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glycoprotein
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  糖蛋白
     The Role of PACS-1 in trans-Golgi Network Localization of Herpes Simplex Virus 1 Glycoprotein B
     PACS-1在单纯疱疹病毒I型糖蛋白B定位于分泌高尔基体网络中的作用
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     Fused expression of hantavirus glycoprotein G1 and partial NP in insect cells and study on its immunological properties
     汉坦病毒囊膜糖蛋白G1与核蛋白部分片段在昆虫细胞中的融合表达及免疫学特性研究
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     Sequencing and analysis of complete genome of 84FLi strain-a Hantaan virus,isolated in Xi'an and Construction of Gl and G2 glycoprotein baculovirus expressi
     汉滩病毒西安分离毒株84FLi的全基因序列测定与分析及G1和G2糖蛋白杆状病毒表达载体的构建
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     Sequence Analyses of Envelope Glycoprotein Genes of Chinese Isolates of Canine Distemper Virus and Immune Effect Induced by DNA Vaccine
     犬瘟热病毒中国分离株囊膜糖蛋白基因序列比较及其DNA疫苗的免疫效力
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     The Construction of DNA Vaccine of Herpes Simplex Virus Type Ⅱ Glycoprotein D with Chemokine MIP-1α and Preliminary Immunity in Mice
     单纯疱疹病毒Ⅱ型gD糖蛋白加趋化因子MIP-1α核酸疫苗的构建及初步免疫观察
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  “glycoprotein”译为未确定词的双语例句
     Identification and Comparison of Neutralizing Epitopes of Structural Glycoprotein E2 & E~(rns) of Classical Swine Fever Virus Using Phage Display
     应用噬菌体展示技术进行猪瘟病毒结构(糖)蛋白E2和E~(rns)中和抗原表位鉴定和比较
短句来源
     The Expression and Function of Dystrophin Glycoprotein Complex in Neuronal Cholinergic Synapses
     Dystrophin Glycoprotein Complex在神经元性胆碱能突触中的表达和功能的研究
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     Ⅰ. Interaction between PPARγ and Ligands or Relative Nuclear Receptor Ⅱ. Thermodynamic and Kinetic Investigation of SARS_CoV Spike Glycoprotein Immunological Fragment
     Ⅰ.PPARγ与小分子以及相关受体蛋白的相互作用研究 Ⅱ. SARS冠状病毒刺突蛋白免疫片段的热力学和动力学研究
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     Study on Suicidal DNA Vaccine Encoding Glycoprotein C (gC) of Pseudorabies Virus and Immunoenhancement Effect of VP22 Protein Transduction on the Vaccine
     伪狂犬病毒gC基因“自杀性”DNA疫苗及VP22蛋白转导的免疫增强效应研究
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     Study on the Immune Efficacy of Recombinat Fowlpox Virus Expressing the Major Antigen Epitope Genes of Glycoprotein B and D of Infectious Laryngotracheitis Virus and the Constant Region Genes of Heavy Chain of Chicken IgY
     表达ILTV gB及gD主要抗原表位基因和鸡IgY重链恒定区基因重组鸡痘病毒免疫效力的研究
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  相似匹配句对
     P-GLYCOPROTEIN AND VOLUME REGULATION
     P-gp和细胞容积调节
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     P-glycoprotein and intractable epilepsy
     P-糖蛋白与难治性癫痫的关系
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  glycoprotein
Molecular Modeling of P-Glycoprotein and Related Drugs
      
This paper summarizes the results from molecular modeling of various multidrug resistance (MDR) modulators and the MDR protein P-glycoprotein (P-gp).
      
The change of serum leptin and its relationship with platelet membrane glycoprotein Ib in patients with coronary heart disease
      
The aim of this paper was to investigate the change of serum leptin and its relationship with platelet membrane glycoprotein Ib (GP Ib) in patients with coronary heart disease (CHD).
      
