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soft rot
相关语句
  软腐病
    Construction of Genetic Modified Pseudomonas fluorescens Which Habouring aiiA Gene from Bacillus thuringiensis and Its Activity Against Soft Rot Disease
    含苏云金芽孢杆菌aiiA基因的重组荧光假单胞菌的构建及对软腐病的抗病活性
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  “soft rot”译为未确定词的双语例句
    Pscherichia coli DHS (pCPP430) E430 which well expresses the harpin gene from Erwinia amylovora has been tested for its capacity of inducing hypersensitive response (HR) and inducing plant disease resistance, for example, that to tomato's early leaf blight, to rice's blast, to corn's Northern and Southern leaf blight, and to potato's soft rot.
    用表达harpin基因的大肠杆菌DHS进行了诱导植物抗病性的研究,发现表达harpin基因的重组菌除可明显诱发烟草、番茄叶片的过敏反应外,对玉米、水稻叶片也可诱发过敏反应,反应比相应阳性对照病菌速度快,坏死斑也更典型。
短句来源
    But in the weaker compatible interaction, the production of active oxygen species after 8 hours became obvious and reached a peak at 20 hour, which enhanced host resistance to soft rot disease.
    但在弱亲和互作中,互作8 小时后,即有明显的活性氧的释放,20 小时达到高峰,并且这种活性氧的释放提高寄主的抗病性。
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  soft rot
The review deals with transformation of natural and synthetic aromatic compounds by fungi (causative agents of white rot, brown rot, and soft rot, as well as soil filamentous fungi).
      
A laboratory test method against soft rot is modified in a way that it is also applicable in Kolle flasks.
      
Then, with the help of the laboratory test, the effects of some essential constituents of wood preservatives against soft rot are compared.
      
Moreover, details are given on the susceptibility of some wood species and the destructive power of different soft rot fungi.
      
Delayed drying improved the soft rot protection compared with normal drying and treatment with CCA.
      
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Three soft rot erwinias were different in growth and the production ofextracellular enzymes at 21,26 and 31℃ and in 6 liquid media.Ecc growsmost rapidly at 26℃,Eca at 21℃,while growth of Ech was similar in 3temperatures.Comparatively,the growth and pectinlyase(PL) activity of Echare the highest and its polygalacturonase (PG) activity the lowest among 3bacteria.AS temperature increased,PL activity of 3 bacteria decreased,proteaseof Ech sharply reduced,but protease of Eca significantly increased.There is...

Three soft rot erwinias were different in growth and the production ofextracellular enzymes at 21,26 and 31℃ and in 6 liquid media.Ecc growsmost rapidly at 26℃,Eca at 21℃,while growth of Ech was similar in 3temperatures.Comparatively,the growth and pectinlyase(PL) activity of Echare the highest and its polygalacturonase (PG) activity the lowest among 3bacteria.AS temperature increased,PL activity of 3 bacteria decreased,proteaseof Ech sharply reduced,but protease of Eca significantly increased.There is asignificant relation between PL activity in cell-free filtrates of culture andtheir maceration to potato tuber tissues.However,in filtrate There is noor little pectinase activity,but protease activity is regular and also maceratestuber tissues.

3种软腐细菌在21,26和31℃下及6种培养液中的生长量和产生胞外酶的能力各不相同。Ecc在26℃生长最好,Eca为21℃,Ech在三种温度下的生长量差别不大。与其它2种细菌比较,Ech的生长及产生果胶裂解酶(PL)的能力总是最强的。随着温度的升高,各种细菌的PL活性都下降。果胶水解酶(PG)变化不大,但Ech在高温下产PG有减少趋势,且总没有另二种细菌的活性强。Eca以26℃时产PG最适宜。Ech在较高温度下蛋白酶活性大大下降,而Ecc,变化不大,Eca的蛋白酶活性则随温度升高而加强。培养液的无细胞滤液对马铃薯块茎组织的浸离力与其中PL的活性有密切关系,但没有或只有很低果胶酶活性,而蛋白酶活性正常的这种滤液也能使小薯碟浸离。Eca在31℃培养后果胶酶活性很低,蛋白酶活性很高,其无细胞滤液对马铃薯组织也表现较强浸离力。

On CPG and similar media supplemented with TTC(2,3,5—triphenyl tetra-zolium chloride),the naturally occured colonial mutant strains of Erwinia caro-tovora pv.carotovora Dye(EccM),isolated from soft rot potato tubers,showed white and small colonies,compared with large and red ones of the common wild strains(EccW).This difference can not be used as an indicator to represent their differences in dehydrogenase activity because they had similar activities when determined with cell suspensions washed from the...

