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lectin
相关语句
  凝集素
     The Relationship between Mannose-binding Lectin (MBL) and Rheumatic Heart Disease and Recombinant MBL Protein Expression
     甘露糖结合凝集素与风湿性心脏病的关系及其重组蛋白的表达
短句来源
     Cloning and Expression of Receptor-like Protein Genes Mediating Verticillium Dahliae Resistance and a Mannose-binding Lectin Gene from Plants
     植物抗大丽轮枝菌受体类蛋白基因及甘露糖结合型凝集素基因的克隆与表达
短句来源
     Cloning, Characterization and Functional Study of a New C-type Lectin, CD23L
     一种新的C型凝集素,CD23L的克隆、特性和功能研究
短句来源
     Studies of a New Lectin FRIL on the Capability to Maintain Hematopoietic Stem/progenitor Cells in Vitro and Its Mechanism
     凝集素FRIL对造血干/祖细胞多潜能性的维持及其机理研究
短句来源
     Regulatory Roles of Mannan-binding Lectin in Differentiation and Functions of Antigen Presenting Cells
     甘露聚糖结合凝集素对抗原提呈细胞分化与功能的调节作用
短句来源
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  外源凝集素
     Agglutination of lectin ( ConA and PHA ) with "HO" and "O" antigens of Salmonella groups and inhibition of saccharide were assayed.
     用外源凝集素ConA、PHA对沙门氏菌“HO”、“O”抗原作了凝集及糖类抑制试验。
短句来源
     Agglutination capacity of lectin (ConA and PHA) with "HO" and "O" Antigens of salmonella groups and inhibjtion Test by Saccharide.
     外源凝集素ConA与PHA对沙门氏菌“HO”、“O”抗原凝集及糖抑制试验
短句来源
     The cDNAs of snowdrop lectin mature protein and its precursor protein, GNA12 and GNA34, were inserted downstream of 35S promoter with double enhancer and “Ω” fragment of TMV RNA cDNA in the binary vector pBin438,or the phloem specific CoYMV promoter in the vector pBcop1,respectively,resulting in the construction of four plant expression vectors pBGna12,pBGna34,or pBCGna12 and pBCGna34.Leave stripes of Nicotiana tabacum var.K326 were transformed with A.
     将编码雪花莲外源凝集素成熟蛋白的cDNAGNA12和其前体蛋白cDNAGNA34插入到二元载体pBin438的双倍增强子CaMV35S启动子或二元载体pBcop1的CoYMV启动子下游,分别构建成植物表达载体pBGna12、pBGna34、pBCGna12和pBCGna34。
短句来源
     Three kinds of lectin (ConA, WGA, DBA) receptors were examined and analysed in 84 cases of benign and malignant nodules of the thyroid gland by means of the ABC immunohistochemical method.
     采用ABC组化技术,对84例甲状腺良、恶性结节上皮细胞或上皮源性细胞做了三种外源凝集素染色,即ConA,WGA,DBA。
短句来源
     In this research, we report here molecular genetic analyses and insect resistance of transgenic plants, revealing the integration and inheritance of transgenes in the progeny (R2 and R3) of these plants, which delivered by particle bombardment, by cotransformation the SBT1 (soy bean trypsin inhibitor) gene and GNA (snowdrop lectin) gene and hpt (hygromycin phosphotransferase) gene into Indica rice (Oryza saliva L. cvs. Zhuxian B) with three plasmids, carrying a total of three genes.
     在本研究中,利用分子检测和抗虫实验研究,证明了利用基因枪导入水稻的外源基因雪花莲外源凝集素(GNA)基因、大豆蛋白酶抑制剂(SBTi)基因和潮霉素抗性(hpt)基因在转基因抗虫水稻后代(R2代和R3代)基因组中的整合和遗传稳定性及抗虫效应。
短句来源
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  植物凝集素
     Concanavalin A (Con A), is a kind of lectin separated from sword bean.
     刀豆蛋白A(Concanavalin A,Con A),是从刀豆中提取的植物凝集素,能广泛的刺激淋巴细胞和单核细胞。
短句来源
     A newly cloned lectin, FRIL (Flt3 receptor-interacting lectin), was identified to be capable of preserving primitive progenitors in vitro without loss of repopulating capacity.
     最近发现的一种植物凝集素FRIL(Flt3 receptor-interacting lectin),能够于离体条件下长期维持造血干细胞的自我更新能力。
短句来源
     Bioinformatical analyses suggest that IPPDN45M contain a complete gene that encodes a lectin or a lectin-like protein named IPPDN45M-P. Amino acid components and orders in the putative IPPDN45M-P indicate that the protein contains a functional domain GAL-Lectin found in other lectins.
     生物信息学分析(基因预测)表明IPPDN45M中包含了一个完整的基因IPPDN45M-G,其编码的蛋白质IPPDN45M-P可能是一种植物凝集素蛋白或类似物,并包含一个GAL_Lectin活性结构域。
短句来源
     By SDS-PAGE and gel filtration, the molecular weight of HMWP was estimated to be about 114.8 kD and 115.0 kD respectively. Identified by western blotting of lectin, HMWP was a kind of glycoprotein with less kinds of sugar, containing mannose (Man) and N-acetylglucosamine (GlcNAc).
     多种植物凝集素的Westernblotting鉴定表明 ,HMWP是一个糖基种类较少的糖蛋白 ,含有Man和GlcNAc。 SDS PAGE和凝胶过滤分别测得HMWP的分子量为 114 .8kD和 115 .0kD。
短句来源
     Preliminary Identification of Human Oviduct Glycopro- teins Binding to Lectin BS - 1
     人输卵管中与植物凝集素BS-1结合糖蛋白初步鉴定
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  “lectin”译为未确定词的双语例句
     The nested PCR and semi-nested PCR tests were used to detect the transgenic ingredient in Roundup Ready soybean oil,the results showed that Lectin gene(112bp),CaMV35S gene(147 bp) and CP4-EPSPS gene(205 bp)could be amplified from crude soybean oil but not from finished product of soybean oil.
     利用nested PCR和semi-nested PCR检测大豆(Roundup Ready)油中的转基因成分发现,该方法能够检测到大豆原油中的Lectin基因(112bp)、CaMV35S基因(147bP)和CP4-EPSPS基因(205bp),检测灵敏度达到10-6ng/μl;
短句来源
     Effects of mannan-binding lectin on TNF-α and IL-12 production induced by lipopolysaccharide in differentiated and activated THP1/CD14 cells
     MBL对LPS刺激的不同分化和活化状态THP1/CD14细胞产生TNF-α和IL-12的影响
短句来源
     Binding of galactose to the lectin was assayed spectro- photometrically and the association constant for galactose was 6×10~3 L/ Mol.
     用分光光度分析法测定半乳糖与PA-Lectin的结合,其结合常数为6×10~3L/Mol。
短句来源
     6. 35S, lectin, Pat and Cryllla were cloned to be positive control.
     6.克隆了35S,leclin,Pat和CryⅢa四个质粒作为阳性对照。
短句来源
     There were significant correlations between the expressions of CD44v6 and P lectin, and the lung metastasis of osteosarcoma.
     CD44v6和P选择素表达与骨肉瘤肺转移有明显相关性。
短句来源
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  lectin
Isolation and Partial Characterization of Lectin from the Venom of Vietnamese Scorpion Buthus occitanussp.
      
