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hepatitis b antigens
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     The incidence of liver cell dysplasia(LCD)and its relationship to hepatitis B antigens (HBsAg) were studied in 116 autopsy and biopsy cases of normal, cirrhotic and cancerous livers by histologic and immunohistologic methods.
     用组织学和免疫组化方法探讨116例肝组织中的肝细胞不典型增生(LCD)发生率和乙型肝炎病毒(HBV)抗原的表达情况.
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     The incidence of liver cell dysplasia (LCD) and its relationship to hepatitis B antigens were studied in 116 autopsy and biopsy cases of normal, cirrhotic and cancerous livers with histologic ahd immunohistochemical methods.
     用组织学和免疫组织化学方法探讨116例肝组织中的肝细胞不典型增生(LCD)发生率和乙型肝炎病毒(HBV)抗原的表达情况。 发现肝细胞癌(HCC)伴肝硬化的LCD发生率为21/30(70%);
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  相似匹配句对
     Hepatitis B vaccination.
     乙型肝炎疫苗免疫
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     Hepatitis C and Detection for it
     丙型肝炎及其检测
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     Die Hepatitis G
     庚型肝炎
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     Hepatitis B Virus
     乙型肝炎病毒
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     SLE AND HLA ANTIGENS
     系统性红斑狼疮(SLE)与HLA抗原
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  hepatitis b antigens
The sensors were fabricated with plasma-polymerized ethylenediamine film (PPF) and nanometer-sized gold particles (nanogold) on which the different hepatitis B antigens/antibodies (markers) were subsequently immobilized.
      
Similarly, hepatitis B is rarely transmitted in this manner, because accurate tests for detecting hepatitis B antigens and antibodies are available.
      


We have developed eleven monoclonal antibodies (MCA) generated against purified hepatitis B antigen (HBsAg). Among them, ten of these MCA showed specificity against the group-specifie-a,determinant of HBsAg, and only one demonstrated antibody activity against the 'd' determinant.The titers of 10 anti-a MCA against adr, adw and ayw subtypes varied between 1/100-1/3200 and that of anti-d against adr and adw subtypes ranged from 1/1600-1/3200 when tested by ID:Five of these MCA have been tested for IgG subclasses....

We have developed eleven monoclonal antibodies (MCA) generated against purified hepatitis B antigen (HBsAg). Among them, ten of these MCA showed specificity against the group-specifie-a,determinant of HBsAg, and only one demonstrated antibody activity against the 'd' determinant.The titers of 10 anti-a MCA against adr, adw and ayw subtypes varied between 1/100-1/3200 and that of anti-d against adr and adw subtypes ranged from 1/1600-1/3200 when tested by ID:Five of these MCA have been tested for IgG subclasses. And we found that one was identified to be IgG2a, all the others were IgG1.

用小鼠骨髓瘤细胞(Sp 2/0) 与HBsAg免疫过的BALB/C小鼠脾细胞融合,获得11株产生抗-HBs的杂交瘤细胞系,其中10株分泌抗-a决定簇的MCA (单克隆抗体),用ID法测得对adr、adw和ayw亚型滴度可达1/100~1/3,200。另一株分泌抗-d决定簇的MCA,对adr和adw亚型ID法滴度为1:1,6004~1:3,200,而对ayw则不起反应。对其中的5株细胞分泌的MCA免疫球蛋白进行亚类分析,证明一株为IgG2a,4株为IgG1。

The incidence of liver cell dysplasia(LCD)and its relationship to hepatitis B antigens (HBsAg) were studied in 116 autopsy and biopsy cases of normal, cirrhotic and cancerous livers by histologic and immunohistologic methods.LCD was found in only one of 64 (1.56%) cases with normal livers,in one of 12 (8.33%) cases with hepatocellular carcinoma (HCC) without cirrhosis, in two of 10 (20%) cases with cirrhosis only, and 21 of 30 (70%)cases with cirrhosis and HCC.Hepatitis B surface antigen...

