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horseradish peroxidase
相关语句
  辣根过氧化物酶
    Study on Several Systems Used for Chemiluminescent Determination of Horseradish Peroxidase
    用化学发光法测定辣根过氧化物酶几种体系的研究
短句来源
    Enzyme-Amplified Lanthanide Luminescence Spectrometry──Studies on Horseradish Peroxidase-Terbium-p-Hydroxybenzoic Acid System and Its Application to the Determination of Horseradish Peroxidase and Tuberculosis Antibody
    酶联放大镧系螯合物发光法──辣根过氧化物酶(HRP)-铽-对羟基苯甲酸体系的研究及用于HRP和结核抗体的分析
短句来源
    Effects of 6-α-glucosyl-β-cyclodextrin on thermodynamic stability of horseradish peroxidase
    6-α-葡糖基-β-环糊精对辣根过氧化物酶热力学稳定性的影响
短句来源
    Study on Interaction Ways between Tb~(3+)and Horseradish Peroxidase
    Tb~(3+)离子与植物辣根过氧化物酶的作用方式探讨
短句来源
    A voltammetric enzyme linked immunoassay based on the new system of m aminophenol(MAP) H 2O 2 horseradish peroxidase(HRP) has been developed and used for the detection of HRP and southern bean mosaic virus(SBMV).
    提出间氨基酚 ( MAP) -H2 O2 -辣根过氧化物酶 ( HRP)伏安酶联免疫分析新体系 ,并用于南方菜豆花叶病毒 ( SBMV)的测定 .
短句来源
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  “horseradish peroxidase”译为未确定词的双语例句
    Enhanced Effect of Enhancing Agents on the Oxidation Reaction Catalyzed by Mimetic Horseradish Peroxidase ——Fe TPPS 4 4 AAP Phenol H 2O 2 System
    增效试剂对过氧化物模拟酶催化显色体系的增效作用——Fe-TPPS_4-4-AAP-苯酚衍生物-H_2O_2体系
短句来源
    Kinetic study between 4-aminoantipyrine and phenol by horseradish peroxidase
    酶催化氧化苯酚与4-AAP偶合反应的研究
短句来源
    Study on the Membrane-based Osmotic Immunoassay Using Horseradish Peroxidase as Tracer for the Rapid Detection of Carbaryl
    膜载体酶标记渗透式快速检测痕量西维因残留方法的研究
短句来源
    Polymedsation of Phenols Catalyzed by Horseradish Peroxidase in Aqueous MiCelle
    酚类聚合物在水相胶束中的酶促合成
短句来源
    STUDY ON THE ENHANCED OXIDATION REACTION CATALYZED BY MIMETIC HORSERADISH PEROXIDASE
    过氧化物模拟酶催化显色体系的增效作用
短句来源
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  horseradish peroxidase
A novel amperometric hydrogen peroxide sensor was proposed by co-immobilizing new methylene blue (NMB) and Horseradish peroxidase (HRP) on glassy carbon electrode through covalent binding.
      
Optimized conditions such as the reaction format (sandwich or direct), the concentrations of diluted horseradish peroxidase (HRP)-E.
      
Various orders of sequential coimmobilization of superoxide dismutase (SOD), catalase, and horseradish peroxidase (HRP) were tested in order to prepare a multienzyme antioxidant complex of these enzymes.
      
Stabilization of Diluted Aqueous Solutions of Horseradish Peroxidase
      
Effects of pH, enzyme concentration, and various supplements on the catalytic activity, temperature stability, and secondary structure of horseradish peroxidase (HRP) were studied in diluted aqueous solutions.
      
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Insulin was labeled with horseradish peroxidase by sodium periodate oxidation method. Guinea pig antibody against pig insulin was purified by affinity chroma- tography. Quantitative detection of insulin in different stages of manufacturing was carried out with ELISA (Enzyme linked Immunosorbent Assay) using antige- nenzyme conjugate. The components other than insulin in the pancreas did not interfere the result in ELISA. When the biological effective units detected by ELISA were in a range of 50-800 μIU/ml,...

Insulin was labeled with horseradish peroxidase by sodium periodate oxidation method. Guinea pig antibody against pig insulin was purified by affinity chroma- tography. Quantitative detection of insulin in different stages of manufacturing was carried out with ELISA (Enzyme linked Immunosorbent Assay) using antige- nenzyme conjugate. The components other than insulin in the pancreas did not interfere the result in ELISA. When the biological effective units detected by ELISA were in a range of 50-800 μIU/ml, the deviations were about 10-20%. All the influenced factors, such as the time of antibody adsorption, incuba- tion, reaction of enzyme and non-specific adsorption were discussed.

