助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   glycoprotein gene 的翻译结果: 查询用时:0.011秒
图标索引 在分类学科中查询
所有学科
生物学
畜牧与动物医学
基础医学
更多类别查询

图标索引 历史查询
 

glycoprotein gene
    很抱歉,暂未找到该词条的译词。
相关语句
  “glycoprotein gene”译为未确定词的双语例句
     Analysis of Glycoprotein gene of H9N2 AIV
     H9N2亚型禽流感病毒纤突蛋白基因的分析
短句来源
     Sequencing and variation analyzing of the cloned S1 fragment of spike glycoprotein gene of the SARS coronavirus
     SARS冠状病毒S蛋白S1片段基因克隆和变异分析
短句来源
     Methods The full-length glycoprotein gene of rabies virus CVS-N2c was inserted into plasmid pcDNA3.1. COS-7 cells were transfected with the recombinant plasmid pcDNA3.1/CVS-N2c GP and the expression of GP was determined by indirect immune fluorescent test.
     方法 构建狂犬病毒糖蛋白 (GP)的真核表达质粒pcDNA3.1 CVS N2cGP作为核酸疫苗 ,瞬时转染COS 7细胞 ,间接免疫荧光试验检测pcDNA3.1 CVS N2cGP的表达 ;
短句来源
     Expression of E2 Glycoprotein Gene of Hepatitis G Virus in Insect Cells
     庚型肝炎病毒包膜糖蛋白E2基因在昆虫细胞中的表达
短句来源
     Construction and Immune Character of Fusion Protein of Ubiquitin and GP5 Glycoprotein Gene of Porcine Reproductive and Respiratory Syndrome Virus
     PRRSV GP5蛋白与泛素融合蛋白基因重组质粒的构建与免疫特性研究
短句来源
更多       
  相似匹配句对
     The gene of the glycoprotein g13 of EHV- I was sequenced.
     本文分析了其膜糖蛋白g13基因的碱基序列,并由此推导其相应的氨基酸序列。
短句来源
     Expression of Rabies Virus Glycoprotein Gene in E. coli
     狂犬病病毒糖蛋白基因在原核细胞中的表达
短句来源
     The gene of PNGase F from F.
     为了获得大量廉价的N-糖酰胺酶F,本论文研究的主要内容为根据目的基因N-糖酰胺酶F的cDNA序列设计引物,以F.
短句来源
     Gene Sequences
     基因序列(英文)
短句来源
查询“glycoprotein gene”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。
  glycoprotein gene
Genotyping of Korean isolates of infectious hematopoietic necrosis virus (IHNV) based on the glycoprotein gene
      
In this study, 51 stool samples of calves from 10 Brazilian dairy farms were analysed by an RT-PCR that amplifies a 488-bp fragment of the hypervariable region of the spike glycoprotein gene.
      
Analysis of several glycoprotein gene mutants indicated that those carrying insertions near the start of genes encoding glycoproteins E and I were viable, but showed markedly diminished plaque areas.
      
The phylogenetic tree of 27 CSFV sequences based on the complete envelope glycoprotein gene (Erns-E2) displayed better resolution than that based on the complete open reading frame.
      
The S1 glycoprotein gene of IBV isolates were amplified by reverse transcriptase - polymerase chain reaction (RT-PCR) and analyzed by restriction fragment length polymorphism (RFLP) analysis.
      
更多          


Two recombinants of vaccinia virus ( Tian Tan strain ) containing the coding sequence of Challenge Virus Standard ( CVS ) rabiesvirus glycopro tein were produced by insertion of the glycoprotein gene into the vaccinia tk gene. The coding sequence was controled under the vaccinia early -late promoter p7.5. The recombinants differed slightly in its sequence upstream of the translation start codon and both express effectively mature glycoprotein of rabiesvirus, which was transported to the cell...

