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   mannan-binding lectin 在 基础医学 分类中 的翻译结果: 查询用时:0.012秒
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mannan-binding lectin
相关语句
  甘露聚糖结合凝集素
    Regulatory Roles of Mannan-binding Lectin in Differentiation and Functions of Antigen Presenting Cells
    甘露聚糖结合凝集素对抗原提呈细胞分化与功能的调节作用
短句来源
    Construction of CGT52TGT and GGA57GAA mutants of mannan-binding lectin gene
    CGT52TGT和GGA57GAA甘露聚糖结合凝集素突变体的构建
短句来源
    The relationship between gene polymorphisms and serum protein level of mannan-binding lectin
    甘露聚糖结合凝集素基因多态性与血清蛋白表达水平的关系
短句来源
    The Direct Effects of Human Mannan-binding Lectin Carbohydrate-recognition Domain on Pathogens
    人甘露聚糖结合凝集素糖识别域对病原体的直接效应研究
短句来源
    Mannan-binding lectin (MBL), one of members of the collectin (C-type lectin with a collagen-like domain) family in the superfamily of C-type lectins, is a central component of the innate immune system.
    甘露聚糖结合凝集素(mannan-binding lectin,MBL)系C型凝集素超级家族中胶凝素家族成员,是天然免疫系统中的关键分子。
短句来源
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  甘露糖结合凝集素
    Background and Objective: Mannan-binding lectin (syn: mannose-binding lectin, MBL) is a Ca2+-dependent (C-type) serum lectin. It has an important role in the innate immune system against pathogenic microorganisms, especially for children of 6 months to 2 years old. MBL is an oligomer comprised of several identical subunits.
    研究背景与目的:甘露聚糖结合凝集素(mannan-binding lectin,MBL),又称甘露糖结合凝集素(mannose-binding lectin,MBL),是一种钙离子依赖的(C型)血清凝集素,在防御抵抗多种病原微生物的天然免疫中起着重要的作用,对6个月到2岁的婴幼儿尤其重要。
短句来源
  “mannan-binding lectin”译为未确定词的双语例句
    Construction of GGC54GAC mutant of mannan-binding lectin
    GGC54GAC MBL突变体的构建
短句来源
    Frequency of GGC54GAC point mutation of mannan-binding lectin gene in a Han population from Guangdong
    广东地区汉族人MBL基因GGC54GAC点突变的初步筛查
短句来源
    Expression, purification, and identification of the carbohydrate-recognition domain of human mannan-binding lectin in Escherichia coli
    人MBL糖识别域在大肠杆菌中的表达、纯化及鉴定
短句来源
    Effects of mannan-binding lectin on TNF-α and IL-12 production induced by lipopolysaccharide in differentiated and activated THP1/CD14 cells
    MBL对LPS刺激的不同分化和活化状态THP1/CD14细胞产生TNF-α和IL-12的影响
短句来源
    AIM: To explore preliminarily the mechanisms of immunodeficiency resulted from the CGT52TGT point mutation of mannan-binding lectin(MBL) gene.
    目的: 初步探索MBL基因CGT52TGT点突变引起调理吞噬缺损的机制。
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  mannan-binding lectin
Possible disease-modifying factors: the mannan-binding lectin pathway and infections in hereditary angioedema of children and ad
      
Mannan-binding lectin (MBL), a human plasma protein, plays an important role in the innate immune defence.
      
Phase I Safety, Tolerability, and Pharmacokinetic Study of Recombinant Human Mannan-Binding Lectin
      
Mannan-binding lectin (MBL) is an activator of the complement system, and Apolipoprotein A-1 (Apo A-1) is pivotal in the cholesterol homeostasis and has anti-inflammatory properties.
      
Mannan-binding lectin (MBL) is the central protein in the activation of complement through the lectin pathway.
      
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The intact cDNA encoding the mannan binding lectin polypeptide including the signal sequence was isolated by using RT PCR method with total RNA extracted from fresh liver tissue of a Han nationality foetus, linked into pGEM T vector and transformed into E.coli TG1. The result of sequencing indicated that the sequence of our cDNA is different greatly from that of the reported human MBL cDNA but identical with that of the mRNA for...

The intact cDNA encoding the mannan binding lectin polypeptide including the signal sequence was isolated by using RT PCR method with total RNA extracted from fresh liver tissue of a Han nationality foetus, linked into pGEM T vector and transformed into E.coli TG1. The result of sequencing indicated that the sequence of our cDNA is different greatly from that of the reported human MBL cDNA but identical with that of the mRNA for human MBL in GenBank except two nuc leotides, and that its encoded product consists of a typical hydrophobic signal sequence of 20 amino acids and a mature MBL polypeptide of 228 amino acids.

