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affinity     
相关语句
  亲和
    A Novel SDE-grafted Affinity Adsorbent Mimicked the Ligand Function of LDL Receptor
    一种接有仿LDL受体功能结构短肽SDE的LDL亲和吸附剂的研究
短句来源
    Identification of the Peptides that Bind Thymidylate Synthase RNA with High Affinity Using mRNA Display
    mRNA体外展示技术筛选胸苷酸合成酶RNA亲和肽的研究
短句来源
    THE ISOLATION OF cAMP-DEPENDENT PROTEIN KINASE FROM PIG HEART MUSCLE BY HISTONEAGAROSE AFFINITY CHROMATOGRAPHY
    组蛋白-亲和层析分离猪心cAMP激活的蛋白激酶
短句来源
    Purification of T4 RNA Ligase by Blue-Dextran Sepharose 4B Affinity Chromatography
    兰色葡聚糖琼脂糖亲和层析法纯化T_4 RNA连接酶
短句来源
    Studies on E-receptors II. Purification of E-receptors with Affinity Chromatography
    淋巴细胞E受体的研究 Ⅱ.亲和层析法纯化E受体的研究
短句来源
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  亲和力
    Design and selection of high affinity DNA ligands for mutant single-chain derivatives of the bacteriophage 434 repressor
    噬菌体434单链阻遏蛋白的高亲和力DNA结合序列的设计和`筛选
短句来源
    Research of Anti-hTNF-α Chimeric Antibody Affinity Maturation in Vitro
    人TNF-α嵌合抗体体外亲和力成熟作用研究
短句来源
    In Vitro Affinity Maturation of an Anti-TNFα Antibody
    抗TNFα抗体的体外亲和力成熟
短句来源
    CHEMICAL AFFINITY——ITS PAST AND PRESENT
    化学亲和力的过去和现在
短句来源
    Determining Specificity, Affinity and Sensitivity of Monoclonal Antibodies by Radioimmunoassay
    单克隆抗体特异性、亲和力及敏感度放射免疫分析
短句来源
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  亲和性
    The Affinity of L-asparaginase and PEG_2-L-asparaginase to Asparagine and Glutamine
    天冬酰胺酶及PEG_2-天冬酰胺酶对L-天冬酰胺、谷氨酰胺亲和性的研究
短句来源
    The Regulatory Effects of PMA, Anti-CDS、 PHA and A23187 on the Expressions of Tac Antigen and High Affinity IL-2 Receptor on Jurkat Cells
    PMA、抗CD3、PHA和A23187对Jurkat细胞表达Tac抗原和高亲和性IL-2受体的调变作用
短句来源
    Regulation of Follitropin Receptor Affinity And Adenylate Cyclase Activity on Plasma Membrane of Bovine Calf Testis by G Protein
    小牛睾丸中G蛋白对FSH受体亲和性和腺苷酸环化酶活性的调节
短句来源
    High affinity anti-TNFα human monoclonal antibodies directly selected from pooled phage display library
    从混合的噬菌体抗体库中直接筛选高亲和性的抗肿瘤坏死因子-α人源单克隆抗体
短句来源
    Affinity between Two Wild and One Cultivated Pleurotus Species
    侧耳属(Pleurotus)二个野生种与一个栽培种之间亲和性研究
短句来源
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  亲缘
    and ⑥ the mutual affinity between Microstrobos and Microcachrys is proved.
    ⑥赞同Microstrobos和Microcachrys两属亲缘密切的观点。
短句来源
    Characteristics and affinity of the flora of Xishuangbanna,SW China
    西双版纳植物区系的特点与亲缘
短句来源
    Characteristics and Affinity of Tropical Flora in Southern China
    中国南部热带植物区系的特点与亲缘(英文)
短句来源
    AN EARLY CAMBRIAN PROBLEMATIC ORGANISM(ANABARITES) AND ITS POSSIBLE AFFINITY
    寒武纪早期疑难生物类群阿纳巴管(Anabarites)亲缘性的探讨
短句来源
    This flora has close affinity with tropical flora, in which the tropi-cal distribution patterns of genera are 77% and the tropical distribution patterns 61. 95%.
    本区系具有明显的热带亲缘,热带分布属占77%,热带分布种占61.95%。
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      affinity
    3D-QSAR STUDIES ON SUBSTITUTED DIHYDROPYRIDINES FOR THEIR α1A-ADRENERGIC RECEPTOR BINDING AFFINITY
          
    Both these models predicted binding affinity of internal and external test set compounds including the enantiomers of compound no.
          
