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affinity chromatography     
相关语句
  亲和层析
     Studies on the Application of Affinity Chromatography Coupled with Bio-Mass Spectrometry in the Analysis of Protein Drugs in Vivo
     亲和层析与生物质谱联用技术在生物体内蛋白质药物分析中的应用研究
短句来源
     THE ISOLATION OF cAMP-DEPENDENT PROTEIN KINASE FROM PIG HEART MUSCLE BY HISTONEAGAROSE AFFINITY CHROMATOGRAPHY
     组蛋白-亲和层析分离猪心cAMP激活的蛋白激酶
短句来源
     Preparation and application of some common ligands in affinity chromatography
     几种共同配基亲和层析系统的制备和应用
短句来源
     THE ABILITY OF ELIMINATION OF DNA IN NAMALVA PREPARATION BY ANTIBODY AFFINITY CHROMATOGRAPHY
     抗体亲和层析纯化对Namalva干扰素制品中DNA的清除能力
短句来源
     Preliminary Investigation on the Affinity Chromatography of λ-phage
     λ噬菌体亲和层析的初步研究
短句来源
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  亲和色谱
     Purification of human platelet factor 4 by monoclonal antibody (SZ-95) affinity chromatography
     单克隆抗体SZ-95亲和色谱纯化人血小板第4因子
短句来源
     Chromatographic Behavior of Mouse Serum IgG in Protein G Perfusion Affinity Chromatography
     小鼠血清IgG的蛋白G灌注亲和色谱行为的研究
短句来源
     The purity of mAb was over 92% after protein A affinity chromatography.
     腹水mAb经Protein A亲和色谱柱纯化后,纯度达92%以上。
短句来源
     coli BL21(DE3),then induced by IPTG,and purified by affinity chromatography.
     coli BL21(DE3)表达菌株,利用IPTG诱导表达,并用亲和色谱纯化COMT。
短句来源
     GAD was purified from rice germ by ammonium sulfate fractionation, DEAE- Sephrose FF chromatography, Superdex 200 gel filtration, and Glu- Sephrose CL 4B affinity chromatography.
     通过(NH4)2SO4分级沉淀、DEAE-Sephrose FF离子交换色谱、Superdex 200 凝胶过滤色谱和Glu Sephrose CL 4B亲和色谱等分离纯化技术对米胚GAD进行了分离纯化,从米胚中分离得到的GAD经 SDS-PAGE鉴定为单一组分。
短句来源
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  亲和层析法
     Conclusion:CD26 can be purified to homogeneity by ADA-Sepharose 4B affinity chromatography.
     结论:用ADA-Sepharose 4B亲和层析法可纯化CD26达电泳纯。
短句来源
     Methods: Ni NTA affinity chromatography was established to extract the target recombinant proteins rOmpL1/1 and OmpL1/2,LipL32/1 and rLipL32/2,LipL41/1 and rLipL41/2 expressed by the different genotypes.
     方法 :建立 Ni- NTA亲和层析法 ,提取不同基因型表达的目的重组蛋白 r Omp L1/ 1和 Omp L1/ 2、L ip L32 / 1和 r Lip L32 / 2、Lip L4 1/ 1和 r Lip L4 1/ 2。
短句来源
     Routine genetic engineering technique was applied to construct the prokaryotic expression systems of genotypes ompL1/1 and ompL1/2, and Ni-NTA affinity chromatography was performed to extract the target recombinant products rOmpL1/1 and rOmpL1/2. Immune aurosol electron microscopy was selected to locate the position of OmpL1s on leptospiral envelope.
     采用常规基因工程技术构建ompL1/1和ompL1/2主要基因型原核表达系统,Ni-NTA亲和层析法提纯目的重组表达产物rOmpL1/1和rOmpL1/2。 采用胶体金免疫电镜技术,对OmpL1s进行膜定位。
短句来源
     Purification of Porcine Plasma a_2-Macroglobulin by Affinity Chromatography
     亲和层析法分离纯化猪血浆α_2-巨球蛋白
短句来源
     SDS-PAGE and BioRad Agarose Image Pattern Analysis System were applied to examine the rHla expression. Ni-NTA affinity chromatography was performed to collect rH1a.
     采用SDS-PAGE和BioRad凝胶图象分析系统检查rH1a表达情况,Ni-NTA亲和层析法收集rH1a。
短句来源
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  亲和色谱法
     After nucleosides were extracted from urine on phenyl boronic acid affinity chromatography, the analysis was performed on a column (46 mm id×250 mm, 5 μm) at 22 ℃ using a linear gradient elution comprising 25 mmol/L KH2PO4 solution (pH 455) and 60% methanol in water with UV detection at 260 nm.
     通过苯基硼酸亲和色谱法提取尿中核苷,在色谱柱(4 6mmi d ×250mm,5μm)上以25mmol/L磷酸二氢钾溶液(pH4 55)和60%的甲醇水溶液作为流动相进行二元梯度淋洗,于22℃下进行反相色谱分离,260nm处紫外检测。
短句来源
     Methods A combination of euglobulin precipitation, ion-exchange chromatography, (NH4)2SO4 precipitation and affinity chromatography was used in the process of purification.
     方法采用优球蛋白沉淀、离子交换色谱、(NH4)2SO4沉淀和亲和色谱法进行纯化。
短句来源
     AFFINITY CHROMATOGRAPHY FOR PURIFICATION OF Cu,Zn-SOD
     亲和色谱法纯化Cu,Zn-SOD
短句来源
     coli BL21(DE3)and purified by histidine affinity chromatography. P9-ZFD antibody was prepared through immunizing Balb/c rats with purified P9-ZFD protein.
     coliBL2 1(DE3)中胞内诱导表达P9 ZFD蛋白 ,组氨酸亲和色谱法纯化P9 ZFD蛋白并免疫Balb/c小鼠制备其多克隆抗体。
短句来源
     Separation of αAmino Acids and Peptides by Chelated Metal Ion Affinity Chromatography
     螯合金属离子亲和色谱法分离α-氨基酸和肽
短句来源
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  affinity chromatography
After Ni2+-NTA resin affinity chromatography, the purity of the recombinant T800/T1300 protein was more than 90%.
      
