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peroxidase
相关语句
  相似匹配句对
     Study on Imitation of Peroxidase
     过氧化物酶的模拟酶研究(M-TPPS_4-4-AAP-苯酚衍生物-H_2O_2体系)
短句来源
     the peroxidase isoenzyme varied.
     组织内过氧化物酶同工酶显著变化,并出现与盐离子胁迫有关的特异谱带.
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  peroxidase
Hepatic glutathione, lipid peroxides, glutathione peroxidase, alcohol dehydrogenase, aldehyde dehydrogenase, glycogen and total protein in liver were also significantly altered.
      
A novel amperometric hydrogen peroxide sensor was proposed by co-immobilizing new methylene blue (NMB) and Horseradish peroxidase (HRP) on glassy carbon electrode through covalent binding.
      
The activities of superoxide dismutase (SOD) and peroxidase (POD), and the content of malondialdehyde (MDA) were determined.
      
The activity of peroxidase (POD) decreases and it has no significant correlation with the tissue water content.
      
The manganese peroxidase (MnP) activity was 2.5 times greater than that produced in cultures without glucose feeding.
      
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A method for the localization of HBsAg in hepatocytes by means of horseradishperoxidase-labelled antibody(indirect method)at electron microscopic level isdescribed.Some problems involved are discussed.The ultrathin sections for electron microscopic examination may be preparedeither after re-embedding of a thick section used in light microscopic examination,in which HBsAg was localized immunocytochemically with horseradish peroxidase-labelled antibody,or by staining immunocytochemically with horseradish...

A method for the localization of HBsAg in hepatocytes by means of horseradishperoxidase-labelled antibody(indirect method)at electron microscopic level isdescribed.Some problems involved are discussed.The ultrathin sections for electron microscopic examination may be preparedeither after re-embedding of a thick section used in light microscopic examination,in which HBsAg was localized immunocytochemically with horseradish peroxidase-labelled antibody,or by staining immunocytochemically with horseradish peroxidase-labelled antibody directly on ultrathin section.The distribution and form of HBsAg in the HBsAg pesitive hepatocyte has beenexamined preliminarily,using horseradish peroxidase-labelled antibody under electronmicroscopy.Under electron microscopic examination there are some deposits of HBsAg withenzyme-labelled antibody,dispersed in the cytoplasma or the expanded cisternae ofthe endoplasmic reticulum of some hepatocytes which were verified light microscopicallyto be HBsAg negative.Electron microscopic examination gives more significantinformation.

本文介绍辣根过氧化物酶标记抗体对肝细胞内乙型肝炎表面抗原(HBsAg)的免疫电镜定位方法。电镜样品制备,可采用酶标免疫显色组织切片的再包埋、或直接利用超薄切片进行酶标免疫显色定位。对实验中应注意的一些问题进行了讨论。在电子显微镜下初步观察了HBsAg 阳性肝细胞中HBsAg 的分布和形态,并发现在光学显微镜下,辣根过氧化物酶标免疫定位HBsAg 完全为阴性部位的肝细胞中,有的细胞胞浆内或扩大的内质网池内,尚可见到少量散在的HBsAg 与酶标抗体的沉积物,提示电镜观察有更好的灵敏性。

The changes in peroxidase isozymes of different varieties of cotton seedlings after inoculation by Fusarium oxysporum f.sp.vasinfectum was studied with polyacrylamide gel electrophoresis.After inoculation,the activities of peroxidase isozymes in coty- ledons were intensified and new bands appeared.The enzyme activities of susceptible varities (4 varieties)were higher than those of the resistant ones (8 varieties).The susceptible varieties invariably had one or two more isozyme bands than the resistant...