On CPG and similar media supplemented with TTC(2,3,5—triphenyl tetra-zolium chloride),the naturally occured colonial mutant strains of Erwinia caro-tovora pv.carotovora Dye(EccM),isolated from soft rot potato tubers,showed white and small colonies,compared with large and red ones of the common wild strains(EccW).This difference can not be used as an indicator to represent their differences in dehydrogenase activity because they had similar activities when determined with cell suspensions washed from the same medium.The white and small form of EccM on CPG+TTC plate is a false negative reaction for their dehydrogenase activity.This is resulted from the following facts:1)EccM strains produced much more acid than EccW when grown on medium with glucose,suc-rose,fructose or related sugars as carbon source,their acid production ability co-uld be visualized by the clear zone around colony on CPG+CaCO_3 plates;2)the buffering capacity of CPG medium was very weak,only 1/8—1/10 of that of K′SB;the pH value in the medium soon reduced to less than 6 after growing of the bacteria;3)under such acidic conditions,the growth of the bacteria was severely

在含0.005%氯化三苯基四氮唑(TTC)的酪蛋白氨基酸—蛋白胨—葡萄糖(CPG)平板上,胡萝卜软腐欧氏杆菌苗落变异型菌株(简称EccM)生长差、菌落既小且白、而普通野生型菌株(Eccw)则为红而又大的菌落。EccM的苗落特征与下列因素有关:①当以葡萄糖、果糖、蔗糖或其它相关的糖类为碳源时,EccM菌株产生的酸特别多,若培养基中加入碳酸钙、菌落周围可形成酸溶的透明圈;②CPG培养基的缓冲力很弱,只有KingsB的1/8—1/10,EccM生长后、培养基pH很快降到5.5以下,这是生长的pH下限值;③在pH<6时,菌体脱氢酶活性受到强烈抑制,还原TTC的能力明显减弱。上述因素共同作用就使EccM在TTC平板上生长差,颜色白,与EccW很容易区别开。

The soft rot bacteria (ECC) were grown in the liquid MS medium containing different carbon sources and compared for the cell wall degration enzymes produced in the culture supernatants. The extracellular pectate lyase and polygalacturonase were not detected but extracellular proteolytic enzyme activity was detected in the supernatants of the same medium. Extracellular proteases produced by StEcc-12 were purified by ammonium sulfate fractionation and diethylaminoethyl cellulose column chromatography (DEAE-52)....

The soft rot bacteria (ECC) were grown in the liquid MS medium containing different carbon sources and compared for the cell wall degration enzymes produced in the culture supernatants. The extracellular pectate lyase and polygalacturonase were not detected but extracellular proteolytic enzyme activity was detected in the supernatants of the same medium. Extracellular proteases produced by StEcc-12 were purified by ammonium sulfate fractionation and diethylaminoethyl cellulose column chromatography (DEAE-52). lsoelectric focusing tests showed that the extracellular protease had two kinds of isoenzymes with isoelectric points of 8.3 and 8.9 respectively.The denatured temperature of the protease is about 50℃.The pH changing did not obviously effect on activity of the protease. Soft-rot was evident when potato tubers were treated by purified proteases.The sections from the tissue treated by purified proteases showed that the cell walls obviously degrated.Transposon Tn5 originated from E.coli 1830/pJB4J1::Tn5 was inserted into wild type strain (StEcc-12) by conjugation.11 mutants which protease activity was defective were obtained from 2000 transconjugates. These mutants did not liquefy gelatin or hydrolyte casein. The ability of these mutants to macerate potato tissue was obviously lower than the ability of wild type. Isoelectric focusing maps of mutants was apparent different from the maps of wild type strain. The results suggest that protease of Erwinia carotovora subsp. carotovora should be in cooperation with pectinase in the process of soft-rot of potato tuber tissues.

胡罗卜软腐欧氏杆菌(Erwinia carotovora var.carotovora Dyc)在合成培养基MS中产生两种蛋白酶,但在无细胞滤液中测不到果胶裂解酶和果胶水解酶的活性。用等电聚焦电泳测得这两种蛋白酶都是碱性蛋白酶,等电点分别为8.3和8.9。变性温度为49—50℃,对酸或碱具有较高的稳定性。纯化的蛋白酶单独作用马铃薯块、能使其变软,切片观察表明细胞壁被明显降解。通过滤膜结合法,将供体菌1830/pJB4J1::Tn5的转座子转入到野生型StEcc—12中,从2000个接合子中获得了11个缺少产蛋白酶能力的突变体。这些突变体的分泌性蛋白质等电聚焦图谱与野生型菌株明显不同,其中一个变突体蛋白酶带完全缺失,另外一个则出现8条新的蛋白带,其他5个属部分缺失。这些突变体对马铃薯块组织离析、腐烂的能力都有不同程度的减弱。研究结果认为在由胡罗卜软腐欧氏杆菌引起的软腐病程中蛋白酶和果胶酶是协同作用的,果胶酶降解植物细胞间和胞壁中的果胶质,而蛋白酶则降解植物细胞壁和膜中的蛋白质。

 
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