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   horseradish peroxidase 在 临床医学 分类中 的翻译结果: 查询用时:0.171秒
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horseradish peroxidase
相关语句
  辣根过氧化物酶
    ESTABLISHMENT OF A METHOD FOR DETECTION of escherichia coli by enhanced chemiluminescence with horseradish peroxidase
    应用辣根过氧化物酶化学发光法检测毒素原性大肠杆菌方法的建立
短句来源
    When SM-AKP and Sigma (RZ:3) Horseradish peroxidase(HRP) were coupled to goat-antihuman IgG, the highest titer of the conjugates was 1:6400.However, when the SM-AKP conjugat was kept at -6℃ for helf a year, the titer decreased from 1:6400 to 1:1600.
    自产AKP与Sigma(RZ:3)辣根过氧化物酶(HRP)结合于羊抗人lgG时,两种结合物的最高滴度均为1:6400,但自产AKP结合物低温(—6℃)保存6个月,滴度由原始1:8400下降到1:1600。
短句来源
    The antibody to hepatitis delta virus (Anti-HD) from the serum positive for HBsAg and Anti-HD was fractionated with ion-exchange chromatography of DE-52 and labelled with horseradish peroxidase (HRP).
    取乙型肝炎患者Delta抗体(抗—HD)阳性血清,经硫酸铵盐析和DE—52柱层析,得到纯化的抗—HD抗体,用辣根过氧化物酶标记。
短句来源
    In this system,a complex of L-lactic dehydrogenase,lactate oxidase,horseradish peroxidase,NADH and 3,3',5,5'-tetramethylbenzidine were used to compose a cycling system to increase the signal for the assay of alkaline phosphatase.
    该系统采用L-乳酸脱氢酶、乳酸氧化酶、辣根过氧化物酶、NADH和3,3′,5,5′-四甲基联苯胺组成一个循环体系,用以放大碱性磷酸酶产生的信号。
短句来源
    A voltammetric enzyme linked immunoassay method based on the new system of o aminophenol ( OAP ) H 2O 2 horseradish peroxidase ( HRP ) has been developed and used for the determination of HRP and thyroxine(T 4).
    提出邻氨基酚(OAP)H2O2辣根过氧化物酶(HRP) 伏安酶联免疫分析新体系并用于人血清中总甲状腺素的测定。
短句来源
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  “horseradish peroxidase”译为未确定词的双语例句
    Detection of Anti-nuclear Antibody by Horseradish Peroxidase Anti-peroxidase complex(PAP)
    过氧化物酶-抗过氧化物酶复合物(PAP)检测血清抗核抗体
短句来源
    Application of Nucleocapside Protein of Hantaan Virus and its McAb Labeled with Horseradish Peroxidase in Preparing of IgM Capturing ELISA Assay
    汉坦病毒核蛋白及其单抗标记物在建立ELISA试剂中的应用
短句来源
    3, 3', 5 , 5 '-ttetramethylbenzidine sulphate(TMBs), a new substrate of horseradish peroxidase (HRP) was used in ELISA for detecting M.tuberc.
    通过实验室操作,介绍3,3,5,5,一四甲基联苯胺硫酸盐(TMBS)作为ELISA底物,应用于血清结核抗原的检测;
短句来源
    A 800-bp polynucleotide probe for heat-labile enterotoxin of Escherichia coli (ETEC-LT+)was directly conjugated with the activated horseradish peroxidase complexes (HRP-PBQ-PEI+-NH3+).
    利用活化酶标复合物(HRP-PBQ-PEI+-NH3+)直接标记毒素原性大肠杆菌不耐热肠毒素(ETEC-LT)基因片段(0.8kb)作探针。
短句来源
    the appropriate detecting condition of rSAG1 ELISA was that the concentration of coated rSAG1 must be 5μg/ml, the human serum should be 1∶100 diluted, and goat anti human IgG conjugated to horseradish peroxidase was in 1∶2000 dilution; the CV value for IgG positive and IgG negative human sera were 10.9% and 10.7% respectively;
    以该重组抗原建立的rSAG1 ELISA的最佳检测条件为 :重组抗原rSAG1的包被浓度为 5 μg/ml,血清稀释度 1∶10 0 ,酶标记的羊抗人IgG 1∶2 0 0 0稀释 ;
短句来源
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  horseradish peroxidase
A novel amperometric hydrogen peroxide sensor was proposed by co-immobilizing new methylene blue (NMB) and Horseradish peroxidase (HRP) on glassy carbon electrode through covalent binding.
      
Optimized conditions such as the reaction format (sandwich or direct), the concentrations of diluted horseradish peroxidase (HRP)-E.
      
Various orders of sequential coimmobilization of superoxide dismutase (SOD), catalase, and horseradish peroxidase (HRP) were tested in order to prepare a multienzyme antioxidant complex of these enzymes.
      
Stabilization of Diluted Aqueous Solutions of Horseradish Peroxidase
      
Effects of pH, enzyme concentration, and various supplements on the catalytic activity, temperature stability, and secondary structure of horseradish peroxidase (HRP) were studied in diluted aqueous solutions.
      
