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tissue extracts
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  组织提取物
     Prostatic tissue extracts from 20 patients with prostatic carcinoma (PC) and 20 patients with benign prostatic hyperplasia (BPH) were quantitatively assayed for the expression of transforming growth factor β 2 (TGF β 2) by using sandwich ELISA.
     采用双抗体夹心法 EL ISA测定了 2 0例前列腺癌和 2 0例前列腺增生 (BPH)患者前列腺组织提取物中转化性生长因子 β2 (TGF- β2 )的含量。
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     The patients'sera recognized the 210kd and 190kd antigens from human keratinocyte extracts and lung tissue extracts.
     他们的免疫学表现较为一致 ,血清中抗体可识别人角质形成细胞及边缘肺组织提取物中的 2 10kD和 190kD抗原。
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     In in vitro chemoinvasion assay that different tissue extracts from mice were used to induce human hepatocellul ar carcinoma cells with different metastatic potentials, lung extracts show the strongest inducing activity.
     在小鼠不同组织粗提物诱导高转移潜能的人原发性肝癌细胞的体外趋化侵袭实验中,肺组织提取物具有最强的诱导能力。
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  组织浸液
     Detection of thrombomodulin in both plasma and tissue extracts of cancer patients and its clinical significance
     癌症患者血浆和组织浸液凝血酶调节蛋白的检测及临床意义
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     Methods The level of t-PA, u-PA and TM in cancer tissue extracts including their adjacent normal tissue in 42 cases of lung cancer patients were determined by ELISA.
     方法用ELISA法检测42例肺癌患者癌组织及其邻近正常组织浸液和手术前后血浆中TM、u-PA和t-PA浓度。
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     The TM levels in cancer tissue extracts were significantly lower than that in their adjacent normal tissue extracts[(647.71±317.51)μg/L, vs (1455.63±772.22)μg/L, P <0.01]. On the contrary, the plasma TM levels in these cancers were higher than that in controls.
     癌组织浸液 TM[(6 47.71± 317.5 1) μg/ L]显著低于其邻近正常组织 [(14 5 5 .6 3± 772 .2 2 ) μg/ L,P<0 .0 1],而其血浆 TM则明显高于对照组。
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     Expression of hepatoma associated antigen, HAg18-1, was examined in the sera and tissue extracts of patients with primary hepatocellular carcinoma (PHC) and patients with other malignant tumors as well as in healthy subjects using HAgl8-1 ELISA Diagnostic Kit. The results showed that the positivity was 80.0-86.0%, 44.2-76.2% and 62.0% in PHC patients, patients with other malignant tumors and healthy subjects, respectively.
     本文报告用HAg18-1 ELISA诊断药盒测定肝细胞肝癌病人,胃、肠、肺、乳腺等多种恶性肿瘤病人血清及对肿瘤组织浸液的HAg18-1阳性率,其数值分别为80.0%~86.1%、44.2%~76.2%和62.0%。
短句来源
     The genotoxin and antimutation of spleen tissue extracts were detected by theAmes and SOS colorimetric test. Salmonells typhimurium TA98,TA100 were selected as theAmes tester strain. Escherichia coli k 12 PQ37 was used in the SOS chromotest.
     采用大肠杆菌PQ37的SOS显色法和TA100、TA98菌株的Ames试验检测动物碑组织浸液的遗传毒性。
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  组织提取液
     HACs were cultured in RPMI1640 medium(1640), RPMI1640 and normal brain tissue extracts(1640+NB), RPMI1640 and traumatic brain tissue extracts(1640+TBI).