用高碘酸钠氧化法将辣根过氧化物酶标记于胰岛素。用亲和层析法纯化豚鼠抗猪胰岛素抗体。以ELISA-抗原竞争法测定猪胰岛素生产过程中间品效价。胰脏中非胰岛素成分对测定无干扰。对于生物效价的符合程度与RIA相当。在50-800μIU/ml测量范围内,测量偏差为10-20%。还讨论了对测定有影响的若干因素,如抗体包被时间、孵育时间、酶反应时间及非特异性吸附等。

In order to check the fact if the radiation induced post deacti-vation are possessed by all the enzymes. in this work the radiation effects of horseradish peroxidase (HRP) and glucose oxidase (GOD) were investigated. It was foundthat in dilute aqueous solution the irradiated HRP has the post deactivationalso. The effects of absorbed dose, initial HRP concentration in solution, atmosphere,temperature and additives (three kinds of complex agents: EDTA, CDTA and D)on the post deactivation of HRP were investigated....

In order to check the fact if the radiation induced post deacti-vation are possessed by all the enzymes. in this work the radiation effects of horseradish peroxidase (HRP) and glucose oxidase (GOD) were investigated. It was foundthat in dilute aqueous solution the irradiated HRP has the post deactivationalso. The effects of absorbed dose, initial HRP concentration in solution, atmosphere,temperature and additives (three kinds of complex agents: EDTA, CDTA and D)on the post deactivation of HRP were investigated. The regularity of post deactiva-tion of HRP is similar with the catalase. Oxygen in enzyme samples is necessaryfor the post deactivation. 5×10~(-3)mol/l of the three additives could control the phe-nomenon efficiently. Of course, the radiation deactivation of HRP was given aswell. In the case of GOD the post decativation was not found, although it's radiationdeactivation is also serious. It means that the radiation induced post deactivation is not common phenomenon for all enzymes.

报道了在稀水溶液中辣根过氧化物酶的辐射失活,并发现有辐射后失活现象。氧是后失活的必要条件;水辐解的分子产物H_2O_2对诱发这一后失活现象起一定作用。微量络合剂EDTA、GDTA等对辣根过氧化酶的辐射失活与后失活有不同程度的保护作用。在稀水溶液中葡萄糖氧化酶的辐射失活亦很严重,但却未发现有后失活现象。

In this paper is reportedchemiluminescent photopaphic detect-ion(CPD) joint with dot-immunobindingassay(DIA) to detect antibody againsthuman cytomegalovirus (HCMV) in se-rum. The oxidant, chemiluminescenceenhancer, their amount and the bufferwere optimized.Nitrocellulose(NC) filterwas used as the solid support for dete-cting pure horseradish peroxidase(HRP)and HCMV-Ab, and X-ray film used torecord the ligh emission. The detectionlimit of HRP was in the range of 2x10~(-18) ~10~(17) mol. The results of CPD...

In this paper is reportedchemiluminescent photopaphic detect-ion(CPD) joint with dot-immunobindingassay(DIA) to detect antibody againsthuman cytomegalovirus (HCMV) in se-rum. The oxidant, chemiluminescenceenhancer, their amount and the bufferwere optimized.Nitrocellulose(NC) filterwas used as the solid support for dete-cting pure horseradish peroxidase(HRP)and HCMV-Ab, and X-ray film used torecord the ligh emission. The detectionlimit of HRP was in the range of 2x10~(-18) ~10~(17) mol. The results of CPD for57 samples were compared with those ofconventional ELISA, the rate of agree-ment of the two methods being 84.2%(48/57). Mean-while, the concentrationof HRP labeled antibody used in CPDwas only 1/1000 of that in ELISA.

本文报道用化学发光自显影技术(Chemiluminescent photographic detection,CPD)与点免疫测定(Dot-immunobinding assay,DIA)结合检测了人血中巨细胞病毒(HCMV)抗体。从氧化剂、发光增强剂、缓冲液种类优选及浓度优选找出了化学发光废物液的最佳配方。以硝酸纤维素膜(NC)为载体,用x-光胶片记录化学发光,检测辣根过氧化物酶(HRP),检测限2×10~(-15)~10~(17)mol。用NC以来心法检测HCMV-Ab,与常规ELISA法对比,57例样品中,定性判断阴阳性彼此符合48例,符合率为84.2%(48/57),但CPD法所用酶标抗体浓度较ELISA法小千倍。

 
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