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hbv genotypes
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  hbv基因型
     A survey on a cohort of 125 patients with HBV in and around Hangzhou City, Zhejiang Province showed the existence of the following HBV genotypes: A (0.8%), B (48%), C (40.8%), D (0.8%), mixed B and C (9.6%) and an absence of E and F genotypes.
     本文检测了浙江省杭州市及其周边地区125例HBV感染者其HBV基因型,结果如下:A型占0.8%,B型占48%,C型占40.8%,D型占0.8%,B、C混合型占9.6%,未检出E、F型。
短句来源
     the HBV e antigens were positive in 14/16 cases of genotype C and 12/22 cases of genotype B.10 samples which HBV DNA quantities were lower than 105 copies/ml couldn't be detected the HBV genotypes and YMDD mutations.
     B型和C型患者e抗原阳性率分别为54.5%(12/22)和87.5%(14/16)。 10份HBV DNA定量<1.0×105拷贝/毫升的标本不能检测出HBV基因型及YMDD变异情况。
短句来源
     A Study on HBV Genotypes and Selected HBV X Gene Mutations in Patients with HBV-Induced Liver Diseases
     HBV感染的肝病患者HBV基因型和HBV X 基因选择性突变的研究
短句来源
     Study on Detection of HBV Genotypes in Pregnant Women and It's Correlation with Intrauterine Infection
     妊娠妇女HBV基因型检测及其与宫内感染的相关性研究
短句来源
     Conclusion:HBV genotypes were mainly type C,and followed by type B and type D.
     结论 :本研究中乙肝病人HBV基因型主要为C型 ,其次为B型及D型。
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  hbv的基因型
     Methods Serum samples from 190 HBV DNA positive patients with chronic HBV infection, including 52 asymptomatic HBV carriers (ASC), 56 chronic hepatitis (CH), 32 fulminant hepatic failure (FHF), 22 liver cirrhosis (LC), and 28 hepatocellular carcinoma (HCC) patients were collected and tested for HBV genotypes by type specific primers.
     方法 选择湖北地区HBV DNA阳性的慢性HBV感染者190例,其中乙型肝炎表面抗原(HBsAg)携带者52例、慢性乙型肝炎56例、重型肝炎32例、肝硬化22例、原发性肝癌28例,应用多对型特异性引物-聚合酶链反应检测HBV的基因型
短句来源
     Methods 298 patients with HBV infection including asymptomatic HBV carriers(ASC),acute hepatitis(AH),chronic hepatitis(CH),severe hepatitis(SH),liver cirrhosis(LC),and hepatocellular carcinoma(HCC) were collected and tested for HBV genotypes by the multiplex type-specific primers PCR.
     方法选择298例HBV感染者,分成表面抗原携带者(ASC)、急性肝炎(AH)、慢性肝炎(CH)、重型肝炎(SH)、肝硬化(LC)和原发性肝癌(HCC)6组,用多对型特异性引物PCR方法检测HBV的基因型;
短句来源
     Methods: Serum samples from 215 HBV DNA positive patients with chronic HBV infection,including 3 asymptomatic HBV carriers(ASC),3 acute hepatitis(AH),166 chronic hepatitis(CH),(mild 80,moderate 50 severe36); 27 Liver cirrhosis(LC); 12 hepatocellular carcinoma(HCC) and 4 fulminant hepatic failure(FHF) patients were collected and tested for HBV genotypes by s-region gene sequencing.
     方法:选择江苏省HBV-DNA阳性慢性HBV感染者215例,其中HBsAg携带者3例,急性乙型肝炎3例,慢性乙型肝炎166例(轻度80例、中度50例、重度36例),肝硬化27例,原发型肝癌12例,重症肝炎4例,采用S区基因测序法检测HBV的基因型
短句来源
