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plaque forming cell
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  抗体形成细胞
    The effects of cytarabine on the immune function were determined with plaque forming cell (PFC) assay, hemolysin CH50 test and antigen specific rosette forming cell (ARFC) assay.
    所用的方法有体外抗体形成细胞试验,溶血素CH50的测定和抗原特异性花环形成试验。
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  plaque forming cell
Aged C57BL/10 and C57BL/10.BR mice demonstrated significantly reduced plaque forming cell (PFC) responses to both T-dependent and-independent antigens.
      
CMI was evaluated by measuring delayed type of hypersensitivity (DTH) response and humoral by plaque forming cell (PFC) assay.
      
Soybean inhibitor, aprotinin, α-antitrypsin and ovomucoid (1 mg) diminished significantly the direct plaque forming cell response per spleen 3 days after immunization with 108 sheep erythrocytes (SRBC), when given at the same time as the antigen.
      
The splenic plaque forming cell (PFC) response to red sheep cells were measured for treatment levels of 0.01 to 1,000 ppb (μg/kg).
      
Using a reverse plaque forming cell (PFC) assay the production of immunoglobulin (Ig) by peripheral blood mononuclear cells (MNCs) in vitro was studied in 12 patients with Wegener's granulomatosis (WG).
      
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When BALB/C mice which had rejected the anti-DNP IgE producing hybridoma B63, were im-munized with DNP proteins, they produced much less anti-DNP antibodies than control (normai) mice. The anti-DNP plaque-forming cell (PFC) number was much less when spleen celis from mice im-munized with DNP protiens were treated with sera of mice which had rejected the hybridoma B55 (Hy. Rej. mice) than the number from the same spleen celis not treated with the sera. The sera of Hy. Rej. mice contained an...

When BALB/C mice which had rejected the anti-DNP IgE producing hybridoma B63, were im-munized with DNP proteins, they produced much less anti-DNP antibodies than control (normai) mice. The anti-DNP plaque-forming cell (PFC) number was much less when spleen celis from mice im-munized with DNP protiens were treated with sera of mice which had rejected the hybridoma B55 (Hy. Rej. mice) than the number from the same spleen celis not treated with the sera. The sera of Hy. Rej. mice contained an inhibitor which was absorbed and eluted from an anti-mouse Immunoglobulin column and also from a mouse anti-DNP IgG2a column. The inhibition of PFC was hapten-reversible. In Westem blotting the eluates from the anti-DNP IgG2a column reacted as well as an anti-idiotypic antibody to anti-DNP IgE B53. These results establish that the inhibitor in the sera of Hy. Rej. mice was an auto-anti idiotypic antibody of the type which mimices the epitope (DNP) of tbe im munizing antigen.

排斥杂交瘤B_(53)(分泌抗DNP IgE)的BALB/C小鼠(Hy·Rej·mice),注射DNP蛋白质抗原后,几乎不形成抗DNP抗体。用PFC抑制试验证明,该小鼠血清中含有抑制抗DNP PFC形成的物质,此物质能被抗小鼠Ig或小鼠抗DNP IgG_(2a)的免疫吸附柱吸收。DNP-EACA可逆转Hy·Rej小鼠血清对DNP PFC形成的抑制作用,免疫印染技术证明,抑制物的分子量及免疫学特性与抗DNP IgE的抗独特型抗体完全一致。说明抑制物的本质是自身抗DNP IgE的抗独特型抗体。

This paper deals with the problem of how cytarabine influences the immune function in BALB/C mice. The effects of cytarabine on the immune function were determined with plaque forming cell (PFC) assay, hemolysin CH50 test and antigen specific rosette forming cell (ARFC) assay. The results showed that the average levels of PFC, CH50 and ARFC in the experimental group were all markedly lower than that in the control group. After statistical treatment there was very visible difference between...

This paper deals with the problem of how cytarabine influences the immune function in BALB/C mice. The effects of cytarabine on the immune function were determined with plaque forming cell (PFC) assay, hemolysin CH50 test and antigen specific rosette forming cell (ARFC) assay. The results showed that the average levels of PFC, CH50 and ARFC in the experimental group were all markedly lower than that in the control group. After statistical treatment there was very visible difference between these two groups (P<0.001). It is indicated that cytarabine was a significant inhibiter to the immune function in BALB/C mice.

本文讨论了阿糖胞苷对BALB/C小鼠免疫功能的影响。所用的方法有体外抗体形成细胞试验,溶血素CH50的测定和抗原特异性花环形成试验。实验数据经统计学处理,3种试验的用药组均值都低于对照组(P<0.001),表明阿糖胞苷对BALB/C小鼠的免疫功能有显著的抑制作用。

Objective: To investigate whether nanoselenium(NS) can prevent lung tumorigenesis in Kunming mice induced by tobacco specific nitrosamine 4 (methylnitrosamino) 1 (3 pyridyl) 1 butanone(NNK). Methods: The model of NNK induced lung tumorigegesis in mice was established by a single dose of 500μ mol/kg intraperitoneally. The incidence of tumor and numbersof tumor nodes in the lung were observed 8 months after NNK treatment. The immune functions, such as phagocytosis of macrophage in the abdominal cavity, plaque...