     将HACs分别接种于RPMI1640培养基(1640)、RPMI1640 培养基与正常脑组织提取液(1640+NB)、RPMI1640 培养基与创伤性脑组织提取液(1640+TBI)3 组。
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     So we explored the differentation and proliferation of human amniotic cells which culture conditioned by traumatic brain tissue extracts to simulate the microenvironment of injured brain,and evaluated the effects after transplanting HACs and GDNF-expressing HACs on the motor function and hippocampal neuron following TBI in rats.
     本实验拟将HACs 置于创伤性脑组织提取液的条件培养基中探究HACs 在体外模拟创伤性脑损伤微环境下的分化和增殖情况,并用HACs 和表达胶源性神经生长因子(glial cell line-derived neurotrophic factor, GDNF)的HACs 靶点移植于大鼠TBI 模型以探究二者分别对TBI 大鼠运动功能和海马神经元的影响。
短句来源
     A rat model of traumatic brain injury was established by Feeney techniques,and the traumatic brain tissue extracts was made 24 hours post-TBI.
     采用Feeney 法制作大鼠TBI 模型,TBI24h后制备创伤性脑组织提取液
短句来源
     The biodistribution and metabolismof ̄3H-gastrodigenin and  ̄3H-gastrodin after intra-venous injection were studied by the detection of theirradioactivity in mice tissue; the radioactive elements ofmice tissue extracts after intravenous injection of  ̄3H-gastrodin were analyzed with thin-layer chromatogra-nhy(TLC).
     作者通过静脉给药探测 ̄3H-天麻甙元和 ̄3H-天麻素在小鼠体内的分布,并采用硅胶薄板层析法对注射 ̄3H-天麻素的小鼠组织提取液的放射性成份进行分析,研究了二者在小鼠体内的分布和代谢过程。
短句来源
     ConclusionHomologous adipose tissue extracts can accelerate velocity and improve the quality of wound healing after skin injury in rats.
     结论同种异体脂肪组织提取液可加快大鼠皮肤创面愈合的速度,改善愈合质量。
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  “tissue extracts”译为未确定词的双语例句
     Hb AGE significently positively correlated with glomerular tissue extracts AGE (GTE AGE) (r=0.6862, P<0.01), and both of them were significantly related to urine protein/creatinine ratio and GBMT.
     Hb AGE与肾小球组织AGE(GTE AGE)含量呈显著性正相关 (r=0 .6 86 2 ,P <0 .0 1) ,二者与尿Pr/Cr比值和GBMT具显著相关性 ;
短句来源
     3) There was the direct correlations between VEGF expression, VEGF concentration in tissue extracts or serum VEGF level (P<0.05).
     3)癌组织VEGF表达、组织和血清VEGF水平间具有密切相关性(P<0.05);
短句来源
     MethodsThe mRNA expression of sialyltransferases, including ST3Gal-Ⅲ and ST6Gal-Ⅰ, was detected in the tissue extracts of 35 cases with bile duct carcinoma(BDC) and 35 cases of benign biliary disease (BBD) by semiquantitative reverse transcription-polymerase chain reaction(RT-PCR).
     方法采用半定量RT PCR法 ,分析 35例胆管癌和 35例良性胆管组织唾液酸转移酶ST6Gal Ⅰ和ST3Gal ⅢmRNA的表达 ;
短句来源
     The recovery rate of betaine in tissue extracts range d from 90% to 98%.
     此色谱条件能使甜菜碱与其它组分很好的分离,甜菜碱加样回收率达到90 %~98% .
短句来源
     Effects of Brain Tissue Extracts of Hypoxia Preconditioned Mice on PC12 Cells
     缺氧预适应小鼠脑匀浆提取液对PC12细胞的影响
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  tissue extracts
We studied the interaction between O,O-diethyl-S-[(N-acyl-N-alkoxycarbonylalkyl)aminomethyl]thiophosphates and mammalian cholinesterases as well as esterases from insect tissue extracts by kinetic methods and disc electrophoresis.
      
