METHODS: Monocytes were isolated from normal human peripheral blood cells by density gradient centrifugation and the plastic-adherent cells were cultured with the cytokines ( rhGM-CSF , rhIL-4, rhTNF-α).
Objective:To evaluate the effects of lipopolysaccharide(LPS) and Staphylococcus aureus(SAC) on IL-23 and IL-12 subunits expression in normal human peripheral blood mononuclear cells (PBMC) ,and explored the regulation mechanisms.
In this study,by means of immunofluorescence label,flow cytometer,()~3H-TdR incorporation and MTT analysis,the immune function of dendritic cells(DCs) and T cells derived from normal human peripheral blood were investigated after X irradiation in different doses.
AIM: To study the effects of morphine on tumor necrosis factor α (TNF α) and interleukin 6 (IL 6) production in human whole blood and the possible mechanism.
The genetic engineering expression bacterial hIL13pGEX4T2/TG1 was successfully constructed through the insertion of hIL13 fragment(0.3 kb) that was directly amplified from activated healthy human peripheral blood mononuclear cells(HPBMC) by RTPCR method,into the GST fusion expression vector pGEX4T2,and the transformation into competent E.
【Objective】To research the in vitro proliferation and Th1 type cytokine secretion effect of human peripheral blood mononuclear cells(PBMC) activated by Ag85B/IL-2 fusion protein.
To establish a method to expand the natural killer T cells (NKT) in vitro and to investigate its functions, the TCRVα24+/Vβ11+ NKT cells from human peripheral blood mononuclear cells (MNC) and purified T lymphocytes were expanded by several kinds of methods.
ObjectiveTo develop a Nested-Real-Time-PCR method for the detection of F0XP3mRNA in human peripheral blood mononuclear cells(PBMC), study the correlation of F0XP3mRNA with CD4~+CD25~+ Regulatory T cell, ratio of CD4 and CD8, Th1 and Th2, syndrome types of Traditional Chinese Medicine(TCM).
A synthetic peptide was shown to bind to TACE and to inhibit TNF-α release from lipopolysaccharide (LPS)-stimulated human peripheral blood mononuclear cells (PBMC) by up to 60.3%.
To study the endocytic activity of dendritic cells (DCs) by obtaining fusion protein HSP70-EGFP as exogenous antigen and loading it with DCs derived from human peripheral blood.
The effect of lead, copper, mercury, and cadmium ions on peroxidase system activity in human peripheral blood neutrophils was studied using cytochemical methods.
Effects of chorionic gonadotropin (CG), estradiol, progesterone, and their physiological combinations on apoptosis of human peripheral blood T-lymphocytes were studied.
Human LAK cells were prepared by culturing normal human peripheral blood mononuclear cells (PBMC) with or without rIL-2 and assayed for T cell surface markers as well as anti-tumor activity against PGin vitro and in nude mice.
(3) EIF4E, VEGF and MMP-9 were expressed at high levels in Raji cells as compared to normal human peripheral blood monocular cells (NHPMC), and blockage of EIF4E expression brought down the expression of VEGF and MMP-9.
Our study showed that deguelin inhibited the proliferation of K562 cell and K562/ADM cell in a time- and dose-dependent manner and had minimal effects on normal human peripheral blood mononuclear cells.
Gp120 as a sole agent significantly suppressed IFN-γ production by mitogen-stimulated PBLs and induced the production of IFN-α in cultures of normal human peripheral blood mononuclear cells (PBMCs).
As different experimental models the leucocytes of the normal human peripheral blood have been used taking into account the relatively condensed chromatin of granulocytes with respect to that of the mononuclear cells.
However, with human whole blood, positive effects were seen using TCDO together with zymosan as well as specific and non-specific opsonizedKlebsiella pneumoniae K 17.
However, with human whole blood, positive effects were seen using TCDO together with zymosan as well as specific and non-specific opsonizedKlebsiella pneumoniae K 17.
These results correlate with observations from timekill curve studies in human whole blood, where ciprofloxacin showed superior bactericidal activity compared to norfloxacin or ofloxacin.
Organosphosphate pesticides have been found extractable by headspace solid-phase microextraction (SPME), and the best conditions of their extraction from human whole blood and urine samples have been investigated.
A synthetic peptide was shown to bind to TACE and to inhibit TNF-α release from lipopolysaccharide (LPS)-stimulated human peripheral blood mononuclear cells (PBMC) by up to 60.3%.
Human LAK cells were prepared by culturing normal human peripheral blood mononuclear cells (PBMC) with or without rIL-2 and assayed for T cell surface markers as well as anti-tumor activity against PGin vitro and in nude mice.
Our study showed that deguelin inhibited the proliferation of K562 cell and K562/ADM cell in a time- and dose-dependent manner and had minimal effects on normal human peripheral blood mononuclear cells.