A lectin was isolated from the venom of scorpion Buthus occitanussp.
      
The homogeneous lectin preparation consisted of homodimeric molecules with a subunit Mrof 9.3 kDa.
      
Glycine, alanine, and serine dominated in the lectin amino acid composition.
      
The lectin was a glycoprotein containing 20% carbohydrates (predominantly mannose and glucose).
      
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A lectin was isolated and purified from snow mountain-bean (Phascolus sp.) Seeds through chromatography on Sephadex G-150. The isolated lectin showed a single band in both ployacrylamide gel electrophoresis at pH 9.0 or pH4. 5. The purified lectin is a glycoproteins.This lectin agglutinates the erythrocytes of rabbit, pig, dog, mouse, rat, mice, pigeon, goose, duck, chicken, turtle, toad, carp, crucian carp, eel, loach and human the erythrocytes of with no specificity for human blood...

A lectin was isolated and purified from snow mountain-bean (Phascolus sp.) Seeds through chromatography on Sephadex G-150. The isolated lectin showed a single band in both ployacrylamide gel electrophoresis at pH 9.0 or pH4. 5. The purified lectin is a glycoproteins.This lectin agglutinates the erythrocytes of rabbit, pig, dog, mouse, rat, mice, pigeon, goose, duck, chicken, turtle, toad, carp, crucian carp, eel, loach and human the erythrocytes of with no specificity for human blood groups, but does not agglutinate buffalo, cattle and goat. The agglutinating activity is inhibited by D-mannose, D-glucose, N-acetylglucosamine and oligomer of N-acetylglucosamine.The lectin has mitogenic activity on animal or human peripheral blood lymphocytes.