Fucoidan hydrolase activity was assessed by examining the effect of animal tissue extracts on fucoidans from the two brown seaweeds Laminaria cichorioides and Fucus evanescens, which have different structural characteristics.
      
The effectors of cGMP metabolism, guanylin, protoporphyrin IX, and zaprinast, elevated the cGMP content in tissue extracts from oat seedlings.
      
It was found that the rate of lipolysis was enhanced during ischemia/anoxia while the lipase activities in tissue extracts involved in the myocardial lipolysis and the amount of triglycerides were not affected.
      
Analysis of tissue extracts by31P NMR and ion chromatography indicated that the most prominent components of the phosphomonoesters were glucose 6-phosphate, α-glycerol phosphate and AMP.
      
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It has been found, that the extracts of different tissues (liver, spleen, muscles, kidney), made according to Filatov, are capable of stimulating adrenal cortex as judged by their ability to cause a decline in adrenal ascorbic acid after intraperitoneal administration into albino rats. But this adrenocorticotropic action requires the presence of an intact pituitary gland, that is to say, all the tissue extracts examined, contain an ACTH releasing factor, which stimulates the release of ACTH from anterior...

It has been found, that the extracts of different tissues (liver, spleen, muscles, kidney), made according to Filatov, are capable of stimulating adrenal cortex as judged by their ability to cause a decline in adrenal ascorbic acid after intraperitoneal administration into albino rats. But this adrenocorticotropic action requires the presence of an intact pituitary gland, that is to say, all the tissue extracts examined, contain an ACTH releasing factor, which stimulates the release of ACTH from anterior pituitary. It has been found, that the ACTH releasing factor is ultrafiltrable. The possible role of this factor in the regulation of anterior pituitary secretion of ACTH in stress and in clinical tissue-therapy is discussed.

以腎上腺抗壞血酸降低法證明不同的組織浸液(肝、脾、腎、肌)均具有興奮腎上腺皮質的作用,但此作用是通過腦垂體前葉ACTH的分泌而發揮的,在割出腦垂體的動物組織浸液即不再呈現作用。由於此組織浸液所含有的有效成份可以透過超瀘膜,故其可能為小分子的代謝產物。最後並將此實驗的結果聯系腦垂體前葉ACTH的分泌機制及組織療法的功效加以討論。

The stability of synthetic phosphoramides of aniline,p-chloroaniline,p-amino- benzoic acid,o-methoxyaniline sulfanilamide and benzylamine has been tested separately in acid and alkaline(pH 5.0 and pH 9.0)solutions.It was found that all these compounds are hydrolyzed in different degrees;among which N-(p-chlorophenyl)amidophosphoric acid is the most stable.This compound was thus used as substrate for most enzyme studies in spite of its low solubility.Phosphoramides of p-aminobenzoic acid and benzylamine are unsuitable...

The stability of synthetic phosphoramides of aniline,p-chloroaniline,p-amino- benzoic acid,o-methoxyaniline sulfanilamide and benzylamine has been tested separately in acid and alkaline(pH 5.0 and pH 9.0)solutions.It was found that all these compounds are hydrolyzed in different degrees;among which N-(p-chlorophenyl)amidophosphoric acid is the most stable.This compound was thus used as substrate for most enzyme studies in spite of its low solubility.Phosphoramides of p-aminobenzoic acid and benzylamine are unsuitable as substrates,due to their instability in solution in which the enzyme activity is measured. The phosphoamidase aetivity of the purified enzyme preparation from ox spleen on the phos- phoramide compounds is higher at pH 5.0 than at pH 9.0.The rate of hydrolysis and the affinity of the enzyme with the substrate are different for different phosphoramides.It is therefore evident that phosphoamidase activity is closely related to the structure of the substrate. Among the splitting products of phosphoramides,amines have little effect on the enzyme activity,while phosphate ion has quite significant inhibitory effect. From the experiments on the enzyme activity of rat tissue ertracts,it is seen that: (1)The ratio.of the activities on substrates with similar structures is parallel in all tissues, but different in substrates with different structures,indicating that there may exist more than one phosphoamidases. (2)The content of phosphoamidase in the various tissues of the rat varies to a considerable extent.Spleen has the highest phosphoamidase content. (3)Muscle-tissue extract contains a considerable amount of phosphoamidase activity upon synthetic compounds containing P-N linkage.It would appear that phosphocreatine,which is rich in muscle,might be the natural substrate for this enzyme.

