is administered before immunization with sheep red blood cells. The immunosuppressive factor (s) seems partially heat-labile. The serum of the same tumor-bearing mice also possesses a strong immunosuppressive...

It has been shown by haemolytic plaque-forming cell assay that there is an immunosuppressive factor(s) with high activity in the cell-free aseitic fluid of Sarcoma 130 maintained in 615 strain mice. The suppression is dose-dependent and becomes apparent only when the aseitic fluid is administered before immunization with sheep red blood cells. The immunosuppressive factor (s) seems partially heat-labile. The serum of the same tumor-bearing mice also possesses a strong immunosuppressive activity. Furthermore, similar immunosuppressions were observed in normal recipient mice treated with cell-free aseitic fluids and sera of mice bearing some other strains of tumor including Hepatoma and Ehrlich carcinoma maintained in C57BL mice and Sarcoma 180 maintained in Tian-jin albino mice. Histopathologic changes of spleens induced by the cell-free aseitic fluid resembled those observed in spleens of tumor-bearing mice.

用溶血空斑试验证明,在615小鼠上移植的肉瘤180的无细胞腹水液中存在着高活性的免疫抑制因子。腹水液的免疫抑制作用与剂量相关,且仅在绵羊红血球抗原致敏前注射才能显示。此免疫抑制因子具有部分热不稳定的性质。肉瘤180带瘤小鼠的血清也有强免疫抑制活性。在C57BL小鼠上移植的肝癌和艾氏癌,在津白Ⅰ号小鼠上移植的另一株肉瘤180的腹水液和带瘤动物血清也有类似的免疫抑制作用。肿瘤无细胞腹水液引起的小鼠脾脏病理组织学变化与带瘤动物脾脏所见相类似。

The spleen cells taken from Balb/ cJ mice 3 days after immunization with PDRBC were fused with NS-1 myelo-ma cells by two different procedures in the presence of PEG.The hybridcells secreting the specific antibody were selected out in the HAT medium by means of the spot test of the culture fluid.(Table 1).The antibody secre-ting hybrid cells were then cloned by ...

The spleen cells taken from Balb/ cJ mice 3 days after immunization with PDRBC were fused with NS-1 myelo-ma cells by two different procedures in the presence of PEG.The hybridcells secreting the specific antibody were selected out in the HAT medium by means of the spot test of the culture fluid.(Table 1).The antibody secre-ting hybrid cells were then cloned by either limiting dilution or micromani-pulation methods and a cell line thus obtained was designated as CBH-1.After ten months of culture in vitro up to 69 generations,the ability of this cell line to secret specific monoclonal antibody to PDRBC was not decreased. The CBH-1 cells were also ino-culated into the peritoneal cavity of Balb/cJ or Balb/cCr mice and could be kept in these mice for at least four transplantation generations.The ability of three different agents for inducing the ascitic fluid was compared.As a result,pristane was found to be superior to liquid paraffin and Arlacel A. Attempt was also made to cultureCBH-1 in serum-free medium instead of the conventional medium containing 20% newborn calf serum (NCS).When the serum-free medium was provided with 5γ transferrin per ml,the CBH-1 cells could be maintained just as well as that in DMEM+20% NCS within 15 days (Table 2) The proportion of plaque forming cells after the first cloning was found to be 97.5% and this was increased to 99.8% after second cloning (Table 3).This result implies that the cell popula-tion of the CBH-1 clone was very ho-mogeneous with respect to the secre-tion of the specific antibody to PDR-BC.

以北京鸭红血球为抗原免疫Balb/cJ小鼠,三天后取脾细胞与小鼠NS-1骨髓瘤细胞在PEG作用下,用两种方法进行细胞融合。只有淋巴细胞杂交瘤细胞能在一种选择性培液——HAT液中生长,具有分泌抗体活性的杂交瘤细胞,借助其上清液的斑点试验加以选择。对斑点试验阳性的杂交瘤细胞进行克隆化和扩大培养,得到能专一分泌抗北京鸭红血球单克隆抗体的淋巴细胞杂交瘤,定名为CBH-1。在体外经过69代培养,历时十月余,分泌能力仍未见衰减。体内可传4—5代,腹水中可检出大量分泌抗体。用降植烷、Arlacel A和石蜡油诱发动物的腹水,其量以降植烷最高、石蜡油次之。使用国产石蜡油作为诱发腹水试验似具有一定实用价值。加5γ转铁蛋白至每毫升不含牛血清的DMEM液中,在15天的培养期内,结果至少与加20%小牛血清的培液的结果相似。首次克隆化后的CBH-1细胞中,空斑形成细胞达97.5%,再次克隆化的细胞中,空斑形成细胞达99.8%,证明是均一的细胞群落。本文还就杂交瘤制备过程及细胞稳定性问题进行了讨论。

 
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