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  expression ratio
Furthermore, Bax and Bcl-2 positive cells were all increased over time in the operation group, and the expression ratio of Bax/Bcl-2 in the operation group was significantly higher than that in the false operation group (P >amp;lt; 0.01).
      
In conclusion, rCBF decrease can impair the learning and memory function in rats, which may be related to the increase of the expression ratio of c-fos or c-jun or Bcl-2 or Bax in the frontal cortex and hippocampus.
      
The expression ratio (ER) of HCR2 between the tumor and paired tumor-free tissues was calculated for each case and the data was clinicopathologically analyzed.
      
VEGF positive expression ratio was 84.8% in iNOS positive expression cases, and the ratio was 35.3% in negative cases.
      
The CD68 expression ratio in the 30 intestinal type cases was much lower than that in the 18 gastric type cases (t=4.29, P>amp;lt;0.01).
      
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An expression of ras oncogene protein (p21) was assessed with immunohistochemistry in normal, dysplasia, benign hyperplasia, chronic prostatitis and prostatic carcinomatous tissues. The results showed that the positivity rate of p21 was higher in prostatic carcinoma than that in other prostatic lesions and was markedly correlated to histologic tumor grade. The expression level of p21 was more intentive in prostatic dysplasia surrounding carcinoma than that in simple prostatic dysplasia. It is suggested that...

An expression of ras oncogene protein (p21) was assessed with immunohistochemistry in normal, dysplasia, benign hyperplasia, chronic prostatitis and prostatic carcinomatous tissues. The results showed that the positivity rate of p21 was higher in prostatic carcinoma than that in other prostatic lesions and was markedly correlated to histologic tumor grade. The expression level of p21 was more intentive in prostatic dysplasia surrounding carcinoma than that in simple prostatic dysplasia. It is suggested that overexpression of ras oncogene p21 product may play an important role in progressing from dysplasia to cancer and may be used as a new tumor marker for assessment of biological behavior of prostatic carcinoma.

用抗ras癌基因蛋白质产物p21单克隆抗体SCI-oncogema I,采用常规ABC免疫组织化学方法检测了66例前列腺癌、46例前列腺非典型增生、20例慢性前列腺炎、50例良性前列腺增生和6例正常前列腺组织石蜡标本中p21的表达,结果显示,前列腺癌的阳性表达率明显高于其他几组病变(P<0.01),其阳性表达率随肿瘤组织学分级增高而增高。前列腺癌周的不典型增生组织对p21的反应强度显著高于单纯性不典型增生组织。上述结果说明,ras癌基因产物p21的过量表达,在前列腺非典型增生向前列腺癌的发展过程中起着重要作用,并且可以作为一种新的肿瘤标记物来估计前列腺癌的生物学行为。

An immunohistochemical study of transitional cell carcinoma of the urinary bladder using PC10,a monoclonal antibody against proliferating cell nuclear antigen (PCNA/Cyclin), was performed on tissues routinely processed with formalin fixation and paraffin embedding. Clinical follow-up data and histological grade were taken into consideration of the analysis. There was a significant difference in survival between patients with PCNA index(>76% )and low index(<75%). The result indicated that PCNA/Cyclin determination...

An immunohistochemical study of transitional cell carcinoma of the urinary bladder using PC10,a monoclonal antibody against proliferating cell nuclear antigen (PCNA/Cyclin), was performed on tissues routinely processed with formalin fixation and paraffin embedding. Clinical follow-up data and histological grade were taken into consideration of the analysis. There was a significant difference in survival between patients with PCNA index(>76% )and low index(<75%). The result indicated that PCNA/Cyclin determination is a trustworthy method to examine the fraction of proliferating cells in formalin-fixed, paraffin-embedded section, and might be a new method to assess the prognosis of transitional cell carcinoma.

本文应用抗增殖细胞核抗原/周期素的单克隆抗体PC10,以ABC免疫组织化学方法对49例膀胱移行细胞癌作了研究,并与组织学分级和预后作了对比分析,结果发现:移行细胞癌各级间比较PCNA表达率存在着极显著性差异(P<0.01).PCNA表达强级(>76%)与弱级(<75%)之间比较生存率存在着极显著性差异(P<0.01).结果提示:此方法是在福尔马林固定,石蜡包埋的移行细胞癌切片上检测细胞增殖分数的一个可靠手段,并可能是判断移行细胞癌预后的一个新指标.

Mutation in the gene coding for p53 tumour suppressor protein has been common in a variety of human cancer. To assess the role of a putative mutated p53 protein in human bladder cancer,a monoclonal antibody technigue was used in an immunoperoxidase detection system. A total 50 bladder cancer cases were studide 49 being transitional cell carcinomas and the remaining one squamous cell caricinoma.p53 position nuclear staining was found in 21 of 50 bladder cancer (42%),including 9 of 11 cancer with PT3, 12 of 30...

Mutation in the gene coding for p53 tumour suppressor protein has been common in a variety of human cancer. To assess the role of a putative mutated p53 protein in human bladder cancer,a monoclonal antibody technigue was used in an immunoperoxidase detection system. A total 50 bladder cancer cases were studide 49 being transitional cell carcinomas and the remaining one squamous cell caricinoma.p53 position nuclear staining was found in 21 of 50 bladder cancer (42%),including 9 of 11 cancer with PT3, 12 of 30 cancers with PT2,13 of 17 bladder cancers with Grade 3 and 8 of 25 bladder cancers with grade 2.p53 position nulear staining was not obseved in any of 9 cancers with PTA PT1 and neither in grade 1 cancers 8.A total of 25 patients have been followuped , 10 of 13 cases having p53 position nuclear staining were found to have metasteses(76.9%).The mean survival time was compared between cases, cases with positire,p53 position nucles staining had a statistically significant poor prognostic value.(P<0. 01)

通过免疫组化ABC法,对50例手术切除或活检的膀胱癌标本石蜡切片进行P_(53)基因表达的研究。结果表明:膀胱P_(53)蛋白过度表达率为42%(21/50),其中PT_240%(12/30),PT_382%(9/11),PT_a,PT_1为0;G_232%(8/25),G_372%(13/17),G_1为0。随访25例患者,13例P_(53)阳性过度表达,其中有76%(10/13)有复发转移,而单纯复发者只占23%(3/13)。13例P_(53)阳性表达者平均存活时间22.6个月/例,12例P_(53)阴性表达者平均存活63.7个月/例。P<0.01。

 
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