By the fermentation in shake flask,it was studied that the influence of temperature,the shaking speed of shake flask,the kind and concentration of carbon source and the kind and concentration of nitrogenous source on the production of dicarboxylic acid by fermentation of Candida tropicalis.
The changes of process parameters between the shake flask and the 7 L stirred bioreactor were quite different. The maximum EPS production and productivity were 3.283 /L and 0.027 g/L h in the fermentor,increased by 26.5% and 50% compared with that in flask.
Compared with shaking flask experiment,the MBR system effectively prevented the product inhibition of the enzyme and vanillin oxidation loss,and the bioconversion rate of clove oil increased to 1.04% from 0.45%. The maximum vanillin concentration in receiving solution was 130.10 mg/L which was much higher than 11.13 mg/L in the shaking flask experiment.
Under the condition of shaking flask fermentation,by means of orthogonal test,the effects of nutrition factors,such as glucose,soybean powder,glycerol and 95% ethanol,on the production of phenazine-1-carboxylic acid(PCA) by a genetic engineering strain M18G. The optimized components of culture medium were 6g glucose,22g soybean powder,6mL glycerol,5.9mL 95% ethanol in 1 liter;
Inoculation volume is 5%. Culture was carried out in Shake Flasks(250ml)containing 50ml medium,all flasks were incubated for five days in an shaker(120rpm,30℃),and the xylanase activity of culture filtrate came up to 7.74IU/mL.
The ratio of C to N affected the secretion of extracellular inulinase. The cells growth conditions of K. fragilis were initial pH 5.5 ~ 6.0, 28 ℃ about 26 ~ 28 hours in shake flasks ( 200rpm ) in the medium YID.
An endophyte (designated ZP5SE) was isolated from the seed of Nothapodytes foetida and was examined as a potential source of anticancer drug lead compound, i.e., camptothecin, when grown in Sabouraud liquid culture media under shake flask conditions.
ZD-1 were investigated in hydrocarbon aqueous biphasic (O/W) systems in shake flask.
A mixed culture of moderately thermophilie microorganisms was enriched from acid mine drainages(AMDs) samples collected from several sulphide mines in China, and the bioleaching of chalcopyrite was conducted both in shake flask and bioreactor.
The results show that in the shake flask, the mixture can tolerate 50 g/L chalcopyrite after being acclimated to gradually increased concentrations of chalcopyrite.
In this work, we also developed a simple technique forin situ measuring oxygen uptake rate (OUR) and carbon dioxide evolution rate (CER) in SSF in a shake flask based on the principle of Warburg manometer.
Several sets of shaking flask experiments were conducted.
Sequential methodology based on the application of three types of experimental designs was used to optimize the fermentation conditions for elastase production from mutant strain ZJUEL31410 of Bacillus licheniformis in shaking flask cultures.
To optimize the fed-batch processes of glycerol fermentation in different reactor states, typical bioreactors including 500-mL shaking flask, 600-mL and 15-L airlift loop reactor, and 5-L stirred vessel were investigated.
Scale-up of purine nucleoside fermentation from a shaking flask to a stirred-tank fermentor
Scaling-up purine nucleoside fermentation by a mutant strain of Bacillus subtilis from a shaking flask to a stirred-tank fermentor was attempted.
Pleurotus ostreatus mushroom mycelium was cultivated in submerged culture in shake-flask experiments with acid extract from peat and yeast extract as nutrient sources.
Enzyme activities produced by the immobilized cell system in a bubble column were higher than that of shake-flask culture.
Poor correlation was found between in vivo N-methyltransferase activity of cyclosporin A synthetase of the mutant grown on agar plate and the actual amount of cyclosporin A production in shake-flask culture.
However, when the cells grown on the shake-flask culture were applied in the in vivo [14C-methyl] labeling assay, a better correlation was resulted.
From the shake-flask experiments, the optimal medium composition was defined for improved lactic-acid production.
The addition of Ag2S selectively increased the copper dissolution from the chalcopyrite-containing ores in shake flasks with a recovery of 85.3% compared with 24.3% without Ag2S, while slightly decreased the iron yields from 51% to 41.8%.
An initial pH of 5.0 was found to be optimal for the production of cellulase in shake flasks; this was attained in about 6-8 d in a medium containing either cellulose or rice straw as the sole source of carbon.
The production of pigment-free pullulan byAureobasidium pullulans, using olive oil and sucrose as carbon (C) sources, in shake flasks, was investigated.
Cellulase production using various biomass substrates available for SHF, including paper sludge, pretreated wood (steam exploded), and their hydrolysis residues, was investigated in shake flasks and a fermenter for their productivities and titers.
Xylanase production of Trichoderma reesei Rut C-30 was examined at different initial pH values (4.8, 5.9, and 7.0) on rice straw in shake flasks, and in a fermentor, for the best pH condition.