Low-Molecular-Weight Glycoprotein from Cattle Blood Serum: Structure and Properties
      
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An α_1-glycoprotein, TCA and heat labile, with trypsin inhibitor activity, was

(一)本文用硫酸銨分段沉淀和DEAE纤維素柱层析方法从牛血浆中提取了一种对三氯乙酸不稳定的α_1-糖蛋白,經鉴定系超离心、电泳和免疫純。光散射法測定分子量为66,500,超离心-扩散法測定为67,000(偏微比容取0.65)。 (二)該蛋白质具有抑制胰蛋白酶的活力,酶与糖蛋白的克分子比值是2:1。 (三)該蛋白质与胰蛋白酶結合后,仍与其相应抗血清产生沉淀反应,文中对其抑制活力中心和抗原决定簇的关系作了討論。

The method of separation and identification of the glycoprotein in Hevea brasiliensis latex is described in this article. The fractional precipitation of F serum (frozen serum) was realized with ammonium sulphate and the precipitate was desalted by dialysis or with Sephadex G-25. When the protein solution obtained was subjected to the electrophoresis on the polyacrylamide gel, three protein bands were recognized. If the above-mentioned protein solution was ultrafiltrated with an ultrafiltering membrane...

The method of separation and identification of the glycoprotein in Hevea brasiliensis latex is described in this article. The fractional precipitation of F serum (frozen serum) was realized with ammonium sulphate and the precipitate was desalted by dialysis or with Sephadex G-25. When the protein solution obtained was subjected to the electrophoresis on the polyacrylamide gel, three protein bands were recognized. If the above-mentioned protein solution was ultrafiltrated with an ultrafiltering membrane having a molecular weight cut-off value of about 11,700 daltons (the molecular weight of cytochrome C), the filtrate showed only one protein band after being examined with the electrophoresis on the polyacrylamide gel. It was identified as a kind of glycoprotein by the specific color-producing reaction. If the F serum was directly separated on Sephadex G-25 column and the protein fraction obtained was ultrafiltrated with the same method as mentioned above, it could be found that this filtrate was a kind of glycoprotein.

本文介绍从天然胶乳中提取和鉴定糖蛋白的方法。将F乳清(冷冻乳清)用硫酸铵分部沉淀,沉淀物经过透析或用Sephadex G—25重复脱盐,所得的蛋白质溶液用聚丙烯酰胺凝胶电泳鉴定,得到三条蛋白质谱带。将上述蛋白质溶液,用分子量截止值为11,700(细胞色素c的分子量)的超滤膜进行超滤,滤出液用聚丙烯酰胺凝胶电泳鉴定,只得一条蛋白质谱带,用糖蛋白的特异显色方法显色,证明它是一种糖蛋白。将F乳清用Sephadex G—25凝胶柱直接分离,所得到的蛋白质级分用同样的方法进行超滤,滤出液经过鉴定是一种糖蛋白。

Fresh pollen of Cucurlita pepo and Luff a cylindrica was extracted for 1-50 min.in a cold isotonic Tris-HCl buffer containing 10% sucrose,100 ppm Ca++and 100 ppm boron.This treatment preserved pollen viability and kept the pollen grains from bursting,thus preventing the leakage of the nonpollen-wall proteins.After partial purification by 90% (NH4)2SO4 precipitation (or by gel filtration),the pollen-wall diffusates were subjected to spectrophotometric analysis at 280 nm and 260 nm.Optical readings showed that...

Fresh pollen of Cucurlita pepo and Luff a cylindrica was extracted for 1-50 min.in a cold isotonic Tris-HCl buffer containing 10% sucrose,100 ppm Ca++and 100 ppm boron.This treatment preserved pollen viability and kept the pollen grains from bursting,thus preventing the leakage of the nonpollen-wall proteins.After partial purification by 90% (NH4)2SO4 precipitation (or by gel filtration),the pollen-wall diffusates were subjected to spectrophotometric analysis at 280 nm and 260 nm.Optical readings showed that in C.pepo,protein released in 1 min.had reached nearly one half of the total protein content.Maximum absorption occurred in 20 min.followed by a levelling off.Polyacrylamide gel electrophoresis showed that the pollen-wall diffusates of both plant species contained 7 main protein zones.Three bands gave positive PAS reaction,indicating they were glycoproteins.Paper chromatographic analysis showed that the carbohydrate fraction of the glycoprotein of both plant species consisted of galactose,arabinose and some other sugar which has not yet been identified.Pollen-wall protein of both plant species showed isozyme bands of esterase,acid phos-photase,but no alkaline phosphotase.After dissociation in SDS and mercapto-ethanol,and electrophoresis in SDS gels,many bands appeared in pollen-wall diffusates of both plant species.By comparing their mobilities with those of standard profeins of known molecular weights,the molecular weights of some of the major fractions of pollen-wall diffusates of Luff a cylindrica were estimated to be 12,000,25,000,43,000,46,000 and 76,000.Intergeneric crossing of Luffa cylindrica Cucurbita,moschata is highly incompatible,but application of pollen-wall diffusate of Luffa cylindrica (containing 2-5mg/ml protein) to the stigma prior to pollination with pollen of Cucurbita moschata resulted in 5% fruit setting.The resultant fruits contained viable seeds.

