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bacteriophage
相关语句
  噬菌体
     In Bacteriophage splitting and ESP,there were 82.8%(96/116) tally with masculine-rate,74.1%(80/108)tally with negative-rate and 78.6%(176/224) tally with both.
     噬菌体法与ESP法阳性符合率为82.8%(96/116),阴性符合率为74.1%(80/108),总体符合率为78.6%(176/224)。
短句来源
     [Results]For bacteriophage MS2 exposured to 370 μW/cm2 for 10 min,or 680 μW/cm2,5 min,or 1 130 μW/cm2,3 min,the disinfection level(LIV≥4.00 log10)had been achieved and the LIV were 4.49 log10,4.52 log10,and 4.16 log10 respectively.
     [结果]MS2噬菌体在UVC照射下:370 μW/cm2,10min,或680 μW/cm2,5min,或1130 μW/cm2,3min,达到消毒水平(LIV≥4.00log10),LIV分别为4.49 log10、4.52 log10和4.16 log10;
短句来源
     Identification of 53×10~3 protein gene of bacteriophage PaP3
     PaP3噬菌体53×10~3蛋白编码基因的确定
短句来源
     2In Bacteriophage splitting and smearstest,there were 93.5%(58/62) tally with masculine-rate,59.3%(96/162) tally with negative-rate and 68.8%(154/224)tally with both.
     ②噬菌体法与涂片法阳性符合率为93.5%(58/62),阴性符合率为59.3%(96/162),总体符合率为68.8%(154/224);
短句来源
     Preparation of Packaging Extracts of Bacteriophage λfrom BHB2688 and BHB2690 and in vitro Packaging of λDNA
     从大肠杆菌BHB2688和BHB2690制备噬菌体λ包装蛋白及体外包装λDNA
短句来源
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  细菌噬菌体
     Expression of Cecropin A Genes by Bacteriophage T7 RNA Polymerase
     用细菌噬菌体T7RNA聚合酶高效表达天蚕蛾菌素(CecropinA)基因
短句来源
  细菌噬菌体
     Expression of Cecropin A Genes by Bacteriophage T7 RNA Polymerase
     用细菌噬菌体T7RNA聚合酶高效表达天蚕蛾菌素(CecropinA)基因
短句来源
  “bacteriophage”译为未确定词的双语例句
     Studies on the bacteriophage of glutamic acid-producing bacteria,A.S.1.542(Corynebacterium sp.)
     谷氨酸产生菌A.S.1.542(Corynebacterium sp.)噬菌体的研究
短句来源
     Identification and Analysis of Clones Carrying Bacteriophage T4 Gene 42 and 43 DNA Fragments
     大肠杆菌噬菌体T4基因42和43DNA片段无性繁殖系的鉴定和分析
短句来源
     Nucleotide Frequency Analysis of Bacteriophage φX174 Genome
     病毒φX174基因组的核苷酸频率分析
短句来源
     The specific cytotoxic response was induced targeting A431 cells in experimental groups of T7415-1b-90 and T7415-1b-132 bacteriophage,and the OD_(E+T) had statistically significant difference compared with control groups (P<0.01).
     T7415-1b-90和T7415-1b-132实验组均诱导产生对A431细胞的特异性杀伤作用,检测的ODE+T与对照组相比具有显著性差异(P<0.01);
短句来源
     Isolation and Identification of Bacteriophage Verotoxin 2 Gene from E. coli O157:H7
     大肠杆菌O157:H7中编码vt2基因的噬菌体的分离与鉴定
短句来源
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  bacteriophage
Pigments isolated from macromycetes and applied at a much lower concentration than those obtained from micromycetes prevented damage to bacteriophage-λ DNA induced by products of peroxidase-mediated degradation of aminobiphenyls.
      
Transformation of a Fragment of β-Structural Bacteriophage T4 Adhesin to Stable α-Helical Trimer
      
Gene product 12 of bacteriophage T4, adhesin, serves to adhere the virus to host cells.
      
Expression and Properties of Bacteriophage T4 Gene Product 11
      
A plasmid vector for expression of bacteriophage T4 gene product 11 (gp11) in E.
      
