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the optimal ph     
相关语句
  最适ph
     ③the optimal pH value is between 3.0~5.5;
     ③最适pH在3.0~5.5之间。
短句来源
     the suitable pH was ranged from 4.0~8.0, the optimal pH was 6.0~7.0;
     适宜 pH为 4 .0~ 8.0 ,最适pH为 6 .0~ 7.0 ;
短句来源
     the optimal pH for K1 was 8,and 7 for K2 and K3,respectively.
     K1菌最适pH为8,K2、K3菌最适pH为7;
短句来源
     Results The optimal pH was 8.6 and optimal substrate concentration was 2.0 mmol/L.
     结果最适pH值为8.6,基质浓度为2.0mmol/L。
短句来源
     the optimal pH was 6-7;
     最适pH6~7;
短句来源
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  最适ph值
     Results The optimal pH was 8.6 and optimal substrate concentration was 2.0 mmol/L.
     结果最适pH值为8.6,基质浓度为2.0mmol/L。
短句来源
     The optimal pH and temperature for laccase were pH4 0 and 30℃ respectively.
     漆酶最适pH值为 4 0 ,最适反应温度为 30℃。
短句来源
     Results showed that the optimal pH values of proteolytic enzyme of the crab were 5.4—5.8 and 7.2—7.6. The optimal substrate consistency of casein were: stomach, 0.8 mg/ml;
     研究结果表明:河蟹消化道蛋白酶的最适pH值在5.4~5.8和7.2~7.6; 蛋白酶的最适底物酪蛋白的浓度为:胃,0.8mg/ml;
短句来源
     (3)The molecular mass of PE was 48.8kDa,the optimal pH and temperature was 5.8 and 60℃ respectively.
     (3)PE分子量为48.8kDa,最适pH值为5.8,最适温度为60℃。
短句来源
     (4)The molecular mass of PG was 71.5kDa, the optimal pH and temperature was 3.0 and 65℃ respectively.
     (4)PG分子量为71.5kDa,最适pH值为3.0,最适反应温度为65℃。
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  最佳ph
     The optimal pH, T, HRT and C/N ratio were 5.0、33.5-36.5℃、8.34 h、112/1.55, respectively.
     最佳pH、T、HRT、C/N比分别是5.0、33.5~36.5℃、8.34 h、 112/1.55。
     The results of the study showed that:when the initial concentration of As(Ⅴ)or As(Ⅲ)was 0.1 mmol/L,the optimal pH value for As(Ⅴ)and As(Ⅲ)removal were 4-8 and 6-9 respectively;
     研究结果表明,在初始As(Ⅴ)或As(Ⅲ)浓度为0.1mmol/L条件下,去除As(Ⅴ)的最佳pH值为4~8,去除As(Ⅲ)最佳pH值为6~9;
短句来源
     Protoplasts were separated from mesophyll cells of primula obcouica under different pH conditions(5.2, 5.4, 5.6, 5.8, 6.0, 6.2, 6.4and 6.6)with cellulase(made in China). The results showed that the optimal pH is 5.6.
     用国产纤维素酶分离四季樱草叶肉细胞原生质体,分别在八种pH值(5.2,5.4,5.6,5.8,6.0,6.2,6.4和6.6)下进行酶处理试验,得出5.6是最佳pH值的结果。
短句来源
     The optimal pH value of chelation was 3. The optimal process conditions of H_2O_2 bleaching were: pulp consistency 10%, H_2O_2 charge 3.5%, NaOH charge1.5% (wheat straw), 1.6% (bagasse), MgSO_4 0.05%, 90°C and 4h.
     螯合处理的最佳pH值为3。 H_2O_2漂白的最佳工艺条件为:浆浓10%,H_2O_23.5%,NaOH 1.5%(麦草浆),1.6%(蔗渣浆),MgSO_40.05%,90℃,时间4h。
短句来源
     At pH level:2.0~6.0, Adsorption capacity of vermiculite increase with pH growing, and the optimal pH value:4.0~(6.0);
     蛭石吸附量在pH2.0~6.0范围内随着pH的增大而增大,最佳pH为4.0~6.0;
短句来源
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  最佳ph值
     The results of the study showed that:when the initial concentration of As(Ⅴ)or As(Ⅲ)was 0.1 mmol/L,the optimal pH value for As(Ⅴ)and As(Ⅲ)removal were 4-8 and 6-9 respectively;
     研究结果表明,在初始As(Ⅴ)或As(Ⅲ)浓度为0.1mmol/L条件下,去除As(Ⅴ)的最佳pH值为4~8,去除As(Ⅲ)最佳pH值为6~9;
短句来源
     Protoplasts were separated from mesophyll cells of primula obcouica under different pH conditions(5.2, 5.4, 5.6, 5.8, 6.0, 6.2, 6.4and 6.6)with cellulase(made in China). The results showed that the optimal pH is 5.6.
     用国产纤维素酶分离四季樱草叶肉细胞原生质体,分别在八种pH值(5.2,5.4,5.6,5.8,6.0,6.2,6.4和6.6)下进行酶处理试验,得出5.6是最佳pH值的结果。
短句来源
     The optimal pH value of chelation was 3. The optimal process conditions of H_2O_2 bleaching were: pulp consistency 10%, H_2O_2 charge 3.5%, NaOH charge1.5% (wheat straw), 1.6% (bagasse), MgSO_4 0.05%, 90°C and 4h.
     螯合处理的最佳pH值为3。 H_2O_2漂白的最佳工艺条件为:浆浓10%,H_2O_23.5%,NaOH 1.5%(麦草浆),1.6%(蔗渣浆),MgSO_40.05%,90℃,时间4h。
短句来源
     When it was used for the optical resolution of N-acetyl-DL-methionine,the optimal pH,temperature and the concentration of Co2+ were 6.5,65℃ and 5×10-4mol/L respectively.
     结果表明,最佳pH值、温度和Co2+浓度分别为6.5、65℃和5×10-4mol/L。
短句来源
     The optimal pH range values are 8~9 and 7~9 respectively during the processes of nitrification and denitrification.
     硝化过程的最佳pH 值范围为8~9,反硝化过程的最佳pH 值是7~9。
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      the optimal ph
    The optimal pH was 8.5-9.5 for hydrogen uptake and 4.0 for H2 evolution.
          
