2PCR: The 1/10 volume of reaction solution (7 μL) was obtained and 200 μmol/L 2'- deoxyribonucleoside triphosphate (dNTP), 50 pmol/L oligonucleotide primer and 0.5 μLα-32PdCTP (Beijing Furui Company), 1×Buffer, 2.5 U TaqDNA polymerase were added into the solution.
Methods: The electrochemical behavior of p-aminophenol at nucleoside triphosphate,single chain DNA(ssDNA) and double chain DNA(dsDNA) modified electrode in 0.05mol/L Na_2HPO_4-0.05 mol/L NaH_2PO_4-0.10 mol/L NaCl buffer solution(pH=7.30) were studied in this paper.
The stability constants of the ternary mixed ligand complexes Ca(HRB)(UTP) n- (HRB=Phen, Bpy and Trp;UTP=uridine 5′ triphosphate; n= 2 or 3)have been determined by potentiometric pH titration in aqueous solution (I=0.1 mol/L, KNO 3;25 ℃).
Synthesis of first strand cDNA: 2 μg total RNA was got at 65 ℃, degeneration for 3 minutes, admixture of 10×Buffer, 25 mmol/L magnesium chloride, 10 mmol/L 2'- deoxyribonucleoside triphosphate (dNTP) as well as oligonucleotide primer, RNA enzyme inhibitor (RNasin) and reverse transcriptase were added in order. Volume of total reaction was 20 μL, and it was put at 42 ℃ for heat preservation for 90 minutes.
Methods The electrochemical behaviors of p-chlorophenol were determined at nucleoside triphosphate, single chain DNA (ssDNA) and double chain DNA (dsDNA) modified electrode in 0.05 mol/L Na2HPO4 -0.05 mol/L NaH2PO4 -0.10 mol/L NaCl buffer solution (pH=7.30).
Nelarabine(Arranon),a T-cell selective nucleoside analogue,is the water-soluble prodrug of 9-beta-D-arabinofuranosyl guanine(ara-G). Ara-G is converted into ara-G triphosphate(ara-GTP) inside leukemic blasts by phosphorylation,leading arrest of DNA synthesis and cell apoptosis.
This hypothesis was tested using N,N-dipropyl-5-carboxamidotryptamine (N,N-DP-5-CT)-stimulated guanosine-5'-O-(3-thio)triphosphate (GTPγS) binding assessed by autoradiography.
Results: The average recovery rates of myocardial adenosine triphosphate (ATP), adenosine diphosphate(ADP)and adenosine monophosphate (AMP) were 99%-107%, 96%-104% and 95%-119%, respectively;
Results The sensitivity of adnosine triphosphate stress ~(99m) Tc-MIBI myocardical imaging on diagnosis of CHD was 93.8%(60/64),specificity was 84.2%(10/19),coincidence was 91.5%(76/83).
A new theory of bio-energy transport along protein molecules, where energy is released by the hydrolysis of adenosine triphosphate (ATP), has recently been proposed for some physical and biological reasons.
The levels of malondialdehyde (MAD), total superoxide dismutase (SOD), glutathione (GSH), adenosine triphosphate (ATP) and serum amylase (SAM) were measured.
Repeated intramuscular administration of the heparin-adenosine triphosphate (ATP) complex or ATP increased plasma anticoagulant and fibrinolytic activities and depressed the anticoagulation system in rats at the age of 10-11 months.
As reported by Borst and Slater, dinitrophenol (DNP), the uncoupling agent, can under certain conditions cause an inhibition of the oxidation of succinate by intact mitochondria. We have studied this effect of DNP on succinate oxidation by respirationcontrolled rat-liver mitochondria with a vibrating platinum microelectrode and found that, in absence of added inorganic orthophosphate and adenosine diphosphate (ADP), the action of DNP is closely related to its concentrations. When DNP is added in low concent...
As a direct consequence of the interdigitating filament model of the myofibril and the corresponding theory of muscular contraction put forward by H. E. Huxley and A. F. Huxley, it is evident that the muscle would lose its ability to contract if its sarcomere had been stretched to a length greater than the sum of the lengths of primary and secondary filaments. For the honey bee striated muscle, the sum of the lengths of the two sets of filament would be about 5.4μ, on the basis of the interdigitating filame...
This paper presents the results of experiments concerning the influence of salt concentration, adenosine triphosphate, pH value, alcohol and urea on the binding of Congo Red with the glycerinated rabbit psoas muscle and toad sartorious muscle fibers. Within a certain range of concentrations, salt promotes the binding of Congo Red with the muscle fibers and impedes the washing out of Congo Red from stained muscle fibers in solutions of similar electrolytic composition without Congo Red. Adenosine tr...