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胞浆内精子注射
相关语句
  intracytoplasmic sperm injection
     Normal Mice Derived from Oocytes Following Intracytoplasmic Sperm Injection (ICS I)
     胞浆内精子注射技术生产小鼠
短句来源
     Advance of study on intracytoplasmic sperm injection in mammals
     卵母细胞胞浆内精子注射(ICSI)技术的研究进展
短句来源
     Studies on the Intracytoplasmic Sperm Injection in the Mouse
     小鼠胞浆内精子注射的研究
短句来源
     In the present study, intracytoplasmic sperm injection (ICS1) was successful in the mouse when the piezo-driven micropipette was used and the possibility of making transgenic mouse by the ICSI method was tested.
     本研究以piezo操作系统为技术支撑,在掌握小鼠卵母细胞胞浆内精子注射技术(ICSI)的基础上,进行了ICSI技术生产试管小鼠及转基因小鼠的尝试。
短句来源
     This paper describes the use of piezo-driven micropipe tte for intracytoplasmic sperm injection of mice eggs.
     以piezo操作系统为技术支撑 ,在掌握小鼠卵母细胞胞浆内精子注射技术 (ICSI)的基础上 ,进行了ICSI技术生产试管小鼠的尝试。
短句来源
  “胞浆内精子注射”译为未确定词的双语例句
     Compaison of in - vitro development of embryos originating either conventional in - vitro fertilization or intra-cytoplasmic sperm injection
     卵细胞与胞浆内精子注射胚胎体外发育情况分析
短句来源
     Objective: To compare in - vitro development of embryos originating from conventiond in- vitro fertilization (IVF) and intra-cytoplasmic sperm injection.
     目的:比较传统的体外受精(IVF)与胞浆内精子注射(ICSI)胚胎体外发育情况。
短句来源
  相似匹配句对
     Studies on the Intracytoplasmic Sperm Injection in the Mouse
     小鼠胞浆精子注射的研究
短句来源
     Normal Mice Derived from Oocytes Following Intracytoplasmic Sperm Injection (ICS I)
     胞浆精子注射技术生产小鼠
短句来源
     Clinical study of intracytoplasmic sperm injection(ICSI)
     卵母细胞胞浆精子注射的临床应用研究
短句来源
     Research Progresses in Bovine Intracytopiasmic Sperm Injection
     牛卵母细胞胞浆精子注射的研究进展
短句来源
     Advance of study on intracytoplasmic sperm injection in mammals
     卵母细胞胞浆精子注射(ICSI)技术的研究进展
短句来源
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  intracytoplasmic sperm injection
Recent success in assisted fertilization mainly depended on the development of sperm microinjection methods: intracytoplasmic sperm injection and subzonal insemination.
      
Some basic mechanisms that under-lie fertilization were revealed by using intracytoplasmic sperm injection.
      
In respect to this, problems of fertility, oocyte activation, formation of pronuclei and practical aspects of intracytoplasmic sperm injection are discussed.
      
Cytogenetic analysis of human spermatozoa using intracytoplasmic sperm injection into mouse oocytes
      
With the advent of assisted reproductive technology, testicular biopsy is now used therapeutically to retrieve sperm for intracytoplasmic sperm injection (ICSI).
      
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During the past 5~10 years, very few medical fields have changed dramatically as reproductive medicine, especially for the new treatment for the male infertility by the use of intracytoplasmic sperm injection (ICSI) techniques and advanced surgical epididymal and testicular sperm retrieval techniques. Those two major technical advances have completely changed the treatment for previously untreatable testicular failure (non obstructive azoospermia), ejaculatory failure or un reconstructable obstructive azoospermia....

During the past 5~10 years, very few medical fields have changed dramatically as reproductive medicine, especially for the new treatment for the male infertility by the use of intracytoplasmic sperm injection (ICSI) techniques and advanced surgical epididymal and testicular sperm retrieval techniques. Those two major technical advances have completely changed the treatment for previously untreatable testicular failure (non obstructive azoospermia), ejaculatory failure or un reconstructable obstructive azoospermia. Many men with even the most severe forms of infertility that are not amendable to specific medical and surgical treatments can now have chances to father biological children with only a few of their spermatozoa. This review serves to discuss the various advanced sperm retrieval techniques available for assisted reproduction, with specific focus on the technical aspects and safety. Each technique has its specific medical indications, efficacies and limitations.