     Methods Serum sample from 257 patients with chronic HBV infection,including 49 asymptomatic HBV carriers(ASC),52 mild chronic hepatitis B,38 moderate chronic Hepatitis B,32 severe chronic hepatitis B,22 fulminant hepatitis failure(FHF),41 liver cirrhosis(LC),and 23 hepatocellular carcinoma(HCC) patients were collected and tested for HBV genotypes by polymerase chain reaction(PCR) micro-board nucleic acid hybridization-enzyme-linked-immunosorbent assay(ELISA).
     方法选择镇江地区HBV感染者257例,其中慢性HBV携带者49例,慢性乙型肝炎轻度52例、中度38例、重度32例,重型肝炎22例,肝硬化41例,原发性肝癌23例。 应用聚合酶链反应(PCR)微板核酸杂交-酶联免疫吸附(ELISA)技术检测HBV的基因型
短句来源
  乙肝病毒基因型
     Application of gene chip technique in the detection of HBV genotypes and YMDD mutations
     基因芯片技术在乙肝病毒基因型及YMDD变异检测中的应用
短句来源
     Influence of HBV Genotypes on Response to Antivirotic Therapy
     乙肝病毒基因型与抗病毒治疗应答的相关性研究
短句来源
     Objective :To compare the HBV genotyping method of direct sequencing of HBV DNA S gene with PCR microplate hybridization-ELISA technique, and to study the relationship between HBV genotypes and clinical outcome .
     目的:比较两种测定乙型肝炎病毒(hepatitis B virus, HBV)基因型方法的灵敏度、特异性、可行性及成本,探讨乙肝病毒基因型与临床疾病谱、疾病进展及抗病毒治疗疗效的关系。
短句来源
     Genotypes C is the major genotype in this area. Gene mutation exists in HBV genotypes.
     结论 江苏省泰州地区存在乙肝病毒基因型B和基因型C ,其中C型占优势 ;
短句来源
     Objective: To evaluate the influence of HBV genotypes on response to recombinant-interferon-alpha(r-IFN-α)therapy in chronic HBV DNA positive patients.
     目的 :旨在探讨乙肝病毒基因型对α 干扰素抗病毒治疗应答的影响。
短句来源
  “hbv genotypes”译为未确定词的双语例句
     There was no significant difference on HBV genotypes(χ~2=0.17,P>0.05).
     YMDD变异与基因型无明显相关性(χ2=0.17,P>0.05)。
短句来源
     Methods IL-2,IFN-γ,IL-4,IL-10 released by Th were measured in 29 patients with different HBV genotypes. Then the data was analyzed.
     方法检测29例不同基因型慢性乙肝患者外周血Th细胞因子(IL-2、IFNγ-、IL-4、IL-10)的表达,并作统计学分析。
短句来源
     Results:There were 30 cases in the 40 HCC patients whose HBV DNA quantification were positive, the average levels of the HBV DNA quantification were (6.53±1.31) copy/ml(Logarithm). The HBV genotypes and HBV mutants were tested for the 30 HCC and 40 CHB patients, the results showed that in the 30 HCC cases, 5(16.7%) were genotype B, 25 (83.3%) were genotype C;
     结果:40例HCC患者中有30例HBV DNA定量阳性,平均对数值为(6·53±1·31)copy/ml,将30例HBV DNA定量阳性的HCC及40例CHB患者的血清进行HBV基因分型及基因变异检测,结果显示30例HCC中HBV B基因型5例(16·7%),C基因型25例(83·3%);
短句来源
     Results In HBV genotypes,36% were type C,30% were type B and 23% were type D.
     结果 HBVC基因型占 36 % ,B基因型占 30 % ,D基因型占 2 3%。
短句来源
     Results Four HBV genotypes were detected in 76 patients: 47 patients with B, 21 with C, 3 with D and 5 were infected by genotype B mixed with C HBV infection.
     结果共检测到4种基因型:B (47例)、C(21例)、D(3例)和B/C混合(5例)。
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  hbv genotypes
The aim of this study was to reveal the HBV genotypes present in Hungary and to investigate a nosocomial and an intrafamilial outbreak.
      