    For example, N1-(4-aminobenzenesulfonyl)-5-azaskatole (18; Ki = 41 nM) displayed an affinity comparable to N1-(4-aminobenzenesulfonyl)skatole.
          
    All compounds were characterized by binding affinity determination for 5-HT2A and 5-HT2C subtypes and antagonistic activity for 5-HT2B receptor in rat stomach fundus.
          
    None of the new compounds showed affinity for 5-HT2A and 5-HT2C subtypes, but some of them displayed antagonistic activity in rat stomach fundus at micromolar concentrations.
          
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    (1) In studying the quantum efficiencies of photophosphorylation and of the Hill reaction, it was found that at low light intensities the quantum efficiencies of photophosphorylation(either"cyclic" with PMS or vitamin K as cofactors or coupled with Fe(CN)_6≡reduction) were abnormally low, whereas that of the simultaneously measured Hill reaction remains constant(Tab. 1). This unusual"light intensity effect" is evidently not related to the election transport chain, but is only concerned with the mechanism of...

    (1) In studying the quantum efficiencies of photophosphorylation and of the Hill reaction, it was found that at low light intensities the quantum efficiencies of photophosphorylation(either"cyclic" with PMS or vitamin K as cofactors or coupled with Fe(CN)_6≡reduction) were abnormally low, whereas that of the simultaneously measured Hill reaction remains constant(Tab. 1). This unusual"light intensity effect" is evidently not related to the election transport chain, but is only concerned with the mechanism of ATP production.The addition of large amounts of non-radioactive ATP to the reaction mixture(Tab. 2) or the previous formation of some AT~(32)P by preillumination(Tab. 3) does not attenuate this"light intensity effect". It is therefore not due to the decomposition of the final reaction product, ATP, nor an artifact introduced by the use of the radioactive method of AT~(32)P determination, and consequently must be concerned with the intermediate steps of phosphorylation.(2) By increasing the intensity of illumination, this unusual"light intensity effect" gradually disappears and the rate of photophosphorylation becomes proportional to light energy(Tab. 4). At a lower temperature(5℃), it is less pronounced. When weak light is given in short flashes interposed with dark intervals instead of continuously, the production of ATP is greatly lowered or completely obliterated(Tab. 5). All these results serve to show that the"light intensity effect" is due to a dark, thermal reaction by which some intermediate(s) in the phosphorylation path is destroyed or side-tracked.The reaction is probably enzymic in nature as evidenced by its rate order and its response to temperature. That the"light intensity effect" becomes insignificant at higher light intensities, indicates that the amount of the enzyme is limited and easily saturated so as to be negligible when the concentration of the photophosphorylation intermediate(s) is high, i.e., under strong illumination. That flashing light with short dark periods can completely abolish ATP formation means that this enzyme has a high affinity for the intermediate.(3) By illuminating chloroplasts with Mg~(++) and PMS then adding immediately to Pi and ADP in the dark, ATP can still be formed. But if the illuminated suspension is allowed to stand in darkness for 5 seconds before adding Pi and ADP, no ATP can be detected(Tab. 7). There is, therefore, an intermediate(Z~*) formed in light which can react with Pi and ADP in darkness (Fig. 1). At saturating intensities, this intermediate shows a concentration of 20-40 mμmole/μmole chlorophyll, i.e., in molecular rates of 1:25-50. At room temperature, this intermediate quickly dissipates(Tab. 7 and Tab. 8), but it becomes more stable as the incubating temperature is lowered to 5℃(Tab. 8).(4) By illuminating a similar suspension in presence of Pi and letting it stand in darkness for 5 sec. before adding ADP, the ATP formed is about 1/2 of that obtained by the immediate addition of ADP (Tab. 7). This shows that a second intermediate is formed with P/ (Z~P) and subsequently reacts with ADP. Apparently, in the chloroplast, the second intermediate is more stable than the first one(Z~*).(5) From the above results, it is concluded that the"light intensity effect" of photophosphorylation is probably due to the destruction or removal of intermediates in the phosphorylation mechanism. At least 2 such intermediates are indicated, the one before the participation of Pi is less stable, and it(or its precursor) is therefore mainly responsible for the said effect. The relation of the proposed scheme to the results of other workers and in comparison with oxidative phosphorylation is discussed.