在过去的 5~ 10年里 ,医学领域中很少有像生殖医学那样发展迅速 ,特别是使用卵胞浆内精子注射 (ICSI)技术治疗男性不育及先进的外科附睾和睾丸取精术。这两项重大技术进步彻底改变了原先不可治愈的睾丸衰竭 (非梗阻性无精子症 )、射精障碍或不可修复的梗阻性无精子症。那些患有非常严重的不能用特殊方法和手术治疗的不育男性 ,现在有机会利用他们仅有的几条精子做起其生物学子女的父亲。讨论了辅助生育的各种先进的精子取出术 ,重点讨论技术和安全性。每一技术有其特殊的医学适应证、功效和局限性

Objective: Using rabbit as a model to evaluate the feasibility of the germinal vesicle (GV)transfer. Methods: Collecting the GV stage oocytes from the ovaries of white Japan big ear rabbits at 72 h after the first follicle stimulating hormone injection. The GV-karyoplast was exchanged between the primary oocytes, the pairs of GV karyoplast-cytoplast were fused by electrical pulses, and then the reconstructed oocytes were maturated in TCM-199 with 10% FCS. Finally, the matured reconstructed oocytes were fertilized...

Objective: Using rabbit as a model to evaluate the feasibility of the germinal vesicle (GV)transfer. Methods: Collecting the GV stage oocytes from the ovaries of white Japan big ear rabbits at 72 h after the first follicle stimulating hormone injection. The GV-karyoplast was exchanged between the primary oocytes, the pairs of GV karyoplast-cytoplast were fused by electrical pulses, and then the reconstructed oocytes were maturated in TCM-199 with 10% FCS. Finally, the matured reconstructed oocytes were fertilized by intracytoplasmic sperm injection (ICSI) with the same species' sperms. Results: A total of 987 GV stage oocytes were manipulated. 272 (27.6%) GV-karyoplasts were removed and replaced successfully without lysis. Karyoplast fusion was accomplished in 230 for a final success rate of 84.6% (230/272). After in vitro maturation 178 of 230 (77.4%) reached metaphase II (emitted first polar body). The fertilization rate of matured reconstructed oocytes by ICSI was 70.8% (126/178). Conclusion: The result indicated that GV transfer was feasible, which could potentially become a new approach in treatment of women infertility due to advanced maternal age.

目的 :以家兔为实验动物模型 ,探索卵母细胞生发泡 (GV)互换的可行性。方法 :在注射第一针卵泡刺激素 (FSH) 72 h后 ,取大耳白兔卵巢内 GV期卵母细胞 ,用显微操作和电融合的方法 ,对兔初级卵母细胞进行异卵间 GV互换 ,将重构卵培养于 TCM-1 99加 1 0 % FCS中 ,对重构卵进行成熟培养 ,最后对成熟重构卵进行卵胞浆内精子注射(ICSI)和胚胎培养。结果 :对 987枚 GV期卵母细胞进行了异卵间 GV互换 ,成功互换 2 72枚 (2 7.6 % ) ,电融合后存活 2 30枚 (84.6 % ) ,在进行成熟培养后 ,2 30枚卵有 1 78枚(77.4% )发育到 MII期 (排出第一极体 ) ,将成熟卵进行 ICSI后 ,有 1 2 6枚 (70 .8% )受精。结论 :对兔初级卵母细胞进行 GV互换是可行的 ,若将此法应用于提高高龄妇女卵母细胞的质量 ,将有助于解决她们的不育问题。

Objective: To compare in - vitro development of embryos originating from conventiond in- vitro fertilization (IVF) and intra-cytoplasmic sperm injection. Methods: 1. Retrieved sibling oocytes from one patient were randomly assigned to conventional IVF or ICSI. To compare fertilization rate and formation of good - quality embryo between two groups. 2. Retrospectively analysis in - vitro development of embryos in 154 conventional IVF patients and 34 ICSI patients, comparing fertilization rate and good - quality...