本文报导从雪山豆种子中分离纯化凝集素与其部分性质.种子磨粉、浸取,经硫酸铵分级,再以Sephadex G—150疑胶过滤纯化而得.所得样品经聚丙烯酰胺盘状电泳鉴定,为单一蛋白带.以糖蛋白染色法鉴定,该凝集素为糖蛋白。它具有凝集动物红细胞和促淋巴细胞有丝分裂的活性.

A plant protein, designated pinellin, was isolated from the juice of the Chinese traditional medicine pinellia (Pinellia ternata Breit.) and crystallized in a dilute phosphate buffer, pH 6.8, at 28~30℃. It exhibits the activity of terminating early pregnancy. Hypodermic injeotion of 250μg of pinellin per mouse on the 7th day of gestation induced a termination of pregnancy in 50% of the mice tested. Pinellin is also a plant lectin, as it exhibits a specific hemagglutinating activity to rabbit erythrocytes...

A plant protein, designated pinellin, was isolated from the juice of the Chinese traditional medicine pinellia (Pinellia ternata Breit.) and crystallized in a dilute phosphate buffer, pH 6.8, at 28~30℃. It exhibits the activity of terminating early pregnancy. Hypodermic injeotion of 250μg of pinellin per mouse on the 7th day of gestation induced a termination of pregnancy in 50% of the mice tested. Pinellin is also a plant lectin, as it exhibits a specific hemagglutinating activity to rabbit erythrocytes at a concentration as low as 2μg/ml. The molecular weight of pinellin is 44,000 as determined by gel fitration. Pinellin dissociated into subunits in the presence of sodium dodecyl sulfate or guanidine hydrochloride. The amino acid composition and N-terminal amino acid residues of pinellin have been analysed. It is devoid of hexose. There are suggestions that pinellin may represent a family of isoproteins with very similar molecular size and structure.

从中草药半夏(Pinellia ternata Breit.)的鲜汁中分离出一种植物蛋白质组分,定名为半夏蛋白。它在稀磷酸缓冲液,pH 6.8,28~30℃结晶。它具有抑制早期妊娠的活力。给怀孕七天的小白鼠每只皮下注射250微克半夏蛋白,50%注射过的小白鼠引起流产。半夏蛋白也是一种植物凝集素,它与兔红血球有专一的血凝活力,浓度低至每毫升2微克仍有凝集作用。用凝胶过滤法测定,半夏蛋白分子量为44,000。十二烷基硫酸钠或胍盐存在下,半夏蛋白解聚成亚基。测定了半夏蛋白的氨基酸组成和N-末端氨基酸残基。半夏蛋白不含己糖。有迹象表明半夏蛋白可能是其分子大小、结构与性质极为相近的一组蛋白。

Three immobilized acetylcholinesterases of pig brain caudata nucleus have been prepared by different methods. One of them, the sepharose 4B-Ab-AchE, is most stable to heat. The sepharose 4B-Pea. Lectin-AchE is least stable to heat, the enzyme activity being significantly increased after treatment with antibody. This antibody effects a partial resteration of the enzymatic activity following its interaction with the heat inactivated sepharose 4B-Pea. Lectin-AchE.The apparent Michaelis constant and...

Three immobilized acetylcholinesterases of pig brain caudata nucleus have been prepared by different methods. One of them, the sepharose 4B-Ab-AchE, is most stable to heat. The sepharose 4B-Pea. Lectin-AchE is least stable to heat, the enzyme activity being significantly increased after treatment with antibody. This antibody effects a partial resteration of the enzymatic activity following its interaction with the heat inactivated sepharose 4B-Pea. Lectin-AchE.The apparent Michaelis constant and reaction velocity of the enzyme did not change after treatment with antibody. It was shown that the antibody might combine with a non-active site of acetylcholinesterase without affecting the conformation of its active site.

采用不同的固定化方法制备了三种固定化猪脑尾状核乙酰胆碱酯酶,其中琼脂糖-抗体-乙酰胆碱酯酶热稳定性最好。抗体与热稳定性最差的琼脂糖-青豌豆植物凝集素-乙酰胆碱酯酶反应后,酶的热稳定性明显提高。抗体还能部分恢复热失活的琼脂糖-青豌豆植物凝集素-乙酰胆碱酯酶的活力。酶与抗体反应前后的表观米氏常数K_m及反应速度没有明显变化,表明抗体是结合在酶的非活性部位,没有或很少影响酶的活性中心的构象。

 
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