    The Km values of recombinant DABAcT measured at the optimal pH and temperature in the presence of 0.2 M KCl were 460 and 36.6 μM for L-2,4-DAB and acetyl-CoA, respectively.
          
    sulfidophilum andHbt undosum is 30-35?C, and the optimal pH is 7-8.
          
    With TMPD as an electron donor for the cbo-type oxidase, the optimal pH (7.0 to 7.6) was determined from the difference between respiration rates in the presence of ascorbate/TMPD and only ascorbate.
          
    The optimal growth temperature is 25-28°C; the optimal pH is 6.8.
          
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    In phosphate buffer the optimal pH for the activity of the particle-bound CoIH cytochrome c reductase system of heart muscle preparation is between pH 7 and 8, whereas in glycylglycine buffer it is pH 8.0. The activity of this enzyme system in the latter buffer is considerably lower than that in the former (Fig. 1). Addition of ethylenediamine tetraacetic acid caused considerable stimulation of its activity in glycylglycine buffer, indicating that the lowered activity in the latter buffer is...

    In phosphate buffer the optimal pH for the activity of the particle-bound CoIH cytochrome c reductase system of heart muscle preparation is between pH 7 and 8, whereas in glycylglycine buffer it is pH 8.0. The activity of this enzyme system in the latter buffer is considerably lower than that in the former (Fig. 1). Addition of ethylenediamine tetraacetic acid caused considerable stimulation of its activity in glycylglycine buffer, indicating that the lowered activity in the latter buffer is probably due to disturbance in the physical state of the enzyme preparation. The optimal pH for the particle-bound CoIH cytochrome c reductase system of heart muscle preparation, whether in phosphate or in glycylglycine buffer, differs markedly from the value of 8.7 reported by Mahler et al for their soluble CoIH cytochrome e reductase in glycylglycine buffer. It has been found that addition of a relatively large amount of the soluble CoIH cytochrome c reductase to heart muscle preparation does not result in any increase in activity of the CoIH oxidase system. The amount of soluble CoIH cytochrome c reductase added had an activity about 8 times that of the reductase system already present. The addition of this amount of the soluble enzyme also fails to restore to any appreciable extent the CoIH oxidase activity of heart muscle preparations previously treated with 2,3-dimercaptopropanol. It is known that 2, 3-dimercaptopropanol treatment does not affect cytochrome c or cytochrome oxidase. These show that the soluble CoIH cytochrome c reductase of IV[abler et al reacts only with soluble cytochrome c but not with the cytochrome c firmly bound to the particulate matter of enzyme preparations. The cytochrome c reductase activity of the Keilin-Hartree heart muscle preparation can be easily extracted by 9% alcohol at pH 5.4 using the procedure of Mahler et al. However even when the CoIH cytochrome c reductase activity of the heart muscle preparation has been completely destroyed by 2, 3-dimercaptopropanol treatment, the alcohol extract still contains the soluble reductase with an activity comparable to that extracted from an untreated control (Table 5). This seems to indicate that the soluble reductase of Mahler et al is formed during the extraction procedure and is therefore different from the enzyme originally present in the heart muscle preparation. The evidence presented above together with those previously obtained by us and by other workers all point to the conclusion that the soluble reductase of Mahler et al is an artifact.

