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  condyloma
Perianal giant condyloma acuminatum: A case report
      
A correlative analysis of cervical lesions in patients with vulva condyloma acuminatum
      
OBJECTIVE To analyze the relation ship among vulva condyloma acuminatum, high-risk human papillomavirus (HPV) infections and cervical epithelium lesions.
      
Human papillomaviruses cause anorectal condyloma and anal cancer.
      
The expression of Fas/FasL in peripheral blood lymphocytes and the level of IL-2 in serum of patients with condyloma acuminata
      
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ABC staining of enzyme immunoassay were used to assay specific antigen of human papilloma virus(HPV)in tissue samples collected from 65 cases of cervical ,squamou: carcinoma, and 23 cases of proliferative verrucous vaginal diseases (14 casea of condyloma acuminata, 9 cases of papilloma).The findings showed positive antigen reactions to HPV is 13.84%(9/65) of cervical squamous carcinama, and 52.17%(12/23)in the group of patients with proliferative verrucous vaginal disseases lespectively. The positive antigen...

ABC staining of enzyme immunoassay were used to assay specific antigen of human papilloma virus(HPV)in tissue samples collected from 65 cases of cervical ,squamou: carcinoma, and 23 cases of proliferative verrucous vaginal diseases (14 casea of condyloma acuminata, 9 cases of papilloma).The findings showed positive antigen reactions to HPV is 13.84%(9/65) of cervical squamous carcinama, and 52.17%(12/23)in the group of patients with proliferative verrucous vaginal disseases lespectively. The positive antigen reactions to HPV could be shown in three types: 1.pantonucleus type: whole nucleus showed homogeneous dark brown, 2.intranucleus cytorrhycte type:heterogeneous brown cytorrhyctes seen in the nucleus, 3.paranuclear granule type:small brown granules occurred in the paranuclear space of koilocyte.

本文用ABC免疫酶染色法对65例宫颈鳞癌、23例女阴疣状增生病(尖锐湿疣14例、乳头状瘤9例)的组织标本,进行了人乳头瘤病毒(HPV)属特异性抗原的检测。在宫颈鳞癌及女阴疣状增生病中HPV抗原的阳性率分别为13.84%(9/65)和52.17%(12/23)。HPV抗原阳性反应的表现有三种类型:(1)全核型:细胞核呈均匀深棕色反应;(2)核内包涵体样型:核内出现棕色不规则形的包涵体样结构;(3)核周颗粒型:在挖空细胞的核周空隙区内出现较细小的棕色颗粒。

The HPV DNA related sequences, the expression of HPV capside antigen and relationship between the ultrastructural feature of lesion and diverse types of HPV infection in 35 cases of the cervical pr61iferative lesions(cervical condyloma, cervical cancer)were analyzed on the levels of cell,subcell and molecule by using nucleic acid hybridization, immunohistochemical method and electron microscopic technique. The results showed: (1) Moot of cervical condyloma was related to HPV 11; cervical cancer was...

The HPV DNA related sequences, the expression of HPV capside antigen and relationship between the ultrastructural feature of lesion and diverse types of HPV infection in 35 cases of the cervical pr61iferative lesions(cervical condyloma, cervical cancer)were analyzed on the levels of cell,subcell and molecule by using nucleic acid hybridization, immunohistochemical method and electron microscopic technique. The results showed: (1) Moot of cervical condyloma was related to HPV 11; cervical cancer was mainly related to HPV 16. (2) The expression of HPV capside antigen presented negatine relation to malignant degree of lesion in the cervical proliferative lesion, the higher the malignant degree of lesion was, the lower the expression of HPV capside antigen was. (3) HPV 11 induced lesion characterized by a high degree of koilocytoois as a marker and HPV 16 appeared to preferentially induce lesion with marked nuclear atypia in the cervical benign proliferative lesion (cervical condyloma). (4) The expression of HPV capside antigen was much higher in the cervical benign proliferative lesion with koilocytosis caused by HPV 11, on the contray,the expression of HPV capside antigen was much lower in the cervical benign proliferative lesion with marked nuclear atypia caused by HPV 16.

本研究分别用核酸杂交、免疫组织化学和电子显微镜术等方法从细胞、亚细胞和分子水平上比较分析了35例子宫颈增殖性病变中人乳头瘤病毒DNA相关序列,人乳头瘤病毒衣壳抗原的表达以及病变超微结构变化特征与不同型别人乳头瘤病毒感染之间的关系。结果表明:(1)宫颈湿疣多与人乳头瘤病毒11型(HPV—11)相关,宫颈癌主要与人乳头瘤病毒16型(HPV—16)相关;(2)子宫颈增殖性病变中人乳头瘤病毒衣壳抗原的表达与病变的恶性程度呈负相关,病变恶性程度愈高,人乳头瘤病毒衣壳抗原的表达愈低;(3)宫颈湿疣中HPV—11以诱导细胞的重度凹空病变为标志,HPV—16以引起细胞核的非典型性改变为特征;(4)人乳头瘤病毒衣壳抗原的表达主要见于HPV—11感染的具有凹空病变的宫颈湿疣中,而在HPV—16感染引起细胞核非典型性改变为特征的宫颈湿疣中,人乳头瘤病毒衣壳抗原的表达较低。以上结果表明:HPV—16致癌潜能较强。

The Physical state of 32 cases of cervical proliferative lesions which were positive to dot blot hybridization of HPV—16DNA were further analyzed by using labelled HPV—16 DNA probe, restriction enzyme technique and Southern blot hybridization method. The results showed that HPV—16 DNA sequences could only be detected as an episome in normal cervical epithelia and cervical benign proliferative lesions(cervical condyloma),on the contray, HPV—16 DNA was found to have been integrated into the genome of host...

The Physical state of 32 cases of cervical proliferative lesions which were positive to dot blot hybridization of HPV—16DNA were further analyzed by using labelled HPV—16 DNA probe, restriction enzyme technique and Southern blot hybridization method. The results showed that HPV—16 DNA sequences could only be detected as an episome in normal cervical epithelia and cervical benign proliferative lesions(cervical condyloma),on the contray, HPV—16 DNA was found to have been integrated into the genome of host cell in of cervical malignant proliferative lesions(cervical cancer) while in the cervical malignant lesions(cervical intraepithelial neoplasia, CIN), HPV—16 DNA sequences coexisted in form of episome and integration. These results suggest that integration of HPV—16 genome in cervical epithelia may play an important role in the process of occurrence and development of cervical cancer.

本研究以a—~(32)P—dCTP标记的HPV—16DNA为探针,应用限制性内切酶技术和Southern印迹杂交方法进一步分析了32例HPV—16DNA斑点杂交阳性的宫颈活检标本中HPV—16基因组的物理状态。结果表明:正常宫颈上皮和宫颈良性增殖性病变(宫颈湿疣)中,HPV—16DNA均以染色体外游离基因的形式存在;宫颈癌前病变(宫颈上皮内新生物)中,HPV—16DNA以游离和整合两种状态共存;宫颈恶性增殖性病变(宫颈癌)中,HPV—16DNA完全以整合的形式存在于宿主细胞基因组中。以上结果提示:宫颈上皮中HPV—16基因组的整合在宫颈癌的发生发展过程中可能起着重要作用。

 
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