When four antagonistic fungi (Trichoderma koningii, T. pseudokoningii, T. harzianum and Gliocladium spp. ) were irradiated with ultraviolet light for 30 min, mutants of strains were obtained. Mutants did not change the morphological characters and antagonistic activity to Fuserium axysporum f.
This paper was aimed at screening antagonistic fungi against Agrobacterium tumefaciens from soil. The antagonistic fungi strain was screened by agar column method and the antibacterial activity was determined by filter paper method.
从土壤中筛选出对根癌农杆菌Agrobacterium tum efaciensT-37有拮抗作用的真菌菌株,采用琼脂柱法筛选真菌菌株,滤纸片法测定抑菌活性,结果表明,从土壤中分离纯化出来的52株真菌中首次筛选到1株对根癌农杆菌T-37有抑制作用的拮抗真菌X j-Ⅴ-2,当X j-Ⅴ-2菌株26℃,140 r.
The community structure, distribution pattern, as well as the biological diversity of tree-inhabiting fungi of Pinus sylvestris var. mongolica were investigated, and the antagonistic fungi that have inhibiting effects to the pathogen of shoot blight were screened.
On the basis of ecologic balance, developing the complex biological preparation as a goal, we first explored two compatibly antagonists to control the leaf disease of forest plant and hoped this two antagonistic fungi could play a cooperative role in controlling the pine needle blight.
The main research results were as follows:1 , The dual test showed the two antagonistic fungi(Gliocladiutm virens ,Bacillus firmus)had some differences to Pestalotia funerea in the inhibition mode.
Spraying control wlth scraping for cytospora canker of poplar and selection trials in field have been carried by culturing five antagonistic fungi species selected before on solid medium (PDA) and Czapek' s solution and obtaining spores and fermentation solution of these species, Trichoderma sp. , penicillium sp., Aspergillus sp. , Eurotium so.
Inspection Percentage of Petals Infested about Forecasting Rapeseed Stem Rot、Mycoflora Analysis on Rape Flower and Selection of Antagonistic Fungi to Sclerotinia Sclerotiorum
Spore germiantion of antagonistic fungi (Trichderma viride andAspergillus sydowi) was also influenced by the root exudates of resistant and susceptible varieties, but the influence was different.
Thus, plants may not be able to erect selective barriers to only antagonistic fungi.
Fungal symbiont ranges of hosts supports prediction (2): woody hosts are associated with a greater number of mutualistic fungi than antagonistic fungi.
The potential role of antagonistic fungi in this variability was investigated assessing sensitivity of Fusarium species and saprophytic fungi colonizing wheat kernels to fungicides.
Moreover, the quantification of specific mRNA transcription by real-time PCR is being increasingly applied to the study of changes in gene expression in response to phytopathogenic and antagonistic fungi.
Spraying control wlth scraping for cytospora canker of poplar and selection trials in field have been carried by culturing five antagonistic fungi species selected before on solid medium (PDA) and Czapek' s solution and obtaining spores and fermentation solution of these species, Trichoderma sp. , penicillium sp., Aspergillus sp. , Eurotium so. and Fusarium sp. The results tested in lab. show that: The inhibition effects of Trickoderma sp. and Fusarium sp. are better than that of others by culture method...
Spraying control wlth scraping for cytospora canker of poplar and selection trials in field have been carried by culturing five antagonistic fungi species selected before on solid medium (PDA) and Czapek' s solution and obtaining spores and fermentation solution of these species, Trichoderma sp. , penicillium sp., Aspergillus sp. , Eurotium so. and Fusarium sp. The results tested in lab. show that: The inhibition effects of Trickoderma sp. and Fusarium sp. are better than that of others by culture method of replica-plating; All culture filtrates of these species show obvious inhibition effects on the germination of pathsgenic spores; In the inoculation test, the effect of antagonists inoculated first is the best (33.3%-100%) , the mixed inoculation is second (0.0%-72.2%) and afterwards inoculated is the worst (5.6%-33.3%). The results of selection test in field show that : The control effects of spore suspension solution is better than that of fermentation solution, and the Trickoderma sp. and the Fusarium sp. are the best as well as surviving longer on the trunk among them. The cured rate is above 7.0% but for Aspergillus sp..
193 strains of bacteria and 90 strains of fungi were isolated and purified from crop stalks and cotton soil samples. Among them 26 strains of antagonistic bacteria and antagonistic fungi to Phytophthora boehmeriae were selected by using pair culture method. The bioassay indicated that the treatment of inoculating sporangia of P.boehmeriae on cotton bolls 24 h after applying the antagonistic bacteria (Bacillus sp. MB 3, MB 7, MB 8, BC 1) was much better than the treatment of inoculating...
193 strains of bacteria and 90 strains of fungi were isolated and purified from crop stalks and cotton soil samples. Among them 26 strains of antagonistic bacteria and antagonistic fungi to Phytophthora boehmeriae were selected by using pair culture method. The bioassay indicated that the treatment of inoculating sporangia of P.boehmeriae on cotton bolls 24 h after applying the antagonistic bacteria (Bacillus sp. MB 3, MB 7, MB 8, BC 1) was much better than the treatment of inoculating the sporangia immediately after applying the bacteria. The control effects were 60%-80% and 30%-40% respectively. The relative control effects against cotton boll phytophthora blight by MB 3 and MB 7 were 34.9% and 45.4% in the field testing, and were significantly higher than that in the chemical control. In addition, the action mechanism of antagonists were also briefly discussed.
When four antagonistic fungi (Trichoderma koningii, T. pseudokoningii, T. harzianum and Gliocladium spp. ) were irradiated with ultraviolet light for 30 min, mutants of strains were obtained. Mutants did not change the morphological characters and antagonistic activity to Fuserium axysporum f. sp. melonis and Sclerotinia minor when compared with the wild type. Resistance to iprodione and copper oxychloride was found to occur in all mutants. Some of them, such as mutants of T. Koningii, T. harzianum...
When four antagonistic fungi (Trichoderma koningii, T. pseudokoningii, T. harzianum and Gliocladium spp. ) were irradiated with ultraviolet light for 30 min, mutants of strains were obtained. Mutants did not change the morphological characters and antagonistic activity to Fuserium axysporum f. sp. melonis and Sclerotinia minor when compared with the wild type. Resistance to iprodione and copper oxychloride was found to occur in all mutants. Some of them, such as mutants of T. Koningii, T. harzianum and Gliocladium spp, resisted to carbendazim. None of the mutants resisted to propiconazole.
antagonistic fungi
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