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tetracycline resistance
相关语句
  四环素抗性
     Bacteria were reisolated from the diseased plants caused by T2123 and T2124.Tetracycline resistance testing and plasmid analysis indicated that all bacterial cells contained pGX1252,implying that pGX1252 was stable in T2016 and T2017 without antibiotics selective pressure in plant.
     从侵染T2123、T2124的病株中重新分离病菌,四环素抗性检测和质粒检测表明所有细菌均含有pGX1252,说明在植物体内无抗生素选择压力情况下pGX1252能在T2016、T2017中稳定存在。
短句来源
     Screening of high expression clone of heterologous gene by using tetracycline resistance genotype
     四环素抗性表型筛选外源基因高效表达克隆
短句来源
     A Km~rTc~n recom-binant plasmid was also obtained by Pvu Ⅱ digest from the plasmid pAL32 andpUB110. Based on the inactivation of the tetracycline resistance of these recombi-nants, it is suggested that EcoRⅤ and Pvu Ⅱ sites are located on Tc~r gene of thePlasmid pAL32.
     用pUB110与pAL32在PvuⅡ位点连接后的重组质粒也为Km~rTc~s。 根据这些重组子四环素抗性的失活,推知pAL32上EcoRV与PvuⅡ切点均位于其四环素抗性基因上。
短句来源
     Methods An O139 strain, BJ30 which lysogenic CTXФ genome was marked with tetracycline resistance gene was induced with mitomycin C a DNA-damaging agent. The phage particles were used to infect the classical standard strains 569B and O395. The obtained tetracycline resistant transductants were further identified for transduced CTXФ genome.
     方法 用DNA破坏剂丝裂霉素C诱导噬菌体CTXΦ基因组被四环素抗性基因标记的O139菌株BJ30 ,继而体外转染古典型标准菌株 5 6 9B和O395 ,对获得的四环素抗性的转导菌株进行转染噬菌体基因组的鉴定。
短句来源
     The HindIII fragments of Artacus ricini chromosomal DNA,which worked as promoter for the tetracycline resistance gene in the promoter cloning plasrnid pHE5 of Escherichia coli,were studied.
     利用大肠杆菌启动子克隆载体pHE5,研究了蓖麻蚕染色体的Hind Ⅲ酶解片段在大肠杆菌中作为四环素抗性基因启动子的功能作用。
短句来源
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  “tetracycline resistance”译为未确定词的双语例句
     (3)Tetracycline sensibility 9.7%( n=3),tetracycline resistance 90.3%( n=28),tetM 90.3%( n=28);
     (3)四环素敏感株9.7%(n=3),耐药株80.6%(n=25),中介株9.7%(n=3),四环素不敏感率90.3%,检出tetM基因株90.3%(n=28);
短句来源
     Conclusion The results indicated that the 7.4 kb plasmid might mediate high level penicillin resistance,while the 42.5 kb and 39.5 kb plasmids might have relationship with tetracycline resistance.
     而非PPNG或TRNG中所携带的质粒谱型则相对复杂。 结论7.4kb的质粒可能会介导淋球菌高水平的青霉素耐药性,42.5kb及39.5kb质粒可能与介导四环素高水平耐药有关。
短句来源
     All erythromycin resistant isolates had ermB and/or mefA gene of these strains, 79.5% had ermB , 17.8% had both ermB and mefA , and 2.7% had mefA of the 185 isolates, 87% was positive for the tetracycline resistance gene tetM .
     所有红霉素耐药株均检出ermB和或mefA,其中79.5%为ermB阳性,17.8%为ermB和mefA同时阳性,2.7%为mefA阳性。
短句来源
     Study on Detection of Tetracycline Resistance Gene(tetC) of Pathogenic Salmonella from Swine by PCR and Nucleic Acid Probe
     PCR和核酸探针检测猪源沙门氏菌四环素耐药基因tetC的研究
短句来源
     Detection of tetracycline resistance tet M gene of isolated streptococcus pneumoniae strains in Suzhou area
     苏州地区肺炎链球菌分离株tet M基因检测
短句来源
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  相似匹配句对
     Detection of the Tetracycline Resistance Gene from E. coli by PCR
     大肠杆菌耐四环素基因的检测
短句来源
     Advanced research in the bacterial resistance mechanisms of tetracycline
     细菌对四环素类抗生素的耐药机制研究进展
短句来源
     ASIPIRIN RESISTANCE
     阿司匹林抵抗
短句来源
     Aspirin Resistance
     阿司匹林抵抗现象
短句来源
     Polarographic Determination of Tetracycline
     四环素的极谱法测定
短句来源
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  tetracycline resistance
Strain AX101 lacks the tetracycline resistance gene that was a common feature of other recombinant Zm constructs.
      
