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restriction endonucleases patterns
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  相似匹配句对
     THE RESTRICTION ENDONUCLEASES FROM BACILLUS
     芽孢杆菌中的限制性核酸内切酶
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     Analysis of the Chromosome Banding with Restriction Endonucleases
     用限制性内切酶诱导染色体显带的分析
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     On Restriction of Ownership
     论所有权的限制
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     Promotion and Restriction
     发展与制约
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     The digest patterns of restriction endonucleases were in conformity with the predicted sizes completely.
     自溶素及溶血素基因的扩增产物分别经限制性内切酶TthHB81和AccI消化后产生的片段和预期的完全一致 ;
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EpNPV-m (Euproctis pseudoconspersa Nuclear Pojyhedrosis Virus Meng-mountain Strain) Contains ds DNA, The amount of the DNA per mg polyhedral included bodies is 6, 85ug, The u Itraviolet absorption curve of the DNA purified by phenol-cnloroform extraction is typical. There is the same size among the DNA molecular Tm of the DNA in PH8.0 TE buffer is 68.9℃(G+C)% is about 36,6 Numbers of the restriction fragments generated by cleavage with EcoRI, BamHI, Bgl II are 27, 9,15 respectively. The restriction endonucleases...

EpNPV-m (Euproctis pseudoconspersa Nuclear Pojyhedrosis Virus Meng-mountain Strain) Contains ds DNA, The amount of the DNA per mg polyhedral included bodies is 6, 85ug, The u Itraviolet absorption curve of the DNA purified by phenol-cnloroform extraction is typical. There is the same size among the DNA molecular Tm of the DNA in PH8.0 TE buffer is 68.9℃(G+C)% is about 36,6 Numbers of the restriction fragments generated by cleavage with EcoRI, BamHI, Bgl II are 27, 9,15 respectively. The restriction endonucleases patterns are made, The average molecular weight estimated by accumulating the restriction fragments is 75,61×106i109,75Kb, The molecular length measured on electron microscopic photographes of the DNA is about 35,Sum, approximately 74, 1×106,109,57Kb, Some suppercoil circuler, molecules were observed by electron microscopy.

本文报道了对茶毛虫核型多角体病毒蒙山株系(EpNPV-M)核酸性质研究的结果。EpNPV-M含双链DNA分子,含量为6.85μgDNA/mgPIB,提纯的DNA具典型的紫外线吸收特性,琼脂糖凝胶电泳证明DNA分子是大小均一的。DNA的(G+C)%为36.6,限制性片段积加法测得该DNA分子量为75.61×10~6道尔顿,109.57Kb,电镜法测得DNA分子长度为35.8μm,相当于分子量为74.1×10~6道尔顿,107.4Kb.电镜观察证实该病毒含有一些超螺旋环状DNA分子。建立了三种限制性内切酶对该DNA的酶切图谱。三种酶的酶解片断数为:EcoRI,27个;BglⅡ,15个;BamHI,9个。

Objective To explore the possibility of plasmid (pR ST98 ) encoding mult resistance to antimicrobial agents in S.typhi carrying the Salmonella plasmid virulence (spv) gene. Methods Plasmid pR ST98 was isolated by QIAGEN kit. Eight restriction endonucleases were selected to make the pR ST98 fingerprint. spv specific PCR and Southern blot with a spv gene probe prepared from plasmid pGTR061 were applied to identify the virulence gene on pR ST98 . Results The fragments...

Objective To explore the possibility of plasmid (pR ST98 ) encoding mult resistance to antimicrobial agents in S.typhi carrying the Salmonella plasmid virulence (spv) gene. Methods Plasmid pR ST98 was isolated by QIAGEN kit. Eight restriction endonucleases were selected to make the pR ST98 fingerprint. spv specific PCR and Southern blot with a spv gene probe prepared from plasmid pGTR061 were applied to identify the virulence gene on pR ST98 . Results The fragments of pR ST98 digested by endonucleases BglⅡ, PstⅠand SacⅡ were well identified and therefore, may be very useful tools for molecular analysis and further epidemiological surveillance of pR ST98 . The results of PCR and Southern blot showed that spv homologous genetic sequence which had been found in all other pathogenetic Salmonella spp . except S.typhi was also present on pR ST98 . Conclusion The restriction endonucleases pattern of pR ST98 is potential in character molecular markings, epidemiological surveillance and analysis of the functional genes of the plasmid. The genotype research of pR ST98 revealed that a plasmid carrying genes coding for drug resistance and virulence in S.typhi . PCR and Southern blot results presented in this study demonstrated the presence of a mosaic like structure for pR ST98 . [

目的 研究多重耐药伤寒沙门菌耐药质粒pRST98是否具有沙门菌属其他致病菌毒力质粒上的保守基因spv。方法 用QIAGEN试剂盒提取pRST98,精确定量后选用 8种限制性核酸内切酶消化 ,建立该质粒的酶切图谱。用spv特异引物的PCR扩增和spv探针Southernblot法分析pRST98的基因构成。结果 pRST98酶切图谱显示BglⅡ、PstⅠ和SacⅡ作用后片段均一 ,是对该质粒进行分子生物学和分子流行病学研究的理想工具。PCR和Southernblot结果显示 ,伤寒杆菌耐药质粒pRST98上存在其他沙门菌毒力质粒上高度保守基因spv的同源序列。结论 从基因水平首次证实伤寒杆菌耐药质粒pRST98具有多效性 ,是既编码抗药性又编码细菌毒力的“嵌合型”质粒。

 
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