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      triphosphate
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  三磷酸
     2PCR: The 1/10 volume of reaction solution (7 μL) was obtained and 200 μmol/L 2'- deoxyribonucleoside triphosphate (dNTP), 50 pmol/L oligonucleotide primer and 0.5 μLα-32PdCTP (Beijing Furui Company), 1×Buffer, 2.5 U TaqDNA polymerase were added into the solution.
     ②聚合酶链反应:取1/10体积穴7μL雪反转录液,分别加入200μmol/L2’-脱氧核苷三磷酸,50pmol/L寡聚核苷酸引物,0.5μL[α-32P]dCTP穴北京福瑞公司雪,1×Buffer,2.5UTaqDNA聚合酶。
短句来源
     Methods: The electrochemical behavior of p-aminophenol at nucleoside triphosphate,single chain DNA(ssDNA) and double chain DNA(dsDNA) modified electrode in 0.05mol/L Na_2HPO_4-0.05 mol/L NaH_2PO_4-0.10 mol/L NaCl buffer solution(pH=7.30) were studied in this paper.
     方法:在0.05 mol/L Na2HPO4-0.05 mol/L NaH2PO4-0.10mol/L NaC l缓冲体系(pH=7.30)中研究对氨基苯酚在核苷三磷酸修饰电极及DNA修饰电极上的电化学行为。
短句来源
     The stability constants of the ternary mixed ligand complexes Ca(HRB)(UTP) n- (HRB=Phen, Bpy and Trp;UTP=uridine 5′ triphosphate; n= 2 or 3)have been determined by potentiometric pH titration in aqueous solution (I=0.1 mol/L, KNO 3;25 ℃).
     用pH电位滴定法测定了Ca(Ⅱ )与尿苷 5′ 三磷酸 (UTP)和芳香杂环碱HRB形成的三元混配配合物Ca(HRB) (UTP) n - (HRB =Phen ,Bpy和Trp ;n =2或 3)的稳定常数 (I =0 .1mol/L ,KNO3;2 5℃ ) .
短句来源
     Synthesis of first strand cDNA: 2 μg total RNA was got at 65 ℃, degeneration for 3 minutes, admixture of 10×Buffer, 25 mmol/L magnesium chloride, 10 mmol/L 2'- deoxyribonucleoside triphosphate (dNTP) as well as oligonucleotide primer, RNA enzyme inhibitor (RNasin) and reverse transcriptase were added in order. Volume of total reaction was 20 μL, and it was put at 42 ℃ for heat preservation for 90 minutes.
     第一链cDNA合成:取2μg总RNA于65℃变性3min,依次加入10×Buffer,25mmol/L氯化镁,10mmol/L2’-脱氧核苷三磷酸混合物,寡聚核苷酸引物和RNA酶抑制剂穴RNasin雪与反转录酶,总反应体积20μL,置42℃保温90min。
短句来源
     Methods The electrochemical behaviors of p-chlorophenol were determined at nucleoside triphosphate, single chain DNA (ssDNA) and double chain DNA (dsDNA) modified electrode in 0.05 mol/L Na2HPO4 -0.05 mol/L NaH2PO4 -0.10 mol/L NaCl buffer solution (pH=7.30).
     方法在0.05mol/LNa2HPO4-0.05mol/LNaH2PO4-0.10mol/LNaCl缓冲体系中(pH=7.30),研究对氯苯酚在核苷三磷酸修饰电极及DNA修饰电极上的电化学行为。
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  三磷酸盐
     Nelarabine(Arranon),a T-cell selective nucleoside analogue,is the water-soluble prodrug of 9-beta-D-arabinofuranosyl guanine(ara-G). Ara-G is converted into ara-G triphosphate(ara-GTP) inside leukemic blasts by phosphorylation,leading arrest of DNA synthesis and cell apoptosis.
     奈拉滨为T细胞选择性核苷类似物,为9-β-D-阿糖呋喃糖鸟嘌呤(ara-G)的水溶性前体药物,ara-G在白血病的原始细胞中转化为ara-G的三磷酸盐(ara-GTP),从而抑制DNA合成,导致细胞死亡。
短句来源
     This hypothesis was tested using N,N-dipropyl-5-carboxamidotryptamine (N,N-DP-5-CT)-stimulated guanosine-5'-O-(3-thio)triphosphate (GTPγS) binding assessed by autoradiography.
     使用对氮 ,氮 -二丙基 -5 -氨基色胺刺激的鸟苷 -5 '-氧-( 3-硫代 )三磷酸盐 ( [35S]GTPγS)结合体进行放射自显影照相的方法评估 ,测定了该假设。
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  “triphosphate”译为未确定词的双语例句
     Activity change of RBC membrance Ca~(2+)-Mg~(2+)adenosine triphosphate in hypertension
     红细胞Ca~(2+)-Mg~(2+)-ATP酶活性变化与高血压病的关系初探
短句来源
     Triphosphate of β-L-D4A is degraded at a lower rate in 2.2.15 cells, and it may have a longer half-life.
     β-L-D4A三磷酸化物在2.2.15细胞内降解缓慢。
短句来源
     Results: The average recovery rates of myocardial adenosine triphosphate (ATP), adenosine diphosphate(ADP)and adenosine monophosphate (AMP) were 99%-107%, 96%-104% and 95%-119%, respectively;
     结果:心肌三磷酸腺苷(ATP)、二磷酸腺苷(ADP)和一磷酸腺苷(AMP)平均回收率分别为 99% ~107%, 96% ~104%和 95% ~119%;
短句来源
     Results The sensitivity of adnosine triphosphate stress ~(99m) Tc-MIBI myocardical imaging on diagnosis of CHD was 93.8%(60/64),specificity was 84.2%(10/19),coincidence was 91.5%(76/83).