Objective: To compare in - vitro development of embryos originating from conventiond in- vitro fertilization (IVF) and intra-cytoplasmic sperm injection. Methods: 1. Retrieved sibling oocytes from one patient were randomly assigned to conventional IVF or ICSI. To compare fertilization rate and formation of good - quality embryo between two groups. 2. Retrospectively analysis in - vitro development of embryos in 154 conventional IVF patients and 34 ICSI patients, comparing fertilization rate and good - quality embryo formation between two groups. Results: 1 :The fertilization rate in IVF group was not lower than that in ICSI group(63.0% ,87/138 VS 65.2% ,86/132,P > 0.05}but it was lower than 84.3 % (86/102) which was the fertilization rate in ICSI group of per injected oocyte, There were not significant difference of good - quality embryo formation between two groups (54.0 % vs 55. 8 % , P > 0.05). 2. Retrospectively analysis reveals that total fertilization failure in IVF group was 7.9% (12/154) but not seen in ICSI group.There are not significant difference of fertilization rate between two groups (61.5% VS 60.1%P> 0.05) ,but significant difference was seen when based on the number of MII oocytes injected with sperm(61.5% VS 77.6 % , P < 0.05). There were not significant difference of good - quality embryo formation between two groups (52.9 % VS 56. 1 % , P > 0. 05) . Conclusion: Intracytoplasmic sperm injection can increase the fertilization rate but can not decrease good - quality embryo formation. If conventional IVF and ICSI were used in patient s sibling oocytes, the problem of total fertilization failure can be avoided.

目的:比较传统的体外受精(IVF)与胞浆内精子注射(ICSI)胚胎体外发育情况。方法:1.将同一病人卵子随机平均分为两部分,分别实施IVF与ICSI技术,比较两组受精率与优质胚胎率。2.回顾性分析154例IVF与34例ICSI胚胎体外发育情况,比较两组受精率与优质胚胎率。结果:1.分组比较结果显示,IVF组与ICSI组受精率相比,以总卵子数计算未见显著性差异[63.0%(87/138)与65.2%(86/132);P>005];但当ICSI组以注射卵子数计算时则两者间有显著性差异[63.0%(87/138)与84.3%(86/102);P<0.05]。优质胚胎率两组间未见显著性差异[54.0%(47/87)与55.8%(48/86);P>0.05]。2.回顾性分析结果显示,IVF组未受精率7.9%(12/154),而ICSI组未见此现象发生。IVF组ICSI组受精率相比,以总卵子数计算未见显著性差异(61.5%比60.1%,P>0.05);当ICSI组以注射卵子数计算时则有显著性差异(61.5%比77.6%,P<0.05)。优质胚胎率两组间未见显著性差异(52.9%比56.1%,P>0.05)。结...

目的:比较传统的体外受精(IVF)与胞浆内精子注射(ICSI)胚胎体外发育情况。方法:1.将同一病人卵子随机平均分为两部分,分别实施IVF与ICSI技术,比较两组受精率与优质胚胎率。2.回顾性分析154例IVF与34例ICSI胚胎体外发育情况,比较两组受精率与优质胚胎率。结果:1.分组比较结果显示,IVF组与ICSI组受精率相比,以总卵子数计算未见显著性差异[63.0%(87/138)与65.2%(86/132);P>005];但当ICSI组以注射卵子数计算时则两者间有显著性差异[63.0%(87/138)与84.3%(86/102);P<0.05]。优质胚胎率两组间未见显著性差异[54.0%(47/87)与55.8%(48/86);P>0.05]。2.回顾性分析结果显示,IVF组未受精率7.9%(12/154),而ICSI组未见此现象发生。IVF组ICSI组受精率相比,以总卵子数计算未见显著性差异(61.5%比60.1%,P>0.05);当ICSI组以注射卵子数计算时则有显著性差异(61.5%比77.6%,P<0.05)。优质胚胎率两组间未见显著性差异(52.9%比56.1%,P>0.05)。结论:应用胞浆内精子注射有较高的受精率,且不会降低优质胚胎率。将回收卵子同时行IVF与ICSI能避免受精失败的发生。

 
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