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plaque forming cells
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  溶血空斑形成细胞
     Results Qubi granule (1 2, 2 4 g·kg -1 , ig, bid×10) improved the phagocytic function of mononuclear phagocytes, inhibited DTH, and increased the number of specific rosette forming cells (SRFC) and plaque forming cells (PFC).
     结果祛痹颗粒剂(12,24g·kg-1,ig,bid×10)能增强小鼠单核吞噬细胞的吞噬功能,抑制DTH,增加脾脏特异性玫瑰花结形成细胞和溶血空斑形成细胞数。
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     We applied iH Morphia 0.6mg/kg a time per 12h to the fracture stress rats. Then detected the number of the hemolytic plaque forming cells by liquid phase monophasic slide method and the number of the hemolysin antibody formation with spectrophotometry and excised the thymus glands and spleens and weighed them af ter 1,3,5,7 days.
     骨折应激性小鼠iH吗啡0.6mg/kg,每12小时一次,于应激后1、3、5、7天,用液相单层玻片法测溶血空斑形成细胞数(PFC),用分光光度法测定溶血素抗体生成数,并摘取胸腺和脾脏称重。
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  “plaque forming cells”译为未确定词的双语例句
     Plaque forming cells (PFC) were studied in spleen of mice immunized with sheep red blood cells (SRBC) after X-irradiation with doses of 0.25, 0.50, 1.0,2.0,3.0 and 4.0Gy.
     本实验观察了0. 25、0. 5、1. 0、2. 0、3. 0和4. 0Gy全身照射后3至6 h羊红细胞(SRBC)免疫对小鼠脾脏抗体形成细胞(PFC)的影响。
短句来源
     At the dose of 50 mg/kg·d ig×5d, AP also caused a significant increase of spleen plaque forming cells ( PFC ) in normal mice.
     当剂量为50mg/kg·d×5d,ig时,它还能显著增加正常小鼠的空斑形成细胞数。
短句来源
     At lower doses, CuB increased the number of peripheral blood T lymphocytes(0.1 mg/kg),the rate of PHA-induced lymphocyte transformation(0.2 mg/kg),the number of plaque forming cells(PFC)of the spleen(0.2 mg/kg)and the level of serum hemolysin(0.4 mg/kg).
     0.2 mg/kg 能提高 PHA 诱导的 T 淋巴细胞转化率和脾脏空斑形成细胞数; 0.4 mg/kg 时血清溶血素水平也明显增加。
短句来源
     The immune functions, such as phagocytosis of macrophage in the abdominal cavity, plaque forming cells and delayed type hypersensitivity etc. were examined at day 1, 3, 7, 15 and 30 following NNK injection.
     在注射 NNK后第 1、 3、 7、 15和 30天测定腹腔巨噬细胞吞噬率、脾抗体生成细胞数和迟发型变态反应等免疫功能指标。
短句来源
     METHODS To determine the plaque forming cells, interleukin 2 activity and T cell subset.
     [方法 ]采用溶血空斑试验、白细胞介素 2活性测定、T细胞亚群测定等方法 .
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  相似匹配句对
     (2)Glycyrrhizin greatly inhibited the forming of plaque units.
     (2)甘草甜素能明显减少空斑的形成。
短句来源
     Cell culture and plaque forming test of five arboviruses
     5种虫媒病毒的细胞培养及空斑试验
短句来源
     forming works;
     形成作品;
短句来源
     Cutting and Forming
     切削加工与塑性加工 两大加工方法的技术革新与市场变化
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     Plaque determination
     蚀斑测定
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  plaque forming cells
The filtrate abrogated completely the primary antibody response to both antigens as measured by the number of spleen hemolytic plaque forming cells when HP was administered at doses greater than 200 ?g two days before the antigen.
      
The influence of LK-409 on the number of B and T cells in spleen and the number of peritoneal macrophages was studied; Jerne's plaque forming cells assay was performed to monitor the effect of B cell differentiation.
      
Both aprotinin and ovomucoid diminished a secondary response to SRBC, limited to the indirect plaque forming cells.
      
pertussis', cultured spleen cells from treated mice were measured for their ability to produce plaque forming cells (PFC), in vitro, and to participate in mixed cell interactions, in vitro.
      
injections of sheep erythrocytes into mice, the following parameters of the immune response were evaluated: splenic cell count, 19S plaque forming cells, 7S plaque forming cells and 2-mercaptoethanol-sensitive and-resistant haemagglutinin.
      
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A modified method of Jerne's assay was used to determine haemolytic plaque-forming cells (PFC) in mouse spleen. By means of this method we measured the dynamic changes of the immune status in tumor-bearing mice. The tumors used were solid Sarcoma 180 maintained in Tian-jin albino mice, four ascitic tumors including Sarcoma 180 maintained in Tian-jin albino and 615 strain mice, and Hepatoma and Ehrlich carcinoma maintained in C57BL mice. The results. showed that spleen PFC decreased as the tumors...

A modified method of Jerne's assay was used to determine haemolytic plaque-forming cells (PFC) in mouse spleen. By means of this method we measured the dynamic changes of the immune status in tumor-bearing mice. The tumors used were solid Sarcoma 180 maintained in Tian-jin albino mice, four ascitic tumors including Sarcoma 180 maintained in Tian-jin albino and 615 strain mice, and Hepatoma and Ehrlich carcinoma maintained in C57BL mice. The results. showed that spleen PFC decreased as the tumors progressed, and was hardly detected when the tumors were far advanced. The tumor-bearing mice commonly had striking splenomegaly with. decrease in number of small lymphocytes and. increase of 'immature' plasma cells, which might explain the poor immune responses of tumor-bearing mice.