    (1)在不同光强度下研究叶綠体的光合磷酸化作用和希尔反应,发现当光弱到一定程度后,光合磷酸化的效率,不论是“循环”或是“偶联”的都显著降低,而同时测定的希尔反应的效率则不变。因此,这个“光强效应”为光合磷酸化所特有,显然不是发生在“电子传递系统”或氧化还原部分。(2)在作用液中加入非放射性的ATP或预先照光形成一些AT~32P,再进行实验,这个“光强效应”仍同样出现,证明这个效应不是由于最终产物(ATP)的分解,亦不是由于应用放射性~(32)P测定方法所造成的假象。(3)这个“光强效应”在光强增加到一定程度以上时,即逐渐消失;在较低的温度下则减轻;在闪光条件下则比在连续光下更加显著。这些结果指出,“光强效应”是由于中间产物的破坏或转向其他代谢途径。此作用是一个暗反应,可能是酶促的。酶量少,容易达到饱和,弱光下中间产物少,被它作用的比重就大,强光下中间产物多,被它作用的比重就小,所以“光强效应”只在弱光下显著。(4) 叶綠体加Mg~(++)及PMS照以饱和强光,然后立即(<0.1秒)在暗中加入Pi及ADP,仍有很多ATP形成,但如在暗中过5秒钟后再加Pi及ADP,则几乎完全没有ATP形成。这指出叶綠体照光后产生...

    (1)在不同光强度下研究叶綠体的光合磷酸化作用和希尔反应,发现当光弱到一定程度后,光合磷酸化的效率,不论是“循环”或是“偶联”的都显著降低,而同时测定的希尔反应的效率则不变。因此,这个“光强效应”为光合磷酸化所特有,显然不是发生在“电子传递系统”或氧化还原部分。(2)在作用液中加入非放射性的ATP或预先照光形成一些AT~32P,再进行实验,这个“光强效应”仍同样出现,证明这个效应不是由于最终产物(ATP)的分解,亦不是由于应用放射性~(32)P测定方法所造成的假象。(3)这个“光强效应”在光强增加到一定程度以上时,即逐渐消失;在较低的温度下则减轻;在闪光条件下则比在连续光下更加显著。这些结果指出,“光强效应”是由于中间产物的破坏或转向其他代谢途径。此作用是一个暗反应,可能是酶促的。酶量少,容易达到饱和,弱光下中间产物少,被它作用的比重就大,强光下中间产物多,被它作用的比重就小,所以“光强效应”只在弱光下显著。(4) 叶綠体加Mg~(++)及PMS照以饱和强光,然后立即(<0.1秒)在暗中加入Pi及ADP,仍有很多ATP形成,但如在暗中过5秒钟后再加Pi及ADP,则几乎完全没有ATP形成。这指出叶綠体照光后产生能与Pi结合的中间产物(Z~*),其饱和量约为20—40mμmole/μmole叶綠素。它在室温(20—25度)迅速破坏或转向其他代谢途径,5秒后已不存在,在低温(5度)则可维持数秒。(5) 同样制剂加Pi再照光,然后暗5秒再加ADP,则ATP的产量,比立即加ADP者只减少一半。指出上述的中间产物(Z~*)与Pi结合后形成第二个中间产物(Z~P)在叶綠体内比较稳定。“光强效应”可能主要是Z~*或以前的中间产物被破坏或转向其他用途所引起。

    Saline solutions of calf thymus DNA have been heated at 100℃ for different periods and the change in viscosity followed in comparison with the changes in its affinity for two basic dyes, methyl green and pyronin. Under our experimental conditions, it is less likely that a molecular degradation of DNA has taken place in heat denaturation, but such treatment brought about a decrease in the quantity of methyl green bound to DNA up to about 50%. It can be inferred, therefore, that the affinity of DNA...