    (一)心肌上的輔酶Ⅰ細胞色素c還原酶系活力的最適pH和水溶性輔酶Ⅰ細胞色素c還原酶活力的最適pH顯著不同,酶系物理狀態對酶活力影響頗大。 (二)水溶性輔酶Ⅰ細胞色素c還原酶不能和心肌製劑顆粒上的細胞色素c作用。心肌製劑在經過[2,3]二氫硫基丙醇處理完全破壞原有輔酶Ⅰ細胞色素c還原酶系活力以後仍能抽提出活力很強的水溶性輔酶Ⅰ細胞色素c還原酶。這些以及我們過去曾經討論過的一些事實都說明Mahler等所獲得的水溶性輔酶Ⅰ细胞色素C還原酶是一個矯作物。 (三)本研究指出根據用人為的方法從複雜酶系中抽出的酶的性質簡單地判斷該酶在整個酶系中的作用不一定是可靠的。

    Using the technique of paper chromatography, we have investigated the transaminationreactions between twenty-two different amino acids and α-ketoglutaric acid in the homogenate ofSchistosoma japonicum. The formation of glutamic acid was demonstrated with alanine, arginineand aspartic acid as substrates. The activities of glutamic-pyruvic transaminase (GPT) and glutamic-oxaloacetic transaminase(GOT) in the homogenates of the worms were determined. For paired worms, the averagevalues found were in micromol./mg.N/hour,...

    Using the technique of paper chromatography, we have investigated the transaminationreactions between twenty-two different amino acids and α-ketoglutaric acid in the homogenate ofSchistosoma japonicum. The formation of glutamic acid was demonstrated with alanine, arginineand aspartic acid as substrates. The activities of glutamic-pyruvic transaminase (GPT) and glutamic-oxaloacetic transaminase(GOT) in the homogenates of the worms were determined. For paired worms, the averagevalues found were in micromol./mg.N/hour, 20.1 and 16.9 for GPT and GOT respectively.When estimated separately, the activities for female worms were found to be higher than those ofthe male worms. The optimal pH for both enzymes was 7.2-7.5; the optimal substrate concentrations werefound to be 0.02 M for α-ketoglutaric acid, and 0.2-0.4M for both DL-alanine and DL-asparticacid. Tartar emetic, Sb-58 and Fouadin had a notable inhibitory effect on GPT of S. japonicum.It was shown that both the forward and the reverse reactions were inhibited 50% by 10~(-4)Mtartar emetic. This inhibitory effect could be reversed by the addition of sodium dimercaptosuc-cinate. All these three compounds did not show any inhibitory effect on GPT in the mouse liver,but acted on the enzyme of Fasciola hepatica in a similar way as on that of S. japonicum. TheGOT of S. japonicum was inhibited only by Sb-58 but not by the other two antimonial drugs.