将葫芦科植物丝瓜和西葫芦当天开放的新鲜花粉放入0.025M,pH7.8,Tris-HCI缓冲液中,在1—50分钟不等时间内提取(含10%蔗糖,100ppm Ca~(++),100ppm硼)滤液用90%饱和度的硫酸铵沉淀,透析除盐(或过Sephadex G-25凝胶柱),然后在紫外光光度计280nm下测OD值。 不同时间取样测定表明,西葫芦花粉在1分钟内提取的滤液中蛋白质含量已达全部释放的蛋白质的一半左右,以后缓慢增加,20分钟后不再继续释放。 西葫芦花粉渗出液经聚丙烯酰胺凝胶电泳分离,考马斯亮蓝染色,可分出明显的7个区带,19条蛋白带。丝瓜花粉经同样处理后也可分出7个区带,18条蛋白带。两者电泳图型不完全一样。 两种植物花粉壁蛋白都显示出中性酯酶、酸性磷酸酯酶同功酶带,但两者都没有碱性磷酸酯酶。 两种植物的花粉壁蛋白的电泳胶条,用过碘酸-Schiff试剂染色后出现三条红色带,表明这部分蛋白质是糖蛋白。蛋白样品经酸水解后纸层析分离,表明两种植物花粉壁蛋白均含有三种糖:半乳糖,阿拉伯糖及一个尚未判明的糖。 两种植物的花粉壁蛋白都有热稳定的成份。 花粉壁蛋白经SDS、β-巯基乙醇凝胶电泳后出现多条染色带,经与在同样...

将葫芦科植物丝瓜和西葫芦当天开放的新鲜花粉放入0.025M,pH7.8,Tris-HCI缓冲液中,在1—50分钟不等时间内提取(含10%蔗糖,100ppm Ca~(++),100ppm硼)滤液用90%饱和度的硫酸铵沉淀,透析除盐(或过Sephadex G-25凝胶柱),然后在紫外光光度计280nm下测OD值。 不同时间取样测定表明,西葫芦花粉在1分钟内提取的滤液中蛋白质含量已达全部释放的蛋白质的一半左右,以后缓慢增加,20分钟后不再继续释放。 西葫芦花粉渗出液经聚丙烯酰胺凝胶电泳分离,考马斯亮蓝染色,可分出明显的7个区带,19条蛋白带。丝瓜花粉经同样处理后也可分出7个区带,18条蛋白带。两者电泳图型不完全一样。 两种植物花粉壁蛋白都显示出中性酯酶、酸性磷酸酯酶同功酶带,但两者都没有碱性磷酸酯酶。 两种植物的花粉壁蛋白的电泳胶条,用过碘酸-Schiff试剂染色后出现三条红色带,表明这部分蛋白质是糖蛋白。蛋白样品经酸水解后纸层析分离,表明两种植物花粉壁蛋白均含有三种糖:半乳糖,阿拉伯糖及一个尚未判明的糖。 两种植物的花粉壁蛋白都有热稳定的成份。 花粉壁蛋白经SDS、β-巯基乙醇凝胶电泳后出现多条染色带,经与在同样条件下电泳的标准蛋白比较,其中主要蛋白的分子量范围为11700—12000、25000、43000、46000和76000道尔顿。 在丝瓜(母本)和南

 
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