After colony screening, the bacterium was cultured and the product was purified with affinity chromatography.
      
The enzymes were purified by affinity chromatography on an α-cyclodextrin polymer and gel filtration on Biogel P-150 and proved to be electrophoretically homogeneous.
      
A protein that inhibited the proteolytic activity of trypsin was isolated from amaranth leaves (Amaranthus cruentus) by affinity chromatography on trypsin-Sepharose.
      
Sequential isolation of biologically active compounds by metal-chelate affinity chromatography followed by azoadsorbent affinity chromatography allowed us to obtain highly purified products.
      
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A study has been made on the preparation of the trypsin inhibitor from insulin production wastes be affinity chromatography on insolubilized trypsin columns and by the salting-out procedure with ammonium sulfate. Some of the properties of the trypsin inhibitor and its method of identification have been described.

本文报道了用固相酶亲和层析技术和硫酸铵盐析法,从提取胰岛素后的残渣和废液中, 提取猪胰蛋白酶抑制剂的两种方法,并简要介绍了猪胰蛋白酶抑制剂的性质的鉴定方法。

Cyclic AMP-dependent protein kinase has been isolated from pig heart muscle by affinity chromatography. The affinity absorbents were synthesized by coupling the whole histone of calf thymus with agarose 4B through the active esters of spacers of different length. The small peptides were used as the spacers to avoid the nonspecific hydrophobic absorption of the enzyme. The affinity absorbent with glycylglycine as side-arm gave the best result in the isolation of protein kinase among those with...

Cyclic AMP-dependent protein kinase has been isolated from pig heart muscle by affinity chromatography. The affinity absorbents were synthesized by coupling the whole histone of calf thymus with agarose 4B through the active esters of spacers of different length. The small peptides were used as the spacers to avoid the nonspecific hydrophobic absorption of the enzyme. The affinity absorbent with glycylglycine as side-arm gave the best result in the isolation of protein kinase among those with sidearms of alanine, glyoylglycine and leucylglycylglycine.

