Twelve maize inbred lines were examined for RAPD analysis with 220 ten-mer Opron primers. Of them, 12 were selected for cluster analysis because they gave reproducible, polymorphic fingerprintings. Dendrogram constructed from RAPD data showed that twelve maize inbred lines were devided into three clusters. The first cluster consists of Huangzao-4 and four inbred lines whose one parent is Huangzao-4; 502-196 and 502 form the first subgroup since 502-196 is an improved inbred... Twelve maize inbred lines were examined for RAPD analysis with 220 ten-mer Opron primers. Of them, 12 were selected for cluster analysis because they gave reproducible, polymorphic fingerprintings. Dendrogram constructed from RAPD data showed that twelve maize inbred lines were devided into three clusters. The first cluster consists of Huangzao-4 and four inbred lines whose one parent is Huangzao-4; 502-196 and 502 form the first subgroup since 502-196 is an improved inbred line of 502; Huangzao-4 and Wenhuang-31413 form the second subgroup; H21 forms the third subgroup because its another parent is an American inbred line. The second cluster includes two inbred lines, 478 and 488, which came from a same F2 plat. The five remaining inbred lines form a separate cluster for their different origin; Mol7, a well-known American inbred line, forms the first subgroup; 107 and 81 12, which also came from the United States, form the second subgroup; 340 and 515 form the third subgroup, 340 is a native inbred line whose parents are Chinese local varieties and 515 has American and Chinese genealogy. These results indicate that phylogenetic relationship determined by RAPD analysis is basically consistent with the pedigree of the twelve maize inbred lines. |