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biosensor technology
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  生物传感器
     Recent Developments in SPR Biosensor Technology:Application of BIACORE J
     表面等离子体共振(SPR)生物传感器技术的进展——BIACORE J 的应用
短句来源
     It has been applied to isolate, quantify protein and analyse protein-protein, protein-small molecule interaction. Protein chip combinated with mass spectrometry, biosensor technology expended its applicable scale. It also introduced the advance of proteome chip and alive chip in proteomics research.
     本文综述了蛋白质芯片在蛋白质组学研究中的多种作用,包括普通蛋白质芯片在微量蛋白质分离、蛋白质与蛋白质之间以及蛋白质与其他小分子间相互作用和蛋白质定量检测方面的作用,普通蛋白质芯片通过与质谱技术、生物传感器技术的结合而拓展其应用范围,以及蛋白质组芯片、活性的蛋白质芯片在蛋白质组学研究中应用的进展。
短句来源
     Screening of anti-inflammatory Chinese traditional herbs targeted CpG ODN using biosensor technology
     以CpG ODN为靶点应用生物传感器技术筛选抗炎中药
短句来源
     SPR biosensor technology continues to evolve.
     SPR 生物传感器技术正在持续发展着。
短句来源
     In recent years,the use of AChE as biological recognition element in biosensor technology has gained more and more attention,in particular with respect to pesticides detection.
     近年来,AChE作为生物识别元素的生物传感器在杀虫剂残留量检测领域受到了广泛的关注。
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  生物传感器技术
     Recent Developments in SPR Biosensor Technology:Application of BIACORE J
     表面等离子体共振(SPR)生物传感器技术的进展——BIACORE J 的应用
短句来源
     It has been applied to isolate, quantify protein and analyse protein-protein, protein-small molecule interaction. Protein chip combinated with mass spectrometry, biosensor technology expended its applicable scale. It also introduced the advance of proteome chip and alive chip in proteomics research.
     本文综述了蛋白质芯片在蛋白质组学研究中的多种作用,包括普通蛋白质芯片在微量蛋白质分离、蛋白质与蛋白质之间以及蛋白质与其他小分子间相互作用和蛋白质定量检测方面的作用,普通蛋白质芯片通过与质谱技术、生物传感器技术的结合而拓展其应用范围,以及蛋白质组芯片、活性的蛋白质芯片在蛋白质组学研究中应用的进展。
短句来源
     Screening of anti-inflammatory Chinese traditional herbs targeted CpG ODN using biosensor technology
     以CpG ODN为靶点应用生物传感器技术筛选抗炎中药
短句来源
     SPR biosensor technology continues to evolve.
     SPR 生物传感器技术正在持续发展着。
短句来源
     Methods:(1) The biosensor technology was applied to screen the medicine-food plants, then isolate the active fractions from one of the medicine-food plants selected by the target of Lipid A.
     方法:(1)应用生物传感器技术,对大蒜、苦瓜、鱼腥草、生姜、芫荽等5种药食两用植物进行与Lipid A结合活性的筛选。
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  技术而
     BIA technology (biomolecular interaction analysis) is a new concept by using newly developed biosensor technology .
     BIA技术(biomolecular interaction analysis)——生物分子相互作用分析技术是利用生物传感技术而发展的全新概念。
短句来源
  “biosensor technology”译为未确定词的双语例句
     Results The neutralizing ratio of PMB to endotoxin as assessed by biosensor technology was 0.35 μg to 1 ng , while that by dynamic turbidimetric and chromogenic limulus amebocyte lysate(LAL) technique was 0.5mg to 1ng and 1mg to 1 ng, respectively. The results obtained by biotechnology were similar to that by biosensor technique.
     结果 用生物传感技术测定PMB中和内毒素能力的比值为 0 .35 μg∶1ng,动态浊度法为 0 .5mg∶1ng、基质显色法为 1.0mg∶1ng,生物学测定结果与生物传感技术测定结果相同。
短句来源
     Then aqueous extractions of the herbs with high affinity for CpG ODN were incubated with CpG ODN(33 μg·ml -1)at 37 ℃ for 30 min, subsequently the mixtures were tested using affinity biosensor technology.
     选择具有较高亲和力的中药水煎液,与CpGODN(33μg·ml-1)37℃孵育30min后,再次测定与CpGODN的亲和力。
短句来源
     Results:①The fraction 2 (DS2) with high binding activity was prepared by water extraction, polyamide gel chromatography, silica gel chromatography, ion exchange HPLC, affinity biosensor technology.
     结果:①利用水提取法、聚酰胺凝胶柱层析、硅胶柱层析、离子交换-HPLC等技术定向分离得到与lipid A有较高结合活性的组分:大蒜2号组分(DS2)。
短句来源
     Principle of surface plasmon resonance biosensor technology and its application in biomolecular interacting analysis
     表面等离子共振技术在生物分子互作研究中的应用
短句来源
     Study Advancement of a Surface Plasmon Resonance Biosensor Technology for Rapid Food Safety Testing
     表面等离子共振生物传感技术在食品安全快速检测中的应用研究进展
短句来源
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  biosensor technology
Available instruments utilizing biosensor technology are also examined from a commercial perspective.
      