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The morphological and serological characteristics of the bacteriophage werestudied.Plaques of the phage were clear,about 0.3—0.7mm in diameter afterincubation for 24hrs at 32℃.Electron microscopic observation by means ofnegative staining with PTA recognized the phages as tadpole-shaped,theirparticles having polyhedral head.The sizes of head and tail were 545.9×642.9(?)and 2219.8×105.5(?),respectively.K va lues of the phage P-_A and P-_B wereabout 359 and 370 respectively.Accordingly,it is clear that the...

The morphological and serological characteristics of the bacteriophage werestudied.Plaques of the phage were clear,about 0.3—0.7mm in diameter afterincubation for 24hrs at 32℃.Electron microscopic observation by means ofnegative staining with PTA recognized the phages as tadpole-shaped,theirparticles having polyhedral head.The sizes of head and tail were 545.9×642.9(?)and 2219.8×105.5(?),respectively.K va lues of the phage P-_A and P-_B wereabout 359 and 370 respectively.Accordingly,it is clear that the phage P-_A andP-_B are the same species.The growth characteristics of the phage were examined by the one-step growthex periment.The latent period of the phage was 61 minutes,the rise perod30 minutes and the average burst size was about 66.The adsorption of the hostbacteria is very low,about 12.9%,the multiplicity of infection is about 0.088.

本文对谷氨酸产生菌A.S.1.542噬菌体的形态学和血清学特性以及一步生长曲线进行了研究。这种噬菌体的噬菌斑园形透明,培养24小时噬菌斑大小为0.3—0.7mm。噬菌体呈蝌蚪状,多角形头部,尾较长,无尾鞘,尾不收缩。头部大小为545.9×642.9,尾部为2219.8×105.5(?)。将大小不同的噬菌斑单株分离纯化,得到P—_A 和P—_B 两株,进行血清中和反应实验的结果表明,这两株噬菌体不但能够起交义中和反应,而且中和速度常数K 值非常接近,分别为K_A=359;K_B=370。因此,P—_A 和P—_B 是属于同一血清型。通过一步生长实验,定量地测定了该噬菌体的潜伏期、上升期和裂解量。噬菌体的吸附此例为12.9%;单独感染的感染复数为0.088;潜伏期为61分钟;上升期为30分钟;每个被感染细胞的平均裂解量为66.3个噬菌体。

A wilting type of bacterial blight has been found to occur widely in thehybrid ricer, Nan-you No. 2 and Nan-you No. 3. The disease development wasconspicuous especially in the later seedling and vigorous tillering stages.The characterisntic symptoms produced were,(1)the youngest leaf or 1-2leaves fold up and roll along the midrib;(2)the cutting surface of thestem base exudates a yellow viscid substance after being pressed;(3)therest leaves become wilting and the diseased plant eventually dies. The symptomsof...

A wilting type of bacterial blight has been found to occur widely in thehybrid ricer, Nan-you No. 2 and Nan-you No. 3. The disease development wasconspicuous especially in the later seedling and vigorous tillering stages.The characterisntic symptoms produced were,(1)the youngest leaf or 1-2leaves fold up and roll along the midrib;(2)the cutting surface of thestem base exudates a yellow viscid substance after being pressed;(3)therest leaves become wilting and the diseased plant eventually dies. The symptomsof the late infected plant were similar to the commen bacterial leaf blight. The causal organism of the wilting type was compared with one of thecommon type isolate in cultural characteristics, physilogical and biochemicalproperties, serological reactions and bacteriophage reactions, there was nodifference to be found between them. However they were distinctly differentin pathogenicity and in the symptoms produced on the rice plant. Isolatesfrom specimens showing wilting symptoms varied also in degree of infectivity. The relation between the different infections routines of the causalorganism and the inoculation tests revealed that wounds on the root and thestem base were the major sites of infection which lead to the wilting of the plant. It was found that only the isolates of high virulence induced the wiltingsymptoms. It is therefore considered, the causal organism of the wiltingtype of hybrid rice plant is identical to Xanthomonas oryzae (Uyeda etIshiyama) Dowson. It is suggested that in the breeding of the resistant hybrid rices, attent-ion must be taken to employ resistent parents, especially belonging to theR lines.