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The peroxidase labeled antibody was prepared by IgG fraction and horseradish peroxidase with a one-step and two-step ghtaraldehyde methods. The enzyme labeled antibodies were used for detection.of the intraeellular viral and rickettsial antigens. light microscopic examination of the neurons in paraffin section of the brain stem and hippoeampus of the infected rnouse showed that the imm~noreaetire Japanese B encephalitis viral antigen distributed in the perikaryon, axon and dentrit-like processes. In the...

The peroxidase labeled antibody was prepared by IgG fraction and horseradish peroxidase with a one-step and two-step ghtaraldehyde methods. The enzyme labeled antibodies were used for detection.of the intraeellular viral and rickettsial antigens. light microscopic examination of the neurons in paraffin section of the brain stem and hippoeampus of the infected rnouse showed that the imm~noreaetire Japanese B encephalitis viral antigen distributed in the perikaryon, axon and dentrit-like processes. In the hemolymph smear of the natural infected tick the spotted fever rickettsia revealed distinctimmunoperoxidase staining.

用不同方法提取的抗体lgG与辣根过氧化物酶(HRP)按戊二醛一步法和二步法制备过氧化物酶标记抗体,并用于检出细胞内病毒和立克次体抗原。日本乙型脑炎病毒感染小白鼠脑石蜡切片的酶标抗体染色光镜检查结果表明:病毒抗原分布在脑干和海马回神经元的胞体、轴突和树突样胞浆突起内。自然感染蜱血淋巴涂片中的斑点热立克次体经酶标抗体染色显示清晰的阳性反应。

The effect of various fixatives on the immunoglobulin immunological activity of antigenic determinant sites of intracellular immunoglobulin has until lately only been studied to a limited extent. The present investigation has studied the effect of different fixatives on the demonstration of intraeellular immunoglobulin (IgG) and tissue morphology in paraffin embedded lymph nodes. Samples of lymph node were obtained from syngeneic WAB/Not male rats carried with hepatoma D23/ 47 and operated upon under ether inhalation...

The effect of various fixatives on the immunoglobulin immunological activity of antigenic determinant sites of intracellular immunoglobulin has until lately only been studied to a limited extent. The present investigation has studied the effect of different fixatives on the demonstration of intraeellular immunoglobulin (IgG) and tissue morphology in paraffin embedded lymph nodes. Samples of lymph node were obtained from syngeneic WAB/Not male rats carried with hepatoma D23/ 47 and operated upon under ether inhalation anethesia.The right and left lumbar lymph nodes were fixed in fixatives including Bouin's fluid, B-5, modified Millonig's phosphatebuffered formalin, Zamboni's fixa- tive and paraformaldehyde. The tissues were dehydrated in ethanol, cleared in zylene and embedded in paraffin.Staining was performed by PAP methods. Rabbit ant-irat IgG was used as first antibody, swine anti-rabbit IgG as second antibody, and soluble complex of horseradish peroxidase (HRP)-rabbit anti-horseradish peroxidase (PAP)as third antibody. The slides were incubated for peroxidase activity in3,3′-d iaminobenzidine tetrahydrochloride (DAB) medium. The greatest number of positively staining cells was seen in the cortex of rat lymph nodes. Bouin's fluid produced good preservation of antigenicity of immunoglobulins,sharp demarcation of the staining reaction and adequate preservation of tissue morphology as well. But the other fixatives gave a less satifactory result with bad tissue morphology and/or intense background staining. The author suggests fixation in Bouin's fluid so as to obtain a result demonstrating large amount of stained immunoglobulin containing celts and well preserved tissue morphology on paraffin embedded material.

本文研究了不同固定液对于未标记抗体酶(PAP)技术显示石蜡包埋大鼠淋巴结切片中细胞内免疫球蛋白(IgG)和组织形态的作用。Bouin氏液对于IgG抗原性的保存为最好,染色界线清晰,组织形态保持完整。

This paper describes the method of labelling horseradish peroxidase on horse anti-human IgE (periodate coupling method), and the survey of total serum IgE level on 148 cases of healthy donors in Changsha, Hunan, China, by enzyme-linked immunosorbent assay (double antibody sandwich method). The arithmetic means of total serum IgE levels of youth and adults groups were 607.57 and 708.83IU/ml, and the geometric means were 227.52 and 261.22IU/ml respectively. This data was lower than that reported in Singapore...

This paper describes the method of labelling horseradish peroxidase on horse anti-human IgE (periodate coupling method), and the survey of total serum IgE level on 148 cases of healthy donors in Changsha, Hunan, China, by enzyme-linked immunosorbent assay (double antibody sandwich method). The arithmetic means of total serum IgE levels of youth and adults groups were 607.57 and 708.83IU/ml, and the geometric means were 227.52 and 261.22IU/ml respectively. This data was lower than that reported in Singapore and India. The data of 18 -19 year-old young adult group met well with that reported in Beiging, but the data of > 20 year-old adult group was higher.

用辣根过氧化物酶标记马抗人IgE抗体;用ELISA 双抗体夹心法检测长沙地区148名正常人血清总IgE水平。结果表明:青年组算术均值与几何均值分别为607.57与227.52单位/毫升;成年组分别为708.33与261.22单位/毫升。

 
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