Using biosensor technology, we found that PR3-ANCA of different patients (n = 8) recognize a limited number of overlapping regions on PR3 at the time of diagnosis of Wegener's granulomatosis.
      
Among these approaches, networks of excitable cells cultured on microelectrode arrays are uniquely poised to provide rapid, functional classification of an analyte and ultimately constitute a potentially effective cell-based biosensor technology.
      
This biosensor technology also holds potential for use in point-of-care diagnostics.
      
By using the disposable biosensor technology, this method is easy to follow, quick (within 2?min) and precise (with coefficients of variation to be as low as below 5%) and requires only a small amount of plasma sample (>amp;lt;50?μl).
      
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Radio labelled polynucleotide probes have been extensively employed for the detection of DNA hybridization. However, the drawbacks of the method are obvious: short lived of the probes, time consuming of operation, risk of exposure to radioactive. Recently considerable attention has been given to methods for incorporating non radioactivity labels into polynucleotides in order to circumvent the problems inherent in radioactivity. The method based on biosensor technology is attractive because it features...

Radio labelled polynucleotide probes have been extensively employed for the detection of DNA hybridization. However, the drawbacks of the method are obvious: short lived of the probes, time consuming of operation, risk of exposure to radioactive. Recently considerable attention has been given to methods for incorporating non radioactivity labels into polynucleotides in order to circumvent the problems inherent in radioactivity. The method based on biosensor technology is attractive because it features many advantages such as rapid, automatic and sensitive. The principles of the DNA sensors may be piezoelectric (PZ), surface plasma resonance (SPR), electrochemical, optical in combination of fluorescence, and so on. This paper summarizes the most of these sensors. Advantages and disadvantages of the sensor methods are discussed.

本文概述了当前生物传感器的研究特点以及发展DNA生物传感器的迫切性;从不同角度阐述了DNA生物传感器的概念和研究内容;着重讨论了DNA生物传感器的研究现状和发展趋势。文中分别对DNA光生物传感器和DNA压电晶体生物传感器的基本原理、特点、研究进展及存在的问题进行了分析与说明。进而,对我国DNA生物传感器研究存在的差距和发展前景进行了简要论述。

Protein protein interactions are implicated in every cellular processes such as DNA replication,transcription,translation,splicing,secretion,cell cycle control,signal transduction and intermediary metabolism.Some interactions in cells last long and others are transient.The specificities of these interactions are essential for the functions of proteins.Protein protein interactions can alter the kinetic properties of proteins,result in the formation of a new binding site,inactivate a protein,and change the specificity...

Protein protein interactions are implicated in every cellular processes such as DNA replication,transcription,translation,splicing,secretion,cell cycle control,signal transduction and intermediary metabolism.Some interactions in cells last long and others are transient.The specificities of these interactions are essential for the functions of proteins.Protein protein interactions can alter the kinetic properties of proteins,result in the formation of a new binding site,inactivate a protein,and change the specificity of a protein for its substrate.In the past few years,very rapid advances have been made in identification and characterization of protein protein interactions.Here,both classical and ,especially,recent methods to study protein protein interactions were summarized.First,biochemical methods such as protein affinity chromatography,affinity blotting,immunoprecipitation,cross linking were reviewed;Second,molecular biological methods such as protein probing,phage display,two hybrid system were described;Finally,a new biosensor technology based on surface plasmon resonance,which have become increasingly popular for the qualitative and quantitative characterization of protein protein interactions,were introduced.Generally speaking,the biochemical methods require the purification of proteins and (or) antibody preparation,whereas some molecular biological methods such as two hybrid system do not.The molecular biological methods were developed based on construction of gene libraries.Therefore,the coding sequence that interacts with a known protein can be directly deduced in comparison to the biochemical methods.This review emphasizes the principles,applications,advantages and disadvantages of the techniques mentioned above.

蛋白质间相互作用技术的研究近况黄翠芬叶棋浓(军事医学科学院生物工程研究所,北京100850关键词:蛋白质,相互作用,技术RecentAdvancesintheTechniquesofProtein┐ProteinInteractionsHuangCu...

BIA technology (biomolecular interaction analysis) is a new concept by using newly developed biosensor technology . The interaction of biomolecules can be monitored in real time and without the use of labels. BIA technology and its developed instrument BIACORE now are used in a wide range of application areas including signal transduction mechanisms, immune recognition, structure-function analysis, molecular biology, antibody characterization, neuroscience and drug development.

BIA技术(biomolecular interaction analysis)——生物分子相互作用分析技术是利用生物传感技术而发展的全新概念。生物分子间的相互作用可在免标记的状态得到实时的追踪和分析。目前的应用涉及到生物学结合分析、动力学及亲和力测定、免疫识别研究、结构与功能分析、基因调控、神经生物学和药物开发等多个领域。本文介绍BIA的原理和在生物各领域中的部分应用情况。

 
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