We describe a cloning vector, designated pCTBtet, carrying a tetracycline resistance gene (TetR) between the leader peptide and mature CTB.
      
The transposon-containing streptococcal plasmids pAM211, pCF10, and pINY1275 have been transferred at high frequency (10-2-10-3 per recipient, selecting for tetracycline resistance) to the Gram-positive anaerobe Clostridium acetobutylicum.
      
The soil bacterium Azotobacter vinelandii was genetically transformed by chromosomal integration to ampicillin and/or tetracycline resistance using restriction endonuclease-linearized plasmids.
      
Inactivation of the putative tetracycline resistance gene HP1165 in Helicobacter pylori led to loss of inducible tetracycline re
      
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The lambda plac5 EcoR1 DNA fragment containing the lac operator, promoter and most of the structural gene for β-galactosidase was linked to the plasmid pBR322, creating a plasmid designated pMG4. Analysis With restriction endonucleases indicates that the lac operon and the ampicillin resistance gene in pMG4 are transcribed in the same direction and the Hind Ⅲ site located in the lambda DNA region of the lac insert is near the other site in the tetracycline resistance gene of pBR322. A plasmid, designated...

The lambda plac5 EcoR1 DNA fragment containing the lac operator, promoter and most of the structural gene for β-galactosidase was linked to the plasmid pBR322, creating a plasmid designated pMG4. Analysis With restriction endonucleases indicates that the lac operon and the ampicillin resistance gene in pMG4 are transcribed in the same direction and the Hind Ⅲ site located in the lambda DNA region of the lac insert is near the other site in the tetracycline resistance gene of pBR322. A plasmid, designated pMG401, containing only one EcoR1 site located in the β-galactosidase gene near the carboxyl terminal was obtained by transforming Escherichia coli C_(600) with Hind Ⅲ fragments of pMG4 and selecting for Ap~rTc~slac~+ transformants. The plasmid pMG401 is a molecular cloning vehicle in which transcription of a heterologous DNA fragment inserted at the EcoR1 site can be regulated by the lac operator and promoter. The expression of the lac operon in various bacterial strains harbouring pMG4, pMG401 or lambda plac5 was observed by measurement of β-galactosidase specific activity.

用包含乳糖操纵子的λplac 5 EcoR1 DNA片段与质粒pBR 322重组,获得一个重组质粒pMG4。几种限制性内切酶的分析结果表明,pMG4上乳糖操纵子(lac)和抗氨基苄青霉素基因的转录方向一致;Iac插入片段λ区的Hind Ⅲ切点和pBR 322抗四环素基因上的Hind Ⅲ切点相邻近。用pMG4 Hind Ⅲ片段转化大肠杆菌C_(600),筛选Ap~rTc~s lac~+转化体,得到另一个lac重组质粒pMG401,在β-半乳糖苷酶结构基因近羧基端有一个EcoR1切点,插入外源基因可以置于lac控制下而实现表达。同时,我们还测定了包含pMG4、pMG401或λplac 5不同细胞的β-半乳糖苷酶活力,对lac的表达作了分析。

The lambda plac5 EcoR1 DNA fragment containing the lac operator,promoterand most of the structural gene for β-galactosidase was linked to the plasmidpBR322,creating a plasmid designated pMG4.Analysis With restriction endonucle-ases indicates that the lao operon and the ampicillin resistance geno in pMG4 aretranscribed in the same direction and the Hind Ⅲ site located in the lambda DNAregion of the lac insert is near the other site in the tetracycline resistance gene ofpBR322.A plasmid,designated pMG401,containing...