The incidence of liver cell dysplasia(LCD)and its relationship to hepatitis B antigens (HBsAg) were studied in 116 autopsy and biopsy cases of normal, cirrhotic and cancerous livers by histologic and immunohistologic methods.LCD was found in only one of 64 (1.56%) cases with normal livers,in one of 12 (8.33%) cases with hepatocellular carcinoma (HCC) without cirrhosis, in two of 10 (20%) cases with cirrhosis only, and 21 of 30 (70%)cases with cirrhosis and HCC.Hepatitis B surface antigen (HBsAg) was found in 46 of 52 cases of cirrhotic and(or) cancerous liver tissues,24 of which (52.17%) were associated with LCD, while none was found in 6 cases of HBsAg negative liver tissues (P<0.05).HBsAg was found in dysplasia cells in nine cases.Hepatitis B core antigen (HBcAg) was found in 22/52 cases, 14/22 (63.64%) were associated with LCD, while only 10/30 cases(33.33%) of HBcAg negative liver tissues were associated with LCD.LCD in the former was significantly higher than the later (P<0.05).The incidence of LCD in HBsAg and HBcAg positive cirrhosis was significantly higher than HBsAg and HBcAg negative cirrhotic or HBsAg and HBcAg positive noncirrhotic liver tissues.These findings suggest that LCD is closely associated with HBV and cirrhosis.

用组织学和免疫组化方法探讨116例肝组织中的肝细胞不典型增生(LCD)发生率和乙型肝炎病毒(HBV)抗原的表达情况.发现肝细胞癌(HCC)伴肝硬化的LCD发生率为21/30(70%),单纯肝硬化为2/10(20%),HCC不伴肝硬化1/12(8.33%),正常对照1/64(1.56%).试验组52例中有46例HBsAg阳性,其中24例有LCD(52.17%),而6例HBsAg阴性肝组织中无1例有LCD(P<0.05).52例中22例HBcAg阳性,其中14例有LCD(63.64%);30例HBcAg阴性,10例有LCD(33.33%),前者LCD发生率明显高于后者(P<0.05).结果还发现,HBsAg和HBcAg阳性肝硬化的LCD发生率(65.71%,77.78%)明显高于HBsAg和HBcAg阴性的肝硬化及HBsAg、HBcAg阳性的非肝硬化病例(P<0.01,P<0.05).表明LCD的发生与肝硬化和HBV都有关.

The incidence of liver cell dysplasia (LCD) and its relationship to hepatitis B antigens were studied in 116 autopsy and biopsy cases of normal, cirrhotic and cancerous livers with histologic ahd immunohistochemical methods. LCD was found in only one of 64 (1.56%) cases with normal livers, in one of 12 (8.33%) cases with hepatocellular carcinoma (HCC) without cirrhosis, in two of 10 (20%) cases with cirrhosis only, and 21 of 30 (70%) cases with cirrhosis and HCC. HBsAg was observed in 46 of 52 cases of...

The incidence of liver cell dysplasia (LCD) and its relationship to hepatitis B antigens were studied in 116 autopsy and biopsy cases of normal, cirrhotic and cancerous livers with histologic ahd immunohistochemical methods. LCD was found in only one of 64 (1.56%) cases with normal livers, in one of 12 (8.33%) cases with hepatocellular carcinoma (HCC) without cirrhosis, in two of 10 (20%) cases with cirrhosis only, and 21 of 30 (70%) cases with cirrhosis and HCC. HBsAg was observed in 46 of 52 cases of cirrhotic and (or) can-cerous Liver tissues, 24 of which (52. 17%) were associated with LCD, while none was seen in 6 cases of HBsAg negative liver tissues (P<0. 05). HBsAg was also found in LCD cells of nine cases. HBcAg was present in 22/52 cases, 14/22 (63.64%) related to LCD, only 10/30 cases (33.33%) of HBcAg negative liver tissues with LCD. LCD IN the former was significantly higher than the later (P<0. 05). The incidence of LCD in HBsAg and HBcAg positive cirrhosis was higher than HBsAg and HBcAg negative cirrhotic or HBsAg and HBcAg positive noncirrhotic liver tissues. These results suggest that LCD is closely associated with HBv and cirrhosis.

用组织学和免疫组织化学方法探讨116例肝组织中的肝细胞不典型增生(LCD)发生率和乙型肝炎病毒(HBV)抗原的表达情况。发现肝细胞癌(HCC)伴肝硬化的LCD发生率为21/30(70%);单纯肝硬化为2/10(20%);HCC不伴肝硬化1/12(8.33%);正常对照1/64(1.56%)。试验组52例中46例呈HBsAg阳性,其中24例有LCD(52.17%)。而6例HBsAg阴性肝组织中均未发现LCD(P<0.05)。52例中22例HBcAg呈阳性反应,其中14例有LCD(63.64%);30例HBcAg阴性,10例有LCD(33.33%),前者LCD发生率明显高于后者(P<0.05)。结果还发现,HBsAg和HBcAg阴性肝硬化组织中LCD发生率(65.71%;77.78%)明显高于HBsAg和HBcAg阴性的肝硬化及HBsAg、HBcAg阳性的非肝硬化病例(P<0.01,P<<0.05)。表明LCD的发生与肝硬化和HBV均有关。

 
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