    Saline solutions of calf thymus DNA have been heated at 100℃ for different periods and the change in viscosity followed in comparison with the changes in its affinity for two basic dyes, methyl green and pyronin. Under our experimental conditions, it is less likely that a molecular degradation of DNA has taken place in heat denaturation, but such treatment brought about a decrease in the quantity of methyl green bound to DNA up to about 50%. It can be inferred, therefore, that the affinity of DNA for methyl green depends, to a great extent, on the molecular spatial configuration of the former, or more concretely, on the double helix structure.The type of binding curves of methyl green by native and by some heat-denatured DNA's has been explored on the basis of a general theory of the interaction of a large polymeric molecule with small molecules.On summarizing all the results obtained a mechanism of binding of methyl green by DNA has been proposed.A competition has been observed between methyl green and pyronin in the double binding process by DNA, with the former predominating over the latter. From these results and from the difference in the chemical structures of these two dyes we may suggest that pyronin would not set up such strict claim to the molecular spatial configuration of DNA as in the case of methyl green.

    (1)小牛胸腺DNA溶液在100℃保温不同时间以后,测定了粘度的变化,同时观察并比较甲基绿与焦宁对于受热变性前后的DNA的结合能力的差异。(2)根据高分子物质与低分子物质结合作用的一般规律,测定了甲基绿对于天然态DNA及数种热变性DNA的结合数据。(3)论证了甲基绿对DNA的结合能力主要是与DNA分子的双螺旋空间构型的完整性有关。(4)对甲基绿与DNA结合的机制作了初步的讨论。(5)从甲基绿与焦宁对DNA的双重结合作用所得结果表明:两种染料能以不同程度同时与DNA结合,但在此两者之间存在着一定程度的竞争作用,以甲基绿占明显的优势。从所得结果以及两种染料的分子结构的差异可猜测,此两种染料对于DNA分子的空间构型的要求程度不同。

    The difference spectrum of a mixture of haematin and FMN at pH 7.4 measured against the two reactants separately has been found to show 3 distinct peaks at 525,610 and 685 mμ. Plot of the difference absorbancy at these 3 wavelengths according to the so-called continuous variation method~([7]) clearfly demonstrates the formation of 3 complexes with the proportions of haematin: FMN=1:1, 1:2 and 1:4 in the order of decreasing affinity between the reactants. By the same method, aged-haematin has been shown...

    The difference spectrum of a mixture of haematin and FMN at pH 7.4 measured against the two reactants separately has been found to show 3 distinct peaks at 525,610 and 685 mμ. Plot of the difference absorbancy at these 3 wavelengths according to the so-called continuous variation method~([7]) clearfly demonstrates the formation of 3 complexes with the proportions of haematin: FMN=1:1, 1:2 and 1:4 in the order of decreasing affinity between the reactants. By the same method, aged-haematin has been shown to combine with FMN to form an 1:1 complex with a maximum at 585 mμ in the difference spectrum. Spectrophotometric titration of a mixture of agedhaematin and FMN in the absence of air also suggests the formation of complexes with one or both of the reactants in the reduced form. It appears from theoretical considerations, the formation of the 1:4 complex of haematin and FMN and the effect of the ionic strength of the medium on the difference spectra that the interaction of haematin and FMN may be of the charge transfer type. The implication of these findings on our understanding of the electron transfer process in the respiratory chain has been briefly discussed.

    血红素与FMN反应后的差光谱在500mμ—800mμ范围内有三个差吸收峰,波长分别为525mμ,610mμ及685mμ。用所谓连续变易法作图结果表明反应产物包括:血红素比FMN等于1:1,1:2及1:4等三种络合物,结合紧密程度依次降低。老化血红素与FMN反应只能产生1:1的络合物,表现出一个差吸收峰(585mμ)在过量连二亚硫酸钠存在下差光谱形状略为不同,血红素及FMN皆为全还原型时,络合物络合能力降低一个数量级。在隔绝空气的条件下逐步加入还原剂可以观察到在二者未完全还原前可能尚有其他络合物的生成。无论血红素是否经过老化它与FMN络合生成的产物差光谱皆受溶液离子强度的影响。对血红素与FMN络合的物质进行了讨论,所生成的某些络合物很可能是属于所谓电荷转移形式。这两种生物氧化中重要辅基如生成电荷转移络合物,可能与呼吸链中的电子传递有密切关系。

     
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