Two HBV genotypes, A and D, were encountered in Serbia, with genotype D (D - 82%, A - 18%) and subgenotype D3 (47.9%) being prevalent.
      
The aim of this study was to gain insight into the distribution of HBV genotypes, subgenotypes and HBsAg subtypes among HBV chronically infected patients in Serbia, since there were no previously published data on this subject.
      
Distribution of HBV genotypes, subgenotypes and HBsAg subtypes among chronically infected patients in Serbia
      
Seven human hepatitis B virus (HBV) genotypes have been described.
      
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A unique hepatitis virus(HBV)variant has been identified previously in Chinese patients withchronic liver diseases, whose sera were positive for HBV DNA by dot blot hybridization but HBsAg nega-tive by conventional polyclonal antibody based immunoassays. In this study,the complete gnome of this u-nique variant from Chinese HBsAg negative but HBV DNA positive patients with chronic liver disease wassequenced. The gnome,adw subtype,consists of 3224 bp. When comparing the variant with wild type...

A unique hepatitis virus(HBV)variant has been identified previously in Chinese patients withchronic liver diseases, whose sera were positive for HBV DNA by dot blot hybridization but HBsAg nega-tive by conventional polyclonal antibody based immunoassays. In this study,the complete gnome of this u-nique variant from Chinese HBsAg negative but HBV DNA positive patients with chronic liver disease wassequenced. The gnome,adw subtype,consists of 3224 bp. When comparing the variant with wild type con-sensus sequences of an adW and an adr from same region,homologies vvere 98.0%and 92.5%respectively.Comparing the variant with the five known HBV genotypes A to E and censuses sequences previously pub-lished from same region,this isolate was an insertion variant with nine base pair insertion which introducedtwo additional amino acids between codons 122 to 124 immediately upstream of the″a″determinant of HB-sAg. There was no unique nucleoacid change in promoter regions(SPI,SP2,XP and CP)and enhancer re-gions(ENH1 and ENH2). Dendrogram based on the nucleotide sequences of complete standard HBVgnomes usingthe whole genome for alignment including 4 Chinese isolates was constructed.

用核酸序列分析技术,测定1例乙型肝炎表面抗原(HBsAg)阴性中国人感染的乙型肝炎病毒HBV/adw亚型全基因组序列。该变异株全序列为3224个碱基,与从同一地区2例HBsAg阳性携带者得到的adw和adr全序列的同源性分别为98.0%和92.5%,与已出版的A—E基因组比较,进行了病毒的系统树分析。该变异毒株除在外膜基因A决定簇前有9个碱基插入外,在多种调控元件,包括启动子SPI、SPII、CP、XP、增强子ENI和ENII,均无独特的变异点。

Prevalence of hepatitis B virus(HBV) genotypes in patients with chronic HBV infection has been investigated in Ning Xia area of China by using polymerase chain reaction (PCR) amplification in combination with a restriction fragment length polymorphism (RFLP) assay. HBV genotyping following PCR amplification of pre S fragment was carried out in 90 serum samples from HBeAg positive patients. The genotype C was detected in 86.7%(78/90) of subjects, genotype D in 10%(9/90), undefined genotype...

Prevalence of hepatitis B virus(HBV) genotypes in patients with chronic HBV infection has been investigated in Ning Xia area of China by using polymerase chain reaction (PCR) amplification in combination with a restriction fragment length polymorphism (RFLP) assay. HBV genotyping following PCR amplification of pre S fragment was carried out in 90 serum samples from HBeAg positive patients. The genotype C was detected in 86.7%(78/90) of subjects, genotype D in 10%(9/90), undefined genotype in 3.3%(3/90). There is no genotypes of A, B,E and F in this group of patients. This result shows that HBV genotype C was the predominant genotype in Ning Xia area, and genotype D was a newly described genotype in this region. This finding provides a new information for understanding the distribution of HBV genotype in China.

为确定乙型肝炎病毒 (HBV) D基因型在我国的存在 ,建立我国 HBV D基因型的参照序列 ,作者用聚合酶链式反应 (PCR)结合限制性片段长度多态性分析 (RFL P)技术 ,调查了可能存在 D基因型的我国宁夏地区 HBV基因型分布。对 90例 HBe Ag阳性 HBV感染者的血清进行前 S区 RFL P基因型分型。结果发现 :C基因型 86 .7% (78/ 90 ) ;D基因型 1 0 % (9/ 90 ) ;无 A、B、E和 F型 ;不能明确分型者 3.3% (3/ 90 )。 2例 RFL P分型为 D基因型的病例经测序证实。结果表明 ,宁夏地区 HBV优势基因型为 C型 ,并首次在该地区发现 D基因型的存在。本研究为我国 HBV基因型分布积累了新的资料 ,为进一步建立我国 HBV D基因型的参照序列提供了基础

Objective To determine the distribution and genetic relationship of hepatitis B virus (HBV) genotypes and subtypes. Methods HBV genotypes and subtypes were determined by PCR and DNA sequencing among 280 chronic HBV carriers in 25 counties of 4 provinces (Hunan, Guangxi, Henan and Hebei) in China. Results Genotype B, C and D were detected in these regions. Genotypes C and B were the majority genotypes of HBV with 190 out of 280 ( 67.9 %) genotype C, 82 ( 29.3 ...