Two recombinants of vaccinia virus ( Tian Tan strain ) containing the coding sequence of Challenge Virus Standard ( CVS ) rabiesvirus glycopro tein were produced by insertion of the glycoprotein gene into the vaccinia tk gene. The coding sequence was controled under the vaccinia early -late promoter p7.5. The recombinants differed slightly in its sequence upstream of the translation start codon and both express effectively mature glycoprotein of rabiesvirus, which was transported to the cell membrane.Immunological assays and trial immunizations in animals showed that the molecular weight of non-fusion glycoprotein of rabiesvirus approximates 64kD. It react specifically with antibody against authentic glycoprotein of rabiesvirus. Each recombinant induced rabiesvirus neutralizing antibodies in mice and rabbits and protection against lethal street virus challenge.

把狂犬病毒标准攻击株(Standard challenge virus)的糖蛋白编码序列插入天坛株痘苗病毒的胸腺核苷激酶(TK)基因区,并置于痘苗病毒7.5k蛋白启动子(P7.5)的控制之下,构建了两株重组痘苗病毒。它们在起始密码子上游一侧的核苷酸序列稍有差别,但均能有效地表达狂犬病毒的成熟糖蛋白并能转运至细胞膜上。免疫学试验及动物试验结果表明,重组痘苗病毒表达的狂犬病毒非融合性糖蛋白分子量约为64kD;均能与狂犬病毒天然糖蛋白单克隆抗体发生特异性反应,并能在小鼠和家兔中诱生高滴度狂犬病毒中和抗体(VNA)并对致死量狂犬病毒攻击动物具有保护能力。

Two strains of recombinant vaccinia virus expressing rabies glycoprotein gene were inoculated into dogs by three ways. High tilers of neutralizing antibodies were induced by scarification and intramuscular routes, but there was no seroconversion when being immunized by oral route Immunologic effects were also observed when the recombinants were inoculated into mice by various ways.

用表达狂犬病毒糖蛋白的两株重组痘苗病毒对狗口服、划痕和肌肉注射三种途径的免疫效果进行观察。结果口服免疫未见抗体阳转,而划痕和肌注免疫在第七天就能诱导中和抗体。中和抗体滴度达642。以肌肉注射、皮下注射和脚掌注射免疫小鼠,亦获得较好的免疫效果。

Recombinant vaccinia virus carrying rabies glycoprotein gene (RTTVV-3) was passaged on chicken embryo cells, Blue plaque were present on agarose containg neutral red and X-gal. The virus titre was 3×10~7~4×10~7PFU/mL between 11th to 30th passage. A similar HI titer was found with the virus of differential passage. The molecular weight was 64 KD tested by Western bloting. The immunogenicity and protect effect of recombinant in mice were invariable affter different passage, The results above showed that...

Recombinant vaccinia virus carrying rabies glycoprotein gene (RTTVV-3) was passaged on chicken embryo cells, Blue plaque were present on agarose containg neutral red and X-gal. The virus titre was 3×10~7~4×10~7PFU/mL between 11th to 30th passage. A similar HI titer was found with the virus of differential passage. The molecular weight was 64 KD tested by Western bloting. The immunogenicity and protect effect of recombinant in mice were invariable affter different passage, The results above showed that the biological charactreistics.

将重组病毒在原代鸡胚细胞上连续传至30代(P30),其结果P30与第11代(P11)一样,在含中性红和X-gal的琼脂糖平板上为蓝色蚀斑;空斑法测定P11至P30,各代病毒滴度均稳定在3×10~7PFU/mL~4×10~7PFU/mL;血凝试验表明。不同代次的血凝滴度基本稳定在相同水平;Western blot检测P30与P11在同一位置出现相同蛋白带;血清中和试验证实传代后的重组病毒抗原活性及保护性稳定。重组病毒经传30代后,在生物学特性和表达水平上都具有良好的遗传稳定性。

 
<< 更多相关文摘    
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关glycoprotein gene的内容
在知识搜索中查有关glycoprotein gene的内容
在数字搜索中查有关glycoprotein gene的内容
在概念知识元中查有关glycoprotein gene的内容
在学术趋势中查有关glycoprotein gene的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社