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gene-transfer
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  “gene-transfer”译为未确定词的双语例句
     Mammalian Gene-transfer and Expression Efficiencies of Baculovirus BacV-CMV-EGFPA
     杆状病毒对不同哺乳动物细胞基因转移及表达效率的研究
短句来源
     In this study, we examined the expression of caspase-6 in the osteosarcomaline SOSP-9067 by RT-PCR method. Results showed no expression of caspase-6.We constructed a caspase 6 gene-transfer virus adv5 vectors and transfeced it intothe osteosarcoma line SOSP-9607 by way of lipofection.
     本实验应用 RT—PCR方法检测了成骨肉瘤细胞株 SOSP—9607 中Caspase-6的表达,发现其无表达。
短句来源
     Heat shock pretreatment(45℃,10min)increased gene-transfer efficiency.
     45℃热激预处理原生质体有效地促进了CAT 基因的电击转移。
短句来源
     Gene-transfer efficiency is evaluated by a detection of GUS activity resulting from the chimeric plasmid DNA.
     以报告基因GUS酶活性为指标,借以测定转化了的水稻细胞。
短句来源
     A caspase-6 gene-transfer virus adv 5 vectors was constructed and transfeced into the GRC-1 line by way of lipofection. The cell survival rate after transfection was assayed by MTT method.
     以腺病毒 adv5作载体 ,构建了含 Caspase- 6基因的转基因载体 ,用脂质体包裹法转染 GRC- 1细胞株 ,用 RT-PCR方法检测转染后 GRC- 1细胞中 Caspase- 6基因的表达。
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  相似匹配句对
     OBESE GENE
     肥胖基因
短句来源
     Rescue Gene
     抢救植物基因
短句来源
     -casein gene.
     -酪蛋白基因5'侧序列。
短句来源
     Transfer Gene Animals
     论转基因动物
短句来源
     "Gene-trapping" Technique.
     基因捕获技术
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  gene-transfer
We also summarize the results obtained with electro-gene-transfer in animal models to date and the technical issues that must be solved before its use for human therapy can be considered.
      
This review provides a brief overview of the theory of electro-gene-transfer and describes parameters governing its efficiency in muscle.
      
Electro-gene-transfer increases gene expression by several orders of magnitude and strongly reduces interindividual variability.
      
Electro-Gene-Transfer: A New Approach for Muscle Gene Delivery
      
M19 cells reverted to lipid prototrophy at very low frequency and were chosen as recipients to perform DNA-mediated gene-transfer experiments using total human genomic DNAs.
      
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With Electric-pulse mediated gene transfer method, plasmid PBCI of B. circulans 3342, which is Butirosin producing strain, can be transfereddinto E. coli C_(600). The results showed that the auxotrophic properties of fusion products are just the same as E.coli C_(600).but the plasmid DNA is distinguishable from the plasmid PBCI. The reason for the changes are not clear.

通过电击基因转移的方法,丁酰苷菌素产生菌环状芽孢杆菌3342中的质粒PBCI可以转移进入大肠杆菌,结果表明融合子营养缺陷型性状与大肠杆菌C600相同,但质粒DNA与PBCIDNA有所不同,原因尚不清楚。

A cDNA of a human mKNA coding for thymidine kinase was isolated from a human fibroblast cDNA library. The size of cloned TK cDNA (TKhc9) was measured to be 1.37 kb. The restriction sites-map of TKhc9 was defined. This TK cDNA can not express TK activity after it has entered the mouse TK deficient cell line Ltk- cells through the DNA-mediated gene transfer because it misses about 160 base pairs coding sequence. However, this near full-length TK cDNA can transform the Ltk- cells to acquire TK phenotype if it cotransfects...

A cDNA of a human mKNA coding for thymidine kinase was isolated from a human fibroblast cDNA library. The size of cloned TK cDNA (TKhc9) was measured to be 1.37 kb. The restriction sites-map of TKhc9 was defined. This TK cDNA can not express TK activity after it has entered the mouse TK deficient cell line Ltk- cells through the DNA-mediated gene transfer because it misses about 160 base pairs coding sequence. However, this near full-length TK cDNA can transform the Ltk- cells to acquire TK phenotype if it cotransfects with a 4kb HindⅢ/KpnI DNA fragment isolated from human genomic thymidine kinase gene. By using the Southern blot analysis and isoenzyme assay, the human cDNA cloned was identified.

本文报道了从人体成纤维细胞mRNA制成的cDNA文库中分离出细胞质胸腺嘧啶核苷激酶(TK-C;EC2.7.1.21)的cDNA。测定了它的大小约为1.37kb,确定了它的酶切图谱。这是缺失了将近160个核苷酸对的非全长的人体TK-C cDNA。它单独不能表达TK活性。可是当它同另一个单独也不产生TK活性的、从人体TK-C基因中分离出来的长为4kb的DNA片段一起转化小鼠TK缺陷型细胞Ltk~-时,却能通过某种方式重组成一个有功能的单位,从而产生了TK,使Ltk~-细胞获得了TK表型。

A fragment of human cytosolic thymidine kinase (TK-C) gene and a fragment of human TK cDNA were introduced into thymidine kmase-deficient mouse L cells by DMA-mediated gene transfer. Southern blot analyses demonstrated that these two fragments have reconstructed as an intact functional unit expressing TK activity. The mechanism involved may be the homologous recombination. The stability of thymidine kinase-producing transformants was analyzed. All transformants studied express stably TK. The fate of exogenous...

A fragment of human cytosolic thymidine kinase (TK-C) gene and a fragment of human TK cDNA were introduced into thymidine kmase-deficient mouse L cells by DMA-mediated gene transfer. Southern blot analyses demonstrated that these two fragments have reconstructed as an intact functional unit expressing TK activity. The mechanism involved may be the homologous recombination. The stability of thymidine kinase-producing transformants was analyzed. All transformants studied express stably TK. The fate of exogenous DNA fragments in host cells was discussed.

本文报道了人体细胞质胸腺嘧啶核苷激酶(TK-C;EC2.7.1.21)基因的一个片段同TKcDNA的一个片段,一起转化小鼠TK缺陷型细胞株Ltk~-细胞时,这两个单独都不能表达TK活性的DNA片段可能通过同源重组,重新构建成一个能够表达TK的完整的功能单位。分析了TK~+并发转化细胞表达TK的稳定性,并用Southern印迹法杂交证实这两个外源DNA片段已重组成一个片段而整合在受体细胞基因组里,因而并发转化细胞全是稳定型。讨论了两个功能上可以互补的外源DNA片段,在受体细胞里重组成一个能表达基因活性的功能单位的可能机制。

 
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