     结果ATP负荷试验99mTc-MIBI心肌显像诊断冠心病敏感性为93.8%(60/64),特异性为84.2%(16/19),符合率为91.5%(76/83);
短句来源
     Under respiration gating, the largest detection error of metabolites within five volunteers was phosphomonoesters ( PME ) 39. 5% , inorganic phosphate ( Pi ) 40. 4% , phosphodiesters (PDE) 23.2%, adenosine triphosphate; γ-ATP24. 3%,α-ATP20. 1 % ,βb-ATP 24. 9% , respectively.
     在呼吸门控触发扫描采集下,5名志愿者中代谢物检测的最大误差分别为:PME 39.5%、Pi 40.4%、PDE 23.2%、γ-ATP 24.3%、α-ATP 20.1%、β-ATP 24.9%。
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  triphosphate
A new theory of bio-energy transport along protein molecules, where energy is released by the hydrolysis of adenosine triphosphate (ATP), has recently been proposed for some physical and biological reasons.
例句来源      
The levels of malondialdehyde (MAD), total superoxide dismutase (SOD), glutathione (GSH), adenosine triphosphate (ATP) and serum amylase (SAM) were measured.
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Hemostaseological description of the heparin-adenosine triphosphate (ATP) complex
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Antidiabetic properties of high molecular weight heparin-adenosine triphosphate complex
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Repeated intramuscular administration of the heparin-adenosine triphosphate (ATP) complex or ATP increased plasma anticoagulant and fibrinolytic activities and depressed the anticoagulation system in rats at the age of 10-11 months.
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         As reported by Borst and Slater, dinitrophenol (DNP), the uncoupling agent, can under certain conditions cause an inhibition of the oxidation of succinate by intact mitochondria. We have studied this effect of DNP on succinate oxidation by respirationcontrolled rat-liver mitochondria with a vibrating platinum microelectrode and found that, in absence of added inorganic orthophosphate and adenosine diphosphate (ADP), the action of DNP is closely related to its concentrations. When DNP is added in low concent...
            (1)DNP对于琥珀酸氧化的影响随浓度不同而异,低浓度时,呼吸受激活,其程度随浓度升高而增強,不出現抑制現象。高浓度(30μM以上)DNP只引起短时間氧化激活,随即引起抑制,随浓度升高,抑制出現愈早,氧化激活愈小,預先加入Amytal足以防止上述抑制現象,在抑制出現后加入Amytal亦能使琥珀酸氧化部分恢复。(2)砷酸盐激活的琥珀酸氧化仅在有Amytal的条件下,才出現抑制現象。(3)ATP对上述两种情况引起的琥珀酸氧化抑制都具有一定的解除作用。(4)就实驗結果所做分析支持琥珀酸氧化需要能量激活的看法。
文摘来源
         As a direct consequence of the interdigitating filament model of the myofibril and the corresponding theory of muscular contraction put forward by H. E. Huxley and A. F. Huxley, it is evident that the muscle would lose its ability to contract if its sarcomere had been stretched to a length greater than the sum of the lengths of primary and secondary filaments. For the honey bee striated muscle, the sum of the lengths of the two sets of filament would be about 5.4μ, on the basis of the interdigitating filame...
            本文报告我們观察拉长的蜜蜂間接飞翔肌肌原纤維收縮能力的結果。在肌小节长度超过8微米以后,三磷酸腺苷仍能引起肌原紆維的縮短,而且在肌小节拉长后出現的結构变化也是可逆的。这些結果不能在H.E.Huxley和A.F.Huxley提出的肌原紆維由两組蛋白細絲构成的模型和收縮的細絲滑行理論的基础上来解释。
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         This paper presents the results of experiments concerning the influence of salt concentration, adenosine triphosphate, pH value, alcohol and urea on the binding of Congo Red with the glycerinated rabbit psoas muscle and toad sartorious muscle fibers. Within a certain range of concentrations, salt promotes the binding of Congo Red with the muscle fibers and impedes the washing out of Congo Red from stained muscle fibers in solutions of similar electrolytic composition without Congo Red. Adenosine tr...
            本文报告一些外界条件对剛果紅与甘油抽提的骨骼肌肌纤維結合的影响。所用方法大体上可以分为两类:(1)将肌纤維束浸于不同的剛果紅溶液中,观察染色的速率和深度有何差別;(2)将已經染色的肌纤維束浸于其他成分和用来染色的剛果紅溶液相同,但是不含剛果紅的洗脫液中,过一定时間后改变洗脫液的成分,观察由此所引起的洗脫速率的变化。得到的結果主要如下: (一)在一定范圍內增加盐濃度能促进剛果紅和肌纤維的結合,也能减低剛果紅从已經染色的肌纤維洗脫的速率,甚至使已被洗脫的剛果紅又重新和肌纤維結合。 (二)在洗脫液不含盐或含盐較少的情况下,三磷酸腺苷的作用和其它盐类的相仿,可是在洗脫液盐濃度較高的情况下,它却能增加洗脫的速率。 (三)两类实驗結果都表示硷性溶液(pH 9—10)不利于剛果紅和肌纤維結合,而且在硷性剛果紅溶液中染色的肌纤維或已經染色的肌纤維用硷性溶液处理后都基本上不出現二向色性。 (四)甲醇和乙醇能可逆地阻止剛果紅和肌纤維結合。 (五)脲能增加剛果紅从已經染色的肌纤維中洗脫的速率。文中还討論了剛果紅分子和收縮蛋白分子結合方式的問題,认为盐鍵和氫鍵大概都在起作用,而且在肌纤維中排列有規則的一部分剛果紅分子可能是通过氫鍵和...
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