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breast cancer lines
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  人乳腺癌细胞株
     The significance of expressions of cyclooxgenase-2 in human or rat breast cancer and breast cancer lines in Vitro
     环氧化酶-2在人、大鼠乳腺癌及人乳腺癌细胞株的表达及其意义
短句来源
     Objectives:To determine the effect of folacin deficiency on the chemotherapy of breast cancer lines MCF 7 and breast cell line HBL 100. Methods:These two cell lines were cultured for 3 days in the presence of chemotherapeutics.
     目的 :检测叶酸缺乏时化疗药物对人乳腺细胞株HBL 1 0 0和人乳腺癌细胞株MCF 7敏感性的影响。 方法 :将HBL 1 0 0和MCF 7在正常培养液和叶酸缺乏培养液中加入化疗药物 ,培养 3天 ,用四唑蓝 (MTT)法检测化疗药物对HBL 1 0 0和MCF 7的杀伤作用。
短句来源
     Objective To investigate the significance of expressions of cyclooxygenase-2 (COX-2) in human or rat breast cancers and human breast cancer lines in vitro.
     目的探讨环氧化酶-2(COX-2)在人、大鼠乳腺癌及人乳腺癌细胞株的表达及其意义。
短句来源
     Methods Immunohistochemistry, RT-PCR, Western blot assays were used to assess the expressions of COX-2 in cancerous tissues, paired tissues adjacent to breast cancer, normal tissues far from neoplasms, including human, rat breast cancers, and two kinds of human breast cancer lines.
     方法应用免疫组织化学、Westernbloting、RT-PCR法检测人乳腺癌组织及配对的癌旁组织、大鼠致癌组、选择性COX-2抑制剂尼美舒利(NIM)组、人乳腺癌细胞株中COX-2的表达,MMT法检测细胞株的生长率。
短句来源
  乳腺癌细胞株
     Objective To investigate the significance of expressions of cyclooxygenase-2 (COX-2) in human or rat breast cancers and human breast cancer lines in vitro.
     目的探讨环氧化酶-2(COX-2)在人、大鼠乳腺癌及人乳腺癌细胞株的表达及其意义。
短句来源
     Objectives:To determine the effect of folacin deficiency on the chemotherapy of breast cancer lines MCF 7 and breast cell line HBL 100. Methods:These two cell lines were cultured for 3 days in the presence of chemotherapeutics.
     目的 :检测叶酸缺乏时化疗药物对人乳腺细胞株HBL 1 0 0和人乳腺癌细胞株MCF 7敏感性的影响。 方法 :将HBL 1 0 0和MCF 7在正常培养液和叶酸缺乏培养液中加入化疗药物 ,培养 3天 ,用四唑蓝 (MTT)法检测化疗药物对HBL 1 0 0和MCF 7的杀伤作用。
短句来源
     The significance of expressions of cyclooxgenase-2 in human or rat breast cancer and breast cancer lines in Vitro
     环氧化酶-2在人、大鼠乳腺癌及人乳腺癌细胞株的表达及其意义
短句来源
     Methods Immunohistochemistry, RT-PCR, Western blot assays were used to assess the expressions of COX-2 in cancerous tissues, paired tissues adjacent to breast cancer, normal tissues far from neoplasms, including human, rat breast cancers, and two kinds of human breast cancer lines.
     方法应用免疫组织化学、Westernbloting、RT-PCR法检测人乳腺癌组织及配对的癌旁组织、大鼠致癌组、选择性COX-2抑制剂尼美舒利(NIM)组、人乳腺癌细胞株中COX-2的表达,MMT法检测细胞株的生长率。
短句来源
     PART ONE The impact of integrin αvβ3 on cell adhesion and migration in breast cancer lines
     [目的]检测乳腺癌细胞株MCF7、MDA-MB-231、MDA-MB-435s整合素αvβ3的表达状况,通过细胞粘附、迁移实验了解整合素αvβ3对乳腺癌细胞的作用。
短句来源
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  株乳腺癌细胞株
     Methods:The effects of HSP90 inhibitor,Geldanamycin(GA),on the adhesion to artificial basement membrane,Matrigel of breast cancer lines were assayed by MTT methods.
     方法:MTT法检测HSP 90抑制剂geldanamycin(GA)对2株乳腺癌细胞株粘附基质胶能力的影响;
短句来源
  “breast cancer lines”译为未确定词的双语例句
     Results 10 -10 ~10 -7 mol/L vitamin D 3 strongly inhibited proliferation of the 2 breast cancer lines, while 10 -9 ~ 10 -7 mol/L tamoxifen stimulated proliferation of MCF 7 cell.
     结果(10 -10 ~ 10 -7)mol/L维生素D3 对MCF 7及ZR 75 1细胞呈现明显的抑制生长作用 ,并能抑制 (10 -9~10 -7)mol/L三苯氧胺对MCF 7细胞的刺激生长作用。
短句来源
     Conclusion:HSP expression patterns were associated with the adhesive and invasive abilities of breast cancer lines.
     结论:HSP表达特性与乳腺癌细胞的粘附、侵袭能力相关;
短句来源
     EFFECT OF RUNING NO.2 ON THE GROWTH OF MCF-7 HUMAN BREAST CANCER LINES IN VIVO AND IN VITRO
     中药乳宁Ⅱ号对人乳腺癌MCF-7细胞株体内外生长的影响
短句来源
     Consistent with HSP expression patterns in these two cell lines, MDA-MB-435s were inhibited more by GA than MDA-MB-231.Results had shown high expression of HSP90 in MDA-MB-435s and HSP27 in MDA-MB-231.It demonstrated that the HSP expression patterns were associated with the invasive and metastatic abilities of breast cancer lines.
     MDA-MB-231 细胞实验组的侵袭迁移能力明显低于对照组,差异有显
短句来源
     Anti-cyclinD1therapy is necessary for patients with activated neu-ras pathway. p16is a new tumor suppressor gene . The deletion and mu tation hasbeen found in breast cancer lines.
     CyclinD1在多个癌基因调控通路上有重要作用,对有激活的neu-ras通路的乳腺癌病人,有必要给予抗CyclinD1治疗。
短句来源
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  breast cancer lines
In summary, MV-CEA has potent antitumor activity against breast cancer lines and xenografts.
      