杂交水稻南优2号、南优3号近年来在湖南普遍发生枯心凋萎现象。病株除在秧苗后期至分蘖盛期呈现枯心、凋萎死亡和茎基有大量菌脓的突出症状外,拔节期后继续发病的稻株逐渐转为叶片受害,甚至在壮苞期引起死穗现象。此外,在调查中还发现少数常规品种偶有发生,但受害轻微。从枯心凋萎病株上分离的菌株与稻白叶枯菌在培养性状、生理生化性状、血清反应以及噬菌体反应等方面的比较测定中,结果表现一致,可以确定这种枯心凋萎现象是由病原细菌Xanthomonas oryzae(Uyeda et Ishiyama)Dowson引致的凋萎型白叶枯病。“凋萎型”和“普通型”的不同菌株存在侵袭力强弱不同的差异。但侵袭力强的菌株并不局限来自凋萎型病株。湖南分离的4个“凋萎型”菌株和1个“普通型”菌株均属侵袭力强的菌株,而广东提供的1个“凋萎型”菌株和湖南的1个“普通型”菌株则属侵袭力弱的菌株。根据人工接种试验,根部及茎基的伤口是病菌导致稻株枯心凋萎的主要侵入途径,但受菌株侵袭力和品种抗病性强弱的影响。供试的侵袭力强或弱的菌株,通过剪根和针刺茎基接种于感病品种南优2号秧苗上,均可导致系统性感染而出现大量枯心凋萎株;但在抗病品种IR26上,侵袭力强的菌株除...

杂交水稻南优2号、南优3号近年来在湖南普遍发生枯心凋萎现象。病株除在秧苗后期至分蘖盛期呈现枯心、凋萎死亡和茎基有大量菌脓的突出症状外,拔节期后继续发病的稻株逐渐转为叶片受害,甚至在壮苞期引起死穗现象。此外,在调查中还发现少数常规品种偶有发生,但受害轻微。从枯心凋萎病株上分离的菌株与稻白叶枯菌在培养性状、生理生化性状、血清反应以及噬菌体反应等方面的比较测定中,结果表现一致,可以确定这种枯心凋萎现象是由病原细菌Xanthomonas oryzae(Uyeda et Ishiyama)Dowson引致的凋萎型白叶枯病。“凋萎型”和“普通型”的不同菌株存在侵袭力强弱不同的差异。但侵袭力强的菌株并不局限来自凋萎型病株。湖南分离的4个“凋萎型”菌株和1个“普通型”菌株均属侵袭力强的菌株,而广东提供的1个“凋萎型”菌株和湖南的1个“普通型”菌株则属侵袭力弱的菌株。根据人工接种试验,根部及茎基的伤口是病菌导致稻株枯心凋萎的主要侵入途径,但受菌株侵袭力和品种抗病性强弱的影响。供试的侵袭力强或弱的菌株,通过剪根和针刺茎基接种于感病品种南优2号秧苗上,均可导致系统性感染而出现大量枯心凋萎株;但在抗病品种IR26上,侵袭力强的菌株除引致病株部分叶片呈现症状外,也可引致少量枯心凋萎株,而侵袭力弱的菌株,仅引致部分叶片发病。

By means of methylated albumin kieselguhr (MAK) column, we have isolated andpurified plasmids pBR322, ColE1, pSC101, pCR1 and bacteriophage λ DNA. Theyhave been characterized by analysis with the EcoR1 restriction endonuclease, electro-phoresis of DNAs on agarose gel, and transformation by R-factors. The resultsindicate that plasmids and λ DNA, prepared on MAK column, are pure, and can beused as vectors for construction of recombinant DNA, and as substrates for the restriction endonucleases.

用甲酯化白蛋白硅藻土(MAK)柱层析方法分离纯化的质粒pBR322,ColE_1,pSC101,pCRl 和λDNA,经EcoR_1限制性内切酶作用,琼脂糖凝胶电泳分析,抗药因子转化,结果表明这些材料可以用作DNA 重组体的载体和限制性内切酶的底物。

 
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