Objective To determine the distribution and genetic relationship of hepatitis B virus (HBV) genotypes and subtypes. Methods HBV genotypes and subtypes were determined by PCR and DNA sequencing among 280 chronic HBV carriers in 25 counties of 4 provinces (Hunan, Guangxi, Henan and Hebei) in China. Results Genotype B, C and D were detected in these regions. Genotypes C and B were the majority genotypes of HBV with 190 out of 280 ( 67.9 %) genotype C, 82 ( 29.3 %) genotype B, and 8 ( 2.9 %) genotype D. Adr, adw2, ayr, ayw1, ayw2 and ayw3 subtypes were determined among these carriers. Adr and adw2 subtypes were the leading subtypes of HBV,taking up 64.3 % and 31.4 %, respectively.Adr subtype was completely encoded by genotype C while majority of adw2 subtype was encoded by genotype B.An average rate nucleotide substitutions of 2.94 was seen among 280 Chinese HBV sequences. The average rate of nucleotide substitutions of genotype B (adw2 subtype) was 5.63 ( 5.48 ), but only 1.6 ( 1.51 ) for genotype C (adr subtype). Conclusion The results suggested that there were significant differences in geographic distribution of HBV genotypes and subtypes; genotype B, in which mostly consistent with adw2 subtype, was a higher variable than genotype C (adr subtype).

目的 研究乙型肝炎病毒 (hepatitisBvirus ,HBV)基因型和血清亚型的分布特点及其基因的相关性。方法 湖南、广西、河南和河北 4省 (区 ) 2 5个县 (市 ) 2 80例慢性HBV携带者 ,应用聚合酶链反应 (PCR)扩增和脱氧核糖核酸 (DNA)序列分析 ,确定HBV基因型和血清亚型。结果 HBV基因型B、C和D均有分布 ,分别占 2 9.3%、6 7.9%和 2 .9% ,其中B和C为优势基因型。adr、adw2、ayr、ayw1、ayw2和ayw3等 6种血清亚型均有分布 ,其中adr和adw2为优势血清亚型 ,分别占6 4.3%和 31.4%。基因型B与adw2血清亚型 ,基因型C与adr血清亚型有非常密切的基因相关性。 2 80例HBV携带者表面抗原基因序列每 10 0个核苷酸的平均置换频数为 2 .94。基因型B(adw2血清亚型 )毒株核苷酸置换频数为 5 .6 3(5 .48) ,而基因型C(adr血清亚型 )仅为 1.6 (1.5 1)。结论 HBV基因型和血清亚型的分布均有明显的地区性 ;基因型B及相对应的adw2血清亚型毒株的基因可变性明显高于基因型C及相应的adr血...

目的 研究乙型肝炎病毒 (hepatitisBvirus ,HBV)基因型和血清亚型的分布特点及其基因的相关性。方法 湖南、广西、河南和河北 4省 (区 ) 2 5个县 (市 ) 2 80例慢性HBV携带者 ,应用聚合酶链反应 (PCR)扩增和脱氧核糖核酸 (DNA)序列分析 ,确定HBV基因型和血清亚型。结果 HBV基因型B、C和D均有分布 ,分别占 2 9.3%、6 7.9%和 2 .9% ,其中B和C为优势基因型。adr、adw2、ayr、ayw1、ayw2和ayw3等 6种血清亚型均有分布 ,其中adr和adw2为优势血清亚型 ,分别占6 4.3%和 31.4%。基因型B与adw2血清亚型 ,基因型C与adr血清亚型有非常密切的基因相关性。 2 80例HBV携带者表面抗原基因序列每 10 0个核苷酸的平均置换频数为 2 .94。基因型B(adw2血清亚型 )毒株核苷酸置换频数为 5 .6 3(5 .48) ,而基因型C(adr血清亚型 )仅为 1.6 (1.5 1)。结论 HBV基因型和血清亚型的分布均有明显的地区性 ;基因型B及相对应的adw2血清亚型毒株的基因可变性明显高于基因型C及相应的adr血清亚型。

 
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