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design with plants
相关语句
  相似匹配句对
     In design, .
     设计过程中应用了.
短句来源
     THE DESIGN ON DOWTHERM IN POLYAMIDE PLANTS
     聚酰胺装置的导生系统设计
短句来源
     Its design
     在Matlab环境下,采用复合形法,编写了优化设计的程序框图。
短句来源
     The Functions Of Plants In The Landscape Design
     植物在园林造景中的作用
短句来源
     Phosphoglucomutase in Plants
     植物中的葡糖磷酸变位酶
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In order to providing a reasonable foundation for landscape design with plant,an application of SBE(Sc enic Beauty Estimation Proce-dures)based on a survey and the photographs taken from the vegetation landscape in Guangzhou parks to ac-quiring the best plant d isposition which embodying the characteristics of landscape architecture of Ling nan style and the south subtropical distin-guishing features,was described in t his paper.

通过对广州市公园现有植物景观的调查,摄取相关景观的照片,采用风景质量评价方法,寻求在城市公园中为公众所喜爱,既能体现岭南园林风格,又具有南亚热带植物景观特色的最佳植物配置模式,为植物造景提供科学的依据。

According to the amino acid sequence of Vitreoscilla hemoglobin (VHb), a novel gene encoding the VHb protein was designed with plant preferential codon. First 22 oligodeoxynucleotide fragments were synthesized based on the design, meanwhile, certain restriction enzyme sites for further cloning were considered. Three small DNA fragments, which were formed by annealing certain oligonucleotide fragments, were sub-cloned respectively. Then the vgbM gene, which is 450 bp in length, was cloned into...

According to the amino acid sequence of Vitreoscilla hemoglobin (VHb), a novel gene encoding the VHb protein was designed with plant preferential codon. First 22 oligodeoxynucleotide fragments were synthesized based on the design, meanwhile, certain restriction enzyme sites for further cloning were considered. Three small DNA fragments, which were formed by annealing certain oligonucleotide fragments, were sub-cloned respectively. Then the vgbM gene, which is 450 bp in length, was cloned into pUC19 by ligating the three small DNA fragments. The recombinant plasmid was named pGSVHB. The pBV221SVHB was further constructed by inserting the vgbM gene into the plasmid pBV221 after a Nco I site adding at 5’-terminal through PCR. Subsequently, the vector was introduced into E. coli, BL21 strain. The 16 kD VHb protein was detected after hot-shock inducing expression of the engineered E. coli. The biological activity of the expressed VHb was demonstrated by CO difference spectrum analysis. This work has laid a foundation for further studies to transfer vgbM gene into plants.

根据透明颤菌血红蛋白(VHb)的氨基酸序列,按照植物偏爱密码子,对透明颤菌血红蛋白基因(vgb)进行优化改造,同时考虑到可实现分别克隆拼接的酶切位点,设计并合成了22条单链DNA短片段,通过拼接获得了全长450bp的透明颤菌血红蛋白基因vgbM,并将其克隆至pUC19上,获得重组质粒pGSVHB。利用设计的引物,通过PCR在vgbM基因的5′端引入NcoⅠ位点,将其克隆到原核生物高效表达载体pBV221上,获得质粒pBV221SVHB。转化E.coli.BL21并经热激诱导表达后,在培养物中检测到了约16kD的VHb蛋白。经CO差示光谱分析测定,该蛋白具有生物活性。为透明颤菌血红蛋白基因vgbM在植物基因工程研究中的应用奠定了基础。

 
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