Methods:Wistar rats with acute hepatic failure (AHF group, n=30) were induced by intraperitoneal injection of D-glactosamine/endotoxin, normal controls (N group, n=30) were injected with saline solution.
Objective To investigate the effect of allogeneic hepatocyte transplantation (HcT) plus augmenter of liver regenration (ALR) intraperitoneally on acute hepatic failure (AHF) induced by D-galactosamine (D-gal) in rats.
目的探讨同种异体肝细胞腹腔移植联合肝再生增强因子(augmenter of liver regenration,ALR)对D-氨基半乳糖(D-gal)诱导的急性肝功能衰竭大鼠的治疗作用。
Objective To evaluate the protective effect of recombinant human granulocyte colony stimulating factor (rhG-CSF) on acute hepatic failure induced by galactosamine (D-GalN) and lipopolysaccharide (LPS) in mice, and to explore its mechanism.
目的:观察重组人粒细胞集落刺激因子(recombinant human granulocyte colony stimulating factor;rhG-CSF)对D-氨基半乳糖(D-galactosamine,D-GalN)加脂多糖(lipopolysaccharide,LPS)联合诱导的小鼠急性肝衰竭的保护作用,并探讨其机制。
With verified rather good effect of Qingkai Granule on acute hepatic failure in rats in previous study, acute hepatic failure model in mice is established to investigate roles of hepatic cells apoptosis and caspase-3in fulminant hepatitis and action mechanism of Qingkai Granule against acute hepatic failure.
Results(1)Different extent liver injury can be induced by different dosages of D-gal,and the pathophysiology of acute hepatic failure can be copied very well at the dose of 1.4g/kg,and the model is very steady.