本文报导用亲和层析分离猪心蛋白激酶的结果,以及对这个酶的性质的初步观察。亲和吸附剂的配基为全组蛋白,它与琼脂糖4B或直接相联;或分别通过丙氨酸、甘氨酰甘氨酸、亮氨酰甘氨酰甘氨酸三种不同长度“手臂”的活化酯,在温和的条件下相联。上述四种亲和吸附剂的分离效果以双甘肽为“手臂”的最好。猪心粗抽液经亲和层析一步分离得到的蛋白激酶,其比活提高五十倍左右,活力回收达百分之五十。初步测定了这个酶的分子量、等电点、对ATP的K_m和cAMP的结合常数,观察到它对cAMP可能有两种结合部位。活力保存实验表明,酶在甘油中4℃保存四个月活力不变,cAMP激活性质也不变。

Rat liver mRNA coding for serum albumin has been isolated and purified to apparent homogeneity on gel elec-trophoresis by means of polysome im-munoprecipitation and poly (U)-Sep-harose affinity chromatography.Theisolation of albumin-synthesizing poly-some involved the incubation of liverpolysome with an antibody (the firstantibody) against native albumin wh-ich was.obtained from rabbit an-tiserum by the use of affinity chroma-tography on columns of albumin-Seph-arose.The binding is highly specificand...

Rat liver mRNA coding for serum albumin has been isolated and purified to apparent homogeneity on gel elec-trophoresis by means of polysome im-munoprecipitation and poly (U)-Sep-harose affinity chromatography.Theisolation of albumin-synthesizing poly-some involved the incubation of liverpolysome with an antibody (the firstantibody) against native albumin wh-ich was.obtained from rabbit an-tiserum by the use of affinity chroma-tography on columns of albumin-Seph-arose.The binding is highly specificand occurs through an immunologicalrecognition of nascent albumin peptidechains on the polyribosomes.This firstantibody reaction is followed by theincubation of the polysome-antibodycomplex with a second antibody (sheepanti-rabbit γ G).The polysomedou-ble antibody complex is then sedimen-ted through a discontinuous sucrosegradient to remove unreacted polysomeand unreacted antibody.The albuminpolysomal RNA was extracted fromthe polysome immunoprecipitate byphenol-chloroform-isoamyl alcohol (50∶48∶2).Rat lver cell albumin mRNA is purified on poly (U)-Sepharose chromatography to remove rRNA and tRNA.The isolated albumin mRNA mi-grated as a pure species during elec-trophoresis on gel of 2% polyacryla-mide-0.5% agarose.Upon addition ofthe purified mRNA to a cell-free pro-tein-synthesizing system derived fromwheat germ,the total translationproduct was identified as authentic al-bumin by means of double antibodyimmunoprecipitation and gel electrophoresis on which about 70-80% nascentalbumin peptide was migrating alongwith native albumin.The translation activity in wheat germ systemunder optimal conditions indicatesthat the albumin mRNA was purifiedas high as 53fold than the originalalbumin polysomal RNA.

采用双抗体技术和Poly(U)-Sepha-rose 亲和层析法,分离和纯化出为均一的大鼠肝细胞白蛋白mRNA。把酸乙醇—硫酸铵分部和Sephadex G-150柱层析得到均一的大鼠血清白蛋白,免疫家兔得到抗血清,上白蛋白-Sepharose 柱,得到了纯的抗白蛋白抗体。用它与肝聚核糖体一起温育,得到合成白蛋白的聚核糖体。它们之间的结合是高度专一的,似乎是通过核糖体上的初生白蛋白多肽的免疫识别作用所致。反应的免疫复合物用第二抗体追踪,然后把聚核糖体—第一抗体—第二抗体复合物,通过一个不连续的蔗糖梯度,除去未反应的聚核糖体和抗体。用苯酚—氯仿—异戊醇(50∶48∶2)从免疫沉淀中分离出白蛋白聚核糖体RNA,上poly(U)-Sepharose 柱,得到大鼠肝细胞白蛋白mRNA。所分离白蛋白mRNA,在2%聚丙烯酰胺—0.5%琼脂糖凝胶电泳时为一条带。在依赖于mRNA 的麦胚无细胞蛋白合成系统中,所有的翻译产物由第一抗体识别,第二抗体追踪,用凝胶电泳法鉴定为真正的白蛋白——约有70—80%新生白蛋白多肽与天然白蛋白一起移动。麦胚抽提物的翻译分析表明,提纯的白蛋白mRNA 的活力可高达白蛋白pRNA 的53倍。

 
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