All breast cancer lines overexpressed the measles virus receptor CD46 and supported robust viral replication, which correlated with CEA production.
      
In two further breast cancer lines (T47D and HS578T) PKB/Akt phosphorylation was dependent upon autocrine factors acting primary through the epidermal growth factor receptor (EGFR) and erbB2.
      
The Expression of Growth Hormone-Releasing Hormone (GHRH) and its Receptor Splice Variants in Human Breast Cancer Lines; The Eva
      
Ceramide glycanase (CGase) activities have been detected in different human tumor cells (colon, carcinoma Colo-205; neuroblastoma, IMR-32; breast cancer lines, SKBr3 and MCF7).
      
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Three hybridoma lines secreting McAbs against the lung cancer cell line (LTEP-78) have been established. One of them, BLTI-01, has been subcloned six times and cultured more than eight months in vitro.The experimental data showed that the BLTI-01 has no crossreaction with human lymphocyte surface Ags (within 140 samples) , human erythrocyte surface Ags (100 samples) , marrow cell surface Ags (10 samples) , CEA and AFP. Further experiments with 24 human normal and tumour cell lines indicated that the McAb from...

Three hybridoma lines secreting McAbs against the lung cancer cell line (LTEP-78) have been established. One of them, BLTI-01, has been subcloned six times and cultured more than eight months in vitro.The experimental data showed that the BLTI-01 has no crossreaction with human lymphocyte surface Ags (within 140 samples) , human erythrocyte surface Ags (100 samples) , marrow cell surface Ags (10 samples) , CEA and AFP. Further experiments with 24 human normal and tumour cell lines indicated that the McAb from BLTI-01 has strong positive reaction with three lung cell lines, one liver cancer, one osteoblastic sarcoma and one breast cancer lines, on the contrary, it hardly reacts with four normal tissue cell lines and the ther human caneer lines.We suggest that the corresponding Ag of McAb from BLTI-01 is a tumour associated Ag.