本文应用改良的Jerne氏方法测定小鼠脾脏溶血空斑形成细胞。用此方法测定一株实体瘤(在津白小鼠上移植之肉瘤180)和四株腹水瘤(津白小鼠上移植之肉瘤180,615小鼠上移植之肉瘤180,以及C57BL小鼠上移植之肝癌和艾氏癌)带瘤动物免疫状态的动态变化。结果表明,随着肿瘤进行性生长,小鼠脾脏溶血空斑形成细胞逐渐减少,到晚期几乎不能测得。带瘤小鼠的脾脏普遍增大,病理组织学观察提示带瘤小鼠溶血空斑反应之低下与其脾脏的病理学改变相关。

It has been shown by haemolytic plaque-forming cell assay that there is an immunosuppressive factor(s) with high activity in the cell-free aseitic fluid of Sarcoma 130 maintained in 615 strain mice. The suppression is dose-dependent and becomes apparent only when the aseitic fluid is administered before immunization with sheep red blood cells. The immunosuppressive factor (s) seems partially heat-labile. The serum of the same tumor-bearing mice also possesses a strong immunosuppressive...

It has been shown by haemolytic plaque-forming cell assay that there is an immunosuppressive factor(s) with high activity in the cell-free aseitic fluid of Sarcoma 130 maintained in 615 strain mice. The suppression is dose-dependent and becomes apparent only when the aseitic fluid is administered before immunization with sheep red blood cells. The immunosuppressive factor (s) seems partially heat-labile. The serum of the same tumor-bearing mice also possesses a strong immunosuppressive activity. Furthermore, similar immunosuppressions were observed in normal recipient mice treated with cell-free aseitic fluids and sera of mice bearing some other strains of tumor including Hepatoma and Ehrlich carcinoma maintained in C57BL mice and Sarcoma 180 maintained in Tian-jin albino mice. Histopathologic changes of spleens induced by the cell-free aseitic fluid resembled those observed in spleens of tumor-bearing mice.

用溶血空斑试验证明,在615小鼠上移植的肉瘤180的无细胞腹水液中存在着高活性的免疫抑制因子。腹水液的免疫抑制作用与剂量相关,且仅在绵羊红血球抗原致敏前注射才能显示。此免疫抑制因子具有部分热不稳定的性质。肉瘤180带瘤小鼠的血清也有强免疫抑制活性。在C57BL小鼠上移植的肝癌和艾氏癌,在津白Ⅰ号小鼠上移植的另一株肉瘤180的腹水液和带瘤动物血清也有类似的免疫抑制作用。肿瘤无细胞腹水液引起的小鼠脾脏病理组织学变化与带瘤动物脾脏所见相类似。

The spleen cells taken from Balb/ cJ mice 3 days after immunization with PDRBC were fused with NS-1 myelo-ma cells by two different procedures in the presence of PEG.The hybridcells secreting the specific antibody were selected out in the HAT medium by means of the spot test of the culture fluid.(Table 1).The antibody secre-ting hybrid cells were then cloned by either...

The spleen cells taken from Balb/ cJ mice 3 days after immunization with PDRBC were fused with NS-1 myelo-ma cells by two different procedures in the presence of PEG.The hybridcells secreting the specific antibody were selected out in the HAT medium by means of the spot test of the culture fluid.(Table 1).The antibody secre-ting hybrid cells were then cloned by either limiting dilution or micromani-pulation methods and a cell line thus obtained was designated as CBH-1.After ten months of culture in vitro up to 69 generations,the ability of this cell line to secret specific monoclonal antibody to PDRBC was not decreased. The CBH-1 cells were also ino-culated into the peritoneal cavity of Balb/cJ or Balb/cCr mice and could be kept in these mice for at least four transplantation generations.The ability of three different agents for inducing the ascitic fluid was compared.As a result,pristane was found to be superior to liquid paraffin and Arlacel A. Attempt was also made to cultureCBH-1 in serum-free medium instead of the conventional medium containing 20% newborn calf serum (NCS).When the serum-free medium was provided with 5γ transferrin per ml,the CBH-1 cells could be maintained just as well as that in DMEM+20% NCS within 15 days (Table 2) The proportion of plaque forming cells after the first cloning was found to be 97.5% and this was increased to 99.8% after second cloning (Table 3).This result implies that the cell popula-tion of the CBH-1 clone was very ho-mogeneous with respect to the secre-tion of the specific antibody to PDR-BC.

以北京鸭红血球为抗原免疫Balb/cJ小鼠,三天后取脾细胞与小鼠NS-1骨髓瘤细胞在PEG作用下,用两种方法进行细胞融合。只有淋巴细胞杂交瘤细胞能在一种选择性培液——HAT液中生长,具有分泌抗体活性的杂交瘤细胞,借助其上清液的斑点试验加以选择。对斑点试验阳性的杂交瘤细胞进行克隆化和扩大培养,得到能专一分泌抗北京鸭红血球单克隆抗体的淋巴细胞杂交瘤,定名为CBH-1。在体外经过69代培养,历时十月余,分泌能力仍未见衰减。体内可传4—5代,腹水中可检出大量分泌抗体。用降植烷、Arlacel A和石蜡油诱发动物的腹水,其量以降植烷最高、石蜡油次之。使用国产石蜡油作为诱发腹水试验似具有一定实用价值。加5γ转铁蛋白至每毫升不含牛血清的DMEM液中,在15天的培养期内,结果至少与加20%小牛血清的培液的结果相似。首次克隆化后的CBH-1细胞中,空斑形成细胞达97.5%,再次克隆化的细胞中,空斑形成细胞达99.8%,证明是均一的细胞群落。本文还就杂交瘤制备过程及细胞稳定性问题进行了讨论。

 
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