The changes in peroxidase isozymes of different varieties of cotton seedlings after inoculation by Fusarium oxysporum f.sp.vasinfectum was studied with polyacrylamide gel electrophoresis.After inoculation,the activities of peroxidase isozymes in coty- ledons were intensified and new bands appeared.The enzyme activities of susceptible varities (4 varieties)were higher than those of the resistant ones (8 varieties).The susceptible varieties invariably had one or two more isozyme bands than the resistant ones.The changes in intensity of the peroxidase isozymes of cotton seedlings after infection is coincident with the appearance of morphological symptom of the disease. The number of isozymes increased with the severity of the disease,so that the changes in the number of isozymes in the infected plant can be taken as the intrinsic“bioche- mical symptom”of the plant.There were distinct differences among the three species of cotton plants in their peroxidase isozymes.The number of isozymes is greatest in Island cotton,which is highly susceptible to fusarial wilt disease,followed by the susceptible varieties of Continent cotton and its resistant varieties,while the least is the Chinese cotton which is immune to the disease.It is suggested that the appearauce of more new isozymes in the susceptible varieties upon inoculation may be used as a criterion for screening the resistant varieties of cotton plants from those susceptible to fusarial wilt disease.

利用聚丙烯酰胺凝胶电泳研究了棉花不同品种接种枯萎菌(Fusarium oxysporum f.sp.vasinfectum)后过氧化物酶同工酶的变化。接种后,感病品种(4个)比抗病品种(8个)的过氧化物酶同工酶的活性强,酶带数增多1—2条。这种变化与植株外部表现的症状相一致,即病情越重,酶带数越多。因此,感病植株的同工酶变化可以看作植株内部的“生化症状”。海岛棉、陆地棉和中棉之间,过氧化物酶同工酶存在着明显的差异。高度感染枯萎病的海岛棉同工酶最多(8条),其次为陆地棉的感病品种(7条),再次为陆地棉的抗病品种(6条),不感染枯萎病的中棉同工酶最少(4条)。由于抗病品种与感病品种过氧化物酶对枯萎菌侵染所作出的反应不同,因此,可以利用接种后感病品种比抗病品种产生新的酶带较多的特点,作为筛选棉花抗枯萎病品种的一种技术。

The results obtained from investigations in the field and laboratory showed that selenium deficiency plays an important role in the causation of Keshan disease. This conclusion is based on: ( 1 ) Oral administration of sodium selenite was highly effective in the prevention of Keshan disease. In Mianning county of Sichuan province, the incidence rate of Keshan disease in the selenium treated children were 2.2% and 1.0% in 1974 and 1975 respectively, while those of the control group were 13.5% and 9.5%, both differences...

The results obtained from investigations in the field and laboratory showed that selenium deficiency plays an important role in the causation of Keshan disease. This conclusion is based on: ( 1 ) Oral administration of sodium selenite was highly effective in the prevention of Keshan disease. In Mianning county of Sichuan province, the incidence rate of Keshan disease in the selenium treated children were 2.2% and 1.0% in 1974 and 1975 respectively, while those of the control group were 13.5% and 9.5%, both differences being statistically significant. Up to the end of 1977, 21 cases of this disease occurred in selenium treated children, only 3 of them died: whereas, 106 cases occurred in the control group, of which 53 were fatal. ( 2 ) Selenium content of blood, hair and staple cereals in the affected areas was regularly lower than that in non-affected areas. (3 ) Urinary loading test and whole blood glutathione peroxidase activity of residents in the affected areas were also indicative of deficiency in selenium. However there were also evidences to suggest that selenium deficiency is not the only factor involved in the etiology of the disease. There may be some other etiolgical factors.

本文报告了硒与克山病关系的初步研究结果。根据(1)口服亚硒酸钠对预防急型和亚急型克山病的发病有显著效果;(2)克山病病区人血、头发和粮食中硒含量普遍低于非病区;(3)尿硒负荷试验和全血谷胱甘肽过氧化物酶活性测定表明,克山病病区居民体内处于贫硒状态,认为硒缺乏对克山病发病有重要的、但不是唯一的作用。

 
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