Objective: To investigate whether nanoselenium(NS) can prevent lung tumorigenesis in Kunming mice induced by tobacco specific nitrosamine 4 (methylnitrosamino) 1 (3 pyridyl) 1 butanone(NNK). Methods: The model of NNK induced lung tumorigegesis in mice was established by a single dose of 500μ mol/kg intraperitoneally. The incidence of tumor and numbersof tumor nodes in the lung were observed 8 months after NNK treatment. The immune functions, such as phagocytosis of macrophage in the abdominal cavity, plaque forming cells and delayed type hypersensitivity etc. were examined at day 1, 3, 7, 15 and 30 following NNK injection. Results: Eight months after NNK treatment, the incidence of lung tumor in mice was 93.3% (28/30), and the overage tumor nodes were 5.075± 2.98 per lung. The tumor was adenocarcinoma ascertained by histopathological examination. The 0.5, 1.0 and 3.0 ppm of selenium in NS decreased the incidence of tumor formation to 69.2% (18/26), 56.6% (17/30), 46.7% (14/30) and reduced tumor nodes to 2.29± 2.23, 1.37± 2.47, 1.09± 1.31 per lung,respectively. The dysfunction of immune system in mice were observed during one month fouoming NNK administration, and were obviously ameliorated by NS. Conclusion: The NS can inhibit NNK induced lung tumorigenesis in mice with a better dose response relationship, and the mechanism may be related to immunoregulation of selenium.

目的:研究烟草特异亚硝胺 4甲基亚硝胺 1 (3吡啶 ) 1丁酮 [4 (methylnitrosamino) 1 (3 pyridyl) 1 butanone,NNK]诱发昆明小鼠发生肺癌及纳米硒对肺癌的防治作用。方法:用单次腹腔注射 NNK(500μ mol/kg)制成昆明小鼠肺癌模型,观察 8个月后肺癌发生率和肺肿瘤灶结节数,并进行病理诊断。在注射 NNK后第 1、 3、 7、 15和 30天测定腹腔巨噬细胞吞噬率、脾抗体生成细胞数和迟发型变态反应等免疫功能指标。结果:注射 NNK后 8个月,昆明小鼠肺癌发生率为 93.3% (28/30),肺肿瘤灶计数为 (5.075± 2.98)个 /肺,病理学诊断为腺癌。 NNK处理的小鼠分别饮用含硒 0.5、 1.0、和 3.0 ppm的纳米硒 (NS),肺癌发生率和肺肿瘤灶计数分别下降到 69.2% (18/26)、 56.6% (17/30)、 46.7% (14/30)和 (2.29± 2.23)、 (1.37± 2.47)、 (1.09± 1.31)个 /肺。在注射 NNK后的 1个月内,小鼠多种免疫功能出现不同程度的改变,而 NS对 NNK所致小鼠免疫...

目的:研究烟草特异亚硝胺 4甲基亚硝胺 1 (3吡啶 ) 1丁酮 [4 (methylnitrosamino) 1 (3 pyridyl) 1 butanone,NNK]诱发昆明小鼠发生肺癌及纳米硒对肺癌的防治作用。方法:用单次腹腔注射 NNK(500μ mol/kg)制成昆明小鼠肺癌模型,观察 8个月后肺癌发生率和肺肿瘤灶结节数,并进行病理诊断。在注射 NNK后第 1、 3、 7、 15和 30天测定腹腔巨噬细胞吞噬率、脾抗体生成细胞数和迟发型变态反应等免疫功能指标。结果:注射 NNK后 8个月,昆明小鼠肺癌发生率为 93.3% (28/30),肺肿瘤灶计数为 (5.075± 2.98)个 /肺,病理学诊断为腺癌。 NNK处理的小鼠分别饮用含硒 0.5、 1.0、和 3.0 ppm的纳米硒 (NS),肺癌发生率和肺肿瘤灶计数分别下降到 69.2% (18/26)、 56.6% (17/30)、 46.7% (14/30)和 (2.29± 2.23)、 (1.37± 2.47)、 (1.09± 1.31)个 /肺。在注射 NNK后的 1个月内,小鼠多种免疫功能出现不同程度的改变,而 NS对 NNK所致小鼠免疫功能失衡的调节作用明显。结论: NS对 NNK诱发小鼠肺癌有明显防治作用,并有较好的剂量效应关系,其机理可能与硒的免疫调节作用有关。

 
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