We have used the two-step protocol PCR to detectDNA sequence related to HPV16 E_6/E_7 in 34 cervical cancer tissues, by primingwith SP and ASP, the positive percentage of HPV 16 E_6/E_7 in cervical cancerswas 67.6% (23/34).
Results The quantities of HPV 16 E6 gene were 0.3±0.4, 0.6±0.4, 1.8±0.6 and 2.4±0.6, and expression rates of survivin were 7%, 31%, 63% and 84% respectively in chronic cervicitis, CINⅠ、CINⅡ-Ⅲ and cervical cancer tissues.
3.The expression rate of PTTG protein in cervical cancer tissues with the HPV16/18E6 protein coexpression was higher than that in those without HPV16/18E6 protein coexpression (P<0.05).
RESULTS: (1) The 5′-CpG islands methylation rate of FHIT gene in cervical cancer tissues was 40.0% (16/40), while no methylation of FHIT gene was found in normal cervical tissues.
Objective: To investigate the expression and clinicopathological significance of HER-2/neu(c-erbB-2)and Ras oncogene in cervical cancer tissue and cell lines,and to evaluate the effect and correlation of HER-2/neu and Ras in cervix cancer.
Methods:Semi-quantitative RT-PCR was used to investigate the expression of RPL39L mRNA in CIN tissue,cervical cancer tissue and adjacent normal tissue.
Results The positive rates of MMP2, MMP9 expression in cervical carcinoma tissue were 27.9%, 25.6%, which were significantly higher than those in the corresponding normal tissues (P<0.05 and 0.01).
The aim of the present study was to investigate the genetic instability in cervical carcinoma tissues and provide evidence for discovering new tumor suppressor genes and screening diagnostic molecular marker of cervical carcinoma.
A common sequence variation of human papillomavirus Type 16 in cervical cancer cell lines and in cervical cancer tissues from Korean patients was recently reported.
This variant has been detected in cervical cancers from Korean patients: 19 (39 percent) of 49 cervical cancer tissues and 6 (50 percent) of 12 cervical cancer cell lines.
Although E5 was expressed in all of the HSIL and cervical cancer tissues, but in only one of the eight normal tissues tested, only WT E5 protein was found in HSIL while in cervical cancer tissues both WT and mutant E5 proteins were detected.
The results showed also that the Fe level and Cu/Zn ratio were significantly higher and the Zn and Se levels were significantly lower in cervical cancer tissue than in uterine myoma tissue (p>amp;lt;0.01 and p>amp;lt;0.001, respectively).