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verticillium wilt pathogen
相关语句
  黄萎病菌
     Studies on methods of gDNA extraction and RAPD analysis of cotton Verticillium wilt pathogen
     棉花黄萎病菌gDNA提取方法和不同单孢的RAPD分析
短句来源
     IDENTIFICATION OF HOSTS OF THE COTTON VERTICILLIUM WILT PATHOGEN(VERTICILLIUM DAHLIAE KLEB.)
     棉花黄萎病菌(Verticillium dahliae K1eb.)的寄主种类鉴定
短句来源
     Comparison on Biological Characteristics of Different Pathogenicity Strains of the Verticillium Wilt Pathogen of Eggplant
     茄子黄萎病菌不同致病力菌株生物学特性的比较
短句来源
     RAPD Patterns Characteristic for VCGs of Cotton Verticillium Wilt Pathogen
     棉花黄萎病菌营养体亲和群的RAPD图谱特征
短句来源
     Promising production of the toxin of the Verticillium wilt pathogen was obtained with an incubation of 15 days at 25℃ with czapek culture under laboratory condi-tions.
     实验室培养棉花黄萎病菌,用查彼克培养基,在25℃下培养15天是病菌致萎毒素产生的适宜条件。
短句来源
更多       
  棉花黄萎病菌
     IDENTIFICATION OF HOSTS OF THE COTTON VERTICILLIUM WILT PATHOGEN(VERTICILLIUM DAHLIAE KLEB.)
     棉花黄萎病菌(Verticillium dahliae K1eb.)的寄主种类鉴定
短句来源
     Studies on methods of gDNA extraction and RAPD analysis of cotton Verticillium wilt pathogen
     棉花黄萎病菌gDNA提取方法和不同单孢的RAPD分析
短句来源
     RAPD Patterns Characteristic for VCGs of Cotton Verticillium Wilt Pathogen
     棉花黄萎病菌营养体亲和群的RAPD图谱特征
短句来源
     Promising production of the toxin of the Verticillium wilt pathogen was obtained with an incubation of 15 days at 25℃ with czapek culture under laboratory condi-tions.
     实验室培养棉花黄萎病菌,用查彼克培养基,在25℃下培养15天是病菌致萎毒素产生的适宜条件。
短句来源
  “verticillium wilt pathogen”译为未确定词的双语例句
     Inhibition Effects on Proteins Extracted From Cotton,Pokeweed and Gastrodia to Cotton Fusarium and Verticillium Wilt Pathogen
     棉花、商陆和天麻蛋白提取物对棉枯、黄萎菌离体抑制试验
短句来源
     DNA extracted by CTAB method has longer fragment, higher purity and better stability. So it is suitable for the DNA extraction of cotton Verticillium wilt pathogen. The RAPD analysis has been performed with 26 cotton Verticillium strains in 40 primers (OPY, OPB), 12 primers amplified polymorphic DNA patterns.
     在提取我国12个省主产棉区26个菌系gDNA的基础上,采用OPY和OPB两组随机引物,对这些菌系的基因组进行了PCR扩增和聚类分析,40个引物中,12个引物扩增出满意的适合于分析的多态性谱带。
短句来源
     The result of RAPD analysis of 4 Anyang strains showed the correlation of pathogenicity of cotton Verticillium wilt pathogen with RAPD analysis.
     不同的单孢之间RAPD分析的结果(引物OPY06检测出单孢之间2.06kb的差异条带)从分子水平证实了单孢间变异的发生。
短句来源
     Study was made on biological characteristics of V.dahliae of different pathogenicity strains of the verticillium wilt pathogen of eggplant which were isolated from the diseased stems in the suburb of Yaan,Sichuan.
     在雅安近郊茄园中,采集黄萎病症状典型的茄株,进行了病原菌的分离鉴定,并研究了病原菌的致病力、生物学特性。
短句来源
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Promising production of the toxin of the Verticillium wilt pathogen was obtained with an incubation of 15 days at 25℃ with czapek culture under laboratory condi-tions. Main substances inducing cotton wilt were known as proteins composing of glycoproteins of 17 kinds of amino acids with 21.2% of which belonging to acidic amino acids and 8.9% of which being basic amino acids. 13 protein bands had deen separated by electrophoresis. With seedlings of cotton varieties Central Cotton No.3 and RF-1 immerged...

