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摇瓶     
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  shake flask
    Fermentation process and engineering parameters obtained from bacillus thuingiensis shake flask
    苏云金杆菌摇瓶发酵工艺与工程参数
短句来源
    By the fermentation in shake flask,it was studied that the influence of temperature,the shaking speed of shake flask,the kind and concentration of carbon source and the kind and concentration of nitrogenous source on the production of dicarboxylic acid by fermentation of Candida tropicalis.
    通过摇瓶发酵,研究温度、摇瓶转速、碳源种类和浓度、氮源种类和浓度对热带假丝酵母(Candida tropicalis)产酸的影响.
短句来源
    Under the optimal fermentation conditions,the activity of fibrinolysin reached 3643 U/ml(urokinase unit) in shake flask,and 2050 U/ml(urokinase unit) in 15 L fermentor.
    在最适发酵条件下,摇瓶发酵产豆豉溶栓酶酶活达到3643U/mL发酵液(尿激酶单位),15L发酵罐发酵产豆豉溶栓酶酶活达到2050U/mL发酵液(尿激酶单位)。
短句来源
    The changes of process parameters between the shake flask and the 7 L stirred bioreactor were quite different. The maximum EPS production and productivity were 3.283 /L and 0.027 g/L h in the fermentor,increased by 26.5% and 50% compared with that in flask.
    经7 L发酵罐放大实验表明,桑黄代谢与摇瓶存在一定差异,胞外多糖的合成速度更快,于120 h达最大值3.283 g/L,生产强度为0.027 g/(L.h),与摇瓶相比分别提高了26.5%和50%。
短句来源
    Studies on L Lysine Producing Strain FH128 (Ⅰ) The Screening of L Lysine Producing Strain FH128 and Shake Flask Culture
    L-赖氨酸产生菌FH128菌株的研究(Ⅰ)FH128菌株的选育及摇瓶发酵
短句来源
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  shaking flask
    Compared with shaking flask experiment,the MBR system effectively prevented the product inhibition of the enzyme and vanillin oxidation loss,and the bioconversion rate of clove oil increased to 1.04% from 0.45%. The maximum vanillin concentration in receiving solution was 130.10 mg/L which was much higher than 11.13 mg/L in the shaking flask experiment.
    与摇瓶实验相比,在很大程度上消除了产物的抑制和香草醛的氧化损失,丁香油转化率从0.45%提高到1.04%,香草醛在下游萃取液中的最高质量浓度达到了130.10 mg/L,远高于摇瓶实验的11.13 mg/L;
短句来源
    Influences of carbon and nitrogen sources on Pseudomonas putida S1 dry weight and on the activity of intracellular trehalose synthase,were studied in shaking flask scaled Fermentation.
    采用摇瓶发酵法研究了不同碳源、氮源对恶臭假单胞菌S1干重和胞内海藻糖合成酶酶活力的影响。
短句来源
    Under the condition of shaking flask fermentation,by means of orthogonal test,the effects of nutrition factors,such as glucose,soybean powder,glycerol and 95% ethanol,on the production of phenazine-1-carboxylic acid(PCA) by a genetic engineering strain M18G. The optimized components of culture medium were 6g glucose,22g soybean powder,6mL glycerol,5.9mL 95% ethanol in 1 liter;
    本试验针对高产PCA的基因工程菌M18G,首先在摇瓶培养条件下,用正交试验法研究了培养基中各主要营养因子葡萄糖、黄豆粉、甘油、95%乙醇等对PCA产量的影响,并确定了适合PCA分泌的最佳培养基(1L)为:葡萄糖6g,黄豆粉40g,甘油6mL,95%乙醇5.9mL;
短句来源
    Transglutaminase (MTG) Fermentation with Streptomyces hygroscopicus in Shaking Flask
    Streptomyces hygroscopicus产谷氨酰胺转胺酶摇瓶发酵条件的优化
短句来源
    With t-PA activity as target ,the high-producing Trichoderma reesei (8) with highly genetic stability was selected by shaking flask liquid cultivation of the strains from single colony on plate.
    采用稀释平板分离法对基因工程菌株Trichoderma reesei进行了分离纯化,对平板上生长良好的单菌落进行摇瓶液态发酵,以t-PA的酶活力为指标,挑选高产菌株,选得的Trichoderma reesei(8)号菌株t-PA产量最高,且遗传稳定性良好。
短句来源
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  shake-flask
    The yield of NAF-032 in the shake-flask could be up to 123.3 g/L in the optimal fermentation condition and medium.
    在最适发酵条件下,米根霉NAF-032的摇瓶发酵产L-乳酸产量达123.3 g/L.
短句来源
    The Study on the Screening for Hyaluronic Acid Producer Streptococcus zooepidemicuc NUF-035 and Its Fermentation Technologies in Shake-Flask Culture
    生产透明质酸的兽疫链球菌NUF-035的筛选及摇瓶发酵条件
短句来源
    OPTIMIZATION CONDITIONS ON FERMENTATION PROCESS OF L-LEUCINE PRODUCING STRAIN TK0303 IN SHAKE-FLASK
    L-亮氨酸产生菌TK0303的摇瓶发酵条件优化
短句来源
    3) The cultivation conditions of strain E. coli BL21 (DE3) (pET28-A.R-hemA) was evaluated at shake-flask scale.
    3) 研究了重组菌E coli BL21(DE3)(pET28-A.R-hemA)的摇瓶发酵条件。
短句来源
    The optimal medium composition in shake-flask scale were determined as followings: sucrose 59.80 g/L; tryptone 53.54 g/L; L-glutamine 81.05g/L;
    通过对摇瓶的培养条件优化,得到了如下优化的培养基成分为:蔗糖59.80g/L,蛋白胨53.54g/L,,L-谷氨酸81.05g/L,NaCl 10g/L,MgSO_4·7H_2O 1.0g/L,CaCl_2 1.0g/L;
短句来源
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  shake flasks
    Inoculation volume is 5%. Culture was carried out in Shake Flasks(250ml)containing 50ml medium,all flasks were incubated for five days in an shaker(120rpm,30℃),and the xylanase activity of culture filtrate came up to 7.74IU/mL.
    在装料50mL的三角摇瓶中于30℃、120 rpm振荡培养5d后,发酵液中木聚糖酶活力达7.74 IU/mL。
短句来源
    Optimization of Acinetobater sp. Culture Conditions in Shake Flasks for Efficient Biosynthesis of a Novel Carboxylesterase
    不动杆菌生物合成新型羧酸酯酶的摇瓶培养条件优化
短句来源
    The ratio of C to N affected the secretion of extracellular inulinase. The cells growth conditions of K. fragilis were initial pH 5.5 ~ 6.0, 28 ℃ about 26 ~ 28 hours in shake flasks ( 200rpm ) in the medium YID.
    脆壁克鲁维氏酵母发酵生产菊糖酶过程中,从斜面接到种子培养基YID中,培养基初始pH为5.5~6.0,在28℃下摇瓶振荡培养(200rpm)26~28小时,当培养液中OD_(550)×10~(-1)约为0.4时,作为种子液接入发酵培养基中进行发酵生产菊糖酶。
短句来源
    The maximal biomass concentration (dry weight) was 19.8g/L in shake flasks, and cell lutein content reached 2.63mg/g.
    其摇瓶培养的最高生物量(干重)为19.8g/L,最大比生长速率为0.76d~(-1),干细胞中叶黄素含量为2.63mg/L。
短句来源
    When the growth of sb-02 in shake flasks at 28 C for 96hours with initial pH6.0 and 2% bran on hemicellulose substrates it showed high activity of xylanase.
    结果表明,初始pH6.0,温度28℃,麸皮添加量为2%,摇瓶培养至96h时酶活力达到高峰。
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  shake flask
An endophyte (designated ZP5SE) was isolated from the seed of Nothapodytes foetida and was examined as a potential source of anticancer drug lead compound, i.e., camptothecin, when grown in Sabouraud liquid culture media under shake flask conditions.
      