(一)磷酰胺类化合物在酸性及碱性溶液中都不稳定。在所试过的化合物中,以磷酰对氯苯胺最稳定,但它的溶解度较小。磷酰参羧基苯胺和磷酰苯甲胺的自行分解速度甚大,不过宜用为底物。 (二)牛脾部分提纯制剂在 pH5.0对于磷酰胺类化合物的活力比在 pH9.0的高。而对于不同底物的水解速度与亲和力都不相同,显示磷酸氨基酶活力与底物的结构有密切关系。在分解产物中,胺类对于该酶的活力无大影响,而磷酸根离子则有较为显著的抑制作用。(三)大鼠组织提取液对于结构相似的底物的活力之比也相似,而对于结构不同的底物,则活力之比也不相同,显示有不同磷酸氨基酶存在的可能。(四)各组织中磷酸氨基酶的含量不同,以脾脏的含量最高。(五)肌肉组织中合有磷酸氨基酶的活力,它的研究可能对于肌肉中磷酸肌酸代谢的了解有所帮助。

Qualitative analysis of the tissue extracts of Philosamia cynthia ricini showed thatin all stages of development the soluble proteins contained albumins, globulins and gela-tin. In the beginning of the pupal stage, primary proteose and peptone were also de-tected in the tissue extract. The quantities of albumins and the soluble proteins ingeneral, determined with the Folin phenol reagent, were found to be variable in differentstages of development. They were highest in the newly formed pupae, and...

Qualitative analysis of the tissue extracts of Philosamia cynthia ricini showed thatin all stages of development the soluble proteins contained albumins, globulins and gela-tin. In the beginning of the pupal stage, primary proteose and peptone were also de-tected in the tissue extract. The quantities of albumins and the soluble proteins ingeneral, determined with the Folin phenol reagent, were found to be variable in differentstages of development. They were highest in the newly formed pupae, and decreasedgreatly toward the end of pupal stage while the imaginal organs were being formed.The amount of globulins, however, remained nearly constant throughout all the develop-mental stages. Gelatin, which gives a negative reaction with the Folin phenol reagent,was noted to be present in abundance in the mature larvae. Sexual difference wasobserved in the soluble protein content. It was most remarkable in the mature pupaeand the adults, showing apparent relation to the egg formation in the females. The amino acid composition of the soluble proteins was studied by means of paperchromatography. The following common amino acids were detected: cystine (and/orcysteine), lysine, histidine, arginine, serine, glycine, aspartic acid, glutamic acid, threonine,alanine, proline, tyrosine, valine (and/or methionine), phenylalanine, leucine (and/or iso-leucine). Tryptophan could not be found on the chromatograms due to decomposition inthe process of acid hydrolysis. In the pupal and imaginal stage, the percentage contentsof glycine, alanine, tyrosine and serine were lower as compared with those in the larvaeimmediately before spinning. The physiological significance of the developmental and sexual differences in thecontents of the soluble proteins has been discussed.

水文研究了不同发育阶段蓖麻蚕组织中可溶性蛋白质在性质及含量上的变化。在各发育阶段可溶性蛋白质都包含有清蛋白类、球蛋白类及明胶。在化蛹初期,组织提取液中还发现了初级(月示)和胨。除明胶以外的可溶性蛋白质总量及其中清蛋白类的含量因发育阶段而异:在蛹初期最高,到羽化前后有显著下降,球蛋白类的含量在各发育阶段比较稳定。雌雄两性的可溶性蛋白质含量不同,在羽化前后这种两性差异最为显著,雌性超过雄性约一倍之多。因此,在蛹后期及成虫中,蛋白质的两性差异在性质上比在数量上更为显著。 曾用纸上层析法分析了可溶性蛋白质的氨基酸组分。在各发育阶段,氨基酸的种类相同,包括胱氨酸(和半胱氨酸)、赖氨酸、组氨酸、精氨酸、丝氨酸、甘氨酸、天门冬氨酸、谷氨酸、苏氨酸、丙氨酸、脯氨酸、酪氨酸、缬氨酸(和蛋氨酸)、苯丙氨酸、以及亮氨酸(和异亮氨酸)。色氨酸在酸水解过程中被破坏。文中列出不同发育阶段可溶性蛋白质中各种氨基酸的百分含量。老熟幼虫吐丝结茧后,甘氨酸、丙氨酸、酪氨酸和丝氨酸含量下降。

 
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