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Two bacteria were isolated from waste-water of texitileindustries. One of them is Plesiomonas sp that can decolorize five azo dyes under anaerobic and static conditions to produce reduction products(aromatic amines) which can't be degraded by this bacterium, and this bacterium decolorizes azo dyes very slowly under aerobic conditions. Another bacterium isolated is Achromobacter sp. that can greatly reduce the amounts of aromatic amines which are reduction products of azo dyes by Plesiomonas sp.... Two bacteria were isolated from waste-water of texitileindustries. One of them is Plesiomonas sp that can decolorize five azo dyes under anaerobic and static conditions to produce reduction products(aromatic amines) which can't be degraded by this bacterium, and this bacterium decolorizes azo dyes very slowly under aerobic conditions. Another bacterium isolated is Achromobacter sp. that can greatly reduce the amounts of aromatic amines which are reduction products of azo dyes by Plesiomonas sp. 从印染厂排出水中分离出了两种细菌。其中的邻单胞菌Plesiomonas sp.X_(13)菌株对五种偶氮染料均有脱色作用。脱色率达75%以上;脱色产物中有芳香胺,但X_(13)菌株对苯胺在振荡培养或深层静止培养条件下均无降解能力。另一种,无色细菌Achromobacter sp.A_8菌株对脱色过程中产生的芳香胺则具有降解作用,其降解能力也因培养条件而异。在振荡培养条件下,其降解芳香胺的速率远比静止培养时为快。 There are obvious shortages in the research of the fundamental theories of LABS biodegradation up to date. From the microbial ecological and physisological point of view, the authors recommended the ecological adaption of microbial community to the nonbiogenesis substance LABS and the characteristics of LABS biodegradation by the bacteria screened. It has been found that many kinds of bacteria have the ability of degrading LABS and that most of them have LABS biodegradation plasmid which encloses some important... There are obvious shortages in the research of the fundamental theories of LABS biodegradation up to date. From the microbial ecological and physisological point of view, the authors recommended the ecological adaption of microbial community to the nonbiogenesis substance LABS and the characteristics of LABS biodegradation by the bacteria screened. It has been found that many kinds of bacteria have the ability of degrading LABS and that most of them have LABS biodegradation plasmid which encloses some important enzymes for biodegradation. The bacteria screened can use LABS as sole carbon source, so that biodegrade LABS in wastewater with high efficiency. Plesiomonas sp. 90-1 immobilized by sodium alginate can biodeg rade more than 90% of 100 ppm LABS easily, which makes the aerobic treatment of high concentration of LABS possibly. 从微生物生态学和生理学等多角度地介绍了微生物群落对LAS这种非生物源物质的生态适应,以及筛选蒲株降解LAS的能力;研究表明,大多数细菌的降解能力,取决于降解性质粒的存在。筛选菌株以LAS为唯一碳源生长,可高效地去除污水中的LAS。用海藻酸钠固定化的筛选菌株PLesiomonas sp.90-1,还可高效降解100ppm的LAS废水,去除率在90%以上,实现了高浓度LAS废水的好氧处理。 After immobilised by alginate-Na, the abilities of tolerance and degradation of 100ppm LAS of Plesiomonas sp. 90-1 were increased obviously. We studied the cell's optimal immobilization conditions and the immobilised cell's Vm and Km were 435 m/ h, 1061 m, respective ly. We also studied the re-cultural conditions by using orthogonal tests, Under such optimal conditions the degradative ability of immobilised cellis can be increased by 116%. 邻单胞杆菌(Plesiomonas sp.90-1)经海藻酸钠固定后,忍耐和降解100 ppm的高浓度LAS的能力较同样浓度的游离细胞分别提高4.875倍和0.77倍。我们研究了最佳固定条件和固定化细胞的最佳降解条件。该固定化细胞米氏常数Km和最大反应速度V_m分别为1061μmol和43.5μmol/h。此外,利用多因子正交法确定了降解酶活恢复的最佳培养条件,在此条件下培养的固定化细胞的降解酶活可提高116%。
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