Promising production of the toxin of the Verticillium wilt pathogen was obtained with an incubation of 15 days at 25℃ with czapek culture under laboratory condi-tions. Main substances inducing cotton wilt were known as proteins composing of glycoproteins of 17 kinds of amino acids with 21.2% of which belonging to acidic amino acids and 8.9% of which being basic amino acids. 13 protein bands had deen separated by electrophoresis. With seedlings of cotton varieties Central Cotton No.3 and RF-1 immerged in the dilution containing phytotoxins for as long as 65 hrs. , the severity indices mounted to 70.45 and 47.73 accordingly.

实验室培养棉花黄萎病菌,用查彼克培养基,在25℃下培养15天是病菌致萎毒素产生的适宜条件。病菌危害棉花导致棉株凋萎死亡的毒素物质主要是蛋白质,其组份多为糖蛋白,含有17种氨基酸,其中酸性氨基酸占21.2%,碱性氨基酸占8.9%,电泳分离出13条蛋白质条带。棉花品种中棉所10号和FR-1棉苗在病菌毒素稀释液内浸至65小时,病情指数分别达到70.45和47.73。

This report showes the present advances of cotton Verticillium wilt pathogens in the identification of species, physiological type and physiological race, vegetative compatibility, morphological change, host range, distribution of soil, pathogenesis mechanism, physiologic biochemical, isoenzyme analysis and molecular biology, etc.

详述了棉花黄萎病菌从发展(1914年)至目前,在种、生理型、生理小种的鉴定,营养体亲和性,形态及变异,寄主范围,土壤分布及监测,致病机制,生理生化,同功酶分析及分子生物学等方面的研究及最新进展。

DNA extracted by CTAB method has longer fragment, higher purity and better stability. So it is suitable for the DNA extraction of cotton Verticillium wilt pathogen. The RAPD analysis has been performed with 26 cotton Verticillium strains in 40 primers (OPY, OPB), 12 primers amplified polymorphic DNA patterns. By the cluster analysis, 26 strains are divided into 2 clusters (A,B). V. alboatrum belong to cluster A, cluster B comprises the other Verticillium strains (25 strains); cluster B can...

DNA extracted by CTAB method has longer fragment, higher purity and better stability. So it is suitable for the DNA extraction of cotton Verticillium wilt pathogen. The RAPD analysis has been performed with 26 cotton Verticillium strains in 40 primers (OPY, OPB), 12 primers amplified polymorphic DNA patterns. By the cluster analysis, 26 strains are divided into 2 clusters (A,B). V. alboatrum belong to cluster A, cluster B comprises the other Verticillium strains (25 strains); cluster B can be divided into 2 subclusters including Ba and Bb, subcluster Bb followed by 3 groups: Bb1, Bb2, Bb3. The group Bb3 is the largest group, including defoliating strains (T 9, V D-8 , V 44 ) and majority of Verticillium dahliae strains. The result of RAPD analysis of 4 Anyang strains showed the correlation of pathogenicity of cotton Verticillium wilt pathogen with RAPD analysis. RAPD analysis of different single spores also proved that the genomic variation may be occur among different single spores from one species.

在提取我国12个省主产棉区26个菌系gDNA的基础上,采用OPY和OPB两组随机引物,对这些菌系的基因组进行了PCR扩增和聚类分析,40个引物中,12个引物扩增出满意的适合于分析的多态性谱带。聚类分析将26个菌系分为A、B两个群,Va菌系(Verticiliumalboatrum)属于单独的A群,B群则包括了所有的大丽轮枝菌系(Verticiliumdahliae)。B群又分为Ba(Cs、Yc)及Bb两个亚群。Bb亚群下面分3个组,Bb1(AV2、AV3及Ly)、Bb2(Sa、Ji、Ke及Yc)和Bb3。Bb3是最大的一个组,包括供试的3个大丽轮枝菌落叶型的3个菌系(VD-8、T9、V44)和其他大部分非落叶型的菌系。不同的单孢之间RAPD分析的结果(引物OPY06检测出单孢之间2.06kb的差异条带)从分子水平证实了单孢间变异的发生。对河南安阳4个菌株单独聚类分析则表现出致病性测定与RAPD分析有一定的相关性。

 
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