从中国汉族人胎肝组织提取总RNA,以RT-PCR方法获取了编码含信号顺序的全长甘露聚糖结合凝集素(MBL)肽链的cDNA片段,将其与pGEM-T载体连接,转化大肠杆菌TG1,建立了MBL的cDNA克隆。序列分析表明:与文献报道的人MBLcDNA序列比较,差异颇大,但与GenBank中的人MBLmRNA比较,仅2个位置的核苷酸不同;其编码产物是20个残基的信号顺序和228个残基的成熟MBL多肽。

Objective To obtain the gene fragment encoding human mannan-binding lectin (MBL)-associated serine protease-2 (MASP-2). Methods The target cDNA fragment was amplified from the total RNA extracted from human fetal liver tissue by RT-nested PCR, cloned into pGEM-T vector and identified by restriction analysis and sequencing. Results The cDNA fragment encoding MASP-2 polypeptide with the signal sequence was isolated, linked with pGEM-T vector and transformed into E.coli TGI. The restriction maps of the selected...

Objective To obtain the gene fragment encoding human mannan-binding lectin (MBL)-associated serine protease-2 (MASP-2). Methods The target cDNA fragment was amplified from the total RNA extracted from human fetal liver tissue by RT-nested PCR, cloned into pGEM-T vector and identified by restriction analysis and sequencing. Results The cDNA fragment encoding MASP-2 polypeptide with the signal sequence was isolated, linked with pGEM-T vector and transformed into E.coli TGI. The restriction maps of the selected clones were consistent with those generated by computer analyses. The result of sequencing of one selected clone showed that its sequence was identical with that of reported human MASP-2 cDNA. Conclusion The human MASP-2 gene was successfully cloned, which prepares the ground for studying the molecular mechanisms by which MBL activates the complement lectin pathway and the mutations of MBL gene cause immunodeficiency.

目的 获得人甘露聚糖结合凝集素相关丝氨酸蛋白酶-2(MASP-2)编码区基因。方法采用 RT-巢式 PCR技术从 人胎肝组织总RNA扩增目的cDNA片段,克隆人pGEM-T载体,酶切图谱分析和测序鉴定。结果获得了编码合信号 顺序的MASP-2肽链全长cDNA,将其与pGEM-T载体连接,转化大肠杆菌TG1,建立了MASP-2的重组克隆。酶切图 谱与微机分析结果无异;序列分析表明,与报道的人 MASP-2cDNA序列一致。结论成功克隆了人 MASP-2基因,为深 入研究补体凝集素途径的激活机制和甘露聚糖结合凝集素基因突变引起免疫缺损的发病机制奠定了基础。

Objective:To explore the mechanisms of mannan-binding lectin(MBL) deficiency,the GGC54GAC MBL mutant was constructed.Methods:The mutant was produced by the megaprimer PCR method for site-directed mutagenesis of wild-type MBL cDNA of Chinese,cloned into pGEM-T vector and identified by restriction analysis and sequencing.Results:With wild-type MBL cDNA as template and Deep Vent DNA polymerase,the first round of PCR reaction was performed using 5'-primer and the internal mismatch primer,which destroys a Ban...

Objective:To explore the mechanisms of mannan-binding lectin(MBL) deficiency,the GGC54GAC MBL mutant was constructed.Methods:The mutant was produced by the megaprimer PCR method for site-directed mutagenesis of wild-type MBL cDNA of Chinese,cloned into pGEM-T vector and identified by restriction analysis and sequencing.Results:With wild-type MBL cDNA as template and Deep Vent DNA polymerase,the first round of PCR reaction was performed using 5'-primer and the internal mismatch primer,which destroys a Ban I site in code 54 of wild-type MBL gene,and a product of about 180 bp was obtained.Then,the second round amplification was carried out using the fragment of 180 bp and 3-primer,producing a DNA fragment of about 780 bp.The last product of PCR was inserted into pGEM-T vector and a selected clone was identified by restriction analysis with Ban I and sequencing to show the expected mutation without any other change in the full length human MBL cDNA.Conclusion:The GGC54GAC MBL mutant was successfully constructed,which lays a foundation for investigating the mechanisms by which the GGC54GAC mutation of MBL gene causes immunodeficiency and the relation between structure and function of MBL molecule.

目的 :在人甘露聚糖结合凝集素 (MBL)基因中引入GGC5 4GAC点突变。方法 :设计上下游引物和诱变引物 ,以汉族人野生型MBLcDNA为模板 ,采用megaprimer PCR技术进行定点突变 ,将PCR产物克隆至pGEM T载体 ,酶切和测序分析。结果 :以上游引物和诱变引物进行第一轮PCR ,获得一约 180bp的DNA片段 ,以此片段和下游引物行第二轮PCR扩增 ,得到一长约 780bp的产物 ;将其克隆至pGEM T载体 ,酶切发现第 5 4位密码中的BanI酶切位点已被破坏 ,与计算机酶切图谱分析结果一致 ;序列分析表明 ,除第 5 4位密码变为GAC外 ,余与野生型MBLcDNA完全相同。结论 :构建成功了GGC5 4GACMBL突变体 ,为深入探索MBL基因突变引起调理吞噬缺损的机制提供了分子病理模型

 
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