The lambda plac5 EcoR1 DNA fragment containing the lac operator,promoterand most of the structural gene for β-galactosidase was linked to the plasmidpBR322,creating a plasmid designated pMG4.Analysis With restriction endonucle-ases indicates that the lao operon and the ampicillin resistance geno in pMG4 aretranscribed in the same direction and the Hind Ⅲ site located in the lambda DNAregion of the lac insert is near the other site in the tetracycline resistance gene ofpBR322.A plasmid,designated pMG401,containing only one EcoR1 site located inthe β-galactosidase gene near the carboxyl terminal was obtained by transformingEscherichia coli C_(600) with Hind Ⅲ fragments of pMG4 and selecting for Ap~rTc~slac~+transformants.The plasmid pMG401 is a molecular cloning vehicle in which tra-nscription of a heterologous DNA fragment inserted at the EcoR1 site can beregulated by the lac operator and promoter.The expression of the lao operon invarious bacterial strains harbouring pMG4,pMG401 or lambda plac5 was observed bymeasurement of β-galactosidase specific activity.

用包含乳糖操纵子的λplac 5 EcoR1 DNA 片段与质粒pBR 322重组,获得一个重组质粒pMG4。几种限制性内切酶的分析结果表明,pMG4上乳糖操纵子(lac)和抗氨基苄青霉素基因的转录方向一致;Iac 插入片段λ区的Hind Ⅲ切点和pBR 322抗四环素基因上的Hind Ⅲ切点相邻近。用pMG4 Hind Ⅲ片段转化大肠杆菌C_(600),筛选Ap~r Tc~s lac~+转化体,得到另一个lac 重组质粒pMG401,在β-半乳糖苷酶结构基因近羧基端有一个EcoR1切点,插入外源基因可以置于lac 控制下而实现表达。同时,我们还测定了包含pMG4、pMG401或λplac 5不同细胞的β-半乳糖苷酶活力,对lac 的表达作了分析。

Staphylococcus aureus strain 307,isolated from a patient,was a multiple resistance to antibiotic,including chloramphenicol-resistance (Cm R),kanamycin-resistance (KmR) and tetracycline-resistance (Tc R ).The result revealed that chloramphenicol-resistance,kanamycin-resistance and tetracycline-resistance were determined by three plasmids,respectively,i.e.,pC307,pK307 and pT307.These plasmids have been transferred into Bacillus subtilis strain Ki-2 and 168...

Staphylococcus aureus strain 307,isolated from a patient,was a multiple resistance to antibiotic,including chloramphenicol-resistance (Cm R),kanamycin-resistance (KmR) and tetracycline-resistance (Tc R ).The result revealed that chloramphenicol-resistance,kanamycin-resistance and tetracycline-resistance were determined by three plasmids,respectively,i.e.,pC307,pK307 and pT307.These plasmids have been transferred into Bacillus subtilis strain Ki-2 and 168 via transformation.Transformants with the character of CmR KmR TcR were obtained.In the present paper we have observed the expression of these plasmids in B.subtilis strain Ki-2 and 168,their compatibility and stability in the new hosts.

Staphylococcus aureus 307株是临床分离的一多重耐药株,其中包括抗氯霉素(Cm~R),抗卡那霉素(Km~R)和抗四环素(Tc~R)。实验结果表明,这三种抗药性是分别为三个质粒所决定的。我们把它们分别称为pC307、pK307和pT307。通过转化把三个质粒引入Bacillus subtilis Ki-2和168株,获得Cm~R Km~R Tc~R转化体。本文研究了这些质粒所携带的抗药基因在Ki-2和168株中的表达,以及质粒在新宿主中的相容性和稳定性。

 
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