    用纸上层析法测定了日本血吸虫匀浆对22种氨基酸与α-酮戊二酸的转氨作用,当用丙氨酸、精氨酸及天门冬氨酸作底物时,可明显地测出谷氨酸的生成。日本血吸虫的谷氨酸-两酮酸转氨酶(谷丙酶)及谷氨酸-草酰乙酸转氨酶(谷草酶)的活力经用比色法多次测定,所得结果波动不大,雌雄合抱虫活力的平均值(微克分子/毫克氮量/60分)谷丙酶为20.1,谷草酶为16.9。在雌雄虫分别测定中,雌虫的酶活力较高。二种酶的最适pH均为7.2—7.5,底物最适浓度α-酮戊二酸为0.02M,DL-丙氨酸及DL-天门冬氨酸同为0.2-0.4M。吐酒石、Sb-58及(月弟)芬对日本血吸虫的谷丙酶有明显的抑制作用,当吐酒石的浓度为10~(-4)M时正逆二个方向的反应均被抑制约5O%,这种抑制作用能被二巯基丁二酸钠所解除。锑剂对小白鼠肝脏的谷丙酶无作用,但对肝吸虫的作用与血吸虫相似。血吸虫的谷草酶亦能被Sb-58所抑制,但不受其他二种锑剂的影响。

    The phenolase of Schistosoma japonicum has been studied with a manometric method. The homogenate of the female worm could oxidize different phenolic compounds. When L-tyrosine, 3,4-dihydroxyphenylalanine, p-cresol and o-catecol were used as substrates, the enzymic activity(Q_(O_2)=μl/mg N/hr) of the paired worm at pH 6.8 was 5.5, 10.4, 11.3 and 3.2 respectively. Owing to the formation of pigment, the colour of the medium turned gray or brown. The male worm did not exhibit this enzymic activity. When tyrosine...

    The phenolase of Schistosoma japonicum has been studied with a manometric method. The homogenate of the female worm could oxidize different phenolic compounds. When L-tyrosine, 3,4-dihydroxyphenylalanine, p-cresol and o-catecol were used as substrates, the enzymic activity(Q_(O_2)=μl/mg N/hr) of the paired worm at pH 6.8 was 5.5, 10.4, 11.3 and 3.2 respectively. Owing to the formation of pigment, the colour of the medium turned gray or brown. The male worm did not exhibit this enzymic activity. When tyrosine was used as substrate, at a concentration of 4mM, the oxygen uptake approached maximum, the optimal pH being in the range of 8.0-8.8. In vitro, tartar emetic at a final concentration of 10~(-4)M, caused 60% inhibition of the enzymic activity. This inhibitory effect could be also demonstrated in vivo. The phenolase activity of the worms obtained from infected guinea pigs which had been treated intraperitoneously with tartar emetic(20mg/kg) one hour before sacrifice, was 40-70% lower than that of the untreated control. The phenolase was also present in Fasciola hepatica, but it differed from that of Schistosoma japonicum in substrate specificity, since it oxidized o-catecol much faster than tyrosine.

    日本血吸虫成虫取自人工感染的豚鼠,磨成匀浆后用Warburg呼吸仪測定其酚酶活力。在pH6.8时,对所試4种酚类均有显著的氧化作用,耗氧量以对甲酚为最強,其次为3,4-二羟苯丙氨酸和酪氨酸,邻苯二酚最弱。酶作用后反应液呈灰黑色(3,4-二徑苯丙氨酸,酪氨酸)或赭紅色(对甲酚,邻苯二酚),氧耗越強,色泽越深。以酪氨酸作为底物在pH8.0,最后浓度为4mM时酶活力已接近最高峯;在所試的pH范围(6.5—8.8)內,酶活力随着pH的上升而增高,在pH 8.0—8.8时活力无大差別。肝片吸虫中亦存在着酚酶,但对上述四种底物的相对活力与血吸虫不同,对邻苯二酚和对甲酚的活力远較3,4-二羟苯丙氨酸和酪氨酸为高。血吸虫的酚酶仅存在于雌虫中,雄虫匀浆无此酶作用。治疗血吸虫病的有效药物酒石酸銻鉀对血吸虫的酚酶有显著的抑制作用,最后浓度为10~(-4)M时,酶活力被抑制約60%。以20毫克/公斤体重的酒石酸銻鉀注射感染血吸虫的豚鼠,1小时后解剖取得血吸虫,其酚酶活力較不注射对照低40—70%。由于酚酶在吸虫产卵功能中的重要性,这种抑制作用亦可部分地解释酒石酸銻鉀的作用机制。

     
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