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Valve replacement represents the most common surgical therapy for valvular heart diseases. However, the long term results of the valve protheses in clinical use are not satisfying. Recently, with the development of tissue engineering, more and more studies are focusing on creating ideal valve substitutes through seeding autologous cells on biodegradable scaffold. This article describes the definition of valvular tissue engineering, the choice of scaffold biomaterials, the methods of cell culture and seeding,... Valve replacement represents the most common surgical therapy for valvular heart diseases. However, the long term results of the valve protheses in clinical use are not satisfying. Recently, with the development of tissue engineering, more and more studies are focusing on creating ideal valve substitutes through seeding autologous cells on biodegradable scaffold. This article describes the definition of valvular tissue engineering, the choice of scaffold biomaterials, the methods of cell culture and seeding, and the evaluation of tissue engineered heart valves. Furthermore, the problems which should be resolved in the future are also presented in the article. 心脏瓣膜置换术是外科治疗瓣膜性心脏病的主要方法 ,但目前临床应用的人工瓣膜的远期效果尚不满意。近年来 ,随着组织工程学技术的进展 ,利用培养的自身组织细胞种植于支架材料表面 ,体外重新构建理想的心脏瓣膜移植物日益成为研究热点。本文简述了心脏瓣膜组织工程的定义 ,细胞支架材料的选择 ,种子细胞的培养、种植方法以及组织工程化心脏瓣膜的评估 ,并指出下一步研究中尚需解决的问题。 Objective:Scaffold biomaterials for cell culture of tissue engineering demand satisfactory biocompatibility.This experiment aimed to evaluate biocompatibility of self designed scaffold biomaterial of NECM.Method:The biocompatibility of self designed NECM was evaluated both in vivo and in vitro,including cytotoxic test, acute and subacute systemic toxicity test, hemolysis test, pyrogenic test, lymphocyte transformation test and long term subcutaneous implantation test of NECM.Result:Self designed... Objective:Scaffold biomaterials for cell culture of tissue engineering demand satisfactory biocompatibility.This experiment aimed to evaluate biocompatibility of self designed scaffold biomaterial of NECM.Method:The biocompatibility of self designed NECM was evaluated both in vivo and in vitro,including cytotoxic test, acute and subacute systemic toxicity test, hemolysis test, pyrogenic test, lymphocyte transformation test and long term subcutaneous implantation test of NECM.Result:Self designed NECM scaffold biomaterial for cell culture has no cytotoxicity, no acute and subacute systemic toxicity, and it did not cause inflammatory reaction.Conclusion:Self designed NECM has good biocompatibility and may become an ideal biological scaffold material for cell culture of tissue engineering. 目的 :对自行研制的天然脱细胞基质 (NECM)进行一系列生物相容性试验 ,评价其组织相容性。方法 :采用细胞毒性试验、全身毒性试验、溶血试验、热原试验、淋巴细胞转化试验及异体体内植入试验来评价其生物相容性。结果 :自制NECM支架材料无细胞毒性 ,无溶血现象 ,无全身毒性 ,无致热源作用 ,无致敏淋巴细胞作用 ,体内埋植不引起炎症反应。结论 :自制NECM具有良好的生物相容性 ,有可能成为理想的天然生物支架材料。 AIM: To study the biocompatibility between two different biomaterials (collagen protein sponge and glutin sponge) and cultured hippocampus-derived neural stem cells in vitro and to identify whether the materials could be used as scaffold biomaterials in peripheral neural tissue engineering. METHODS: Hippocampus-derived neural stem cells of guinea pigs were cultured and respectively combined with each of the two biomaterials in vitro. The number of cells was counted and the histological changes were... AIM: To study the biocompatibility between two different biomaterials (collagen protein sponge and glutin sponge) and cultured hippocampus-derived neural stem cells in vitro and to identify whether the materials could be used as scaffold biomaterials in peripheral neural tissue engineering. METHODS: Hippocampus-derived neural stem cells of guinea pigs were cultured and respectively combined with each of the two biomaterials in vitro. The number of cells was counted and the histological changes were observed with HE staining and scanning electron microscope after 7 d and the adhesion rates were detected. RESULTS: Hippocampus-derived neural stem cells attached to both biomaterials and normally grew. The adhesion rates were 31.17% and (14.87%) respectively. The total numbers of the cells in the experimental and control groups were similar and there was no statistical difference. CONCLUSION: Both biomaterials have a good biocompatibility with hippocampus-derived neural stem cells of guinea pigs and can be used safely as scaffold materials in peripheral neural tissue engineering. 目的:评价豚鼠海马神经干细胞与2种不同载体材料(胶原蛋白海绵和明胶海绵)的生物相容性,探讨它们作为周围神经组织工程载体材料的可行性.方法:体外培养新生豚鼠海马神经干细胞,传至第3代,将密度为1×1010/L细胞分别与2种载体材料联合体外培养,1wk后取材,进行细胞计数,倒置相差显微镜及扫描电镜观察,并测定2种材料与细胞的吸附率.结果:神经干细胞可以在胶原蛋白海绵和明胶海绵上生长,逐渐黏附,细胞吸附率分别为37.17%和14.87%,统计学分析有显著差异;各实验组与对照组之间细胞总数统计学分析无显著差异.结论:胶原蛋白及明胶2种材料尤其是胶原蛋白,具有良好的神经干细胞相容性,可以作为周围神经组织工程的支架材料应用于临床.
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