Methods: After mice were orally administered 0.6,1.2,3.6 g/(kg·d) ETM and sterile water for 30 days,changes on weight,index of immune organs,proliferation ability of lymphocytes,NK cell activity, plaque-forming cell(PFC) response assayed by experiment of dissolving SRBC,phagocytic function of macrophages were observed.
The effect of exposure to tritiated water on Plaqu-forming cell(PFC)in spleen of BALB/cmice were studied with the liquid-phase monolayer plaque-forming cell technique.
Autologous mixed lymphocyte reaction of Tactive-T (TA-T AMLR) and the modulating effect of plaque-forming cell (PFC) on self-lymphocyte B cell in chronic active hepatitis B (CAH, n-10) were studied with cell culture assay. The results showed that PFC inhibitory rate of CAH was significantly lowered, it was strongly associated with its TA-T AMLR.
Thus, impaired regulation of Epstein-Barr virus-induced plaque-forming cell generation is a common feature of autoimmune disease and demonstrates some specificity for these disorders.
In contrast, peripheral blood mononuclear cells from scleroderma had defective suppression of both Epstein-Barr virus-induced proliferation and plaque-forming cell generation.
Peripheral blood mononuclear cells from rheumatoid arthritis had excessive plaque-forming cell generation in the face of a normally regulated decrease in Epstein-Barr virus-induced proliferation.
We examined regulation of Epstein-Barr virus-induced plaque-forming cell generation in peripheral blood mononuclear cells from several autoimmune and seronegative diseases and correlated these results with Epstein-Barr virus-induced proliferation.
As expected, repeated stresses reduced the plaque-forming cell (PFC) response.
A modified method of Jerne's assay was used to determine haemolytic plaque-forming cells (PFC) in mouse spleen. By means of this method we measured the dynamic changes of the immune status in tumor-bearing mice. The tumors used were solid Sarcoma 180 maintained in Tian-jin albino mice, four ascitic tumors including Sarcoma 180 maintained in Tian-jin albino and 615 strain mice, and Hepatoma and Ehrlich carcinoma maintained in C57BL mice. The results. showed that spleen PFC decreased as the tumors progressed,...
A modified method of Jerne's assay was used to determine haemolytic plaque-forming cells (PFC) in mouse spleen. By means of this method we measured the dynamic changes of the immune status in tumor-bearing mice. The tumors used were solid Sarcoma 180 maintained in Tian-jin albino mice, four ascitic tumors including Sarcoma 180 maintained in Tian-jin albino and 615 strain mice, and Hepatoma and Ehrlich carcinoma maintained in C57BL mice. The results. showed that spleen PFC decreased as the tumors progressed, and was hardly detected when the tumors were far advanced. The tumor-bearing mice commonly had striking splenomegaly with. decrease in number of small lymphocytes and. increase of 'immature' plasma cells, which might explain the poor immune responses of tumor-bearing mice.
It has been shown by haemolytic plaque-forming cell assay that there is an immunosuppressive factor(s) with high activity in the cell-free aseitic fluid of Sarcoma 130 maintained in 615 strain mice. The suppression is dose-dependent and becomes apparent only when the aseitic fluid is administered before immunization with sheep red blood cells. The immunosuppressive factor (s) seems partially heat-labile. The serum of the same tumor-bearing mice also possesses a strong immunosuppressive activity....
It has been shown by haemolytic plaque-forming cell assay that there is an immunosuppressive factor(s) with high activity in the cell-free aseitic fluid of Sarcoma 130 maintained in 615 strain mice. The suppression is dose-dependent and becomes apparent only when the aseitic fluid is administered before immunization with sheep red blood cells. The immunosuppressive factor (s) seems partially heat-labile. The serum of the same tumor-bearing mice also possesses a strong immunosuppressive activity. Furthermore, similar immunosuppressions were observed in normal recipient mice treated with cell-free aseitic fluids and sera of mice bearing some other strains of tumor including Hepatoma and Ehrlich carcinoma maintained in C57BL mice and Sarcoma 180 maintained in Tian-jin albino mice. Histopathologic changes of spleens induced by the cell-free aseitic fluid resembled those observed in spleens of tumor-bearing mice.
The spleen cells taken from Balb/ cJ mice 3 days after immunization with PDRBC were fused with NS-1 myelo-ma cells by two different procedures in the presence of PEG.The hybridcells secreting the specific antibody were selected out in the HAT medium by means of the spot test of the culture fluid.(Table 1).The antibody secre-ting hybrid cells were then cloned by ...
The spleen cells taken from Balb/ cJ mice 3 days after immunization with PDRBC were fused with NS-1 myelo-ma cells by two different procedures in the presence of PEG.The hybridcells secreting the specific antibody were selected out in the HAT medium by means of the spot test of the culture fluid.(Table 1).The antibody secre-ting hybrid cells were then cloned by either limiting dilution or micromani-pulation methods and a cell line thus obtained was designated as CBH-1.After ten months of culture in vitro up to 69 generations,the ability of this cell line to secret specific monoclonal antibody to PDRBC was not decreased. The CBH-1 cells were also ino-culated into the peritoneal cavity of Balb/cJ or Balb/cCr mice and could be kept in these mice for at least four transplantation generations.The ability of three different agents for inducing the ascitic fluid was compared.As a result,pristane was found to be superior to liquid paraffin and Arlacel A. Attempt was also made to cultureCBH-1 in serum-free medium instead of the conventional medium containing 20% newborn calf serum (NCS).When the serum-free medium was provided with 5γ transferrin per ml,the CBH-1 cells could be maintained just as well as that in DMEM+20% NCS within 15 days (Table 2) The proportion of plaque forming cells after the first cloning was found to be 97.5% and this was increased to 99.8% after second cloning (Table 3).This result implies that the cell popula-tion of the CBH-1 clone was very ho-mogeneous with respect to the secre-tion of the specific antibody to PDR-BC.
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