ZD-1 were investigated in hydrocarbon aqueous biphasic (O/W) systems in shake flask.
      
A mixed culture of moderately thermophilie microorganisms was enriched from acid mine drainages(AMDs) samples collected from several sulphide mines in China, and the bioleaching of chalcopyrite was conducted both in shake flask and bioreactor.
      
The results show that in the shake flask, the mixture can tolerate 50 g/L chalcopyrite after being acclimated to gradually increased concentrations of chalcopyrite.
      
In this work, we also developed a simple technique forin situ measuring oxygen uptake rate (OUR) and carbon dioxide evolution rate (CER) in SSF in a shake flask based on the principle of Warburg manometer.
      
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  shaking flask
Several sets of shaking flask experiments were conducted.
      
Sequential methodology based on the application of three types of experimental designs was used to optimize the fermentation conditions for elastase production from mutant strain ZJUEL31410 of Bacillus licheniformis in shaking flask cultures.
      
To optimize the fed-batch processes of glycerol fermentation in different reactor states, typical bioreactors including 500-mL shaking flask, 600-mL and 15-L airlift loop reactor, and 5-L stirred vessel were investigated.
      
Scale-up of purine nucleoside fermentation from a shaking flask to a stirred-tank fermentor
      
Scaling-up purine nucleoside fermentation by a mutant strain of Bacillus subtilis from a shaking flask to a stirred-tank fermentor was attempted.
      
更多          
  shake-flask
Pleurotus ostreatus mushroom mycelium was cultivated in submerged culture in shake-flask experiments with acid extract from peat and yeast extract as nutrient sources.
      
Enzyme activities produced by the immobilized cell system in a bubble column were higher than that of shake-flask culture.
      
Poor correlation was found between in vivo N-methyltransferase activity of cyclosporin A synthetase of the mutant grown on agar plate and the actual amount of cyclosporin A production in shake-flask culture.
      
However, when the cells grown on the shake-flask culture were applied in the in vivo [14C-methyl] labeling assay, a better correlation was resulted.
      
From the shake-flask experiments, the optimal medium composition was defined for improved lactic-acid production.
      
更多          
  shake flasks
The addition of Ag2S selectively increased the copper dissolution from the chalcopyrite-containing ores in shake flasks with a recovery of 85.3% compared with 24.3% without Ag2S, while slightly decreased the iron yields from 51% to 41.8%.
      
An initial pH of 5.0 was found to be optimal for the production of cellulase in shake flasks; this was attained in about 6-8 d in a medium containing either cellulose or rice straw as the sole source of carbon.
      
The production of pigment-free pullulan byAureobasidium pullulans, using olive oil and sucrose as carbon (C) sources, in shake flasks, was investigated.
      
Cellulase production using various biomass substrates available for SHF, including paper sludge, pretreated wood (steam exploded), and their hydrolysis residues, was investigated in shake flasks and a fermenter for their productivities and titers.
      
Xylanase production of Trichoderma reesei Rut C-30 was examined at different initial pH values (4.8, 5.9, and 7.0) on rice straw in shake flasks, and in a fermentor, for the best pH condition.
      
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