用人肺鳞癌细胞LTEP-78细胞系免疫Blab/c小鼠获得3株抗人肺癌细胞的单克隆抗体杂交瘤系。其中BLTI-01株经六次克隆化培养,体外传代8个月以上。BLTI-01与白细胞抗原及血型抗原基本上无交叉反应;与骨髓细胞无交叉反应;与癌胚抗原和胎甲球蛋白不相关;与肺鳞癌、肺腺癌细胞系及部分其它肿瘤细胞呈阳性反应。

HD1 is a hybridoma secreting monoclonal antibodies (IgG1) against human breast cancer, It was raised in our laboratory, ABC and indirect enzyme staining to detect the reactivity with varied cell lines, breast cancer and normal tissue sections were used. The results showed that HD1 reacted strongly to breast cancer lines (MCF -7, 475-A and Cap-37), but not to other human malignant cell lines and normal cells, and that HDl also reacted positively to maliganant breast cells in some tissue sections,...

HD1 is a hybridoma secreting monoclonal antibodies (IgG1) against human breast cancer, It was raised in our laboratory, ABC and indirect enzyme staining to detect the reactivity with varied cell lines, breast cancer and normal tissue sections were used. The results showed that HD1 reacted strongly to breast cancer lines (MCF -7, 475-A and Cap-37), but not to other human malignant cell lines and normal cells, and that HDl also reacted positively to maliganant breast cells in some tissue sections, not to normal cells in histalogical sections. The constants of affinity of HDl for the three kinds of breast cancer cells were among (1. 3-2.7) X 10-2 M. Western blot analysis showed that HDl recognized both MCF-7 and -175-A. A cell membrane, antigen band whose molecular weight was 60000 and to BCap-37 weae 56000 and 94000. The activity of ADCC to the three breast cell lines was among 18%-27%. These results indicate that there is possibility of clinical application of HD1 to diagnosis and therapy for breast cancer in the future.

HD1是由1株抗人乳腺癌杂交瘤腹水扩增,亲和层析纯化得到的单克隆抗体,分泌抗体(IgG)的蛋白含量:上清为10μg/ml,腹水为1.5mg/ml。用ABC法检测,HD1与3株人乳腺癌细胞系呈阳性反应,而与胃癌、结肠癌等细胞和正常成纤维细胞、淋巴细胞呈阴性反应。组织化学染色显示对部分乳腺癌石蜡切片为阳性,而对多种正常组织为阴性。HD1对3株乳腺癌细胞系的亲和力在1.3×10~(-8)~2.7×10~(-8)mol/L之间。免疫印迹实验表明,HD1可使乳腺癌细胞MCF-7和475-A细胞膜Mr为60000的抗原组分染色,使另一株乳腺癌细胞BCaP-37 Mr为56000和94000的组分着色。对3株乳腺癌细胞有抗体介导的细胞毒效应(18%~27%)。以上的研究有助于对HD1性质及其相对应抗原的了解,为抗乳腺癌单抗的应用提供了可靠的依据

We introduce a method of detecting the Estrogen Recoptor and ProgesteroneReceptor in human breast cancer by isoelectric focusing in flat beds of agarose gel.Owing to the very small amount of tissue(50mg)required in this method,it can be usedto detect the ER the PR content not only in mastectomy tissues,but also in the needlebiopsies. We detected the ER and PR content on thirty-nine cases of breast cancer andone MCF-7 breast cancer line by this method with the results aualogous to...

We introduce a method of detecting the Estrogen Recoptor and ProgesteroneReceptor in human breast cancer by isoelectric focusing in flat beds of agarose gel.Owing to the very small amount of tissue(50mg)required in this method,it can be usedto detect the ER the PR content not only in mastectomy tissues,but also in the needlebiopsies. We detected the ER and PR content on thirty-nine cases of breast cancer andone MCF-7 breast cancer line by this method with the results aualogous to that of DCCmethod. The technique procedure is very simple and well repeatable and allows severalkinds of hormonal receptors to be determined at the same time.

本文重点介绍一种琼脂糖凝胶等电聚焦电流(IEF法)对乳腺癌组织雌激素受体(ER)及孕酮受体(PR)的测定方法。鉴于该检测法所需组织样品量小,不仅可对手术切除组织进行测定,还能应用于活检标本。本文通过39例人体乳腺癌标本及1株MCF-7乳腺癌细胞株的检测,采用IEF法测定ER、PR,其所需组织量仅50mg。此法操作简便,实验重复性好,并能同时进行多种激素受体的测定。

 
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